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The map label for this gene is gspE [H]

Identifier: 110834173

GI number: 110834173

Start: 1489259

End: 1490761

Strand: Direct

Name: gspE [H]

Synonym: ABO_1312

Alternate gene names: 110834173

Gene position: 1489259-1490761 (Clockwise)

Preceding gene: 110834172

Following gene: 110834174

Centisome position: 47.73

GC content: 55.42

Gene sequence:

>1503_bases
ATGGCTGAGCACATGGAGTCGCCTGAGAACATAGAGCTTTCTTCATCGTTGTTGCCCTATGGTTTCGCGCGGCGTTTCGG
TGTTCTGTTGCGTGAGCGCGATGGGCAGAAAGAGTTGTGTCACCGGCAGGATGTTTCACTGGCCGCGCTAGGTGAGGTGC
AACGTCATATCGGCCGCCTGCCGCGCCTAGTACCCATGGACGACACGGCTTTTAGCGCCGCCATGGCAATCACCTATGAA
CAGCAGCGCGGCGCGGCAGCAGAAGCCGCCGATGATCTGGAAGGACTTGATCTGGCAAGCCTAGCAGACTCACTGCCGGA
AACGTCGGATTTGCTTGAGCAGGAAGACGATGCTCCGATCATTCGGCTGATCAATGCCTTGTTACAGGAAGCCATCCGTG
AAGATGCCTCCGATATCCACATCGAAACCTTCGAACGACGGCTAGTAGTTCGTATGCGAGTCGATGGTGTCCTTCGTGAA
GTGCTTACCCCGAAAAGGGAACTGGCGCCGTTGTTAGTCTCGCGAATCAAGGTAATGGCGCGAATGGATATCGCTGAAAA
GCGGATACCTCAGGATGGTCGAATCTCCTTGCGCATTGGTGGGCGCGATGTGGATGTGCGGGTATCGACTATGCCCTCCA
GTAATGGTGAGCGGGTGGTGCTACGCTTGTTGGACAAGCAAGCGGGGCGTTTACAGGTATCGCATTTGGGCATGGGCGAA
ACCTGTGACGCTCATATGCGCGAGCTAATCCATAAACCCCATGGCATTATTCTGGTGACCGGCCCGACAGGGTCCGGTAA
AACCACAACGCTTTATGCTTCCCTAACCGAATTGAATGACAGTACCCGCAATATTCTTACGGTAGAAGATCCCATCGAAT
ACCAGCTAGAAGGGATCGGCCAGACTCAAGTCAACACTAAAGTCGACATGACATTTGCCCGCGGACTGCGTGCCATCCTT
CGCCAAGATCCAGATGTGGTGATGGTGGGGGAGATCCGGGATCTGGAAACCGCTGAAATTGCAGTGCAAGCCTCCCTGAC
CGGCCACCTTGTACTGTCTACTTTGCATACCAATACAGCAGTGGGGGCGGTGACCCGCTTGCAGGACATGGGCATTGAAC
CTTTCTTGCTCTCTAGCTCCCTGCTGGGTTCCTTGGCTCAGCGGCTGGTGCGTGTGCTATGCGATGACTGTAAAACCCCG
TACCAGGCTAGCGCTAGTGAGCTGGAAATGATGGGGTTGGCGGCTACGGAGAGCCGTACGCTGTATCACCCCAATGGTTG
TGAATCCTGTAACCATCAGGGTTACCGGGGCCGTACCGGGATCTATGAACTGGTACTGGTGGATGACACGCTACGCCAGT
TGATCCACGATCGCGCCGGCGAGCTAGAACTTAACCGCCATGCTCGGACACTAACTAGCGGTATCCGCGAAGATGGTTGG
CGAAAAGTGCTCGGCGGACACACCAGTGTAGAAGAGGTCCTGCGGGTAACCCGCGAGGACTAA

Upstream 100 bases:

>100_bases
GTTAGATGCCCCTGGTGAGTTTCAGGTAGGTTTTCAGTTTCAGACGTTGAATTCGGTCACCCGTGATGTGATCGAAAACC
TAATGGAAAACTATTAAATT

Downstream 100 bases:

>100_bases
CGGGACAGTATTCTCCATGCCAGCATTTGAATACCGCTCTCTGGATCACAGGGGCAAGATCAACAAAGGCACGCTGGAAG
CAGACAGTGCCCGCCAGGTG

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAEHMESPENIELSSSLLPYGFARRFGVLLRERDGQKELCHRQDVSLAALGEVQRHIGRLPRLVPMDDTAFSAAMAITYE
QQRGAAAEAADDLEGLDLASLADSLPETSDLLEQEDDAPIIRLINALLQEAIREDASDIHIETFERRLVVRMRVDGVLRE
VLTPKRELAPLLVSRIKVMARMDIAEKRIPQDGRISLRIGGRDVDVRVSTMPSSNGERVVLRLLDKQAGRLQVSHLGMGE
TCDAHMRELIHKPHGIILVTGPTGSGKTTTLYASLTELNDSTRNILTVEDPIEYQLEGIGQTQVNTKVDMTFARGLRAIL
RQDPDVVMVGEIRDLETAEIAVQASLTGHLVLSTLHTNTAVGAVTRLQDMGIEPFLLSSSLLGSLAQRLVRVLCDDCKTP
YQASASELEMMGLAATESRTLYHPNGCESCNHQGYRGRTGIYELVLVDDTLRQLIHDRAGELELNRHARTLTSGIREDGW
RKVLGGHTSVEEVLRVTRED

Sequences:

>Translated_500_residues
MAEHMESPENIELSSSLLPYGFARRFGVLLRERDGQKELCHRQDVSLAALGEVQRHIGRLPRLVPMDDTAFSAAMAITYE
QQRGAAAEAADDLEGLDLASLADSLPETSDLLEQEDDAPIIRLINALLQEAIREDASDIHIETFERRLVVRMRVDGVLRE
VLTPKRELAPLLVSRIKVMARMDIAEKRIPQDGRISLRIGGRDVDVRVSTMPSSNGERVVLRLLDKQAGRLQVSHLGMGE
TCDAHMRELIHKPHGIILVTGPTGSGKTTTLYASLTELNDSTRNILTVEDPIEYQLEGIGQTQVNTKVDMTFARGLRAIL
RQDPDVVMVGEIRDLETAEIAVQASLTGHLVLSTLHTNTAVGAVTRLQDMGIEPFLLSSSLLGSLAQRLVRVLCDDCKTP
YQASASELEMMGLAATESRTLYHPNGCESCNHQGYRGRTGIYELVLVDDTLRQLIHDRAGELELNRHARTLTSGIREDGW
RKVLGGHTSVEEVLRVTRED
>Mature_499_residues
AEHMESPENIELSSSLLPYGFARRFGVLLRERDGQKELCHRQDVSLAALGEVQRHIGRLPRLVPMDDTAFSAAMAITYEQ
QRGAAAEAADDLEGLDLASLADSLPETSDLLEQEDDAPIIRLINALLQEAIREDASDIHIETFERRLVVRMRVDGVLREV
LTPKRELAPLLVSRIKVMARMDIAEKRIPQDGRISLRIGGRDVDVRVSTMPSSNGERVVLRLLDKQAGRLQVSHLGMGET
CDAHMRELIHKPHGIILVTGPTGSGKTTTLYASLTELNDSTRNILTVEDPIEYQLEGIGQTQVNTKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAEIAVQASLTGHLVLSTLHTNTAVGAVTRLQDMGIEPFLLSSSLLGSLAQRLVRVLCDDCKTPY
QASASELEMMGLAATESRTLYHPNGCESCNHQGYRGRTGIYELVLVDDTLRQLIHDRAGELELNRHARTLTSGIREDGWR
KVLGGHTSVEEVLRVTRED

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=478, Percent_Identity=55.2301255230126, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1786296, Length=386, Percent_Identity=42.7461139896373, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI87082188, Length=248, Percent_Identity=36.2903225806452, Blast_Score=118, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55362; Mature: 55231

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHMESPENIELSSSLLPYGFARRFGVLLRERDGQKELCHRQDVSLAALGEVQRHIGRL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCC
PRLVPMDDTAFSAAMAITYEQQRGAAAEAADDLEGLDLASLADSLPETSDLLEQEDDAPI
CCCCCCCCCHHHHHEEEEEHHHCCCHHHHHHCCCCCCHHHHHHCCCCHHHHHHCCCCCHH
IRLINALLQEAIREDASDIHIETFERRLVVRMRVDGVLREVLTPKRELAPLLVSRIKVMA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RMDIAEKRIPQDGRISLRIGGRDVDVRVSTMPSSNGERVVLRLLDKQAGRLQVSHLGMGE
HHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCC
TCDAHMRELIHKPHGIILVTGPTGSGKTTTLYASLTELNDSTRNILTVEDPIEYQLEGIG
CHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEHHHCCCCCCCEEEECCCCHHHHCCCC
QTQVNTKVDMTFARGLRAILRQDPDVVMVGEIRDLETAEIAVQASLTGHLVLSTLHTNTA
CCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCEEEEEEHHCCHH
VGAVTRLQDMGIEPFLLSSSLLGSLAQRLVRVLCDDCKTPYQASASELEMMGLAATESRT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEEECCCCE
LYHPNGCESCNHQGYRGRTGIYELVLVDDTLRQLIHDRAGELELNRHARTLTSGIREDGW
EECCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHH
RKVLGGHTSVEEVLRVTRED
HHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
AEHMESPENIELSSSLLPYGFARRFGVLLRERDGQKELCHRQDVSLAALGEVQRHIGRL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCC
PRLVPMDDTAFSAAMAITYEQQRGAAAEAADDLEGLDLASLADSLPETSDLLEQEDDAPI
CCCCCCCCCHHHHHEEEEEHHHCCCHHHHHHCCCCCCHHHHHHCCCCHHHHHHCCCCCHH
IRLINALLQEAIREDASDIHIETFERRLVVRMRVDGVLREVLTPKRELAPLLVSRIKVMA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RMDIAEKRIPQDGRISLRIGGRDVDVRVSTMPSSNGERVVLRLLDKQAGRLQVSHLGMGE
HHHHHHHHCCCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCC
TCDAHMRELIHKPHGIILVTGPTGSGKTTTLYASLTELNDSTRNILTVEDPIEYQLEGIG
CHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEHHHCCCCCCCEEEECCCCHHHHCCCC
QTQVNTKVDMTFARGLRAILRQDPDVVMVGEIRDLETAEIAVQASLTGHLVLSTLHTNTA
CCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCEEEEEEHHCCHH
VGAVTRLQDMGIEPFLLSSSLLGSLAQRLVRVLCDDCKTPYQASASELEMMGLAATESRT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEEECCCCE
LYHPNGCESCNHQGYRGRTGIYELVLVDDTLRQLIHDRAGELELNRHARTLTSGIREDGW
EECCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHH
RKVLGGHTSVEEVLRVTRED
HHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]