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The map label for this gene is pckA

Identifier: 110635830

GI number: 110635830

Start: 3772579

End: 3774189

Strand: Reverse

Name: pckA

Synonym: Meso_3502

Alternate gene names: 110635830

Gene position: 3774189-3772579 (Counterclockwise)

Preceding gene: 110635831

Following gene: 110635829

Centisome position: 85.54

GC content: 60.96

Gene sequence:

>1611_bases
ATGCAAGAGACCGGCCTCCGCAACCCCACCAACGGCATCGAGACCAGTGGTTTGAAGACCAGCGGGACCGTTTTCTTCAA
TCTCACCGAGCCGCAACTTTATGAGGAGGCGATCCGGCGCGCCGAGGCGCGGATTTCCGCTCACGGGGCCCTCGTTGCCG
AGACGGGACAGCACACCGGCCGCTCGCCCAAGGACAAGTTCATTGTGCGAGATGCCAGCACGGAAAACGAGATCTGGTGG
GACAACAATAAGCCGCTATCGGGCGAACATTTTGAAGCACTCTACGAGGACTTCAAGCGTCACGCCGAGAGCCTTGACCT
TTTCGTACAGGATCTCGTCGGCGGGGCTGATCCGGAAAACGCGCTGCCGGTGCGCGTCGTCACGGAATATGCCTGGCATT
CGCTCTTCATCCGCAATCTGCTGATCCGCCCGATGCGCTCGGAGCTCAAGAGCTTCGTGCCGCAGATGACGGTGATCGAT
CTGCCGTCCTTCCGCGCCGATCCGAAGCGGCATGGCTGCCGCACGGAAACCATTATCGCGATGGATCTGAAGCGCATGAT
CGTGCTGATCGGCGGCACGTCCTATGCGGGGGAAATGAAAAAATCCGTCTTCACTGCGCTCAATTACCTGCTTCCGGCCA
AGGGCGTGATGCCCATGCACTGCTCGGCCAATGAGGGATCCGAGGGCGACGTTGCGATCTTCTTCGGCCTGTCGGGCACG
GGAAAGACGACCCTTTCCGCCGAACCTTCGCGCACGCTTATCGGCGATGACGAGCACGGGTGGGGGCCGCACGGCATCTT
CAATTTCGAAGGCGGGTGCTACGCCAAGACGATCAAGCTCAGCCGGGAGGCGGAGCCGGAGATTTTCGCCACCACGGAAC
GCTTCGGCACGGTGCTGGAAAATGTGGTCGTCGACGAGCACGGCGTTCCCGATTTCGACGATGGCAGCCGGACCGAGAAC
ACGCGCTGCGCATATCCGCTGCACTTCATCTCCAATGCCAGCGAGACGGGCAGGGCAGGGCAGCCAAAGAACGTCATCAT
GCTGACGGCGGATGCCTTTGGCGTGATGCCACCCATCGCCAAGCTTACCCCTGCCCAGGCCATGTACCATTTCCTGTCGG
GCTACACGGCGAAAGTGGCGGGAACGGAAAAAGGCGTCACCGAGCCGGAGGCCACCTTCTCCACCTGTTTCGGCGCACCC
TTCATGCCCCGCCATCCTTCGGTCTACGGAAATCTTCTCAGAGACCTGATCGCAGAGCACGAGGTCGACTGCTGGCTAGT
CAATACTGGTTGGACCGGCGGCGCCTACGGCACTGGCCACCGCATGCCCATCAAGGTGACACGCGCCCTGCTCTCCGCTG
CACTCGACGGCTCGCTTCGAACAGCCCAGTTCCGTACCGACCCCAATTTTGGCTTTGCCGTACCCGTGGCGGTGCCGGGC
GTAGACGGCACCATCCTCGATCCGCGCTCAACCTGGGCAGATAAGGCCGCCTATGATGTGCAGGCCGAGCGGCTTGTTTC
CATGTTCGTCGCCAATTTCGGGAAATTCGAGAATCACGTGGAAGCAGACGTGATGAATGCATCGCCTCAGATCCGGCAGG
CTGCTGAATAG

Upstream 100 bases:

>100_bases
TGCTTTTCTGGTCCTGAAGACAGACATAGCCTATTTAACCCCGTCCGTCGGTCACCTCAGGCCGGCTTGTTCCGATTAAC
GCCTGTTCCTGGAGTGACTT

Downstream 100 bases:

>100_bases
GCTTGCTCCCGCGGCGTTGGCGGACCATCTTTCGATGTGAATCGCGGCTGGCAGCTTAGTCCGCCGCTCCGGAACGTCAG
AGCGGGTAGCTATGGACGAC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MQETGLRNPTNGIETSGLKTSGTVFFNLTEPQLYEEAIRRAEARISAHGALVAETGQHTGRSPKDKFIVRDASTENEIWW
DNNKPLSGEHFEALYEDFKRHAESLDLFVQDLVGGADPENALPVRVVTEYAWHSLFIRNLLIRPMRSELKSFVPQMTVID
LPSFRADPKRHGCRTETIIAMDLKRMIVLIGGTSYAGEMKKSVFTALNYLLPAKGVMPMHCSANEGSEGDVAIFFGLSGT
GKTTLSAEPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSREAEPEIFATTERFGTVLENVVVDEHGVPDFDDGSRTEN
TRCAYPLHFISNASETGRAGQPKNVIMLTADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSVYGNLLRDLIAEHEVDCWLVNTGWTGGAYGTGHRMPIKVTRALLSAALDGSLRTAQFRTDPNFGFAVPVAVPG
VDGTILDPRSTWADKAAYDVQAERLVSMFVANFGKFENHVEADVMNASPQIRQAAE

Sequences:

>Translated_536_residues
MQETGLRNPTNGIETSGLKTSGTVFFNLTEPQLYEEAIRRAEARISAHGALVAETGQHTGRSPKDKFIVRDASTENEIWW
DNNKPLSGEHFEALYEDFKRHAESLDLFVQDLVGGADPENALPVRVVTEYAWHSLFIRNLLIRPMRSELKSFVPQMTVID
LPSFRADPKRHGCRTETIIAMDLKRMIVLIGGTSYAGEMKKSVFTALNYLLPAKGVMPMHCSANEGSEGDVAIFFGLSGT
GKTTLSAEPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSREAEPEIFATTERFGTVLENVVVDEHGVPDFDDGSRTEN
TRCAYPLHFISNASETGRAGQPKNVIMLTADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSVYGNLLRDLIAEHEVDCWLVNTGWTGGAYGTGHRMPIKVTRALLSAALDGSLRTAQFRTDPNFGFAVPVAVPG
VDGTILDPRSTWADKAAYDVQAERLVSMFVANFGKFENHVEADVMNASPQIRQAAE
>Mature_536_residues
MQETGLRNPTNGIETSGLKTSGTVFFNLTEPQLYEEAIRRAEARISAHGALVAETGQHTGRSPKDKFIVRDASTENEIWW
DNNKPLSGEHFEALYEDFKRHAESLDLFVQDLVGGADPENALPVRVVTEYAWHSLFIRNLLIRPMRSELKSFVPQMTVID
LPSFRADPKRHGCRTETIIAMDLKRMIVLIGGTSYAGEMKKSVFTALNYLLPAKGVMPMHCSANEGSEGDVAIFFGLSGT
GKTTLSAEPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSREAEPEIFATTERFGTVLENVVVDEHGVPDFDDGSRTEN
TRCAYPLHFISNASETGRAGQPKNVIMLTADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAP
FMPRHPSVYGNLLRDLIAEHEVDCWLVNTGWTGGAYGTGHRMPIKVTRALLSAALDGSLRTAQFRTDPNFGFAVPVAVPG
VDGTILDPRSTWADKAAYDVQAERLVSMFVANFGKFENHVEADVMNASPQIRQAAE

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=534, Percent_Identity=47.565543071161, Blast_Score=489, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6322950, Length=507, Percent_Identity=47.9289940828402, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_MESSB (Q11CK2)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_676038.1
- ProteinModelPortal:   Q11CK2
- SMR:   Q11CK2
- STRING:   Q11CK2
- GeneID:   4182162
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_3502
- NMPDR:   fig|266779.1.peg.1032
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- PhylomeDB:   Q11CK2
- ProtClustDB:   PRK09344
- BioCyc:   MSP266779:MESO_3502-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58741; Mature: 58741

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQETGLRNPTNGIETSGLKTSGTVFFNLTEPQLYEEAIRRAEARISAHGALVAETGQHTG
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
RSPKDKFIVRDASTENEIWWDNNKPLSGEHFEALYEDFKRHAESLDLFVQDLVGGADPEN
CCCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ALPVRVVTEYAWHSLFIRNLLIRPMRSELKSFVPQMTVIDLPSFRADPKRHGCRTETIIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCEEHH
MDLKRMIVLIGGTSYAGEMKKSVFTALNYLLPAKGVMPMHCSANEGSEGDVAIFFGLSGT
EECCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCC
GKTTLSAEPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSREAEPEIFATTERFGTVLE
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHHHH
NVVVDEHGVPDFDDGSRTENTRCAYPLHFISNASETGRAGQPKNVIMLTADAFGVMPPIA
HHHHCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHH
KLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSVYGNLLRDLIAEH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
EVDCWLVNTGWTGGAYGTGHRMPIKVTRALLSAALDGSLRTAQFRTDPNFGFAVPVAVPG
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECC
VDGTILDPRSTWADKAAYDVQAERLVSMFVANFGKFENHVEADVMNASPQIRQAAE
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MQETGLRNPTNGIETSGLKTSGTVFFNLTEPQLYEEAIRRAEARISAHGALVAETGQHTG
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
RSPKDKFIVRDASTENEIWWDNNKPLSGEHFEALYEDFKRHAESLDLFVQDLVGGADPEN
CCCCCCEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ALPVRVVTEYAWHSLFIRNLLIRPMRSELKSFVPQMTVIDLPSFRADPKRHGCRTETIIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCCCCCEEHH
MDLKRMIVLIGGTSYAGEMKKSVFTALNYLLPAKGVMPMHCSANEGSEGDVAIFFGLSGT
EECCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCC
GKTTLSAEPSRTLIGDDEHGWGPHGIFNFEGGCYAKTIKLSREAEPEIFATTERFGTVLE
CCEEECCCCCCCEECCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHHHH
NVVVDEHGVPDFDDGSRTENTRCAYPLHFISNASETGRAGQPKNVIMLTADAFGVMPPIA
HHHHCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHH
KLTPAQAMYHFLSGYTAKVAGTEKGVTEPEATFSTCFGAPFMPRHPSVYGNLLRDLIAEH
HCCHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
EVDCWLVNTGWTGGAYGTGHRMPIKVTRALLSAALDGSLRTAQFRTDPNFGFAVPVAVPG
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECC
VDGTILDPRSTWADKAAYDVQAERLVSMFVANFGKFENHVEADVMNASPQIRQAAE
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA