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The map label for this gene is pleD [H]

Identifier: 110834172

GI number: 110834172

Start: 1488119

End: 1489255

Strand: Direct

Name: pleD [H]

Synonym: ABO_1311

Alternate gene names: 110834172

Gene position: 1488119-1489255 (Clockwise)

Preceding gene: 110834171

Following gene: 110834173

Centisome position: 47.69

GC content: 49.08

Gene sequence:

>1137_bases
ATGAAACAAAACATTTTGGTTGTGGATGATCACCGCGAAAACTTGATCGCCCTGGAGGCGATCCTGGATTCTGACGACCG
TAATCTGATCATGGCACAGTCCGGTAACGAGGCCTTGTCCCTCGCCCTCAAGCACGATTTAGCTCTGGTGCTGTTGGATG
TGCAGATGCCGGAAATGGATGGGTTCGAAGTGGCCGAACTTATGCGTAAAAATAAGAAAACCCGCCACATTCCGATCATT
TTTGTTACCGCTATATCAAAAGAACAGAAATACGTTTTCAAGGGCTACGAGTGTGGGGCGGTGGATTACCTACTCAAACC
CATCGACCAAAAAATTCTGGAAGCCAAGGTTAGCGTTTTCCTGGAACTGGATCGCCAACGGCGCAAGTTACAACAGGCCG
TGGTGCAAATGAAACGGCTAAAAGACGAAAACGAGCGACTGCTGCATGCACTTGGCGAAGCTCTGATCGGCACCAATGCC
GAGGGTAAAATCACTTTTTGTAATGAAGCAGCTTGTGCCCTATTGCGACAGAGTCGTGATGACTTAGTAGGCAATGATAT
CGCCGACCTGATATTCAATGATGATGCTGGCAAGTCGTGTTGGAGCTGGGATAGCTCTCCATTACAGGAAGCTTGCAGAC
AAGGGGAAAGCTGGGAAAATGAGCATAACCTGTATACTCCCGATGATGATGGCTTGCTAGCCTTATCAGTGAGAGCCAAT
GCGATTACTAATGATGCAGGCATCTTCACAGGGGTGGTCGTGGTACTTGGCCTCTCAGATGATGAAAATAATGGCGGAGA
CTGCGCTCGTAACCATCGCCGTAACCCTCGCAAGAGGTTGTTCCGCGAAATGGTAGTGTTTGACCGTAGCACCGGTGGTA
ATGTGGGGCGCTTAGTAAACTTGAGTGTGAACGGCTTCAAGCTTTTTTCCCGCCGAGCATTAGCCCATGGTGAGGATTTG
CAACTTGGCATGGTGCTGCCCGACCAGATTTCAGGGGTAAACACCGTCAGCTTTGATGCCCGGGTTGTTTGGGCGGAAAC
GTTAGATGCCCCTGGTGAGTTTCAGGTAGGTTTTCAGTTTCAGACGTTGAATTCGGTCACCCGTGATGTGATCGAAAACC
TAATGGAAAACTATTAA

Upstream 100 bases:

>100_bases
ATGCCTGAAGCGGCAATAACAACAGGTGCGATTTCCAGGGTACTCGATCTTGAGGCAATCGGGCCCTTTTTGGCTAAACA
GGCTATGGGGTAGGACGCGT

Downstream 100 bases:

>100_bases
ATTATGGCTGAGCACATGGAGTCGCCTGAGAACATAGAGCTTTCTTCATCGTTGTTGCCCTATGGTTTCGCGCGGCGTTT
CGGTGTTCTGTTGCGTGAGC

Product: sensory box protein/response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKQNILVVDDHRENLIALEAILDSDDRNLIMAQSGNEALSLALKHDLALVLLDVQMPEMDGFEVAELMRKNKKTRHIPII
FVTAISKEQKYVFKGYECGAVDYLLKPIDQKILEAKVSVFLELDRQRRKLQQAVVQMKRLKDENERLLHALGEALIGTNA
EGKITFCNEAACALLRQSRDDLVGNDIADLIFNDDAGKSCWSWDSSPLQEACRQGESWENEHNLYTPDDDGLLALSVRAN
AITNDAGIFTGVVVVLGLSDDENNGGDCARNHRRNPRKRLFREMVVFDRSTGGNVGRLVNLSVNGFKLFSRRALAHGEDL
QLGMVLPDQISGVNTVSFDARVVWAETLDAPGEFQVGFQFQTLNSVTRDVIENLMENY

Sequences:

>Translated_378_residues
MKQNILVVDDHRENLIALEAILDSDDRNLIMAQSGNEALSLALKHDLALVLLDVQMPEMDGFEVAELMRKNKKTRHIPII
FVTAISKEQKYVFKGYECGAVDYLLKPIDQKILEAKVSVFLELDRQRRKLQQAVVQMKRLKDENERLLHALGEALIGTNA
EGKITFCNEAACALLRQSRDDLVGNDIADLIFNDDAGKSCWSWDSSPLQEACRQGESWENEHNLYTPDDDGLLALSVRAN
AITNDAGIFTGVVVVLGLSDDENNGGDCARNHRRNPRKRLFREMVVFDRSTGGNVGRLVNLSVNGFKLFSRRALAHGEDL
QLGMVLPDQISGVNTVSFDARVVWAETLDAPGEFQVGFQFQTLNSVTRDVIENLMENY
>Mature_378_residues
MKQNILVVDDHRENLIALEAILDSDDRNLIMAQSGNEALSLALKHDLALVLLDVQMPEMDGFEVAELMRKNKKTRHIPII
FVTAISKEQKYVFKGYECGAVDYLLKPIDQKILEAKVSVFLELDRQRRKLQQAVVQMKRLKDENERLLHALGEALIGTNA
EGKITFCNEAACALLRQSRDDLVGNDIADLIFNDDAGKSCWSWDSSPLQEACRQGESWENEHNLYTPDDDGLLALSVRAN
AITNDAGIFTGVVVVLGLSDDENNGGDCARNHRRNPRKRLFREMVVFDRSTGGNVGRLVNLSVNGFKLFSRRALAHGEDL
QLGMVLPDQISGVNTVSFDARVVWAETLDAPGEFQVGFQFQTLNSVTRDVIENLMENY

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=37.719298245614, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789149, Length=115, Percent_Identity=33.0434782608696, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42348; Mature: 42348

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQNILVVDDHRENLIALEAILDSDDRNLIMAQSGNEALSLALKHDLALVLLDVQMPEMD
CCCCEEEEECCCCCEEEEEEHHCCCCCCEEEEECCCHHEEEHHHCCEEEEEEEEECCCCC
GFEVAELMRKNKKTRHIPIIFVTAISKEQKYVFKGYECGAVDYLLKPIDQKILEAKVSVF
CHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LELDRQRRKLQQAVVQMKRLKDENERLLHALGEALIGTNAEGKITFCNEAACALLRQSRD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHCCH
DLVGNDIADLIFNDDAGKSCWSWDSSPLQEACRQGESWENEHNLYTPDDDGLLALSVRAN
HHCCCCHHHHEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECC
AITNDAGIFTGVVVVLGLSDDENNGGDCARNHRRNPRKRLFREMVVFDRSTGGNVGRLVN
CCCCCCHHHEEEEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
LSVNGFKLFSRRALAHGEDLQLGMVLPDQISGVNTVSFDARVVWAETLDAPGEFQVGFQF
EECCHHHHHHHHHHCCCCCCEEEEECCHHCCCCEEEECCEEEEEEECCCCCCCEEEEEEE
QTLNSVTRDVIENLMENY
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQNILVVDDHRENLIALEAILDSDDRNLIMAQSGNEALSLALKHDLALVLLDVQMPEMD
CCCCEEEEECCCCCEEEEEEHHCCCCCCEEEEECCCHHEEEHHHCCEEEEEEEEECCCCC
GFEVAELMRKNKKTRHIPIIFVTAISKEQKYVFKGYECGAVDYLLKPIDQKILEAKVSVF
CHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LELDRQRRKLQQAVVQMKRLKDENERLLHALGEALIGTNAEGKITFCNEAACALLRQSRD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHCCH
DLVGNDIADLIFNDDAGKSCWSWDSSPLQEACRQGESWENEHNLYTPDDDGLLALSVRAN
HHCCCCHHHHEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECC
AITNDAGIFTGVVVVLGLSDDENNGGDCARNHRRNPRKRLFREMVVFDRSTGGNVGRLVN
CCCCCCHHHEEEEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
LSVNGFKLFSRRALAHGEDLQLGMVLPDQISGVNTVSFDARVVWAETLDAPGEFQVGFQF
EECCHHHHHHHHHHCCCCCCEEEEECCHHCCCCEEEECCEEEEEEECCCCCCCEEEEEEE
QTLNSVTRDVIENLMENY
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA