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The map label for this gene is yfkN [H]

Identifier: 110635059

GI number: 110635059

Start: 2962518

End: 2964509

Strand: Reverse

Name: yfkN [H]

Synonym: Meso_2725

Alternate gene names: 110635059

Gene position: 2964509-2962518 (Counterclockwise)

Preceding gene: 110635060

Following gene: 110635058

Centisome position: 67.19

GC content: 62.85

Gene sequence:

>1992_bases
ATGAAAAACTTGGCAGTCCTTGCCCTCGGTGCTTCGGCTTTGGCGCTTTCTTCGGCACCTTCCTTCGCCGACTACACGCT
TAACATCCTGCACATCAACGACTGGCACAGCCGCATCCAATCCATCAACGCGTTCGAGTCCACCTGTTCGGATGAGGACG
AATCGGCGGGCAAATGCTTCGGCGGCGCAGGCCGGCTTGTGACTGCCGTGGAGGATGCGCGCAACGCGCTCCAGGGACAG
AACGTGCTTTTCCTGAACGCCGGCGACAATTTCCAGGGTTCGCTTTTCTACATCACCTATAAGGGAACCGCTGAGGCCGA
ATTTCTGAACGTCATGAAGACCGACGCCATGGTGGTCGGCAATCACGAGTTCGATGACGGCGAGGATGGCCTTGCCGCCT
TCCTCGAGAAGGTGGAGTTCCCCGTGCTCGGCTCCAATGTGAAGGCGCAGCAGGCGTCCAAGCTGGGCGACCGAGTGAAG
GAATATGTGATCCTCGATGTGGGCGGCGAGAAGATCGGCATTGTCGGGGCCGTGGCCAACGACACGGCCGAGCTCTCCTC
GCCCGGGGAAAACGTCTCCATCATCACTGATGTCGATGGCATCACCGCCGCGGTGAAGGCGGTGCAGGACGAGGGCGTGA
ACAAGATCATAGCGCTCACCCATGTGGGCTACCCGCGTGATCTTGAAGCCATAGCAAAAATTCCCGGCGTGGATGTGGTG
GTGGGCGGCCACAGCCACACGTTGCTTTCCAACACCGCGGAGGGCGCCGAGGGCCCATATCCGACCATGGTGGAGAACCC
CGAGGGCTATCAGGTGCCTGTCGTTCAGGCGGCTTCCTATTCGAAATATCTGGGCGACCTGAAAGTGGTGTTCGACGATT
CAGGCGTGGTCAAGGAAGCTTCCGGCGAGCCGAAGCTGCTCGATGCCCAGGTCCAGCCGGACGAGGCGGTGCTGGCGCGC
GTGAAAGAGCTCGCCGGTCCAATCGAGGAGCTGAAGGCCCAGGTGGTCTCCGAAACCTCCGCGTCAATTGACGGCAGCCG
CGAAACCTGCCGCGCCCGCGAATGTCAGATGGGCAATCTGGTGACGGATGCCATGCTGGCCCGCACCAAGGACCAGGGTG
TGCAATTCGCCATCACCAATGGCGGCGGCCTGCGCGCCTCGATCGATGCGGGCGAAGTCACGATGGGCGAGGTGCTGGAG
GTGCTGCCCTTCCAGAACACGCTCGCGACCTTCCAACTCACCGGTTCGGATGTGATCGCTTCGCTCGAAAGCGGCGTCAG
CCAGATCGAGGAAGGGGCCGGCCGTTTTCCCCAGGTGTCCGGCCTGCGCTACAGCTTCGACAAATCGGCGCCTGCCGGCC
AGGGCCGTATCAGCAATGTAGAGGTGCAGACGGCAGATGGCGGCTGGGCGCCCATCGACCCGAACGCTACCTACACCATC
GCCACCAACAATTTCATGCGCAATGGCGGCGATGGCTACAGCCTTTTTGGGGAAAAGGGCCAGAACGCTTATGATTACGG
CCCCGGCCTTGAGCAGGTGCTGGCCGATTATCTCGCGGCCAACAATCCCTACAAGCCCTATACCGATGGCCGCATAACGG
AGGTGGCCGCCGCCGCGCCTTCGGATTCTGCGGAGCCTCCATCTGCCGAAGCTCCCGCCACGCAGCCGTCGGGCGAGGCC
TCGACCACAACCCAGCCTGTTCAGCCGGCGCCTGCGCCGGGCGAAACAGCCGAAGCCACGCCGCCGGCCGGTGAGGCCGA
ACCGGCGGAGTCCGGCACGGAGACGGCCGCGGCGGCCCCGGATGCTGGCGCGGCCGAAGCCAGGGAGGCTGTCACCACGC
ATGTTATCGTCAGCGGCGACACGTTGTGGAACCTTGCGGAGGAATTCTACGGCGATCCAATGCAGTGGCGAAAGCTTGCG
GACGCAAATCCGCAATTGCGGCCCAACCGGCTAGTCGTGGGCGCAACCCTCAACATTCCGCCGGCGGAATAG

Upstream 100 bases:

>100_bases
CGAAGCCGGTGCCAACATGCCGGCAGGATATGCAGCTCGTTGCGAAACCGACACGATAGCGCGCTAGAGCGGGAACACGG
CAGTCTCGGGGAGATCAGAC

Downstream 100 bases:

>100_bases
CCGAGCTTCAGGGGATTGCCTGAACGCCGAAGCGTAAATGCCGCGCGCCGCAGCGGCATTTTCCCTCCTGCGGCTAGAAG
TGGCATTGCTTTCGCGCCGA

Product: 5'-nucleotidase-like

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 663; Mature: 663

Protein sequence:

>663_residues
MKNLAVLALGASALALSSAPSFADYTLNILHINDWHSRIQSINAFESTCSDEDESAGKCFGGAGRLVTAVEDARNALQGQ
NVLFLNAGDNFQGSLFYITYKGTAEAEFLNVMKTDAMVVGNHEFDDGEDGLAAFLEKVEFPVLGSNVKAQQASKLGDRVK
EYVILDVGGEKIGIVGAVANDTAELSSPGENVSIITDVDGITAAVKAVQDEGVNKIIALTHVGYPRDLEAIAKIPGVDVV
VGGHSHTLLSNTAEGAEGPYPTMVENPEGYQVPVVQAASYSKYLGDLKVVFDDSGVVKEASGEPKLLDAQVQPDEAVLAR
VKELAGPIEELKAQVVSETSASIDGSRETCRARECQMGNLVTDAMLARTKDQGVQFAITNGGGLRASIDAGEVTMGEVLE
VLPFQNTLATFQLTGSDVIASLESGVSQIEEGAGRFPQVSGLRYSFDKSAPAGQGRISNVEVQTADGGWAPIDPNATYTI
ATNNFMRNGGDGYSLFGEKGQNAYDYGPGLEQVLADYLAANNPYKPYTDGRITEVAAAAPSDSAEPPSAEAPATQPSGEA
STTTQPVQPAPAPGETAEATPPAGEAEPAESGTETAAAAPDAGAAEAREAVTTHVIVSGDTLWNLAEEFYGDPMQWRKLA
DANPQLRPNRLVVGATLNIPPAE

Sequences:

>Translated_663_residues
MKNLAVLALGASALALSSAPSFADYTLNILHINDWHSRIQSINAFESTCSDEDESAGKCFGGAGRLVTAVEDARNALQGQ
NVLFLNAGDNFQGSLFYITYKGTAEAEFLNVMKTDAMVVGNHEFDDGEDGLAAFLEKVEFPVLGSNVKAQQASKLGDRVK
EYVILDVGGEKIGIVGAVANDTAELSSPGENVSIITDVDGITAAVKAVQDEGVNKIIALTHVGYPRDLEAIAKIPGVDVV
VGGHSHTLLSNTAEGAEGPYPTMVENPEGYQVPVVQAASYSKYLGDLKVVFDDSGVVKEASGEPKLLDAQVQPDEAVLAR
VKELAGPIEELKAQVVSETSASIDGSRETCRARECQMGNLVTDAMLARTKDQGVQFAITNGGGLRASIDAGEVTMGEVLE
VLPFQNTLATFQLTGSDVIASLESGVSQIEEGAGRFPQVSGLRYSFDKSAPAGQGRISNVEVQTADGGWAPIDPNATYTI
ATNNFMRNGGDGYSLFGEKGQNAYDYGPGLEQVLADYLAANNPYKPYTDGRITEVAAAAPSDSAEPPSAEAPATQPSGEA
STTTQPVQPAPAPGETAEATPPAGEAEPAESGTETAAAAPDAGAAEAREAVTTHVIVSGDTLWNLAEEFYGDPMQWRKLA
DANPQLRPNRLVVGATLNIPPAE
>Mature_663_residues
MKNLAVLALGASALALSSAPSFADYTLNILHINDWHSRIQSINAFESTCSDEDESAGKCFGGAGRLVTAVEDARNALQGQ
NVLFLNAGDNFQGSLFYITYKGTAEAEFLNVMKTDAMVVGNHEFDDGEDGLAAFLEKVEFPVLGSNVKAQQASKLGDRVK
EYVILDVGGEKIGIVGAVANDTAELSSPGENVSIITDVDGITAAVKAVQDEGVNKIIALTHVGYPRDLEAIAKIPGVDVV
VGGHSHTLLSNTAEGAEGPYPTMVENPEGYQVPVVQAASYSKYLGDLKVVFDDSGVVKEASGEPKLLDAQVQPDEAVLAR
VKELAGPIEELKAQVVSETSASIDGSRETCRARECQMGNLVTDAMLARTKDQGVQFAITNGGGLRASIDAGEVTMGEVLE
VLPFQNTLATFQLTGSDVIASLESGVSQIEEGAGRFPQVSGLRYSFDKSAPAGQGRISNVEVQTADGGWAPIDPNATYTI
ATNNFMRNGGDGYSLFGEKGQNAYDYGPGLEQVLADYLAANNPYKPYTDGRITEVAAAAPSDSAEPPSAEAPATQPSGEA
STTTQPVQPAPAPGETAEATPPAGEAEPAESGTETAAAAPDAGAAEAREAVTTHVIVSGDTLWNLAEEFYGDPMQWRKLA
DANPQLRPNRLVVGATLNIPPAE

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=547, Percent_Identity=36.1974405850091, Blast_Score=306, Evalue=4e-83,
Organism=Escherichia coli, GI1786687, Length=551, Percent_Identity=26.8602540834846, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1790658, Length=573, Percent_Identity=23.5602094240838, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28573524, Length=497, Percent_Identity=36.8209255533199, Blast_Score=321, Evalue=9e-88,
Organism=Drosophila melanogaster, GI19922446, Length=557, Percent_Identity=35.5475763016158, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24654424, Length=557, Percent_Identity=35.5475763016158, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI19922444, Length=499, Percent_Identity=35.4709418837675, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24641187, Length=528, Percent_Identity=32.0075757575758, Blast_Score=271, Evalue=9e-73,
Organism=Drosophila melanogaster, GI221329836, Length=531, Percent_Identity=31.4500941619586, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24652512, Length=487, Percent_Identity=28.7474332648871, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI161076508, Length=487, Percent_Identity=28.7474332648871, Blast_Score=168, Evalue=9e-42,
Organism=Drosophila melanogaster, GI19921980, Length=487, Percent_Identity=28.7474332648871, Blast_Score=168, Evalue=9e-42,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 69342; Mature: 69342

Theoretical pI: Translated: 4.08; Mature: 4.08

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLAVLALGASALALSSAPSFADYTLNILHINDWHSRIQSINAFESTCSDEDESAGKCF
CCCEEEEEECCHHHHHCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCEE
GGAGRLVTAVEDARNALQGQNVLFLNAGDNFQGSLFYITYKGTAEAEFLNVMKTDAMVVG
CCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCEEEEC
NHEFDDGEDGLAAFLEKVEFPVLGSNVKAQQASKLGDRVKEYVILDVGGEKIGIVGAVAN
CCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCEEEEEEEECC
DTAELSSPGENVSIITDVDGITAAVKAVQDEGVNKIIALTHVGYPRDLEAIAKIPGVDVV
CCHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCEEE
VGGHSHTLLSNTAEGAEGPYPTMVENPEGYQVPVVQAASYSKYLGDLKVVFDDSGVVKEA
ECCCCCEEECCCCCCCCCCCCCCEECCCCCEEEEEECCHHHHHCCCEEEEECCCCCEECC
SGEPKLLDAQVQPDEAVLARVKELAGPIEELKAQVVSETSASIDGSRETCRARECQMGNL
CCCCCEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCH
VTDAMLARTKDQGVQFAITNGGGLRASIDAGEVTMGEVLEVLPFQNTLATFQLTGSDVIA
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHH
SLESGVSQIEEGAGRFPQVSGLRYSFDKSAPAGQGRISNVEVQTADGGWAPIDPNATYTI
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEE
ATNNFMRNGGDGYSLFGEKGQNAYDYGPGLEQVLADYLAANNPYKPYTDGRITEVAAAAP
EECCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
SDSAEPPSAEAPATQPSGEASTTTQPVQPAPAPGETAEATPPAGEAEPAESGTETAAAAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DAGAAEAREAVTTHVIVSGDTLWNLAEEFYGDPMQWRKLADANPQLRPNRLVVGATLNIP
CCCHHHHHHHEEEEEEEECCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEEEEEECCC
PAE
CCC
>Mature Secondary Structure
MKNLAVLALGASALALSSAPSFADYTLNILHINDWHSRIQSINAFESTCSDEDESAGKCF
CCCEEEEEECCHHHHHCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCEE
GGAGRLVTAVEDARNALQGQNVLFLNAGDNFQGSLFYITYKGTAEAEFLNVMKTDAMVVG
CCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCEEEEC
NHEFDDGEDGLAAFLEKVEFPVLGSNVKAQQASKLGDRVKEYVILDVGGEKIGIVGAVAN
CCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCEEEEEEEECC
DTAELSSPGENVSIITDVDGITAAVKAVQDEGVNKIIALTHVGYPRDLEAIAKIPGVDVV
CCHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCEEE
VGGHSHTLLSNTAEGAEGPYPTMVENPEGYQVPVVQAASYSKYLGDLKVVFDDSGVVKEA
ECCCCCEEECCCCCCCCCCCCCCEECCCCCEEEEEECCHHHHHCCCEEEEECCCCCEECC
SGEPKLLDAQVQPDEAVLARVKELAGPIEELKAQVVSETSASIDGSRETCRARECQMGNL
CCCCCEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCH
VTDAMLARTKDQGVQFAITNGGGLRASIDAGEVTMGEVLEVLPFQNTLATFQLTGSDVIA
HHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCHHHHH
SLESGVSQIEEGAGRFPQVSGLRYSFDKSAPAGQGRISNVEVQTADGGWAPIDPNATYTI
HHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEE
ATNNFMRNGGDGYSLFGEKGQNAYDYGPGLEQVLADYLAANNPYKPYTDGRITEVAAAAP
EECCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
SDSAEPPSAEAPATQPSGEASTTTQPVQPAPAPGETAEATPPAGEAEPAESGTETAAAAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DAGAAEAREAVTTHVIVSGDTLWNLAEEFYGDPMQWRKLADANPQLRPNRLVVGATLNIP
CCCHHHHHHHEEEEEEEECCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEEEEEEECCC
PAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]