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The map label for this gene is gshB [H]

Identifier: 110635911

GI number: 110635911

Start: 3864796

End: 3865746

Strand: Reverse

Name: gshB [H]

Synonym: Meso_3585

Alternate gene names: 110635911

Gene position: 3865746-3864796 (Counterclockwise)

Preceding gene: 110635917

Following gene: 110635910

Centisome position: 87.61

GC content: 61.2

Gene sequence:

>951_bases
ATGCCGCTTCGCGTCGCCATTCAAATGGACCATGTCTCGACCATCTCAATCGCAGGCGATACTACATTTGCGCTTGCGTT
GGAGGCCGAACGCCGTGGTCACAAGCTGTGGCATTACACGCCCGACCGCCTGTTTCAGCGCGGCAGCGAAGCGTTCGCGA
CGGTGGAGGCGCTGAATGTACGGGACGAGATCGGCAATCACTTTTCCCTAGGCGCGCCCGAACGCATTGCGCTGTCCGAA
ATGGATGTCGTGCTGCTGCGCCAGGATCCGCCCTTCGACATGAATTACATCACCACCACGCATATACTCGAGCGCGTCCA
CCCCAAGACATTGGTGGTCAATGATCCGGTCTGGGTTCGCAACAGCCCGGAAAAGATTTTCGTGACGGAGTTTCCGGATT
TGATGCCGGACACGCTGATCACCCGCGATCCGATCGAGGTCGACGCGTTCCGGAGGGAACATGGCGACATCATCGTCAAG
CCGCTCTACGGCAATGGCGGCGCGGGCATATTCCACCTGGCGCAGGCAGACCGCAATCTCGCCTCTCTGCTCGAGCTGTT
CACCCAGATGTTCCGCGAGCCCTTCATCGTGCAACGCTACCTGAAGGAGGTGCGCGGCGGTGACAAGCGCATCATCCTGA
TCGAAGGCGAACCGGTGGGGGCCATCAATCGCATACCCTCGGAACATGATTCGCGCTCCAACATGCATGTGGGCGGACGC
GCGGAACCGACGGAAATCACCGAGCGCGAGCGCGAAATCTGCGCCCGCATCGGGCCAGCGCTGCGCGAGCGCGGCTTTGT
TCTGGTGGGGATCGACGTGATCGGCGGTCTCCTCACGGAGATCAATGTCACTTCACCGACGGGGGTGCGGGAGGTGAAAC
GATTTGGCGGCGCCGACATCGCTGCTCTTTTCTGGGATGCGGTGGAGGCGCGGCGCGGCGCGATTTCGTAG

Upstream 100 bases:

>100_bases
TGTTTCCGCTGCCTGACACGGCTTCCGCAGGCGCTTGACAGTTGGACGATGGCGGCGTCATCTGCCGCGAATCGCTGCCG
CAACCTGCCGGAGTCCTTTT

Downstream 100 bases:

>100_bases
GACGGCGGACCTTCTCCGCTCAGCCGCGGTAACCCCGTCCGTTATGCATTCATAAACCGGCCGTGTGAGATAGCCTGGGC
TGCCGTGAAGCGGTTCGCGT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPLRVAIQMDHVSTISIAGDTTFALALEAERRGHKLWHYTPDRLFQRGSEAFATVEALNVRDEIGNHFSLGAPERIALSE
MDVVLLRQDPPFDMNYITTTHILERVHPKTLVVNDPVWVRNSPEKIFVTEFPDLMPDTLITRDPIEVDAFRREHGDIIVK
PLYGNGGAGIFHLAQADRNLASLLELFTQMFREPFIVQRYLKEVRGGDKRIILIEGEPVGAINRIPSEHDSRSNMHVGGR
AEPTEITEREREICARIGPALRERGFVLVGIDVIGGLLTEINVTSPTGVREVKRFGGADIAALFWDAVEARRGAIS

Sequences:

>Translated_316_residues
MPLRVAIQMDHVSTISIAGDTTFALALEAERRGHKLWHYTPDRLFQRGSEAFATVEALNVRDEIGNHFSLGAPERIALSE
MDVVLLRQDPPFDMNYITTTHILERVHPKTLVVNDPVWVRNSPEKIFVTEFPDLMPDTLITRDPIEVDAFRREHGDIIVK
PLYGNGGAGIFHLAQADRNLASLLELFTQMFREPFIVQRYLKEVRGGDKRIILIEGEPVGAINRIPSEHDSRSNMHVGGR
AEPTEITEREREICARIGPALRERGFVLVGIDVIGGLLTEINVTSPTGVREVKRFGGADIAALFWDAVEARRGAIS
>Mature_315_residues
PLRVAIQMDHVSTISIAGDTTFALALEAERRGHKLWHYTPDRLFQRGSEAFATVEALNVRDEIGNHFSLGAPERIALSEM
DVVLLRQDPPFDMNYITTTHILERVHPKTLVVNDPVWVRNSPEKIFVTEFPDLMPDTLITRDPIEVDAFRREHGDIIVKP
LYGNGGAGIFHLAQADRNLASLLELFTQMFREPFIVQRYLKEVRGGDKRIILIEGEPVGAINRIPSEHDSRSNMHVGGRA
EPTEITEREREICARIGPALRERGFVLVGIDVIGGLLTEINVTSPTGVREVKRFGGADIAALFWDAVEARRGAIS

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=311, Percent_Identity=42.1221864951768, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1787076, Length=149, Percent_Identity=34.8993288590604, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35401; Mature: 35270

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRVAIQMDHVSTISIAGDTTFALALEAERRGHKLWHYTPDRLFQRGSEAFATVEALNV
CCEEEEEEECCCEEEEECCCCEEEEEEECCCCCCEEEEECHHHHHHCCCHHHHHHHHHHH
RDEIGNHFSLGAPERIALSEMDVVLLRQDPPFDMNYITTTHILERVHPKTLVVNDPVWVR
HHHHCCCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCEEEC
NSPEKIFVTEFPDLMPDTLITRDPIEVDAFRREHGDIIVKPLYGNGGAGIFHLAQADRNL
CCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHCCHH
ASLLELFTQMFREPFIVQRYLKEVRGGDKRIILIEGEPVGAINRIPSEHDSRSNMHVGGR
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCCCCCCCEEECCC
AEPTEITEREREICARIGPALRERGFVLVGIDVIGGLLTEINVTSPTGVREVKRFGGADI
CCCHHHHHHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHHCCCHH
AALFWDAVEARRGAIS
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLRVAIQMDHVSTISIAGDTTFALALEAERRGHKLWHYTPDRLFQRGSEAFATVEALNV
CEEEEEEECCCEEEEECCCCEEEEEEECCCCCCEEEEECHHHHHHCCCHHHHHHHHHHH
RDEIGNHFSLGAPERIALSEMDVVLLRQDPPFDMNYITTTHILERVHPKTLVVNDPVWVR
HHHHCCCCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCEEEC
NSPEKIFVTEFPDLMPDTLITRDPIEVDAFRREHGDIIVKPLYGNGGAGIFHLAQADRNL
CCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCEEEEHHHCCHH
ASLLELFTQMFREPFIVQRYLKEVRGGDKRIILIEGEPVGAINRIPSEHDSRSNMHVGGR
HHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCCCCCCCEEECCC
AEPTEITEREREICARIGPALRERGFVLVGIDVIGGLLTEINVTSPTGVREVKRFGGADI
CCCHHHHHHHHHHHHHHCHHHHHCCEEEEEHHHHHHHHHEEECCCCCCHHHHHHHCCCHH
AALFWDAVEARRGAIS
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]