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The map label for this gene is yhjG [H]

Identifier: 110833521

GI number: 110833521

Start: 730868

End: 732436

Strand: Direct

Name: yhjG [H]

Synonym: ABO_0660

Alternate gene names: 110833521

Gene position: 730868-732436 (Clockwise)

Preceding gene: 110833519

Following gene: 110833524

Centisome position: 23.42

GC content: 58.32

Gene sequence:

>1569_bases
TTGCGCCGTCATGGCATTGATTGTTGCATTATTGACAGGCTGGATGCGCCATCGCCGTATTCGCGCGCCTTGGGGTTACA
TGCTCGAACGCTGGAGATTTTCGATGCGCTGGAGGTGCTGCCGTCGATTCGTAAGGTGTCGAGGCGGCTCAAGGCGGTAT
CAGTGTATGGCGATAAGGGTTTTCTCTTGGAGTTGGATCTCACCACTTTGAAAGCGCCATACCCGTGGGTGCTCAGTTGC
CCGCAATCCGAGGTGGAAACCGTGCTGATTGATCGTTACCGGATGCTGGGGGGGGAATTGATCCGCAGCGCGGAGCTGCT
GGATTTTCGTCAGGATGGTCGCGGCGTCGAGGCGCGTATTCGTCAGGGTTCGGATGTGCAGAAGCTGCAGTGCGAATTGC
TGGTCGGTGCCGATGGTGTGGGTTCTATCGTCCGGGAACAGCTGGGCATTGGTTTCAATGGCGTGGATTATAAGGAACAT
TTCCTGTTGGCGGATGTGCTCTGGGAGGCCCCTTGGAGTAATGATCGCTCTCACGGGTTCCTGCAGGAAGAAGGCTTGTT
ATTGGCATTGCCGCTGCCGCAGGGCTGGCGGTTGATTATGGCCCGTCGCGAGCCGCCGACATTGCCGTTAAGTTTAGCTC
TCTTCACTGAACGGCTCACGTCGATTCTGGGGGAGGCACCGAATCTGGGTGAACCCCGTTGGCTCAGCCAGTTTTCCGTG
CAGCGCCGGCTGGCCCAGCATTTTCGCCGCAACCGGGTGTTTCTGGCCGGGGATGCGGCCCATGTGCAAAGCCCGCTGGG
CGCACAAGGGATGAATACGGGCCTGGCTGATGCCTTCAACCTGAGCTGGAAACTGGCGTTTTATCTGAAAGGATTTGGGC
AGGGCGCTTTGCTAGACAGTTATGAGCAGGAACGGCGCCCGGTGGCGGAGAAAATGTTGTACGGCGTGGACATGCTATCG
CGGGCTTCATTGGTGAAGTTGCCGATGCTGCGACGCAGCCGGGATTCGCTGCTGAAACTGGCGGGTAGCCGTCGTAATAT
CTCCAGTCGTTTGTTGCGCACTGCCAGTCAGTTGAATGTGCACTATCGCAACTCGCCGCTGGTTACCTATGGCCCGGAAG
CGGATGTTGGTTGGCGGCATCAAGGGCCGCTACCGGGGGATCGGCTACCGGATGCGGCGCTGAAATCCGTGCGGACCGGG
CATCTGCATCAGTTGCATGGCTTGCTGCGTAAGCCCGTGCATCATTTGTTATTGCAGCTGAGTGAGAGTCCTGAGCATAA
CGAGCGGCTGGTGATGTATGCGCTGAGTGAGCGACTCGGGCAAGAGTATCGCGAAAGCGTGCATCTGACCGTGATTGCGG
CGCGTGCCACCGCCGATGATGACGTGCCCCGTGAAGGCACCACCCGTATCTGGCGTGACCATGAGGGCGAGTTCGCTAGC
GCCTTTGGCGATAAAGGCCGGCTTTGGTTGATGCGGCCGGATGGGCATTTAGCGTACCGGGCACCGGTGAGCAATGCGGA
TGATTTGCTGGGTTATCTGAGGAGAGTGGTTGGGAAGGCTAACAGCTGA

Upstream 100 bases:

>100_bases
AGTGACATAAGGAGAGTGTACCATCGCCGTTACCGAGAACCGCACAGCGCAGGTATTGATTGTTGGTGCCGGCCCTACTG
GGTTGGCGCTGGGTATTGGT

Downstream 100 bases:

>100_bases
AACGAAGAAGGAGCGAGGTGTTTCTCGTTAGCCCCAAAGGCGTAATCGGACACCTCACTTTATCGAATACGAGAGCGACT
ACGCCACACGCCGCCAGCGT

Product: oxidoreductase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MRRHGIDCCIIDRLDAPSPYSRALGLHARTLEIFDALEVLPSIRKVSRRLKAVSVYGDKGFLLELDLTTLKAPYPWVLSC
PQSEVETVLIDRYRMLGGELIRSAELLDFRQDGRGVEARIRQGSDVQKLQCELLVGADGVGSIVREQLGIGFNGVDYKEH
FLLADVLWEAPWSNDRSHGFLQEEGLLLALPLPQGWRLIMARREPPTLPLSLALFTERLTSILGEAPNLGEPRWLSQFSV
QRRLAQHFRRNRVFLAGDAAHVQSPLGAQGMNTGLADAFNLSWKLAFYLKGFGQGALLDSYEQERRPVAEKMLYGVDMLS
RASLVKLPMLRRSRDSLLKLAGSRRNISSRLLRTASQLNVHYRNSPLVTYGPEADVGWRHQGPLPGDRLPDAALKSVRTG
HLHQLHGLLRKPVHHLLLQLSESPEHNERLVMYALSERLGQEYRESVHLTVIAARATADDDVPREGTTRIWRDHEGEFAS
AFGDKGRLWLMRPDGHLAYRAPVSNADDLLGYLRRVVGKANS

Sequences:

>Translated_522_residues
MRRHGIDCCIIDRLDAPSPYSRALGLHARTLEIFDALEVLPSIRKVSRRLKAVSVYGDKGFLLELDLTTLKAPYPWVLSC
PQSEVETVLIDRYRMLGGELIRSAELLDFRQDGRGVEARIRQGSDVQKLQCELLVGADGVGSIVREQLGIGFNGVDYKEH
FLLADVLWEAPWSNDRSHGFLQEEGLLLALPLPQGWRLIMARREPPTLPLSLALFTERLTSILGEAPNLGEPRWLSQFSV
QRRLAQHFRRNRVFLAGDAAHVQSPLGAQGMNTGLADAFNLSWKLAFYLKGFGQGALLDSYEQERRPVAEKMLYGVDMLS
RASLVKLPMLRRSRDSLLKLAGSRRNISSRLLRTASQLNVHYRNSPLVTYGPEADVGWRHQGPLPGDRLPDAALKSVRTG
HLHQLHGLLRKPVHHLLLQLSESPEHNERLVMYALSERLGQEYRESVHLTVIAARATADDDVPREGTTRIWRDHEGEFAS
AFGDKGRLWLMRPDGHLAYRAPVSNADDLLGYLRRVVGKANS
>Mature_522_residues
MRRHGIDCCIIDRLDAPSPYSRALGLHARTLEIFDALEVLPSIRKVSRRLKAVSVYGDKGFLLELDLTTLKAPYPWVLSC
PQSEVETVLIDRYRMLGGELIRSAELLDFRQDGRGVEARIRQGSDVQKLQCELLVGADGVGSIVREQLGIGFNGVDYKEH
FLLADVLWEAPWSNDRSHGFLQEEGLLLALPLPQGWRLIMARREPPTLPLSLALFTERLTSILGEAPNLGEPRWLSQFSV
QRRLAQHFRRNRVFLAGDAAHVQSPLGAQGMNTGLADAFNLSWKLAFYLKGFGQGALLDSYEQERRPVAEKMLYGVDMLS
RASLVKLPMLRRSRDSLLKLAGSRRNISSRLLRTASQLNVHYRNSPLVTYGPEADVGWRHQGPLPGDRLPDAALKSVRTG
HLHQLHGLLRKPVHHLLLQLSESPEHNERLVMYALSERLGQEYRESVHLTVIAARATADDDVPREGTTRIWRDHEGEFAS
AFGDKGRLWLMRPDGHLAYRAPVSNADDLLGYLRRVVGKANS

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=324, Percent_Identity=27.7777777777778, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789273, Length=256, Percent_Identity=30.078125, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 58716; Mature: 58716

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRHGIDCCIIDRLDAPSPYSRALGLHARTLEIFDALEVLPSIRKVSRRLKAVSVYGDKG
CCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
FLLELDLTTLKAPYPWVLSCPQSEVETVLIDRYRMLGGELIRSAELLDFRQDGRGVEARI
EEEEEEHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RQGSDVQKLQCELLVGADGVGSIVREQLGIGFNGVDYKEHFLLADVLWEAPWSNDRSHGF
CCCCCHHHEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
LQEEGLLLALPLPQGWRLIMARREPPTLPLSLALFTERLTSILGEAPNLGEPRWLSQFSV
CCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
QRRLAQHFRRNRVFLAGDAAHVQSPLGAQGMNTGLADAFNLSWKLAFYLKGFGQGALLDS
HHHHHHHHHHCEEEEECCHHHHCCCCCCCCCCCCCCHHHCCCEEEEEEEECCCCCCHHHH
YEQERRPVAEKMLYGVDMLSRASLVKLPMLRRSRDSLLKLAGSRRNISSRLLRTASQLNV
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEE
HYRNSPLVTYGPEADVGWRHQGPLPGDRLPDAALKSVRTGHLHQLHGLLRKPVHHLLLQL
EECCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SESPEHNERLVMYALSERLGQEYRESVHLTVIAARATADDDVPREGTTRIWRDHEGEFAS
CCCCCCCCCEEHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCCHHHCCCCCCHHH
AFGDKGRLWLMRPDGHLAYRAPVSNADDLLGYLRRVVGKANS
HCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRRHGIDCCIIDRLDAPSPYSRALGLHARTLEIFDALEVLPSIRKVSRRLKAVSVYGDKG
CCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
FLLELDLTTLKAPYPWVLSCPQSEVETVLIDRYRMLGGELIRSAELLDFRQDGRGVEARI
EEEEEEHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RQGSDVQKLQCELLVGADGVGSIVREQLGIGFNGVDYKEHFLLADVLWEAPWSNDRSHGF
CCCCCHHHEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
LQEEGLLLALPLPQGWRLIMARREPPTLPLSLALFTERLTSILGEAPNLGEPRWLSQFSV
CCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
QRRLAQHFRRNRVFLAGDAAHVQSPLGAQGMNTGLADAFNLSWKLAFYLKGFGQGALLDS
HHHHHHHHHHCEEEEECCHHHHCCCCCCCCCCCCCCHHHCCCEEEEEEEECCCCCCHHHH
YEQERRPVAEKMLYGVDMLSRASLVKLPMLRRSRDSLLKLAGSRRNISSRLLRTASQLNV
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCEE
HYRNSPLVTYGPEADVGWRHQGPLPGDRLPDAALKSVRTGHLHQLHGLLRKPVHHLLLQL
EECCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SESPEHNERLVMYALSERLGQEYRESVHLTVIAARATADDDVPREGTTRIWRDHEGEFAS
CCCCCCCCCEEHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCCHHHCCCCCCHHH
AFGDKGRLWLMRPDGHLAYRAPVSNADDLLGYLRRVVGKANS
HCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]