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The map label for this gene is proB

Identifier: 110635785

GI number: 110635785

Start: 3734704

End: 3735876

Strand: Reverse

Name: proB

Synonym: Meso_3457

Alternate gene names: 110635785

Gene position: 3735876-3734704 (Counterclockwise)

Preceding gene: 110635786

Following gene: 110635784

Centisome position: 84.67

GC content: 64.79

Gene sequence:

>1173_bases
ATGAGTGGAATTTCGCTTTCCTCTTATCGCCGCGTGACCGTGAAGATCGGCTCTGCCCTGCTGGTCGATCGCGCGGCAGG
GTTGAAGCGTGAGTGGCTCGCCGCCCTTGTTGACGATATTGCCCGGCTCATGTCGGCTGGCACGGAAGTGCTCGTTGTAT
CCTCCGGCGCCATTGCCCTGGGACGCACGGTGCTGGGCCTTGGAAAGCGCACATTAAGGCTCGAGGAGAGCCAGGCCGCG
GCGGCCGTGGGGCAGATCGCACTTGCGGGCGCGTGGTCGGGCGAGCTCGGCCGCCACGGGCTCATCTCCGGCCAGGTGCT
GGTTACATTGGGTGATACGGAAGAACGCCGCCGCTACCTCAACGCCCGGGCAACGATCTCGACGCTTCTCAAAATGAAGG
CCGTTCCAGTCATCAATGAGAACGACACCGTGGCGACCAGCGAAATCCGATACGGGGACAACGACCGGTTGGCTGCGCGC
GTCGCCACTATGATGGGCGGCGACCTCCTCATACTCCTGTCCGATGTGGATGGGCTTTACACGGCTCCGCCCGAGCGCGA
TCCTGCGGCAAAGTTCATCCCGGTCGTGGATGCCATCACGCCGGAAATTATGGCTATGGGCGGGGCTGCCGCCTCGGAGC
TATCCCGAGGGGGCATGCGCACCAAGCTCGATGCCGGGCGGATCGCCACCGCGGCGGGTACGGCAATGATCATCGCATCG
GGGAAGGAGCGCGGCCCGCTGTCCGCGATCGAGCGCGGCGCGCGCGCTACCCTCTTCAAGCCGAGCCCCACGCCGGTTCG
CGCCTACAAGACCTGGATCGCGGGTCAACTCGAGCCCGCCGGCCGTATCGGGATCGATGACGGCGCGTTGAGGGCGCTGC
TTCAGGGCAAGAGCCTGCTACCGGCAGGTGTGAGGCGGGTCGAGGGTGTCTTCTCCCGTGGCGACACCGTGGCGATCGTT
GATTCCGCGGGGCGTGAGATTGGCCGGGGGCTGGTTGCCTATGATGCCGCCGATGCCGTAAAGATCGCTGGGCTGAAAAC
GGGCGATATTGCTGGAATCCTGGGTTACGAAGCGCGAGCGGCCATGGTGCACCGGGACGACCTGGTCCTTTCCGGGCCTA
CTGGCGAGCTCATGGATCGCAAGGTGGAAGGAATCGATGTTGCAGGTGCGTGA

Upstream 100 bases:

>100_bases
AGGACGTGCTTCGGGCGCTGATGCGTGTGGTGCAAGCGGCCCGTGAAGAAGAGCGCGCCGCTGAGACCGCAAACGAACGC
TGGCAGAAACAGGCCGACTC

Downstream 100 bases:

>100_bases
GCCGTCGGAGCACGATATCGTTACCCTCATGGCAGAGCTTGGCAGGCGCGGCCGTGCTGCTGCCCGGCCGCTCGCCGTTG
CGAGCACGGAACGGAAAAAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSGISLSSYRRVTVKIGSALLVDRAAGLKREWLAALVDDIARLMSAGTEVLVVSSGAIALGRTVLGLGKRTLRLEESQAA
AAVGQIALAGAWSGELGRHGLISGQVLVTLGDTEERRRYLNARATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAAR
VATMMGGDLLILLSDVDGLYTAPPERDPAAKFIPVVDAITPEIMAMGGAAASELSRGGMRTKLDAGRIATAAGTAMIIAS
GKERGPLSAIERGARATLFKPSPTPVRAYKTWIAGQLEPAGRIGIDDGALRALLQGKSLLPAGVRRVEGVFSRGDTVAIV
DSAGREIGRGLVAYDAADAVKIAGLKTGDIAGILGYEARAAMVHRDDLVLSGPTGELMDRKVEGIDVAGA

Sequences:

>Translated_390_residues
MSGISLSSYRRVTVKIGSALLVDRAAGLKREWLAALVDDIARLMSAGTEVLVVSSGAIALGRTVLGLGKRTLRLEESQAA
AAVGQIALAGAWSGELGRHGLISGQVLVTLGDTEERRRYLNARATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAAR
VATMMGGDLLILLSDVDGLYTAPPERDPAAKFIPVVDAITPEIMAMGGAAASELSRGGMRTKLDAGRIATAAGTAMIIAS
GKERGPLSAIERGARATLFKPSPTPVRAYKTWIAGQLEPAGRIGIDDGALRALLQGKSLLPAGVRRVEGVFSRGDTVAIV
DSAGREIGRGLVAYDAADAVKIAGLKTGDIAGILGYEARAAMVHRDDLVLSGPTGELMDRKVEGIDVAGA
>Mature_389_residues
SGISLSSYRRVTVKIGSALLVDRAAGLKREWLAALVDDIARLMSAGTEVLVVSSGAIALGRTVLGLGKRTLRLEESQAAA
AVGQIALAGAWSGELGRHGLISGQVLVTLGDTEERRRYLNARATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAARV
ATMMGGDLLILLSDVDGLYTAPPERDPAAKFIPVVDAITPEIMAMGGAAASELSRGGMRTKLDAGRIATAAGTAMIIASG
KERGPLSAIERGARATLFKPSPTPVRAYKTWIAGQLEPAGRIGIDDGALRALLQGKSLLPAGVRRVEGVFSRGDTVAIVD
SAGREIGRGLVAYDAADAVKIAGLKTGDIAGILGYEARAAMVHRDDLVLSGPTGELMDRKVEGIDVAGA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=266, Percent_Identity=32.3308270676692, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI21361368, Length=268, Percent_Identity=32.089552238806, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=42.0765027322404, Blast_Score=258, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=252, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17569969, Length=252, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=426, Percent_Identity=30.2816901408451, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=30.2158273381295, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=32.0557491289199, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_MESSB (Q11CP7)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_675993.1
- ProteinModelPortal:   Q11CP7
- SMR:   Q11CP7
- STRING:   Q11CP7
- GeneID:   4181698
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_3457
- NMPDR:   fig|266779.1.peg.1073
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   REPRIAN
- PhylomeDB:   Q11CP7
- ProtClustDB:   PRK05429
- BioCyc:   MSP266779:MESO_3457-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40571; Mature: 40440

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGISLSSYRRVTVKIGSALLVDRAAGLKREWLAALVDDIARLMSAGTEVLVVSSGAIAL
CCCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
GRTVLGLGKRTLRLEESQAAAAVGQIALAGAWSGELGRHGLISGQVLVTLGDTEERRRYL
HHHHHHCCCCEEEECHHHHHHHHHHEEEEECCCCCCCCCCEECCEEEEEECCCHHHHHHH
NARATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAARVATMMGGDLLILLSDVDGLY
HHHHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
TAPPERDPAAKFIPVVDAITPEIMAMGGAAASELSRGGMRTKLDAGRIATAAGTAMIIAS
CCCCCCCCCHHHHHHHHHHCHHHHHCCCCHHHHHHCCCCCEECCCCCEEECCCCEEEEEC
GKERGPLSAIERGARATLFKPSPTPVRAYKTWIAGQLEPAGRIGIDDGALRALLQGKSLL
CCCCCCHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCC
PAGVRRVEGVFSRGDTVAIVDSAGREIGRGLVAYDAADAVKIAGLKTGDIAGILGYEARA
HHHHHHHHHHHCCCCEEEEEECCCHHHHCCEEEECCCCCEEEECCCCCCEEEEECCCHHE
AMVHRDDLVLSGPTGELMDRKVEGIDVAGA
EEEECCCEEEECCCCHHHHCCCCCEECCCC
>Mature Secondary Structure 
SGISLSSYRRVTVKIGSALLVDRAAGLKREWLAALVDDIARLMSAGTEVLVVSSGAIAL
CCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
GRTVLGLGKRTLRLEESQAAAAVGQIALAGAWSGELGRHGLISGQVLVTLGDTEERRRYL
HHHHHHCCCCEEEECHHHHHHHHHHEEEEECCCCCCCCCCEECCEEEEEECCCHHHHHHH
NARATISTLLKMKAVPVINENDTVATSEIRYGDNDRLAARVATMMGGDLLILLSDVDGLY
HHHHHHHHHHHHHCCCEECCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCC
TAPPERDPAAKFIPVVDAITPEIMAMGGAAASELSRGGMRTKLDAGRIATAAGTAMIIAS
CCCCCCCCCHHHHHHHHHHCHHHHHCCCCHHHHHHCCCCCEECCCCCEEECCCCEEEEEC
GKERGPLSAIERGARATLFKPSPTPVRAYKTWIAGQLEPAGRIGIDDGALRALLQGKSLL
CCCCCCHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCC
PAGVRRVEGVFSRGDTVAIVDSAGREIGRGLVAYDAADAVKIAGLKTGDIAGILGYEARA
HHHHHHHHHHHCCCCEEEEEECCCHHHHCCEEEECCCCCEEEECCCCCCEEEEECCCHHE
AMVHRDDLVLSGPTGELMDRKVEGIDVAGA
EEEECCCEEEECCCCHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA