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The map label for this gene is leuA [H]

Identifier: 110833499

GI number: 110833499

Start: 707175

End: 708725

Strand: Direct

Name: leuA [H]

Synonym: ABO_0638

Alternate gene names: 110833499

Gene position: 707175-708725 (Clockwise)

Preceding gene: 110833498

Following gene: 110833500

Centisome position: 22.66

GC content: 57.96

Gene sequence:

>1551_bases
ATGAGCAAAGATCGCCTGATTATTTTTGATACCACCCTGCGTGACGGTGAGCAGAGCCCCGGTGCCACCATGGACCAGGA
TGAAAAACTGCGCATCGCCCGGGCGCTGGAACGCATGCGGGTAGACGTCATCGAAGCCGGCTTCGCCATCGCCAGCCAAG
GGGATTTCCGCTCGGTGAAAGCCATTGCAGACACCGTGCGCGAATCTACCATTTGCTCGCTGGCGCGGGCCAAGGCGGCG
GATATCGACCGCGCGGCGGAAGCACTGGTCGGAGCTGAATCCGGGCGTATCCATACGTTTATCGCCACCAGCCCGATCCA
TATGCAGCATAAGCTGCGTTTGGAGCCGGATCAGGTGGTCGAACATGCGGTGGCCGCGGTGAAGCATGCCCGCAATCATA
TGGGTGATGTGGAGTTTTCCTGTGAAGATGCGGGCCGCTCCGAAATCGATTTCCTGTGCCGCATCATCGAAGCGGTGATT
GATGCGGGTGCGCGTACCATCAACATCCCCGATACCGTGGGTTATGCCATTCCCGAGCAATTTGGCGATACCATTAGCCA
GTTGCTCACGCGCATCCCCAATGCGGATAAGGCGGTGTTTTCCGTGCATTGCCATAACGATTTAGGGTTGGCGGTCGCCA
ACTCTCTGGCAGCGGTTCAGGCGGGTGCGCGGCAGGTGGAATGCACCATCAATGGCCTAGGTGAGCGGGCCGGCAATGCT
TCTCTGGAAGAGATTGTTATGGCGGTGCAAACCCGTCAGGACCTGTTCAATGTGGATACCGGCATTAATGCTCGCGAGAT
CGTGCCCACCTCGAAAATGGTGTCGCAGATTACCGGCTTCCCGGTGCAACCGAACAAGGCCATCGTTGGCGCCAATGCTT
TTGCCCACGAGTCGGGCATTCATCAGGACGGGGTGCTCAAACACCGCGAAACCTACGAAATCATGTCGGCCGAGGATGTG
GGCTGGCACACTAACCGCATGGTGCTGGGCAAGCATTCCGGCCGTGCTGCTTTCCGTGCTCGGCTGGATGAACTGGGGAT
TGTGTTTGCGTCTGAGGCGGCCATGAACGAAGCCTTCTTGCGCTTCAAGGATCTGGCGGACAAGAAGCACGAAGTGTTTG
ATGAGGATCTTCAGGCATTGGTCAGCGATACCAAGCGAGCGGTGGAGCAGGAACGTTTCAAGCTGGTGTTGCTGGAGGCG
ACGGCGCGCACGGGTGAAACACCGCAGGCTCGCATTGTGATGGCTATTGACGGTGTTGAGCAGGACGCCACGGCCACTGG
TGCCGGTCCCGTCGATGCTGCCTTCAAGGCCATTGAGCAGCTGGCAGACAGCCACAGCACCTTGCAGTTGTATTCGGTGA
ACGCGATTACCGCCGGTACTGATAGCCAGGGCGAGGTAACTGTGCGGTTAGAGAAAGGCGGTCGTATCGTCAACGGTCAT
GGTGCCGATGTTGATATTATTACTGCGTCAGCCAAAGCCTACCTGAATGCCCTTAATCACTTCGCCGCCGGCGTAGAAAA
GGCGCATCCCCAGGCGCTAGAGGGAGTATGA

Upstream 100 bases:

>100_bases
GCCGTGCTGCGCACCCTGCGGCTGGTGTAGAATGCGGACATATCTGAACCTGCTGTGAAAAGGGGCCAGCCATTAGCCCC
GGACATTTGCGAGAGATCCA

Downstream 100 bases:

>100_bases
AGGGGATGCTTGGTGTCGGATGTCTGAGGCCTGACGGTAAGGGAGCCGGCGGTGCTCTTCTTGCGTGGTGGGTGGTGCGT
CCGGCGTCTGGCGGTACCGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MSKDRLIIFDTTLRDGEQSPGATMDQDEKLRIARALERMRVDVIEAGFAIASQGDFRSVKAIADTVRESTICSLARAKAA
DIDRAAEALVGAESGRIHTFIATSPIHMQHKLRLEPDQVVEHAVAAVKHARNHMGDVEFSCEDAGRSEIDFLCRIIEAVI
DAGARTINIPDTVGYAIPEQFGDTISQLLTRIPNADKAVFSVHCHNDLGLAVANSLAAVQAGARQVECTINGLGERAGNA
SLEEIVMAVQTRQDLFNVDTGINAREIVPTSKMVSQITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMSAEDV
GWHTNRMVLGKHSGRAAFRARLDELGIVFASEAAMNEAFLRFKDLADKKHEVFDEDLQALVSDTKRAVEQERFKLVLLEA
TARTGETPQARIVMAIDGVEQDATATGAGPVDAAFKAIEQLADSHSTLQLYSVNAITAGTDSQGEVTVRLEKGGRIVNGH
GADVDIITASAKAYLNALNHFAAGVEKAHPQALEGV

Sequences:

>Translated_516_residues
MSKDRLIIFDTTLRDGEQSPGATMDQDEKLRIARALERMRVDVIEAGFAIASQGDFRSVKAIADTVRESTICSLARAKAA
DIDRAAEALVGAESGRIHTFIATSPIHMQHKLRLEPDQVVEHAVAAVKHARNHMGDVEFSCEDAGRSEIDFLCRIIEAVI
DAGARTINIPDTVGYAIPEQFGDTISQLLTRIPNADKAVFSVHCHNDLGLAVANSLAAVQAGARQVECTINGLGERAGNA
SLEEIVMAVQTRQDLFNVDTGINAREIVPTSKMVSQITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMSAEDV
GWHTNRMVLGKHSGRAAFRARLDELGIVFASEAAMNEAFLRFKDLADKKHEVFDEDLQALVSDTKRAVEQERFKLVLLEA
TARTGETPQARIVMAIDGVEQDATATGAGPVDAAFKAIEQLADSHSTLQLYSVNAITAGTDSQGEVTVRLEKGGRIVNGH
GADVDIITASAKAYLNALNHFAAGVEKAHPQALEGV
>Mature_515_residues
SKDRLIIFDTTLRDGEQSPGATMDQDEKLRIARALERMRVDVIEAGFAIASQGDFRSVKAIADTVRESTICSLARAKAAD
IDRAAEALVGAESGRIHTFIATSPIHMQHKLRLEPDQVVEHAVAAVKHARNHMGDVEFSCEDAGRSEIDFLCRIIEAVID
AGARTINIPDTVGYAIPEQFGDTISQLLTRIPNADKAVFSVHCHNDLGLAVANSLAAVQAGARQVECTINGLGERAGNAS
LEEIVMAVQTRQDLFNVDTGINAREIVPTSKMVSQITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMSAEDVG
WHTNRMVLGKHSGRAAFRARLDELGIVFASEAAMNEAFLRFKDLADKKHEVFDEDLQALVSDTKRAVEQERFKLVLLEAT
ARTGETPQARIVMAIDGVEQDATATGAGPVDAAFKAIEQLADSHSTLQLYSVNAITAGTDSQGEVTVRLEKGGRIVNGHG
ADVDIITASAKAYLNALNHFAAGVEKAHPQALEGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=505, Percent_Identity=52.0792079207921, Blast_Score=492, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6320019, Length=360, Percent_Identity=28.6111111111111, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=378, Percent_Identity=28.042328042328, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=536, Percent_Identity=25.7462686567164, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=541, Percent_Identity=25.3234750462107, Blast_Score=121, Evalue=3e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55608; Mature: 55477

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDRLIIFDTTLRDGEQSPGATMDQDEKLRIARALERMRVDVIEAGFAIASQGDFRSVK
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHH
AIADTVRESTICSLARAKAADIDRAAEALVGAESGRIHTFIATSPIHMQHKLRLEPDQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHEECCCHHHHH
EHAVAAVKHARNHMGDVEFSCEDAGRSEIDFLCRIIEAVIDAGARTINIPDTVGYAIPEQ
HHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHCHHCHHH
FGDTISQLLTRIPNADKAVFSVHCHNDLGLAVANSLAAVQAGARQVECTINGLGERAGNA
HHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCC
SLEEIVMAVQTRQDLFNVDTGINAREIVPTSKMVSQITGFPVQPNKAIVGANAFAHESGI
CHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCCCCEEEECCHHHHCCCC
HQDGVLKHRETYEIMSAEDVGWHTNRMVLGKHSGRAAFRARLDELGIVFASEAAMNEAFL
CCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCEEEECHHHHHHHHH
RFKDLADKKHEVFDEDLQALVSDTKRAVEQERFKLVLLEATARTGETPQARIVMAIDGVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEEEEECCC
QDATATGAGPVDAAFKAIEQLADSHSTLQLYSVNAITAGTDSQGEVTVRLEKGGRIVNGH
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEEEEECCCEEEECC
GADVDIITASAKAYLNALNHFAAGVEKAHPQALEGV
CCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
SKDRLIIFDTTLRDGEQSPGATMDQDEKLRIARALERMRVDVIEAGFAIASQGDFRSVK
CCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHH
AIADTVRESTICSLARAKAADIDRAAEALVGAESGRIHTFIATSPIHMQHKLRLEPDQVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHEECCCHHHHH
EHAVAAVKHARNHMGDVEFSCEDAGRSEIDFLCRIIEAVIDAGARTINIPDTVGYAIPEQ
HHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHCHHCHHH
FGDTISQLLTRIPNADKAVFSVHCHNDLGLAVANSLAAVQAGARQVECTINGLGERAGNA
HHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCC
SLEEIVMAVQTRQDLFNVDTGINAREIVPTSKMVSQITGFPVQPNKAIVGANAFAHESGI
CHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHCCCCCCCCCEEEECCHHHHCCCC
HQDGVLKHRETYEIMSAEDVGWHTNRMVLGKHSGRAAFRARLDELGIVFASEAAMNEAFL
CCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCEEEECHHHHHHHHH
RFKDLADKKHEVFDEDLQALVSDTKRAVEQERFKLVLLEATARTGETPQARIVMAIDGVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCEEEEEEEECCC
QDATATGAGPVDAAFKAIEQLADSHSTLQLYSVNAITAGTDSQGEVTVRLEKGGRIVNGH
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEEEEECCCEEEECC
GADVDIITASAKAYLNALNHFAAGVEKAHPQALEGV
CCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA