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The map label for this gene is fixK [H]

Identifier: 110634916

GI number: 110634916

Start: 2763687

End: 2764409

Strand: Reverse

Name: fixK [H]

Synonym: Meso_2575

Alternate gene names: 110634916

Gene position: 2764409-2763687 (Counterclockwise)

Preceding gene: 110634917

Following gene: 110634915

Centisome position: 62.65

GC content: 58.23

Gene sequence:

>723_bases
ATGGCGGAACATTTCGGGGAATTTTTGAAGGCGCTTGACGCGGGCGTTTCATCGCCGGCGGGGAGGGGCCGCAAGGAAGA
GGGCGGTCCTACAGTCTGGCGCCGCAATTATGCGGTAGGAGAAACGATTGCCGAAGATGAGGAGAATAAGCAAGCCTTCC
TTCTGATCACATCCGGCTGGGTCGCGTCAGGCAAGATGCTGACGAACGGCTCGCGAATCGTTCTTGATTTCCTCCTTCCG
GGCGATGTTGTCGGGATCGGCGCCACCGAATGGGCGAGAGAGGTGATCACGGCTCTCTCACCAGTCAGCACTATGGAGGT
CCCCGGCGCGCCGCTGGAAACGTTGCTAAAGGCGCCGCAGACCGTCTCGAGGGCCCTTATAAGAAACGTTGCCCGGCACA
ACGCCAGAATCGCGGAACATATGGTCAGCATCGGACGACGCGGTGCTGTTGAGCGTACCGCGCATCTGCTCCTCGAACTG
GCCTGCCGCCTTGAGATCAAGCCCGATAACGGCAGGGTTCGATTCCAATGCCCGCTCACCCAGGCGGATCTGGCGGACGC
TCTGGGCCTCACCGCAGTCCACGTGAACCGGGTGCTTAAGGAGCTGAGGGAAAATGGGTTGGCTTCGTTTCGAAATGGAA
TCGTTGAGATCCGGGATGTCGACCGGCTTACCGAACTTGTGGATTTCGATGCGTCTTATTTGACTCTTCGAACAGTAAAA
TAG

Upstream 100 bases:

>100_bases
CTTTAGTTTGTTTAAAGAGTATTTGTTGCACATATTGAGTATAAAACAATTTAGCGTGTCATGCATATCCTTAGGTGTGG
ATGAAGGGTATACTTATCAT

Downstream 100 bases:

>100_bases
GACTTTATTCTTCGTAAACTTTAAGTTGAATCAATTTTTTATCAAATGGTGAATAAATTGATAAGGGCTTAATCTAGGTT
AGTGCGCGTTATCCACAATT

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MAEHFGEFLKALDAGVSSPAGRGRKEEGGPTVWRRNYAVGETIAEDEENKQAFLLITSGWVASGKMLTNGSRIVLDFLLP
GDVVGIGATEWAREVITALSPVSTMEVPGAPLETLLKAPQTVSRALIRNVARHNARIAEHMVSIGRRGAVERTAHLLLEL
ACRLEIKPDNGRVRFQCPLTQADLADALGLTAVHVNRVLKELRENGLASFRNGIVEIRDVDRLTELVDFDASYLTLRTVK

Sequences:

>Translated_240_residues
MAEHFGEFLKALDAGVSSPAGRGRKEEGGPTVWRRNYAVGETIAEDEENKQAFLLITSGWVASGKMLTNGSRIVLDFLLP
GDVVGIGATEWAREVITALSPVSTMEVPGAPLETLLKAPQTVSRALIRNVARHNARIAEHMVSIGRRGAVERTAHLLLEL
ACRLEIKPDNGRVRFQCPLTQADLADALGLTAVHVNRVLKELRENGLASFRNGIVEIRDVDRLTELVDFDASYLTLRTVK
>Mature_239_residues
AEHFGEFLKALDAGVSSPAGRGRKEEGGPTVWRRNYAVGETIAEDEENKQAFLLITSGWVASGKMLTNGSRIVLDFLLPG
DVVGIGATEWAREVITALSPVSTMEVPGAPLETLLKAPQTVSRALIRNVARHNARIAEHMVSIGRRGAVERTAHLLLELA
CRLEIKPDNGRVRFQCPLTQADLADALGLTAVHVNRVLKELRENGLASFRNGIVEIRDVDRLTELVDFDASYLTLRTVK

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26244; Mature: 26113

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHFGEFLKALDAGVSSPAGRGRKEEGGPTVWRRNYAVGETIAEDEENKQAFLLITSGW
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCCEEEEEEECCC
VASGKMLTNGSRIVLDFLLPGDVVGIGATEWAREVITALSPVSTMEVPGAPLETLLKAPQ
EECCEEEECCCEEEEEEECCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHH
TVSRALIRNVARHNARIAEHMVSIGRRGAVERTAHLLLELACRLEIKPDNGRVRFQCPLT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCCEEEEECCCC
QADLADALGLTAVHVNRVLKELRENGLASFRNGIVEIRDVDRLTELVDFDASYLTLRTVK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
AEHFGEFLKALDAGVSSPAGRGRKEEGGPTVWRRNYAVGETIAEDEENKQAFLLITSGW
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCCEEEEEEECCC
VASGKMLTNGSRIVLDFLLPGDVVGIGATEWAREVITALSPVSTMEVPGAPLETLLKAPQ
EECCEEEECCCEEEEEEECCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHH
TVSRALIRNVARHNARIAEHMVSIGRRGAVERTAHLLLELACRLEIKPDNGRVRFQCPLT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECCCCEEEEECCCC
QADLADALGLTAVHVNRVLKELRENGLASFRNGIVEIRDVDRLTELVDFDASYLTLRTVK
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEHHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]