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The map label for this gene is gloA [H]

Identifier: 110833587

GI number: 110833587

Start: 817224

End: 817607

Strand: Direct

Name: gloA [H]

Synonym: ABO_0726

Alternate gene names: 110833587

Gene position: 817224-817607 (Clockwise)

Preceding gene: 110833586

Following gene: 110833588

Centisome position: 26.19

GC content: 55.21

Gene sequence:

>384_bases
ATGCGAATCCTGCATACCATGTTGCGTGTGGGCGACCTTGAGGCGTCCGTTGCTTTCTATACTCAGGTGCTGGGAATGAC
CGAATTGCGTCGTAAAGAGTATCCGGAAGGCCGCTTTACCAATGTTTTTGTGGGCTTCCAGCCAGAATCTGAGGGTGCGG
TGCTGGAGTTGACCTACAACTGGGATCAGAGCGCCTATGATCTGGGCGACGGTTATGGTCATGTGGCTCTTGCCGTGGAC
GACGTGTACGCTGCTTGTGAGCGTATTCGGGAAAAAGGTGGCCGTATTACCCGTGAGCCGGGGCCCATGAAACACGGCAC
TACGGTACTGGCCTTTGCCCAAGACCCGGATGGCTACAAAGTCGAATTGCTGGGACGCGATTGA

Upstream 100 bases:

>100_bases
TTTCTTGTTAATTCACCAAACTGACACCACACTCCGCCCTGATAGGGCAGGGCCTGTACAAGCCTTGTAGCGTTCTAATA
TCGTTGGGAGCCATTCTTGC

Downstream 100 bases:

>100_bases
TGGTATCGAATGATTCGCAGGCACTTAGCAAGGTGACGGTAGGGTGGCGCGAGTGGGCTACTTTGCCGGAGCTGGGTATT
GATGCCATCAAGATGAAGGT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MRILHTMLRVGDLEASVAFYTQVLGMTELRRKEYPEGRFTNVFVGFQPESEGAVLELTYNWDQSAYDLGDGYGHVALAVD
DVYAACERIREKGGRITREPGPMKHGTTVLAFAQDPDGYKVELLGRD

Sequences:

>Translated_127_residues
MRILHTMLRVGDLEASVAFYTQVLGMTELRRKEYPEGRFTNVFVGFQPESEGAVLELTYNWDQSAYDLGDGYGHVALAVD
DVYAACERIREKGGRITREPGPMKHGTTVLAFAQDPDGYKVELLGRD
>Mature_127_residues
MRILHTMLRVGDLEASVAFYTQVLGMTELRRKEYPEGRFTNVFVGFQPESEGAVLELTYNWDQSAYDLGDGYGHVALAVD
DVYAACERIREKGGRITREPGPMKHGTTVLAFAQDPDGYKVELLGRD

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=147, Percent_Identity=38.0952380952381, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1787940, Length=124, Percent_Identity=61.2903225806452, Blast_Score=169, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=35.2517985611511, Blast_Score=82, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=38.5135135135135, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19920404, Length=130, Percent_Identity=34.6153846153846, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14246; Mature: 14246

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTMLRVGDLEASVAFYTQVLGMTELRRKEYPEGRFTNVFVGFQPESEGAVLELTYN
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEEC
WDQSAYDLGDGYGHVALAVDDVYAACERIREKGGRITREPGPMKHGTTVLAFAQDPDGYK
CCCCCEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCE
VELLGRD
EEEEECC
>Mature Secondary Structure
MRILHTMLRVGDLEASVAFYTQVLGMTELRRKEYPEGRFTNVFVGFQPESEGAVLELTYN
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEEC
WDQSAYDLGDGYGHVALAVDDVYAACERIREKGGRITREPGPMKHGTTVLAFAQDPDGYK
CCCCCEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCE
VELLGRD
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]