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The map label for this gene is trmE

Identifier: 110635800

GI number: 110635800

Start: 3748488

End: 3749816

Strand: Reverse

Name: trmE

Synonym: Meso_3472

Alternate gene names: 110635800

Gene position: 3749816-3748488 (Counterclockwise)

Preceding gene: 110635802

Following gene: 110635799

Centisome position: 84.98

GC content: 56.73

Gene sequence:

>1329_bases
GTGCCGGATCAAATACCAGTTCGTGATACAATCTTTGCGCTTTCGAGCGGCTCGTTGCCTTCCGGTATCGCCGTGATACG
GATCTCCGGTGCAGATACGAGAGCTGTCCTGGAACGAATGGCTGGCGGCCTTTCGGAAGCCAGATTCGCCAAGCTGACCA
GCATCTACAGCGCCGAAAGAGAGGTCCTCGACCGAGCACTCTGCCTCTTCTTTCCTGCTCCCGCCAGCTTTACGGGGGAG
GATTGCGCGGAATTGCATTTGCATGGCGGGCGGGCCGTAGTGGCGGCCGTGCTGAACGAGCTTGCCGCATTTCCTGGACT
ACGGATGGCCGAGGCTGGTGAGTTCACGAAACGGGCTTTTCTGAACGGAAAAATGAACCTTACCGAGGCTGAAGCGCTTT
CCGATCTAATTGCTGCAGAAACGGAAGCTCAGAGGCGCTTCGCACTCGTGAACAGCAGTGATATGCGCAAGCGCCTCTAT
GACGGATGGCGCCGCCGCCTCATCCATGCCCGAGCCATGATAGAGGCAGAGCTTGATTTTGCCGATGAATCGGATGTGCC
TGGCTCTGCTGGCGCTACCGTGTGGGAGGACATTCGCCGGCTTCGGCAGGAAATTGTTGAACATGCTTCTTCCTACCGCA
AGGCTGAGATCATCAGGGACGGGTTCCAAATCGTTATCCTGGGCGCACCAAATGCGGGAAAGTCCAGCCTTCTCAATGCG
CTGGCGCGGCGTGACGTAGCCATTGTAACGGAGGAGCCTGGCACGACGCGGGATATTCTCGAGGTCAGCTTGGACATTGA
CGGGACGAAAGTAGTCCTTGCCGACACGGCTGGCATCCGCGAGGCGGCCGGCCGAGTAGAAGCGCTTGGTATCGAACGTT
CTCTGAGGCGTGCCAATGAGGCCGACTTGGTGCTGCTTCTGGAAGACGTCACAAATCCGTCGACACCGGAGGTGCCGCAG
GGCAAGCCGGTGATCACAATAGGGAACAAAGCCGATTTGATCACGGATACACCAGCCGCTTCATACAATTGTTTGATTTC
CGCCAAAACCGGATTGGGACTGGACCGCCTGATGGAGCTGATAAGTCAAGCCAGCGCAGGCTACTCGAATTTCGCAGGGG
AGATCTTGCCCTTTCGCGAGCGGCATGTGGCACTTTTAGGCCAGGCGGCAGACGCGCTTGAGGCTGGAATTGACGGCGAA
AATATTGGGCTTGAGCTGAGAGCTGAATGCCTGCGTTTAAGCTCGCTTGCTTTGGGCCGCATCTCCGGTGAGATCGATGT
TGAAGATTTGCTCGATGCGATCTTCTCCACTTTCTGCATTGGAAAATGA

Upstream 100 bases:

>100_bases
GATAAGGGCTCGCCTGCTCGCCGAGCAGGTTCTTTTCGGGTAAGAGCACATCAATCTCCTATATTGGCCATTTACAACCT
TAGAACCTAGAGCGCTTGCC

Downstream 100 bases:

>100_bases
TTCACGTGAAACATTGGGTCGTCGGCAAAGTGATTCACGTGAAACAGCAATCCATGCGCCGCTGCCGTTTCACGTGAAAC
AGAGCCCGTCTTGACTAAAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MPDQIPVRDTIFALSSGSLPSGIAVIRISGADTRAVLERMAGGLSEARFAKLTSIYSAEREVLDRALCLFFPAPASFTGE
DCAELHLHGGRAVVAAVLNELAAFPGLRMAEAGEFTKRAFLNGKMNLTEAEALSDLIAAETEAQRRFALVNSSDMRKRLY
DGWRRRLIHARAMIEAELDFADESDVPGSAGATVWEDIRRLRQEIVEHASSYRKAEIIRDGFQIVILGAPNAGKSSLLNA
LARRDVAIVTEEPGTTRDILEVSLDIDGTKVVLADTAGIREAAGRVEALGIERSLRRANEADLVLLLEDVTNPSTPEVPQ
GKPVITIGNKADLITDTPAASYNCLISAKTGLGLDRLMELISQASAGYSNFAGEILPFRERHVALLGQAADALEAGIDGE
NIGLELRAECLRLSSLALGRISGEIDVEDLLDAIFSTFCIGK

Sequences:

>Translated_442_residues
MPDQIPVRDTIFALSSGSLPSGIAVIRISGADTRAVLERMAGGLSEARFAKLTSIYSAEREVLDRALCLFFPAPASFTGE
DCAELHLHGGRAVVAAVLNELAAFPGLRMAEAGEFTKRAFLNGKMNLTEAEALSDLIAAETEAQRRFALVNSSDMRKRLY
DGWRRRLIHARAMIEAELDFADESDVPGSAGATVWEDIRRLRQEIVEHASSYRKAEIIRDGFQIVILGAPNAGKSSLLNA
LARRDVAIVTEEPGTTRDILEVSLDIDGTKVVLADTAGIREAAGRVEALGIERSLRRANEADLVLLLEDVTNPSTPEVPQ
GKPVITIGNKADLITDTPAASYNCLISAKTGLGLDRLMELISQASAGYSNFAGEILPFRERHVALLGQAADALEAGIDGE
NIGLELRAECLRLSSLALGRISGEIDVEDLLDAIFSTFCIGK
>Mature_441_residues
PDQIPVRDTIFALSSGSLPSGIAVIRISGADTRAVLERMAGGLSEARFAKLTSIYSAEREVLDRALCLFFPAPASFTGED
CAELHLHGGRAVVAAVLNELAAFPGLRMAEAGEFTKRAFLNGKMNLTEAEALSDLIAAETEAQRRFALVNSSDMRKRLYD
GWRRRLIHARAMIEAELDFADESDVPGSAGATVWEDIRRLRQEIVEHASSYRKAEIIRDGFQIVILGAPNAGKSSLLNAL
ARRDVAIVTEEPGTTRDILEVSLDIDGTKVVLADTAGIREAAGRVEALGIERSLRRANEADLVLLLEDVTNPSTPEVPQG
KPVITIGNKADLITDTPAASYNCLISAKTGLGLDRLMELISQASAGYSNFAGEILPFRERHVALLGQAADALEAGIDGEN
IGLELRAECLRLSSLALGRISGEIDVEDLLDAIFSTFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=460, Percent_Identity=41.7391304347826, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI193082988, Length=492, Percent_Identity=40.0406504065041, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI193082993, Length=443, Percent_Identity=42.4379232505643, Blast_Score=289, Evalue=3e-78,
Organism=Escherichia coli, GI2367268, Length=453, Percent_Identity=39.7350993377483, Blast_Score=270, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI17507259, Length=450, Percent_Identity=33.1111111111111, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=34.8884381338742, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=40.295358649789, Blast_Score=303, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_MESSB (Q11CN2)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_676008.1
- ProteinModelPortal:   Q11CN2
- SMR:   Q11CN2
- STRING:   Q11CN2
- GeneID:   4179880
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_3472
- NMPDR:   fig|266779.1.peg.1061
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q11CN2
- ProtClustDB:   PRK05291
- BioCyc:   MSP266779:MESO_3472-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 47540; Mature: 47408

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 84-84 BINDING 124-124 BINDING 442-442

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDQIPVRDTIFALSSGSLPSGIAVIRISGADTRAVLERMAGGLSEARFAKLTSIYSAER
CCCCCCCHHHEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EVLDRALCLFFPAPASFTGEDCAELHLHGGRAVVAAVLNELAAFPGLRMAEAGEFTKRAF
HHHHHHHHHEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHH
LNGKMNLTEAEALSDLIAAETEAQRRFALVNSSDMRKRLYDGWRRRLIHARAMIEAELDF
HCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADESDVPGSAGATVWEDIRRLRQEIVEHASSYRKAEIIRDGFQIVILGAPNAGKSSLLNA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
LARRDVAIVTEEPGTTRDILEVSLDIDGTKVVLADTAGIREAAGRVEALGIERSLRRANE
HHHCCEEEEECCCCCCHHHEEEEECCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHCCCC
ADLVLLLEDVTNPSTPEVPQGKPVITIGNKADLITDTPAASYNCLISAKTGLGLDRLMEL
CCEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCCHHHHHHH
ISQASAGYSNFAGEILPFRERHVALLGQAADALEAGIDGENIGLELRAECLRLSSLALGR
HHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ISGEIDVEDLLDAIFSTFCIGK
CCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDQIPVRDTIFALSSGSLPSGIAVIRISGADTRAVLERMAGGLSEARFAKLTSIYSAER
CCCCCCHHHEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EVLDRALCLFFPAPASFTGEDCAELHLHGGRAVVAAVLNELAAFPGLRMAEAGEFTKRAF
HHHHHHHHHEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHH
LNGKMNLTEAEALSDLIAAETEAQRRFALVNSSDMRKRLYDGWRRRLIHARAMIEAELDF
HCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ADESDVPGSAGATVWEDIRRLRQEIVEHASSYRKAEIIRDGFQIVILGAPNAGKSSLLNA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
LARRDVAIVTEEPGTTRDILEVSLDIDGTKVVLADTAGIREAAGRVEALGIERSLRRANE
HHHCCEEEEECCCCCCHHHEEEEECCCCCEEEEECCCCHHHHHCCHHHHHHHHHHHCCCC
ADLVLLLEDVTNPSTPEVPQGKPVITIGNKADLITDTPAASYNCLISAKTGLGLDRLMEL
CCEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCCHHHHHHH
ISQASAGYSNFAGEILPFRERHVALLGQAADALEAGIDGENIGLELRAECLRLSSLALGR
HHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
ISGEIDVEDLLDAIFSTFCIGK
CCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA