Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 110834877

GI number: 110834877

Start: 2302686

End: 2303984

Strand: Direct

Name: purD [H]

Synonym: ABO_2016

Alternate gene names: 110834877

Gene position: 2302686-2303984 (Clockwise)

Preceding gene: 110834876

Following gene: 110834878

Centisome position: 73.8

GC content: 60.35

Gene sequence:

>1299_bases
ATGGATCACCCCATGAAGGTACTCATCATTGGCGGCGGTGGCCGCGAGCATGCCCTGGCATGGAAAGTGGCTCAAGCCGA
GCAGGTAGAAACCGTCTTCGTTGCCCCCGGCAACGCGGGTACCGCCCGCGAAGCCAAGCTCGAAAATGTGGCCCTGGACG
TACTGGATCAACCCGGGCTGCTGGCCTTTGCCAAAGACAATAGCATCGACCTGACCATTGTTGGCCCGGAAGCGCCACTG
GTAGAGGGTTTGGTCGACACCTTCCAGGAAAATGGCCTGAAGTGCTTCGGTCCGAACAAGGCTGCAGCACAACTGGAAGG
CTCCAAAGCCTTCACCAAAGACTTTCTGGCCCGGCAGAACATTCCCACCGGCGCCTACGGCAACTTCACTGACATGGATG
AAGCGCTGGCCTACGTGCGCGAACAGGGCGCTCCTATCGTGATCAAGGCCGATGGGCTGGCCGCAGGCAAAGGTGTGATC
GTCGCCATGACTCTGCAGGAAGCGGAAGATGCCGTACGCGACATGCTTGACGGCAACAAATTTGGCGATGCCGGCCACCG
TGTAGTGGTCGAAGAGTTTCTCGAAGGCGAAGAGGCCAGCTTCATCGTCATGGTCGATGGCAAAAACGTTCTGCCCATGG
CTACCAGCCAGGATCACAAGCGAGCCGGCGACGGCGACACGGGCCCCAACACCGGCGGCATGGGCGCTTACTCCCCGGCG
CCGGTGGTCACCGACGCGGTCTACAAACGCATCATGGACGAAGTCATCACCCCCACCGTAGAAGGCATGGCCGCCGAAGG
CATGCCCTACACCGGCTTCCTCTACGCGGGCTTGATGATCAACCCAGACGGTGCCCCCAAAGTAATCGAATACAATTGCC
GCTTCGGCGACCCGGAAACTCAGCCCATCATGATGCGCCTGCAGTCCGACCTGGCTGCGCTATGTCTGGCCGCGCTGGAA
GGTAAGCTGGATCAGGCCGAGATTCAATGGGATCCACGTCCCACGCTAGGTGTGGTAATGGCCGCTGGCGGCTACCCGGA
TAGCTACGACAAGGGGGATGCCATCAAGGGGCTGGATCAGGCCGATTCTGATACTGTGAAGGTCTTCCACGCCGGGACCG
CCGAACAAGACGGCCAGGTGGTTACCAGTGGCGGGCGGGTGCTCTGCATCACCGGTGTCGGCGACACCGTCGGTGAAGCC
CAGGCCAATGCCTACGAGGGTGTGAAGCAGATCACATGGCGTAATGCCCAGTATCGTTCCGACATTGGCTACCGTGCAGT
GGCCCGCGAAAAAGCATAA

Upstream 100 bases:

>100_bases
CGTCCAACGCATGAAGCGACCCTCCGGTCCCGCGTTGGACCTCTGGCGTCAAGCTCGTCGCCGATCACGACACGCGATCA
GGATTAACCATTCATAGCAA

Downstream 100 bases:

>100_bases
AGTACAGGCAACTAAGAATTAAAGGTGAGCCGGCTCCGGCTCACCCATCAACACACCTTCGGGGGTTGCCATGTCGAATC
AAACGGCGGATATGTCACGT

Product: phosphoribosylamine-glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MDHPMKVLIIGGGGREHALAWKVAQAEQVETVFVAPGNAGTAREAKLENVALDVLDQPGLLAFAKDNSIDLTIVGPEAPL
VEGLVDTFQENGLKCFGPNKAAAQLEGSKAFTKDFLARQNIPTGAYGNFTDMDEALAYVREQGAPIVIKADGLAAGKGVI
VAMTLQEAEDAVRDMLDGNKFGDAGHRVVVEEFLEGEEASFIVMVDGKNVLPMATSQDHKRAGDGDTGPNTGGMGAYSPA
PVVTDAVYKRIMDEVITPTVEGMAAEGMPYTGFLYAGLMINPDGAPKVIEYNCRFGDPETQPIMMRLQSDLAALCLAALE
GKLDQAEIQWDPRPTLGVVMAAGGYPDSYDKGDAIKGLDQADSDTVKVFHAGTAEQDGQVVTSGGRVLCITGVGDTVGEA
QANAYEGVKQITWRNAQYRSDIGYRAVAREKA

Sequences:

>Translated_432_residues
MDHPMKVLIIGGGGREHALAWKVAQAEQVETVFVAPGNAGTAREAKLENVALDVLDQPGLLAFAKDNSIDLTIVGPEAPL
VEGLVDTFQENGLKCFGPNKAAAQLEGSKAFTKDFLARQNIPTGAYGNFTDMDEALAYVREQGAPIVIKADGLAAGKGVI
VAMTLQEAEDAVRDMLDGNKFGDAGHRVVVEEFLEGEEASFIVMVDGKNVLPMATSQDHKRAGDGDTGPNTGGMGAYSPA
PVVTDAVYKRIMDEVITPTVEGMAAEGMPYTGFLYAGLMINPDGAPKVIEYNCRFGDPETQPIMMRLQSDLAALCLAALE
GKLDQAEIQWDPRPTLGVVMAAGGYPDSYDKGDAIKGLDQADSDTVKVFHAGTAEQDGQVVTSGGRVLCITGVGDTVGEA
QANAYEGVKQITWRNAQYRSDIGYRAVAREKA
>Mature_432_residues
MDHPMKVLIIGGGGREHALAWKVAQAEQVETVFVAPGNAGTAREAKLENVALDVLDQPGLLAFAKDNSIDLTIVGPEAPL
VEGLVDTFQENGLKCFGPNKAAAQLEGSKAFTKDFLARQNIPTGAYGNFTDMDEALAYVREQGAPIVIKADGLAAGKGVI
VAMTLQEAEDAVRDMLDGNKFGDAGHRVVVEEFLEGEEASFIVMVDGKNVLPMATSQDHKRAGDGDTGPNTGGMGAYSPA
PVVTDAVYKRIMDEVITPTVEGMAAEGMPYTGFLYAGLMINPDGAPKVIEYNCRFGDPETQPIMMRLQSDLAALCLAALE
GKLDQAEIQWDPRPTLGVVMAAGGYPDSYDKGDAIKGLDQADSDTVKVFHAGTAEQDGQVVTSGGRVLCITGVGDTVGEA
QANAYEGVKQITWRNAQYRSDIGYRAVAREKA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=49.1764705882353, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=49.1764705882353, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=49.1764705882353, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=49.1764705882353, Blast_Score=413, Evalue=1e-115,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=70.5607476635514, Blast_Score=606, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=41.7840375586854, Blast_Score=314, Evalue=5e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=448, Percent_Identity=42.8571428571429, Blast_Score=354, Evalue=1e-98,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=45.1612903225806, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=45.1612903225806, Blast_Score=342, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45775; Mature: 45775

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHPMKVLIIGGGGREHALAWKVAQAEQVETVFVAPGNAGTAREAKLENVALDVLDQPGL
CCCCEEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCE
LAFAKDNSIDLTIVGPEAPLVEGLVDTFQENGLKCFGPNKAAAQLEGSKAFTKDFLARQN
EEEECCCCEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCHHHEECCCHHHHHHHHHHCC
IPTGAYGNFTDMDEALAYVREQGAPIVIKADGLAAGKGVIVAMTLQEAEDAVRDMLDGNK
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCC
FGDAGHRVVVEEFLEGEEASFIVMVDGKNVLPMATSQDHKRAGDGDTGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTDAVYKRIMDEVITPTVEGMAAEGMPYTGFLYAGLMINPDGAPKVIEYNCRFGDPET
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCC
QPIMMRLQSDLAALCLAALEGKLDQAEIQWDPRPTLGVVMAAGGYPDSYDKGDAIKGLDQ
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCHHCCCCC
ADSDTVKVFHAGTAEQDGQVVTSGGRVLCITGVGDTVGEAQANAYEGVKQITWRNAQYRS
CCCCEEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHC
DIGYRAVAREKA
CCCHHHHHCCCC
>Mature Secondary Structure
MDHPMKVLIIGGGGREHALAWKVAQAEQVETVFVAPGNAGTAREAKLENVALDVLDQPGL
CCCCEEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCE
LAFAKDNSIDLTIVGPEAPLVEGLVDTFQENGLKCFGPNKAAAQLEGSKAFTKDFLARQN
EEEECCCCEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCHHHEECCCHHHHHHHHHHCC
IPTGAYGNFTDMDEALAYVREQGAPIVIKADGLAAGKGVIVAMTLQEAEDAVRDMLDGNK
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCC
FGDAGHRVVVEEFLEGEEASFIVMVDGKNVLPMATSQDHKRAGDGDTGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTDAVYKRIMDEVITPTVEGMAAEGMPYTGFLYAGLMINPDGAPKVIEYNCRFGDPET
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCC
QPIMMRLQSDLAALCLAALEGKLDQAEIQWDPRPTLGVVMAAGGYPDSYDKGDAIKGLDQ
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCCCCCHHCCCCC
ADSDTVKVFHAGTAEQDGQVVTSGGRVLCITGVGDTVGEAQANAYEGVKQITWRNAQYRS
CCCCEEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHC
DIGYRAVAREKA
CCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]