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The map label for this gene is rho [H]

Identifier: 110635802

GI number: 110635802

Start: 3751923

End: 3753212

Strand: Reverse

Name: rho [H]

Synonym: Meso_3474

Alternate gene names: 110635802

Gene position: 3753212-3751923 (Counterclockwise)

Preceding gene: 110635803

Following gene: 110635800

Centisome position: 85.06

GC content: 55.35

Gene sequence:

>1290_bases
ATGCTACAAGCAGAGTCCGCCCCTATGCAGGAAATGAAACTCCAAGAGCTGAAGAGCAAATCGCCGACCGACCTGATCGC
GTTTGCTGAATCGCTCGAGGTCGAGAACGCCAGCATTATGCGCAAGCAGGAGCTGATGTTTGCGATCCTCAAAAAGCTCT
CAATGCAGGACATAGAGATTGTTGGGGATGGCGTCGTAGAGGTGCTGCAGGATGGCTTTGGCTTTTTGCGTTCAGCCACC
GCCAATTACTTGCCGGGCCCGGACGACATTTATATTTCTCCTTCGCAGATCCGGCGGTTTTCTCTGAAGACAGGCGATAC
GGTAGAGGGTCCCATTCGCAGCCCCAAGGAGGGTGAACGTTATTTCGCCCTGCTTAAGGTGAACACCATCAACTTCGAGG
ACCCGGAGAAGATCCGGCATAAAATCCACTTCGATAACCTTACGCCGCTTTATCCGGATGAGCGGCTGAGGATGGAGATT
GAGAATCCGACTACAAAGGATCTCTCACCGCGGGTGATCGACTTGGTGGCTCCCCTTGGAAAAGGACAACGCGGGCTTAT
CGTTGCTCCGCCACGAACCGGTAAGACCGTTCTGCTCCAGAACATCGCCCACTCAATCACCACGAACCACCCGGAATGCT
ATCTCATCGTGCTGCTTATCGACGAGCGGCCGGAAGAGGTGACCGACATGCAGAGGTCTGTGAAGGGGGAAGTGATTTCC
TCGACCTTTGACGAACCGGCTGCCCGGCATGTCCAGGTAGCAGAGATGGTCATTGAAAAAGCAAAGCGCCTCGTGGAGCA
TGGGCGTGACGTTGTGATCCTGCTTGACTCGATCACGCGCCTCGGCCGCGCCTACAATACGGTGGTGCCCTCCTCCGGAA
AGGTGTTGACCGGCGGTGTGGACGCCAATGCGCTGCAGCGTCCGAAGCGCTTTTTCGGCGCGGCTCGCAATATTGAGGAG
GGTGGCTCGCTTACGATTATCGCCACCGCGCTCATCGATACGGGCAGCCGTATGGACGAGGTGATCTTTGAAGAATTCAA
GGGTACGGGCAACTCGGAAATTGTGCTCGACCGCAAGGTCGCTGATAAGCGCATCTATCCAGCCATGGACATCTTGAAAT
CCGGCACGCGCAAGGAGGATCTCCTCGTGCCGCGTCAGGATTTGCAAAAGATCTTCGTTTTGCGCAGGATCCTGGCTCCG
ATGGGAACAACGGATGCCATCGAGTTCCTCATCGACAAGCTCAAGCAGACCAAGGGCAACGCCGAATTCTTCGATTCCAT
GAATACGTAG

Upstream 100 bases:

>100_bases
AAAGGGCTTTTCCCTCCCTTCATGTCCGGTGCCGTTCTTTACCGAAGTCTCGGACGCTGTAGGCCCGCAAGAGCGCCCAG
CAATCAATCACGGTATCCAT

Downstream 100 bases:

>100_bases
CTAGCGACCGTCTGTCGAGCGCTTCTACCTACTGTTTGTACTTTGGTGCGGCGTTCTCAGCGTCGCTGCCAGGCTGTCCG
CATTATGAATGGGTGGCAGG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLQAESAPMQEMKLQELKSKSPTDLIAFAESLEVENASIMRKQELMFAILKKLSMQDIEIVGDGVVEVLQDGFGFLRSAT
ANYLPGPDDIYISPSQIRRFSLKTGDTVEGPIRSPKEGERYFALLKVNTINFEDPEKIRHKIHFDNLTPLYPDERLRMEI
ENPTTKDLSPRVIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITTNHPECYLIVLLIDERPEEVTDMQRSVKGEVIS
STFDEPAARHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEE
GGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIYPAMDILKSGTRKEDLLVPRQDLQKIFVLRRILAP
MGTTDAIEFLIDKLKQTKGNAEFFDSMNT

Sequences:

>Translated_429_residues
MLQAESAPMQEMKLQELKSKSPTDLIAFAESLEVENASIMRKQELMFAILKKLSMQDIEIVGDGVVEVLQDGFGFLRSAT
ANYLPGPDDIYISPSQIRRFSLKTGDTVEGPIRSPKEGERYFALLKVNTINFEDPEKIRHKIHFDNLTPLYPDERLRMEI
ENPTTKDLSPRVIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITTNHPECYLIVLLIDERPEEVTDMQRSVKGEVIS
STFDEPAARHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEE
GGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIYPAMDILKSGTRKEDLLVPRQDLQKIFVLRRILAP
MGTTDAIEFLIDKLKQTKGNAEFFDSMNT
>Mature_429_residues
MLQAESAPMQEMKLQELKSKSPTDLIAFAESLEVENASIMRKQELMFAILKKLSMQDIEIVGDGVVEVLQDGFGFLRSAT
ANYLPGPDDIYISPSQIRRFSLKTGDTVEGPIRSPKEGERYFALLKVNTINFEDPEKIRHKIHFDNLTPLYPDERLRMEI
ENPTTKDLSPRVIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITTNHPECYLIVLLIDERPEEVTDMQRSVKGEVIS
STFDEPAARHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEE
GGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIYPAMDILKSGTRKEDLLVPRQDLQKIFVLRRILAP
MGTTDAIEFLIDKLKQTKGNAEFFDSMNT

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=69.7115384615385, Blast_Score=613, Evalue=1e-177,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47957; Mature: 47957

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQAESAPMQEMKLQELKSKSPTDLIAFAESLEVENASIMRKQELMFAILKKLSMQDIEI
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHH
VGDGVVEVLQDGFGFLRSATANYLPGPDDIYISPSQIRRFSLKTGDTVEGPIRSPKEGER
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCE
YFALLKVNTINFEDPEKIRHKIHFDNLTPLYPDERLRMEIENPTTKDLSPRVIDLVAPLG
EEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCC
KGQRGLIVAPPRTGKTVLLQNIAHSITTNHPECYLIVLLIDERPEEVTDMQRSVKGEVIS
CCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
STFDEPAARHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGV
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCC
DANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKV
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEECHH
ADKRIYPAMDILKSGTRKEDLLVPRQDLQKIFVLRRILAPMGTTDAIEFLIDKLKQTKGN
HCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AEFFDSMNT
HHHHHCCCC
>Mature Secondary Structure
MLQAESAPMQEMKLQELKSKSPTDLIAFAESLEVENASIMRKQELMFAILKKLSMQDIEI
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHH
VGDGVVEVLQDGFGFLRSATANYLPGPDDIYISPSQIRRFSLKTGDTVEGPIRSPKEGER
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHHHHEEECCCCCCCCCCCCCCCCCCE
YFALLKVNTINFEDPEKIRHKIHFDNLTPLYPDERLRMEIENPTTKDLSPRVIDLVAPLG
EEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCC
KGQRGLIVAPPRTGKTVLLQNIAHSITTNHPECYLIVLLIDERPEEVTDMQRSVKGEVIS
CCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
STFDEPAARHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGV
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCC
DANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKV
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEECHH
ADKRIYPAMDILKSGTRKEDLLVPRQDLQKIFVLRRILAPMGTTDAIEFLIDKLKQTKGN
HCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
AEFFDSMNT
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]