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The map label for this gene is bioH [H]

Identifier: 110835079

GI number: 110835079

Start: 2531094

End: 2531906

Strand: Reverse

Name: bioH [H]

Synonym: ABO_2218

Alternate gene names: 110835079

Gene position: 2531906-2531094 (Counterclockwise)

Preceding gene: 110835080

Following gene: 110835078

Centisome position: 81.15

GC content: 57.07

Gene sequence:

>813_bases
ATGCCCAGGTTGATCCCTTTTCATAACACTTATCGGGCCACCGGTGTTGATGATGCCCAAGCCCAGGACATTGTTCTGAT
TCATGGCTGGGGCCTACATGCCATTGTCTTTGATGATATTGTGCCGGCCTTGCTAGCGCACTTTCGCGTCACCGTGGTGG
ATTTACCCGGCATGGGACAGAGCCCCTTGCCTAATGATGATTACAGCCTGGATTTTCTGGCCGAACAGGTGCTGGCGATT
ATGCCGCAGAAGGCCCATTTGCTGGGCTGGTCGTTGGGTGGGTTGGTGGCATTGGCCATGGCAGAGAAGGCGCCGGAGCG
GGTGCAATCGGTGGTGACGGTGGCGACTTCCCCGCGATTTACCGCCGCAGACGATTGGGCTCCCGCCATGAAGCCGGAGA
TTCTGGCCAAGTTCGCCGAGATGTTTAATGAAGACAATGAAGGCACGCTGGTCCGTTTTCTGGCGCTCAATTGCAAGGGC
AGTGCGGCCATGCGTGAGGATACTGCGCGTCTCAAACAGATTTTGTATTTCTGTGGTTTGCCGGCGCCGAGAGCCCTGCG
CGGCGGGTTGAACATCCTGCGCGACAGCGACTTGCGTGAGTCATTAACTGCGTTGTCTATGCCGGTGCTGATGGTGTTCG
GTGAACATGATCATATCGTGCCGGCGGCAGTCATGGCCGCGGTGGAGCCGTTGATTGGTAATGGCCGCACGGCCTTGATT
GAGCAGGTGGCCCATGTACCCTTCCTGTCGACCCCGGACACCTTTACCCAGGCCGTGTATGACTTTTATCGCGACTATCA
GTTGGTCCGCTGA

Upstream 100 bases:

>100_bases
TGCTGGCGGCGCTGGCGCAATGCCAAACCCAAGACGTTTAAATCACAGACTTTTTACCACCGAGATCGTTGTTTCTTCTG
TGGCAAAAAAAGGAAGATGA

Downstream 100 bases:

>100_bases
GGCGCCCGTGGATACTCCCGTGGAGCGCTCAGGAAAGGCGGTAGTGAGCCAGCACTTTGGCCGAGCTGCGCGTAGTTATG
ATGAACACGCCGTGCTGCAG

Product: biotin biosynthesis protein bioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPRLIPFHNTYRATGVDDAQAQDIVLIHGWGLHAIVFDDIVPALLAHFRVTVVDLPGMGQSPLPNDDYSLDFLAEQVLAI
MPQKAHLLGWSLGGLVALAMAEKAPERVQSVVTVATSPRFTAADDWAPAMKPEILAKFAEMFNEDNEGTLVRFLALNCKG
SAAMREDTARLKQILYFCGLPAPRALRGGLNILRDSDLRESLTALSMPVLMVFGEHDHIVPAAVMAAVEPLIGNGRTALI
EQVAHVPFLSTPDTFTQAVYDFYRDYQLVR

Sequences:

>Translated_270_residues
MPRLIPFHNTYRATGVDDAQAQDIVLIHGWGLHAIVFDDIVPALLAHFRVTVVDLPGMGQSPLPNDDYSLDFLAEQVLAI
MPQKAHLLGWSLGGLVALAMAEKAPERVQSVVTVATSPRFTAADDWAPAMKPEILAKFAEMFNEDNEGTLVRFLALNCKG
SAAMREDTARLKQILYFCGLPAPRALRGGLNILRDSDLRESLTALSMPVLMVFGEHDHIVPAAVMAAVEPLIGNGRTALI
EQVAHVPFLSTPDTFTQAVYDFYRDYQLVR
>Mature_269_residues
PRLIPFHNTYRATGVDDAQAQDIVLIHGWGLHAIVFDDIVPALLAHFRVTVVDLPGMGQSPLPNDDYSLDFLAEQVLAIM
PQKAHLLGWSLGGLVALAMAEKAPERVQSVVTVATSPRFTAADDWAPAMKPEILAKFAEMFNEDNEGTLVRFLALNCKGS
AAMREDTARLKQILYFCGLPAPRALRGGLNILRDSDLRESLTALSMPVLMVFGEHDHIVPAAVMAAVEPLIGNGRTALIE
QVAHVPFLSTPDTFTQAVYDFYRDYQLVR

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=234, Percent_Identity=43.1623931623932, Blast_Score=177, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 29648; Mature: 29516

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLIPFHNTYRATGVDDAQAQDIVLIHGWGLHAIVFDDIVPALLAHFRVTVVDLPGMGQ
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEEEECCCCCC
SPLPNDDYSLDFLAEQVLAIMPQKAHLLGWSLGGLVALAMAEKAPERVQSVVTVATSPRF
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TAADDWAPAMKPEILAKFAEMFNEDNEGTLVRFLALNCKGSAAMREDTARLKQILYFCGL
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
PAPRALRGGLNILRDSDLRESLTALSMPVLMVFGEHDHIVPAAVMAAVEPLIGNGRTALI
CCCHHHHHCHHHHCCCHHHHHHHHHHHCHHEEECCCCCHHHHHHHHHHHHHHCCCHHHHH
EQVAHVPFLSTPDTFTQAVYDFYRDYQLVR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PRLIPFHNTYRATGVDDAQAQDIVLIHGWGLHAIVFDDIVPALLAHFRVTVVDLPGMGQ
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEEEECCCCCC
SPLPNDDYSLDFLAEQVLAIMPQKAHLLGWSLGGLVALAMAEKAPERVQSVVTVATSPRF
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TAADDWAPAMKPEILAKFAEMFNEDNEGTLVRFLALNCKGSAAMREDTARLKQILYFCGL
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCC
PAPRALRGGLNILRDSDLRESLTALSMPVLMVFGEHDHIVPAAVMAAVEPLIGNGRTALI
CCCHHHHHCHHHHCCCHHHHHHHHHHHCHHEEECCCCCHHHHHHHHHHHHHHCCCHHHHH
EQVAHVPFLSTPDTFTQAVYDFYRDYQLVR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA