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The map label for this gene is mepA [H]

Identifier: 110635922

GI number: 110635922

Start: 3877529

End: 3878566

Strand: Reverse

Name: mepA [H]

Synonym: Meso_3596

Alternate gene names: 110635922

Gene position: 3878566-3877529 (Counterclockwise)

Preceding gene: 110635923

Following gene: 110635921

Centisome position: 87.9

GC content: 64.35

Gene sequence:

>1038_bases
ATGCGGCAATTTTCCTTGATGCGATTGATGGGGGCGGGCCTGTTGCTCGGTCTTTCATGGGCCGTTACGGCCAAAGCCGA
GCCGCTGGCCAAGGATCTGTTCGGAGCGCAGAAGCTGCCGGCGGCCACCGCGCCTGCATCCTATGGCTTCTATTCCAAGG
GCTGCTTTGCCGGGGGAATCGCGATTGCCCCCGACGGCAACCATTGGCAGGCCATGCGGCTATCGCGCAATCGGCGCTGG
GGGCATCCCGATCTGATCCACCTGCTCGAACGCTTTTCGCGCGATGCGGCGGCGGATGGCTGGCCTGGCCTTCTGGTCGG
CGATATTTCGCAGCCCCGCGGCGGGCCGATGCTGACGGGGCATGCCTCGCACCAAATCGGGCTGGATGCGGATATCTGGT
TCACGCCCATGCCGAACCGCCGCCTGAGCATCGAGGAGCGCGAGAAGGTCAGCGCCATTTCGATGATCAGGAAAGATTCG
CTCTATGTCGACGAAAACCGTTGGACGGCGGCGCACGCCAATCTGCTGCGTCGGGCGGCGAGCTATCCGCAGGTGGAACG
CATTCTTGTCCATCCCGGCATCAAAAAGAAGCTGTGCGATACCGTGCAAGGCGACCGGAGTTGGCTCAGGAAAATCCGCC
CTTTCTGGGGCCACGATTATCACTTCCATGTCCGCATTGGCTGCCCCAAGGGATCTGTCGGCTGCAAGAGCCAAGCCGCG
GTGCCGCAGAGCGAGGGCTGCGACAAGTCACTCGCCTGGTGGTTCACCGAAGAGCCCTGGAGGCCGGCCACCGGCCCCCA
GGAGCCGAAGGCCCGCGATGTGATGACAATGTCGGCGCTGCCGGCGGCTTGCCGCGTGGTTCTGGCTGCGCCGTCTCCTC
CATCGGAAGCGGCGGTGACGCTGGTGGATGCGGCGGATGCGCCTTCCTACGCGGCTGCGGCGCCAAAGCCGTTGCAACCA
GCGGCAGATCCCGCCTCGCTTCTGCATTCGCTGCAGCCGGCCGATATACCGCGTCCTACGCCGAGGCCTTATCGCTAA

Upstream 100 bases:

>100_bases
TGTGTGCGGCCTTTAGCACACGCGGCGCCCTAAGCTGCCGCAAATATGCTATGATTCGTTTGGACCTTCGTTGCTCGAAA
GAGGCTCGCAAGGAGGCAAG

Downstream 100 bases:

>100_bases
TTTGCATCGGTCGCATGGAACCGCCTTTTCAGCATCGCTCCGCTCACCTATAGAGCTTCAATCGATTTAGGAGCTGAAGC
CTTTGAAAGATCGATGGCAC

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRQFSLMRLMGAGLLLGLSWAVTAKAEPLAKDLFGAQKLPAATAPASYGFYSKGCFAGGIAIAPDGNHWQAMRLSRNRRW
GHPDLIHLLERFSRDAAADGWPGLLVGDISQPRGGPMLTGHASHQIGLDADIWFTPMPNRRLSIEEREKVSAISMIRKDS
LYVDENRWTAAHANLLRRAASYPQVERILVHPGIKKKLCDTVQGDRSWLRKIRPFWGHDYHFHVRIGCPKGSVGCKSQAA
VPQSEGCDKSLAWWFTEEPWRPATGPQEPKARDVMTMSALPAACRVVLAAPSPPSEAAVTLVDAADAPSYAAAAPKPLQP
AADPASLLHSLQPADIPRPTPRPYR

Sequences:

>Translated_345_residues
MRQFSLMRLMGAGLLLGLSWAVTAKAEPLAKDLFGAQKLPAATAPASYGFYSKGCFAGGIAIAPDGNHWQAMRLSRNRRW
GHPDLIHLLERFSRDAAADGWPGLLVGDISQPRGGPMLTGHASHQIGLDADIWFTPMPNRRLSIEEREKVSAISMIRKDS
LYVDENRWTAAHANLLRRAASYPQVERILVHPGIKKKLCDTVQGDRSWLRKIRPFWGHDYHFHVRIGCPKGSVGCKSQAA
VPQSEGCDKSLAWWFTEEPWRPATGPQEPKARDVMTMSALPAACRVVLAAPSPPSEAAVTLVDAADAPSYAAAAPKPLQP
AADPASLLHSLQPADIPRPTPRPYR
>Mature_345_residues
MRQFSLMRLMGAGLLLGLSWAVTAKAEPLAKDLFGAQKLPAATAPASYGFYSKGCFAGGIAIAPDGNHWQAMRLSRNRRW
GHPDLIHLLERFSRDAAADGWPGLLVGDISQPRGGPMLTGHASHQIGLDADIWFTPMPNRRLSIEEREKVSAISMIRKDS
LYVDENRWTAAHANLLRRAASYPQVERILVHPGIKKKLCDTVQGDRSWLRKIRPFWGHDYHFHVRIGCPKGSVGCKSQAA
VPQSEGCDKSLAWWFTEEPWRPATGPQEPKARDVMTMSALPAACRVVLAAPSPPSEAAVTLVDAADAPSYAAAAPKPLQP
AADPASLLHSLQPADIPRPTPRPYR

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=249, Percent_Identity=36.5461847389558, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 37763; Mature: 37763

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQFSLMRLMGAGLLLGLSWAVTAKAEPLAKDLFGAQKLPAATAPASYGFYSKGCFAGGI
CCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCE
AIAPDGNHWQAMRLSRNRRWGHPDLIHLLERFSRDAAADGWPGLLVGDISQPRGGPMLTG
EECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEC
HASHQIGLDADIWFTPMPNRRLSIEEREKVSAISMIRKDSLYVDENRWTAAHANLLRRAA
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
SYPQVERILVHPGIKKKLCDTVQGDRSWLRKIRPFWGHDYHFHVRIGCPKGSVGCKSQAA
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHCCCEEEEEEEECCCCCCCCCCCCC
VPQSEGCDKSLAWWFTEEPWRPATGPQEPKARDVMTMSALPAACRVVLAAPSPPSEAAVT
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCEEE
LVDAADAPSYAAAAPKPLQPAADPASLLHSLQPADIPRPTPRPYR
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRQFSLMRLMGAGLLLGLSWAVTAKAEPLAKDLFGAQKLPAATAPASYGFYSKGCFAGGI
CCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHCHHCCCCCCCCCCCCCCCCCCCCCCE
AIAPDGNHWQAMRLSRNRRWGHPDLIHLLERFSRDAAADGWPGLLVGDISQPRGGPMLTG
EECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEC
HASHQIGLDADIWFTPMPNRRLSIEEREKVSAISMIRKDSLYVDENRWTAAHANLLRRAA
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
SYPQVERILVHPGIKKKLCDTVQGDRSWLRKIRPFWGHDYHFHVRIGCPKGSVGCKSQAA
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHCCCEEEEEEEECCCCCCCCCCCCC
VPQSEGCDKSLAWWFTEEPWRPATGPQEPKARDVMTMSALPAACRVVLAAPSPPSEAAVT
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCEEE
LVDAADAPSYAAAAPKPLQPAADPASLLHSLQPADIPRPTPRPYR
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]