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The map label for this gene is fadK [H]

Identifier: 110635281

GI number: 110635281

Start: 3211186

End: 3212811

Strand: Reverse

Name: fadK [H]

Synonym: Meso_2952

Alternate gene names: 110635281

Gene position: 3212811-3211186 (Counterclockwise)

Preceding gene: 110635282

Following gene: 110635280

Centisome position: 72.81

GC content: 59.04

Gene sequence:

>1626_bases
ATGCGACCATTCCTGACACTGCACGATCCCGGACAGGCCAGATCCTTCTATGGAAAGGGCCTGTGGGGCGACGACACGTT
CTACGATTTGATGTGTAGACATGCCGAACATATGCCTTCGGCAAACGCCCTGCGAGATGGCAGCGAGCGCATAGACTGGG
GCACGTTGCGGGCACGGGTCGATGCCCTGGCCGATGATTTTGCCGAGCGTGGCATTGTGGCGGGTGACCGCGTGTCGATC
TGGATGCCGAGCAGCGTGGATGTGGTCGTAGCCTTTCTGGCGTGCTCGCGAATTGGTGCGGCATGCAACCCGTCGCTTCA
TCACAGCTACACCTGCGCGCAGATTATCGATCTGCTGACCATGCTTCAGGCTTCCGCGCTATTGACCGCGGAGGGCTGGG
GCGTGGACCGCGACAGGCACAATTTTGAGGAGATGGCGGCAAAGCTGCCATTTCTGAAGAAGATCTATAAGGCAGGCCGG
CTGCCGATGCACATCACGCACATCTCGGCGGACGCGCACGACAATCCCGACAGTGTCGCTTATCTCGCGTTTACTTCCGG
CTCCACCGGCACGCCCAAATGCGTGATGCACTCATGCAATACGCTGCTGGCCAACGCTCGGAACCTCGTGAGTGATTGGG
GAATATCCAAGAAGGACGTAATCCTGACGCTGAGCCCGCTATCGCATCACATAGGCTGGGTCGCGGCGGCGCAGTGGTTG
CTCAGCGGCGCCGAATTCGTTCTGTATGAGCCGAAGACCATTCCCAACATGCTGAGCTGGATCGAAGAAACCGGCGCGAG
TTATGTGCTGGGTGTACCGACCCACGCGATGGACCTTTTAAGCGAACAGAAGCTCCGTGCACAAAAGAGCATGGGGAAGG
TCCGGATATTTTACATGGCGGGTGCGCCCATTCCTGACGCGATCGCCAGGAGCTTCGCGCAACAGGGCATCAAGCCGCAA
AACATCTACGGCATGACCGAGTGTTCGTCGCATCAGTACACCCATCCCGACGACCCATTGGAGGTGGCGGTCGCGACCTG
TGGCCGTGGCGGAACGGCCTATGAGGTCAAAATTTGGGACCCGCAGGATCCCGACCGTGAGATGCCGGCTGGCGAGACGG
GCGAAATCGGCGGGCGTGGCGCGGCACTGATGCTGGGTTATTACGCCAACCAAGCCGCGACTGAAAAGACGTTCAACCGT
CATGGATATTTGTTGTCGGGCGATCTGGGCTCGTTCGATGCCGACGGCAATTTGCGCGTCGAGGGACGGATCAAGGATCT
GATCATCCGTGGCGGGCATAACATCTTTCCTTCCCGCATAGAGGCTCTGGCCGCAACGCATCCGCGCGTTGAAAAAGTGG
CCGCTTTCCCCGTGCCGGACGAACGTTTGGGTGAAAAGGTCTGCATTGCGGTCATCGGCGAGATAAGCGCGCGAGAACTG
ATGGACCATCTCGCCGCAGAGGGGCTGTCGAAATACGACATGCCCGAATGGTTCATAAGCTTGCCGGCTATGCCGCTGAC
CCCGAGCGGGAAGATACTTAAACGTGAACTGACGGAAATGGTGCGCCGGAGCGAAATTGCGCCGGAGCCCGTCCGATACA
CCACCCCCAAGGTAGCAAGCAGATGA

Upstream 100 bases:

>100_bases
TCCCAATATCTGGGCACGGAAGAACAGAAGGAGCTTTTCGCAAGCTTCAATGAACGGCGTGAGCCCGATAGCTCCAAATT
CGGCCATTGACGAGAGCGGC

Downstream 100 bases:

>100_bases
CACTCACATTGGTTCGAAAGGAAGAGTTTGCGCTTCTTACGCTGAACCGCCCAGAGGCTTTGAATGCATTGTCCTTCGCG
CTTTTGAAGGATATCGCCGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MRPFLTLHDPGQARSFYGKGLWGDDTFYDLMCRHAEHMPSANALRDGSERIDWGTLRARVDALADDFAERGIVAGDRVSI
WMPSSVDVVVAFLACSRIGAACNPSLHHSYTCAQIIDLLTMLQASALLTAEGWGVDRDRHNFEEMAAKLPFLKKIYKAGR
LPMHITHISADAHDNPDSVAYLAFTSGSTGTPKCVMHSCNTLLANARNLVSDWGISKKDVILTLSPLSHHIGWVAAAQWL
LSGAEFVLYEPKTIPNMLSWIEETGASYVLGVPTHAMDLLSEQKLRAQKSMGKVRIFYMAGAPIPDAIARSFAQQGIKPQ
NIYGMTECSSHQYTHPDDPLEVAVATCGRGGTAYEVKIWDPQDPDREMPAGETGEIGGRGAALMLGYYANQAATEKTFNR
HGYLLSGDLGSFDADGNLRVEGRIKDLIIRGGHNIFPSRIEALAATHPRVEKVAAFPVPDERLGEKVCIAVIGEISAREL
MDHLAAEGLSKYDMPEWFISLPAMPLTPSGKILKRELTEMVRRSEIAPEPVRYTTPKVASR

Sequences:

>Translated_541_residues
MRPFLTLHDPGQARSFYGKGLWGDDTFYDLMCRHAEHMPSANALRDGSERIDWGTLRARVDALADDFAERGIVAGDRVSI
WMPSSVDVVVAFLACSRIGAACNPSLHHSYTCAQIIDLLTMLQASALLTAEGWGVDRDRHNFEEMAAKLPFLKKIYKAGR
LPMHITHISADAHDNPDSVAYLAFTSGSTGTPKCVMHSCNTLLANARNLVSDWGISKKDVILTLSPLSHHIGWVAAAQWL
LSGAEFVLYEPKTIPNMLSWIEETGASYVLGVPTHAMDLLSEQKLRAQKSMGKVRIFYMAGAPIPDAIARSFAQQGIKPQ
NIYGMTECSSHQYTHPDDPLEVAVATCGRGGTAYEVKIWDPQDPDREMPAGETGEIGGRGAALMLGYYANQAATEKTFNR
HGYLLSGDLGSFDADGNLRVEGRIKDLIIRGGHNIFPSRIEALAATHPRVEKVAAFPVPDERLGEKVCIAVIGEISAREL
MDHLAAEGLSKYDMPEWFISLPAMPLTPSGKILKRELTEMVRRSEIAPEPVRYTTPKVASR
>Mature_541_residues
MRPFLTLHDPGQARSFYGKGLWGDDTFYDLMCRHAEHMPSANALRDGSERIDWGTLRARVDALADDFAERGIVAGDRVSI
WMPSSVDVVVAFLACSRIGAACNPSLHHSYTCAQIIDLLTMLQASALLTAEGWGVDRDRHNFEEMAAKLPFLKKIYKAGR
LPMHITHISADAHDNPDSVAYLAFTSGSTGTPKCVMHSCNTLLANARNLVSDWGISKKDVILTLSPLSHHIGWVAAAQWL
LSGAEFVLYEPKTIPNMLSWIEETGASYVLGVPTHAMDLLSEQKLRAQKSMGKVRIFYMAGAPIPDAIARSFAQQGIKPQ
NIYGMTECSSHQYTHPDDPLEVAVATCGRGGTAYEVKIWDPQDPDREMPAGETGEIGGRGAALMLGYYANQAATEKTFNR
HGYLLSGDLGSFDADGNLRVEGRIKDLIIRGGHNIFPSRIEALAATHPRVEKVAAFPVPDERLGEKVCIAVIGEISAREL
MDHLAAEGLSKYDMPEWFISLPAMPLTPSGKILKRELTEMVRRSEIAPEPVRYTTPKVASR

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It has a low activity on medium or long chain fatty acids and is ma

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein. Note=Partially membrane-associated [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=540, Percent_Identity=26.2962962962963, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI157311624, Length=486, Percent_Identity=25.3086419753086, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=486, Percent_Identity=25.3086419753086, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI58082049, Length=486, Percent_Identity=25.3086419753086, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI122937307, Length=485, Percent_Identity=24.1237113402062, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI187761345, Length=534, Percent_Identity=26.2172284644195, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI187761343, Length=534, Percent_Identity=26.2172284644195, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI42544132, Length=480, Percent_Identity=24.5833333333333, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI38505220, Length=489, Percent_Identity=24.9488752556237, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI8923896, Length=550, Percent_Identity=22.9090909090909, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI145693145, Length=542, Percent_Identity=29.520295202952, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1786810, Length=523, Percent_Identity=25.6214149139579, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788107, Length=542, Percent_Identity=26.0147601476015, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788595, Length=500, Percent_Identity=26, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI221142682, Length=503, Percent_Identity=23.6580516898608, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786801, Length=447, Percent_Identity=24.6085011185682, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789201, Length=478, Percent_Identity=24.4769874476987, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71994694, Length=523, Percent_Identity=25.2390057361377, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=523, Percent_Identity=25.2390057361377, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=523, Percent_Identity=25.2390057361377, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI32563687, Length=499, Percent_Identity=25.8517034068136, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=493, Percent_Identity=26.1663286004057, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=517, Percent_Identity=25.9187620889749, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=501, Percent_Identity=26.7465069860279, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=499, Percent_Identity=26.25250501002, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=378, Percent_Identity=27.2486772486773, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=371, Percent_Identity=24.7978436657682, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=361, Percent_Identity=23.2686980609418, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=608, Percent_Identity=21.2171052631579, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=555, Percent_Identity=23.2432432432432, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=555, Percent_Identity=23.2432432432432, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=485, Percent_Identity=26.5979381443299, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6319264, Length=594, Percent_Identity=22.5589225589226, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581924, Length=533, Percent_Identity=26.6416510318949, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21355181, Length=498, Percent_Identity=25.3012048192771, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18859661, Length=366, Percent_Identity=27.5956284153005, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922652, Length=504, Percent_Identity=23.6111111111111, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648260, Length=349, Percent_Identity=25.214899713467, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24656500, Length=513, Percent_Identity=24.1715399610136, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20130357, Length=498, Percent_Identity=24.0963855421687, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356947, Length=320, Percent_Identity=25, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921316, Length=160, Percent_Identity=34.375, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648253, Length=461, Percent_Identity=22.1258134490239, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648255, Length=466, Percent_Identity=21.8884120171674, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648257, Length=395, Percent_Identity=25.8227848101266, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358303, Length=517, Percent_Identity=20.5029013539652, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356441, Length=505, Percent_Identity=19.8019801980198, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653035, Length=317, Percent_Identity=23.9747634069401, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 59400; Mature: 59400

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFLTLHDPGQARSFYGKGLWGDDTFYDLMCRHAEHMPSANALRDGSERIDWGTLRARV
CCCCEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHH
DALADDFAERGIVAGDRVSIWMPSSVDVVVAFLACSRIGAACNPSLHHSYTCAQIIDLLT
HHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
MLQASALLTAEGWGVDRDRHNFEEMAAKLPFLKKIYKAGRLPMHITHISADAHDNPDSVA
HHHHHHHEEECCCCCCCHHCCHHHHHHHCHHHHHHHHCCCCCEEEEEEECCCCCCCCCEE
YLAFTSGSTGTPKCVMHSCNTLLANARNLVSDWGISKKDVILTLSPLSHHIGWVAAAQWL
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
LSGAEFVLYEPKTIPNMLSWIEETGASYVLGVPTHAMDLLSEQKLRAQKSMGKVRIFYMA
HCCCCEEEECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEE
GAPIPDAIARSFAQQGIKPQNIYGMTECSSHQYTHPDDPLEVAVATCGRGGTAYEVKIWD
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEEEEEC
PQDPDREMPAGETGEIGGRGAALMLGYYANQAATEKTFNRHGYLLSGDLGSFDADGNLRV
CCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEE
EGRIKDLIIRGGHNIFPSRIEALAATHPRVEKVAAFPVPDERLGEKVCIAVIGEISAREL
ECEEEEEEEECCCCCCHHHHHHHHHCCCCHHHEEECCCCHHHHCHHEEHHHHHCHHHHHH
MDHLAAEGLSKYDMPEWFISLPAMPLTPSGKILKRELTEMVRRSEIAPEPVRYTTPKVAS
HHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCC
R
C
>Mature Secondary Structure
MRPFLTLHDPGQARSFYGKGLWGDDTFYDLMCRHAEHMPSANALRDGSERIDWGTLRARV
CCCCEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHH
DALADDFAERGIVAGDRVSIWMPSSVDVVVAFLACSRIGAACNPSLHHSYTCAQIIDLLT
HHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
MLQASALLTAEGWGVDRDRHNFEEMAAKLPFLKKIYKAGRLPMHITHISADAHDNPDSVA
HHHHHHHEEECCCCCCCHHCCHHHHHHHCHHHHHHHHCCCCCEEEEEEECCCCCCCCCEE
YLAFTSGSTGTPKCVMHSCNTLLANARNLVSDWGISKKDVILTLSPLSHHIGWVAAAQWL
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHH
LSGAEFVLYEPKTIPNMLSWIEETGASYVLGVPTHAMDLLSEQKLRAQKSMGKVRIFYMA
HCCCCEEEECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEE
GAPIPDAIARSFAQQGIKPQNIYGMTECSSHQYTHPDDPLEVAVATCGRGGTAYEVKIWD
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCEEEEEEEC
PQDPDREMPAGETGEIGGRGAALMLGYYANQAATEKTFNRHGYLLSGDLGSFDADGNLRV
CCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEE
EGRIKDLIIRGGHNIFPSRIEALAATHPRVEKVAAFPVPDERLGEKVCIAVIGEISAREL
ECEEEEEEEECCCCCCHHHHHHHHHCCCCHHHEEECCCCHHHHCHHEEHHHHHCHHHHHH
MDHLAAEGLSKYDMPEWFISLPAMPLTPSGKILKRELTEMVRRSEIAPEPVRYTTPKVAS
HHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 7984428; 12535077 [H]