Click here to switch to the map view.

The map label for this gene is rffG [C]

Identifier: 110635107

GI number: 110635107

Start: 3021585

End: 3022577

Strand: Reverse

Name: rffG [C]

Synonym: Meso_2774

Alternate gene names: 110635107

Gene position: 3022577-3021585 (Counterclockwise)

Preceding gene: 110635108

Following gene: 110635106

Centisome position: 68.5

GC content: 59.52

Gene sequence:

>993_bases
ATGGATGTCGTAAGAGGGAAGGCGAGGCGAAAGCGCGTGCTGGTGGCAGGCGGAGCCGGCTTTGTGGGGTCGCATCTTTG
CGATGCGCTGCTGGCCGAGGGCTGCAAGGTCGTATGCGTCGACAGTTTCCTGACGGGAGCCTATGCCAATATCGCTCCGC
TGGAAAACCATGGGAGATTTCAGCTTATTCGGCAGGATATCTGTAAGCCGCTCAAGCTGGAGGACAAGCTGGATGAGATC
TACAATCTCGCATGTGCGGCCTCTCCGCCGATCTACCAGATGGACCCTGTCCATACCCTGATGACAAGCGTGCTGGGCAC
GCGGAACCTGCTTGCGCTGGCGGAAAAACACGGTGCCCGCTTCCTGCAGGCCTCCACAAGCGAGGTCTACGGCGACCCCG
AAGTCCATCCGCAGCCGGAAGATTACCGCGGCAATGTCAACCCGACAGGACCGCGGGCCTGCTATGACGAGGGAAAACGC
GCTGCAGAGGCACTCTGCTTCGATCTTCTTCGCCTCGGGCGGGTGGATGCGCGCGTGGCGCGGATTTTCAACACCTATGG
ACCGCGCATGAAGGCCGATGACGGCCGTATTGTCTCAAATTTCATCAACCAGGCGCTTCGGGGTGAGCCGCTTACAATCT
ACGGAACGGGCGAGCAGACACGCTCTTTCTGCCATGTATCGGATCTCGTCAGAGGCCTTGTCGCCTTGATGGAAGTGCAG
CCCAACCCGAAAATGCCGATCAATCTCGGCAATCCCGGAGAATTCACCGTCAATCAGCTCGCCGCGATCATCAGCGAGAG
CGTTCCGGGAGCGAAAGGCGTGATCCATCGTCCGCTGCCGCAGGACGATCCCCAGCGCCGGCAGCCAGATATTAGACGCG
CGGAGAAATTGCTGAATTGGTCTCCCCGAATTGCGCTGCGGGAAGGTCTGGAGGATACGATTGCTTGGTTTCGGCTCGCC
GACGAAGCGGAGAAACGCACTACGGCTGCTTGA

Upstream 100 bases:

>100_bases
TCAGGGCGCGGCAACTCGCGGGCATTCTTCGAGAAGCGGGCAACGCTGTGCTGGCGAAGGCCGTTTAACGCATCGACCAT
CGGGTCAGAAGGAACTCTGA

Downstream 100 bases:

>100_bases
CCCAATCCGGCGCGGACACTTCCTTGCGAGCAGCGGCAGCCTCCTCCCGGCTCCGCTTTTCTTGAAGCATTTTCTGGAGC
TTTATCGCCAAGGTGAGCAG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MDVVRGKARRKRVLVAGGAGFVGSHLCDALLAEGCKVVCVDSFLTGAYANIAPLENHGRFQLIRQDICKPLKLEDKLDEI
YNLACAASPPIYQMDPVHTLMTSVLGTRNLLALAEKHGARFLQASTSEVYGDPEVHPQPEDYRGNVNPTGPRACYDEGKR
AAEALCFDLLRLGRVDARVARIFNTYGPRMKADDGRIVSNFINQALRGEPLTIYGTGEQTRSFCHVSDLVRGLVALMEVQ
PNPKMPINLGNPGEFTVNQLAAIISESVPGAKGVIHRPLPQDDPQRRQPDIRRAEKLLNWSPRIALREGLEDTIAWFRLA
DEAEKRTTAA

Sequences:

>Translated_330_residues
MDVVRGKARRKRVLVAGGAGFVGSHLCDALLAEGCKVVCVDSFLTGAYANIAPLENHGRFQLIRQDICKPLKLEDKLDEI
YNLACAASPPIYQMDPVHTLMTSVLGTRNLLALAEKHGARFLQASTSEVYGDPEVHPQPEDYRGNVNPTGPRACYDEGKR
AAEALCFDLLRLGRVDARVARIFNTYGPRMKADDGRIVSNFINQALRGEPLTIYGTGEQTRSFCHVSDLVRGLVALMEVQ
PNPKMPINLGNPGEFTVNQLAAIISESVPGAKGVIHRPLPQDDPQRRQPDIRRAEKLLNWSPRIALREGLEDTIAWFRLA
DEAEKRTTAA
>Mature_330_residues
MDVVRGKARRKRVLVAGGAGFVGSHLCDALLAEGCKVVCVDSFLTGAYANIAPLENHGRFQLIRQDICKPLKLEDKLDEI
YNLACAASPPIYQMDPVHTLMTSVLGTRNLLALAEKHGARFLQASTSEVYGDPEVHPQPEDYRGNVNPTGPRACYDEGKR
AAEALCFDLLRLGRVDARVARIFNTYGPRMKADDGRIVSNFINQALRGEPLTIYGTGEQTRSFCHVSDLVRGLVALMEVQ
PNPKMPINLGNPGEFTVNQLAAIISESVPGAKGVIHRPLPQDDPQRRQPDIRRAEKLLNWSPRIALREGLEDTIAWFRLA
DEAEKRTTAA

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=309, Percent_Identity=55.3398058252427, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI7657641, Length=326, Percent_Identity=26.9938650306748, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI48994969, Length=342, Percent_Identity=28.3625730994152, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788589, Length=351, Percent_Identity=26.4957264957265, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788353, Length=342, Percent_Identity=24.8538011695906, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786974, Length=334, Percent_Identity=28.1437125748503, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=323, Percent_Identity=50.4643962848297, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI115532424, Length=326, Percent_Identity=28.8343558282209, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=347, Percent_Identity=26.5129682997118, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=24.251497005988, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=355, Percent_Identity=25.9154929577465, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356223, Length=309, Percent_Identity=55.3398058252427, Blast_Score=354, Evalue=4e-98,
Organism=Drosophila melanogaster, GI19923002, Length=343, Percent_Identity=25.3644314868805, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 36390; Mature: 36390

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVVRGKARRKRVLVAGGAGFVGSHLCDALLAEGCKVVCVDSFLTGAYANIAPLENHGRF
CCCCCCCHHHCEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCCCCCHH
QLIRQDICKPLKLEDKLDEIYNLACAASPPIYQMDPVHTLMTSVLGTRNLLALAEKHGAR
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHH
FLQASTSEVYGDPEVHPQPEDYRGNVNPTGPRACYDEGKRAAEALCFDLLRLGRVDARVA
HHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
RIFNTYGPRMKADDGRIVSNFINQALRGEPLTIYGTGEQTRSFCHVSDLVRGLVALMEVQ
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
PNPKMPINLGNPGEFTVNQLAAIISESVPGAKGVIHRPLPQDDPQRRQPDIRRAEKLLNW
CCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHCCCHHHHHHHHHCC
SPRIALREGLEDTIAWFRLADEAEKRTTAA
CCCHHHHHCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVVRGKARRKRVLVAGGAGFVGSHLCDALLAEGCKVVCVDSFLTGAYANIAPLENHGRF
CCCCCCCHHHCEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCCCCCHH
QLIRQDICKPLKLEDKLDEIYNLACAASPPIYQMDPVHTLMTSVLGTRNLLALAEKHGAR
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHH
FLQASTSEVYGDPEVHPQPEDYRGNVNPTGPRACYDEGKRAAEALCFDLLRLGRVDARVA
HHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
RIFNTYGPRMKADDGRIVSNFINQALRGEPLTIYGTGEQTRSFCHVSDLVRGLVALMEVQ
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
PNPKMPINLGNPGEFTVNQLAAIISESVPGAKGVIHRPLPQDDPQRRQPDIRRAEKLLNW
CCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCHHCCCHHHHHHHHHCC
SPRIALREGLEDTIAWFRLADEAEKRTTAA
CCCHHHHHCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]