BacMap

Definition	Mesorhizobium sp. BNC1, complete genome.
Accession	NC_008254
Length	4,412,446

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The map label for this gene is paaF [C]

Identifier: 110635280

GI number: 110635280

Start: 3210416

End: 3211189

Strand: Reverse

Name: paaF [C]

Synonym: Meso_2951

Alternate gene names: 110635280

Gene position: 3211189-3210416 (Counterclockwise)

Preceding gene: 110635281

Following gene: 110635279

Centisome position: 72.78

GC content: 61.63

Gene sequence:

>774_bases
ATGACACTCACATTGGTTCGAAAGGAAGAGTTTGCGCTTCTTACGCTGAACCGCCCAGAGGCTTTGAATGCATTGTCCTT
CGCGCTTTTGAAGGATATCGCCGATGCGCTTGACGAAGTGGCCGGTTGGCGAGATGTGCGCGCGCTCTTCATAACGGGGG
CTGGCCAGAAGGCGTTTTGCGCCGGCGCCGACATTAAGGAATTGCGTCATCGTAGCCTGTCGGAGCAGAAACGCGGCGCT
GAAGCCGGCCAGGCGACCTTCGCCCGGCTCGACCGTTTGCCGATCGCCTCGGTGGCACTGATCAATGGCTATGCCTTCGG
CGGCGGGCTTGAATTGGCCCTGGCGGCAACTTTCCGCATTGCAAGCTCCAACGCGCTGTTCGGCCTGCCGGAGGTCAAAT
TAGGGCTCATCCCCGGCTATGGCGGAACGCAACGCCTACCACGCATCGTGGGCGAGGCGCGGGCTCTCGAGATGATCATG
ACCGGGCGCTCCGTGGCGGCTGAAGAGGCCGAGCGTATTGGGCTGATACACCAAGTCGTAAACGACGGGGACCTTTGGGA
AGCCGGTGTCGCCTTCGCCCGCCGCTTCACGCGGTTCAGCTTGCCCTCTCTGGAACTTGCTCGCCGGGCGGTGCAGCGTG
CCGCAGAAATGCCGCTTGCCGACGGGCTTCAGATGGAAGCGGAGCTGTCCACGCTGGCATATCGCACCGCTGACGCGGAG
GAAGGCATGGCGGCTTTCGAGGAAAAGCGCCAGGCGGAGTTTAAGGATGAATAA

Upstream 100 bases:

>100_bases
CGAGCGGGAAGATACTTAAACGTGAACTGACGGAAATGGTGCGCCGGAGCGAAATTGCGCCGGAGCCCGTCCGATACACC
ACCCCCAAGGTAGCAAGCAG

Downstream 100 bases:

>100_bases
TCGGCAGATCATCGTCACCGGCGCATCCAAGGGGATCGGCCTTTCCGTCGCCTCTGAGCTCGCGCGGCGTGGTTACCTGC
CGGTCTGCCTTTCGCGCTCA

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTLTLVRKEEFALLTLNRPEALNALSFALLKDIADALDEVAGWRDVRALFITGAGQKAFCAGADIKELRHRSLSEQKRGA
EAGQATFARLDRLPIASVALINGYAFGGGLELALAATFRIASSNALFGLPEVKLGLIPGYGGTQRLPRIVGEARALEMIM
TGRSVAAEEAERIGLIHQVVNDGDLWEAGVAFARRFTRFSLPSLELARRAVQRAAEMPLADGLQMEAELSTLAYRTADAE
EGMAAFEEKRQAEFKDE

Sequences:

>Translated_257_residues
MTLTLVRKEEFALLTLNRPEALNALSFALLKDIADALDEVAGWRDVRALFITGAGQKAFCAGADIKELRHRSLSEQKRGA
EAGQATFARLDRLPIASVALINGYAFGGGLELALAATFRIASSNALFGLPEVKLGLIPGYGGTQRLPRIVGEARALEMIM
TGRSVAAEEAERIGLIHQVVNDGDLWEAGVAFARRFTRFSLPSLELARRAVQRAAEMPLADGLQMEAELSTLAYRTADAE
EGMAAFEEKRQAEFKDE
>Mature_256_residues
TLTLVRKEEFALLTLNRPEALNALSFALLKDIADALDEVAGWRDVRALFITGAGQKAFCAGADIKELRHRSLSEQKRGAE
AGQATFARLDRLPIASVALINGYAFGGGLELALAATFRIASSNALFGLPEVKLGLIPGYGGTQRLPRIVGEARALEMIMT
GRSVAAEEAERIGLIHQVVNDGDLWEAGVAFARRFTRFSLPSLELARRAVQRAAEMPLADGLQMEAELSTLAYRTADAEE
GMAAFEEKRQAEFKDE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=39.0438247011952, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=37.4501992031873, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI68989263, Length=207, Percent_Identity=40.0966183574879, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=36.7231638418079, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI150378541, Length=245, Percent_Identity=34.6938775510204, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI213417737, Length=232, Percent_Identity=28.448275862069, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI157694516, Length=232, Percent_Identity=28.448275862069, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=29.9212598425197, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI261878539, Length=113, Percent_Identity=46.9026548672566, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI37594469, Length=181, Percent_Identity=33.1491712707182, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI37594471, Length=181, Percent_Identity=33.1491712707182, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=30.1075268817204, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI157694520, Length=207, Percent_Identity=27.0531400966184, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI25453481, Length=259, Percent_Identity=26.6409266409266, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI51538573, Length=259, Percent_Identity=26.6409266409266, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4757968, Length=259, Percent_Identity=25.8687258687259, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI49355787, Length=259, Percent_Identity=25.8687258687259, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI4757966, Length=248, Percent_Identity=26.6129032258064, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI51538581, Length=248, Percent_Identity=26.6129032258064, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136756, Length=260, Percent_Identity=26.1538461538462, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI221136753, Length=260, Percent_Identity=26.1538461538462, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI62530384, Length=218, Percent_Identity=25.6880733944954, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI221307494, Length=259, Percent_Identity=25.0965250965251, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=38.3399209486166, Blast_Score=160, Evalue=6e-41,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=35.4961832061069, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=37.8109452736318, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1790281, Length=188, Percent_Identity=35.6382978723404, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787660, Length=248, Percent_Identity=35.8870967741936, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788597, Length=247, Percent_Identity=31.9838056680162, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=25, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=36.8, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=37.109375, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=34.2342342342342, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=34.2541436464088, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=263, Percent_Identity=27.7566539923954, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=178, Percent_Identity=33.1460674157303, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17508951, Length=178, Percent_Identity=33.1460674157303, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508953, Length=178, Percent_Identity=33.1460674157303, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=185, Percent_Identity=31.8918918918919, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=223, Percent_Identity=30.0448430493274, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=223, Percent_Identity=30.0448430493274, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=179, Percent_Identity=29.0502793296089, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=27.9166666666667, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=243, Percent_Identity=25.5144032921811, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=174, Percent_Identity=29.8850574712644, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=24.21875, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=186, Percent_Identity=31.1827956989247, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=40.9638554216867, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=40.9638554216867, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=33.4645669291339, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28571729, Length=141, Percent_Identity=34.7517730496454, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571730, Length=141, Percent_Identity=34.7517730496454, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24650670, Length=259, Percent_Identity=29.7297297297297, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922422, Length=244, Percent_Identity=26.2295081967213, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920382, Length=197, Percent_Identity=29.4416243654822, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21357171, Length=212, Percent_Identity=30.188679245283, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583165, Length=259, Percent_Identity=27.7992277992278, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921000, Length=137, Percent_Identity=43.7956204379562, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583077, Length=137, Percent_Identity=43.7956204379562, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583079, Length=137, Percent_Identity=43.7956204379562, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921018, Length=182, Percent_Identity=32.967032967033, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27893; Mature: 27762

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTLVRKEEFALLTLNRPEALNALSFALLKDIADALDEVAGWRDVRALFITGAGQKAFC
CEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCEEE
AGADIKELRHRSLSEQKRGAEAGQATFARLDRLPIASVALINGYAFGGGLELALAATFRI
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCEECCCCHHHHHHHHHHE
ASSNALFGLPEVKLGLIPGYGGTQRLPRIVGEARALEMIMTGRSVAAEEAERIGLIHQVV
ECCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
NDGDLWEAGVAFARRFTRFSLPSLELARRAVQRAAEMPLADGLQMEAELSTLAYRTADAE
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHH
EGMAAFEEKRQAEFKDE
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLTLVRKEEFALLTLNRPEALNALSFALLKDIADALDEVAGWRDVRALFITGAGQKAFC
EEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCEEE
AGADIKELRHRSLSEQKRGAEAGQATFARLDRLPIASVALINGYAFGGGLELALAATFRI
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCEECCCCHHHHHHHHHHE
ASSNALFGLPEVKLGLIPGYGGTQRLPRIVGEARALEMIMTGRSVAAEEAERIGLIHQVV
ECCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
NDGDLWEAGVAFARRFTRFSLPSLELARRAVQRAAEMPLADGLQMEAELSTLAYRTADAE
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHH
EGMAAFEEKRQAEFKDE
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA