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The map label for this gene is shiA [H]

Identifier: 110635875

GI number: 110635875

Start: 3821762

End: 3823084

Strand: Reverse

Name: shiA [H]

Synonym: Meso_3547

Alternate gene names: 110635875

Gene position: 3823084-3821762 (Counterclockwise)

Preceding gene: 110635876

Following gene: 110635874

Centisome position: 86.64

GC content: 63.87

Gene sequence:

>1323_bases
ATGACAATGTCCATCGCGGTGGGGAAGAGCCCCGCCGAGCAGGCCAACATTCGGCGCGCCGTCACGGCGGCGACCATCGG
CACGATTATCGAATGGTTCGACTATGCGCTCTACGGCGCCGCGGCCGGGCTGATCATCAACAAGCTGTTCTTCCCGCAAT
TCTCGGAATCGGCCGGCGTGCTGGCGGCTTTCGCCACCTTCGCCGTCGGTTTCTTCAGCCGGCCGCTCGGCGGCGTGCTG
ATCTCGCATATGGGTGACCGCTTCGGCCGCAAGCCGGCGCTGATCTTCACCATATCGCTGATGGGCCTCACCACCGTTGC
GATGGGCTTGCTGCCGACCTATGCGCAGGTCGGCCTCTGGGCGCCCGCCCTGCTGGTTTTGCTGCGCCTGCTCCAGGGTT
TCGGCGCCGGTGCCGAATATGCCGGCGCGGTGACGCTTGTTGCCGAATACGTTCCGCCGGAGCGCAAGGCCTATTACACC
GCTTACCTCCAGGCGGCGACGGTGGTCGGCATCATGCTGGCGACGCTCATGTTCCTGCTCGTATCCTACCTGCCGGAGGA
GACGCTGTTCGGCTGGGCTTGGCGCGTGCCCTTCCTGATCTCTGCGCTGCTTTTCGGCGTGGCGCTCTACATCCGCAAGA
ACCTCGATGAGACGCCGGAATATGTCGCTGCCATGAAGAAAGCGGCAGAGCGACGTCACGAGCAGCGCGTGCCAATCGGC
GAGTTGATCACGAAGAGCCCGCGCGAATTGCTGTTCGGCTTCCTGTCGGTCACCGGCCACAACGCCAACGCCTACATCCT
GAGCGCCTTCAGCTTGAGCTACATGACCAACACGCTCGGCATGACCCGCACCGACTCACTGACCGCGGTTATGATCGCGA
CGCTTACAGGCATCATTATGACGCCGCCGATGGGCGCTCTCGCCGATCGCATCGGCCATGCGCGGGTTTATTTCTTCGGC
GCGGTCTTCGTCTTCTTCTTTGCCTTCCCGATGTTCGCGATGCTCGATACGAAGAACGTCGTCCTCGCCACGCTCGCGCT
CAGCATCGGCTACGGCATCGGCTTCGGCGGGCTCGCCAGCGCGCAGGGTGCCTTCCTTGCCAACCTCTTCCCGGTTCGCT
ACCGGTTCTCCGGTATCGCCATGACCCGAGAGCTGAACAGCCTGCTCATCGCCGGTCCGACGCCTTTCATCGCCTCGGCG
CTGGTCGGCTGGGGCGGCGGCGAACCGACGCTGGTCGTCGGCTATTTGATGCTGTGCTGTGCAGTAACCGCGATCGCGAT
CCTCGCGGTCCGCTCGCGATCCTTCGAACGCGGCTCCACCTGA

Upstream 100 bases:

>100_bases
CGGTCTTGCCGAAAGCGCGAGCCGCTTCGGCCTCACCATGCCCGCCGCCGTTTCCTGACGGCGGTCACCATCAACGACAA
TAACGTTTGGAGGAATTCCC

Downstream 100 bases:

>100_bases
AGCCGCGGCAGGAGGTGTCTGACCGCCTTCGGTTCGTGCACCCCGTGCATACCGGCGATACGATCTGCAGGGGGTGACGA
CCAGCGCCCAGGAAACGGCC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTMSIAVGKSPAEQANIRRAVTAATIGTIIEWFDYALYGAAAGLIINKLFFPQFSESAGVLAAFATFAVGFFSRPLGGVL
ISHMGDRFGRKPALIFTISLMGLTTVAMGLLPTYAQVGLWAPALLVLLRLLQGFGAGAEYAGAVTLVAEYVPPERKAYYT
AYLQAATVVGIMLATLMFLLVSYLPEETLFGWAWRVPFLISALLFGVALYIRKNLDETPEYVAAMKKAAERRHEQRVPIG
ELITKSPRELLFGFLSVTGHNANAYILSAFSLSYMTNTLGMTRTDSLTAVMIATLTGIIMTPPMGALADRIGHARVYFFG
AVFVFFFAFPMFAMLDTKNVVLATLALSIGYGIGFGGLASAQGAFLANLFPVRYRFSGIAMTRELNSLLIAGPTPFIASA
LVGWGGGEPTLVVGYLMLCCAVTAIAILAVRSRSFERGST

Sequences:

>Translated_440_residues
MTMSIAVGKSPAEQANIRRAVTAATIGTIIEWFDYALYGAAAGLIINKLFFPQFSESAGVLAAFATFAVGFFSRPLGGVL
ISHMGDRFGRKPALIFTISLMGLTTVAMGLLPTYAQVGLWAPALLVLLRLLQGFGAGAEYAGAVTLVAEYVPPERKAYYT
AYLQAATVVGIMLATLMFLLVSYLPEETLFGWAWRVPFLISALLFGVALYIRKNLDETPEYVAAMKKAAERRHEQRVPIG
ELITKSPRELLFGFLSVTGHNANAYILSAFSLSYMTNTLGMTRTDSLTAVMIATLTGIIMTPPMGALADRIGHARVYFFG
AVFVFFFAFPMFAMLDTKNVVLATLALSIGYGIGFGGLASAQGAFLANLFPVRYRFSGIAMTRELNSLLIAGPTPFIASA
LVGWGGGEPTLVVGYLMLCCAVTAIAILAVRSRSFERGST
>Mature_439_residues
TMSIAVGKSPAEQANIRRAVTAATIGTIIEWFDYALYGAAAGLIINKLFFPQFSESAGVLAAFATFAVGFFSRPLGGVLI
SHMGDRFGRKPALIFTISLMGLTTVAMGLLPTYAQVGLWAPALLVLLRLLQGFGAGAEYAGAVTLVAEYVPPERKAYYTA
YLQAATVVGIMLATLMFLLVSYLPEETLFGWAWRVPFLISALLFGVALYIRKNLDETPEYVAAMKKAAERRHEQRVPIGE
LITKSPRELLFGFLSVTGHNANAYILSAFSLSYMTNTLGMTRTDSLTAVMIATLTGIIMTPPMGALADRIGHARVYFFGA
VFVFFFAFPMFAMLDTKNVVLATLALSIGYGIGFGGLASAQGAFLANLFPVRYRFSGIAMTRELNSLLIAGPTPFIASAL
VGWGGGEPTLVVGYLMLCCAVTAIAILAVRSRSFERGST

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=34.8557692307692, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1790550, Length=397, Percent_Identity=33.5012594458438, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=34.5070422535211, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1788942, Length=410, Percent_Identity=28.0487804878049, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI87082231, Length=304, Percent_Identity=26.9736842105263, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=24.869109947644, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47266; Mature: 47135

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSIAVGKSPAEQANIRRAVTAATIGTIIEWFDYALYGAAAGLIINKLFFPQFSESAGV
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
LAAFATFAVGFFSRPLGGVLISHMGDRFGRKPALIFTISLMGLTTVAMGLLPTYAQVGLW
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
APALLVLLRLLQGFGAGAEYAGAVTLVAEYVPPERKAYYTAYLQAATVVGIMLATLMFLL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSYLPEETLFGWAWRVPFLISALLFGVALYIRKNLDETPEYVAAMKKAAERRHEQRVPIG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHH
ELITKSPRELLFGFLSVTGHNANAYILSAFSLSYMTNTLGMTRTDSLTAVMIATLTGIIM
HHHCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
TPPMGALADRIGHARVYFFGAVFVFFFAFPMFAMLDTKNVVLATLALSIGYGIGFGGLAS
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHH
AQGAFLANLFPVRYRFSGIAMTRELNSLLIAGPTPFIASALVGWGGGEPTLVVGYLMLCC
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHH
AVTAIAILAVRSRSFERGST
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TMSIAVGKSPAEQANIRRAVTAATIGTIIEWFDYALYGAAAGLIINKLFFPQFSESAGV
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
LAAFATFAVGFFSRPLGGVLISHMGDRFGRKPALIFTISLMGLTTVAMGLLPTYAQVGLW
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
APALLVLLRLLQGFGAGAEYAGAVTLVAEYVPPERKAYYTAYLQAATVVGIMLATLMFLL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSYLPEETLFGWAWRVPFLISALLFGVALYIRKNLDETPEYVAAMKKAAERRHEQRVPIG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHH
ELITKSPRELLFGFLSVTGHNANAYILSAFSLSYMTNTLGMTRTDSLTAVMIATLTGIIM
HHHCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
TPPMGALADRIGHARVYFFGAVFVFFFAFPMFAMLDTKNVVLATLALSIGYGIGFGGLAS
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHH
AQGAFLANLFPVRYRFSGIAMTRELNSLLIAGPTPFIASALVGWGGGEPTLVVGYLMLCC
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHH
AVTAIAILAVRSRSFERGST
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]