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The map label for this gene is ligT [C]

Identifier: 110635677

GI number: 110635677

Start: 3619224

End: 3619823

Strand: Reverse

Name: ligT [C]

Synonym: Meso_3349

Alternate gene names: 110635677

Gene position: 3619823-3619224 (Counterclockwise)

Preceding gene: 110635678

Following gene: 110635675

Centisome position: 82.04

GC content: 62.5

Gene sequence:

>600_bases
ATGCCGCGACTTTTCACCGCCCTCGAAATTCCGCGCGATGCGGCTTTATCGCTCTCGCTCCTGCGTGGCGGATTGCCGGG
AGCCCGCTGGATCGATGTCGAGAATTATCATCTGACATTGCGCTTCATCGGGGATGTGGAAGGCCATGTGGCCGACGAGA
TCGCCAACGCGCTGGACCGGGTGCGCCGGCCCTCCTTCCTCCTCACTCTTTCCGGCGTTGGCGCATTCGGACAGAAGAAG
CCGCATTCTGTCTGGGCCGGTGTTGCGGCATCGCCTGATCTTCAGGCGCTCCAGGGAGAAATCGAGCGGATCTGCCAACG
CCTGGGCCTGCCGGCCGAGCAACGGAAGTTTACTCCGCATGTGACCTTGGCCCGGCTCAAGAACGCCTCCCCACATGAGG
TCGCTTCCTATCTGTCGGCCCGTGGCAATTTCGCTGCGATGCCCTTCCGGGTAAACCGCTTCGTTTTGATGTCCTCACGG
GATTCCGTAGGCGGCGGGCCCTATGTGGTGGAGGAGGCCTGGCCGCTGTTGCGTACCGAAAGCGCGCGGTCGGCAAGCTT
GTCGGCCAGCGCTTCGGAAGCTTCGCGGATCATGCGGTAA

Upstream 100 bases:

>100_bases
GGACAAGCCGAACATGTGAGGGGAGAGCGGGAGAAACCGCTTGCCCCAACACTCAATCGATGATTCGCTGATGGTGACAT
AGCGAATCGAGGAGGTTGCC

Downstream 100 bases:

>100_bases
ACGGCATCGAAGCCCTCTTCGCCTCCATGATAGGGATCCGGGACCTCTTCGGCTCGATCCAGCGCGTATTGCCGGAAGAG
GTGGATGCGCCCGCAGGGTT

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MPRLFTALEIPRDAALSLSLLRGGLPGARWIDVENYHLTLRFIGDVEGHVADEIANALDRVRRPSFLLTLSGVGAFGQKK
PHSVWAGVAASPDLQALQGEIERICQRLGLPAEQRKFTPHVTLARLKNASPHEVASYLSARGNFAAMPFRVNRFVLMSSR
DSVGGGPYVVEEAWPLLRTESARSASLSASASEASRIMR

Sequences:

>Translated_199_residues
MPRLFTALEIPRDAALSLSLLRGGLPGARWIDVENYHLTLRFIGDVEGHVADEIANALDRVRRPSFLLTLSGVGAFGQKK
PHSVWAGVAASPDLQALQGEIERICQRLGLPAEQRKFTPHVTLARLKNASPHEVASYLSARGNFAAMPFRVNRFVLMSSR
DSVGGGPYVVEEAWPLLRTESARSASLSASASEASRIMR
>Mature_198_residues
PRLFTALEIPRDAALSLSLLRGGLPGARWIDVENYHLTLRFIGDVEGHVADEIANALDRVRRPSFLLTLSGVGAFGQKKP
HSVWAGVAASPDLQALQGEIERICQRLGLPAEQRKFTPHVTLARLKNASPHEVASYLSARGNFAAMPFRVNRFVLMSSRD
SVGGGPYVVEEAWPLLRTESARSASLSASASEASRIMR

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21715; Mature: 21584

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFTALEIPRDAALSLSLLRGGLPGARWIDVENYHLTLRFIGDVEGHVADEIANALDR
CCCCCHHHCCCCHHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCCCCHHHHHHHHHHHH
VRRPSFLLTLSGVGAFGQKKPHSVWAGVAASPDLQALQGEIERICQRLGLPAEQRKFTPH
HCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCC
VTLARLKNASPHEVASYLSARGNFAAMPFRVNRFVLMSSRDSVGGGPYVVEEAWPLLRTE
HHHHHHCCCCHHHHHHHHHCCCCEEECCEEEEEEEEEECCCCCCCCCEEHHCCCCHHHCC
SARSASLSASASEASRIMR
CCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
PRLFTALEIPRDAALSLSLLRGGLPGARWIDVENYHLTLRFIGDVEGHVADEIANALDR
CCCCHHHCCCCHHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCCCCHHHHHHHHHHHH
VRRPSFLLTLSGVGAFGQKKPHSVWAGVAASPDLQALQGEIERICQRLGLPAEQRKFTPH
HCCCCEEEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCC
VTLARLKNASPHEVASYLSARGNFAAMPFRVNRFVLMSSRDSVGGGPYVVEEAWPLLRTE
HHHHHHCCCCHHHHHHHHHCCCCEEECCEEEEEEEEEECCCCCCCCCEEHHCCCCHHHCC
SARSASLSASASEASRIMR
CCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]