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The map label for this gene is amiB [H]

Identifier: 110835067

GI number: 110835067

Start: 2518664

End: 2520019

Strand: Reverse

Name: amiB [H]

Synonym: ABO_2206

Alternate gene names: 110835067

Gene position: 2520019-2518664 (Counterclockwise)

Preceding gene: 110835068

Following gene: 110835066

Centisome position: 80.77

GC content: 55.75

Gene sequence:

>1356_bases
ATGACAATGCGGAAAACTGTAGCGGTAGCTTTTCTTTCTCTGTTGGCTTTGTGCTGGAGCTCACTGGTGTTTGCCCAGAC
CACGATTGATTCCGTACGCCTGCACCGGGCTCCGGATCACACTCGGATCGTATTCGATCTGCCCGGCCCGGTGGACCACA
AGCTCGACAAGCTGGCCAACCCGGATCGTATTGTGTTGGATCTGCAGAACGCCGCTCTGGATTTTGATGTGAAAACGCTG
GACTACGCCAGCAGCCCGATTGCCAATATCCGGGTCGGCAAACACACTGATAAAACCCGAGTGGTGCTGGACATGAACGA
GTCCGTGCGCACCCGTACTAACCTGCTGAAACCCATTGAGCCCCATGGCTGGCGCTTGGTGGTTGACCTGTTCGATAAAG
AGCTCACCACTGCGACCGCTAACGATAGTAAGCCAACGCCGGAAAAGTCGAAAAAGTCGGCTGAGCCCAAAGCCCAGCGA
TTAATGGTTGTGGCCATTGATGCCGGCCATGGTGGGGAAGACCCCGGTGCACGCGGGCCCAGTGGGGCCTATGAAAAAAC
CATTGTGCTGCAGATTGCCAAAAAGCTAGAGGCGATGGTGAATGAGAAAGCCGGCATGCGGGCGGTGATGGTGCGTGATG
GCGATTACTATGTGCCGCTGGTGGAGCGCCGCAAAATTGCCCGGGAAAAGCACGGGGCGGATGTGTTCGTTTCTATTCAC
GCGGATGCTTTCACCGATGCCCGCGCCAATGGCGCTTCGGTATTTGCCCTGTCCAACCGGGGGGCCACCAGTGCTCGGGC
CCGCTATCTGGCCAAAATAGCCAACGAGTCGGACCGCGTGGCCGGGGTGTATGAAGAAGAGAAAGACGACAGCTCGCTCT
ACAGTGTGCTGGCGGATCTGCAGATGAACGGTTCTATGGCCGGCAGTCTTTATCTTGGCCGGCAGGTGCTGTTGGAAATG
GGTAAAGTCACCAAGCTGCATGGTAACCGCGACAAGGTGGAGCAGGCAGGTTTTGCGGTGCTTAAAGAGCCGGAAATGGT
GTCGATCCTGGTGGAAACCGGGTTTATTTCCAACCCCACTGAAGAGCGTAATTTGCGCTCTTCAGCTCACCAGACCAAGC
TGGCTCGTTCAGTGGTCAAAGGGATTGATGCCTATTTTCGTAGCCATCCAGCACCTAATAGCTGGTATGCCGCTCAACGA
CGGGAGCAGGGCGATGAGTATCGAATTCAGCCCGGTGATACACTGTCGGAAATTGCTCGGCAGTACAGTGTTTCTGAGCA
GTCGATTCGTTCGGCTAACGGCATGGATGGCGATCGCATTATGGTCGGCCAGGTACTGAAAATTCCCCGTTCGTGA

Upstream 100 bases:

>100_bases
CGGAGCAGGCGTGGTGCCAGCACCGGATTTGACCATTACACTGGCCGTGAATGGCCACGGACGAAAAGCGACCCTGGAAT
GGGGTAGCGATTAAACGCCT

Downstream 100 bases:

>100_bases
CATTCCGCTTGCACGCAATACGCGCCATGCTGGCACGCTAATTTCCAAACGTGCTGGGTGCGGTGTGGCGGGTCAGATAA
TTTCCTAAAGGGTGTGTTTT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTMRKTVAVAFLSLLALCWSSLVFAQTTIDSVRLHRAPDHTRIVFDLPGPVDHKLDKLANPDRIVLDLQNAALDFDVKTL
DYASSPIANIRVGKHTDKTRVVLDMNESVRTRTNLLKPIEPHGWRLVVDLFDKELTTATANDSKPTPEKSKKSAEPKAQR
LMVVAIDAGHGGEDPGARGPSGAYEKTIVLQIAKKLEAMVNEKAGMRAVMVRDGDYYVPLVERRKIAREKHGADVFVSIH
ADAFTDARANGASVFALSNRGATSARARYLAKIANESDRVAGVYEEEKDDSSLYSVLADLQMNGSMAGSLYLGRQVLLEM
GKVTKLHGNRDKVEQAGFAVLKEPEMVSILVETGFISNPTEERNLRSSAHQTKLARSVVKGIDAYFRSHPAPNSWYAAQR
REQGDEYRIQPGDTLSEIARQYSVSEQSIRSANGMDGDRIMVGQVLKIPRS

Sequences:

>Translated_451_residues
MTMRKTVAVAFLSLLALCWSSLVFAQTTIDSVRLHRAPDHTRIVFDLPGPVDHKLDKLANPDRIVLDLQNAALDFDVKTL
DYASSPIANIRVGKHTDKTRVVLDMNESVRTRTNLLKPIEPHGWRLVVDLFDKELTTATANDSKPTPEKSKKSAEPKAQR
LMVVAIDAGHGGEDPGARGPSGAYEKTIVLQIAKKLEAMVNEKAGMRAVMVRDGDYYVPLVERRKIAREKHGADVFVSIH
ADAFTDARANGASVFALSNRGATSARARYLAKIANESDRVAGVYEEEKDDSSLYSVLADLQMNGSMAGSLYLGRQVLLEM
GKVTKLHGNRDKVEQAGFAVLKEPEMVSILVETGFISNPTEERNLRSSAHQTKLARSVVKGIDAYFRSHPAPNSWYAAQR
REQGDEYRIQPGDTLSEIARQYSVSEQSIRSANGMDGDRIMVGQVLKIPRS
>Mature_450_residues
TMRKTVAVAFLSLLALCWSSLVFAQTTIDSVRLHRAPDHTRIVFDLPGPVDHKLDKLANPDRIVLDLQNAALDFDVKTLD
YASSPIANIRVGKHTDKTRVVLDMNESVRTRTNLLKPIEPHGWRLVVDLFDKELTTATANDSKPTPEKSKKSAEPKAQRL
MVVAIDAGHGGEDPGARGPSGAYEKTIVLQIAKKLEAMVNEKAGMRAVMVRDGDYYVPLVERRKIAREKHGADVFVSIHA
DAFTDARANGASVFALSNRGATSARARYLAKIANESDRVAGVYEEEKDDSSLYSVLADLQMNGSMAGSLYLGRQVLLEMG
KVTKLHGNRDKVEQAGFAVLKEPEMVSILVETGFISNPTEERNLRSSAHQTKLARSVVKGIDAYFRSHPAPNSWYAAQRR
EQGDEYRIQPGDTLSEIARQYSVSEQSIRSANGMDGDRIMVGQVLKIPRS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=392, Percent_Identity=38.0102040816327, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788776, Length=267, Percent_Identity=44.5692883895131, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1790611, Length=232, Percent_Identity=40.0862068965517, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 49768; Mature: 49637

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMRKTVAVAFLSLLALCWSSLVFAQTTIDSVRLHRAPDHTRIVFDLPGPVDHKLDKLAN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCHHHHHHCCC
PDRIVLDLQNAALDFDVKTLDYASSPIANIRVGKHTDKTRVVLDMNESVRTRTNLLKPIE
CCEEEEEECCCCEEECEEEHHCCCCCCCEEEECCCCCCEEEEEECCCHHHHHHHHCCCCC
PHGWRLVVDLFDKELTTATANDSKPTPEKSKKSAEPKAQRLMVVAIDAGHGGEDPGARGP
CCCCEEEEEECCCHHHEECCCCCCCCCCHHHCCCCCHHHEEEEEEEECCCCCCCCCCCCC
SGAYEKTIVLQIAKKLEAMVNEKAGMRAVMVRDGDYYVPLVERRKIAREKHGADVFVSIH
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECHHHHHHHHHHHCCCEEEEEEE
ADAFTDARANGASVFALSNRGATSARARYLAKIANESDRVAGVYEEEKDDSSLYSVLADL
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHH
QMNGSMAGSLYLGRQVLLEMGKVTKLHGNRDKVEQAGFAVLKEPEMVSILVETGFISNPT
HCCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHCCHHEECCCCEEEEEEECCCCCCCC
EERNLRSSAHQTKLARSVVKGIDAYFRSHPAPNSWYAAQRREQGDEYRIQPGDTLSEIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEECCCCHHHHHHH
QYSVSEQSIRSANGMDGDRIMVGQVLKIPRS
HHCCCHHHHHHCCCCCCCEEEEHHHHCCCCC
>Mature Secondary Structure 
TMRKTVAVAFLSLLALCWSSLVFAQTTIDSVRLHRAPDHTRIVFDLPGPVDHKLDKLAN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCHHHHHHCCC
PDRIVLDLQNAALDFDVKTLDYASSPIANIRVGKHTDKTRVVLDMNESVRTRTNLLKPIE
CCEEEEEECCCCEEECEEEHHCCCCCCCEEEECCCCCCEEEEEECCCHHHHHHHHCCCCC
PHGWRLVVDLFDKELTTATANDSKPTPEKSKKSAEPKAQRLMVVAIDAGHGGEDPGARGP
CCCCEEEEEECCCHHHEECCCCCCCCCCHHHCCCCCHHHEEEEEEEECCCCCCCCCCCCC
SGAYEKTIVLQIAKKLEAMVNEKAGMRAVMVRDGDYYVPLVERRKIAREKHGADVFVSIH
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECHHHHHHHHHHHCCCEEEEEEE
ADAFTDARANGASVFALSNRGATSARARYLAKIANESDRVAGVYEEEKDDSSLYSVLADL
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHH
QMNGSMAGSLYLGRQVLLEMGKVTKLHGNRDKVEQAGFAVLKEPEMVSILVETGFISNPT
HCCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHCCHHEECCCCEEEEEEECCCCCCCC
EERNLRSSAHQTKLARSVVKGIDAYFRSHPAPNSWYAAQRREQGDEYRIQPGDTLSEIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEECCCCHHHHHHH
QYSVSEQSIRSANGMDGDRIMVGQVLKIPRS
HHCCCHHHHHHCCCCCCCEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]