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The map label for this gene is fixJ [H]

Identifier: 110634571

GI number: 110634571

Start: 2370745

End: 2371362

Strand: Reverse

Name: fixJ [H]

Synonym: Meso_2222

Alternate gene names: 110634571

Gene position: 2371362-2370745 (Counterclockwise)

Preceding gene: 110634572

Following gene: 110634570

Centisome position: 53.74

GC content: 62.46

Gene sequence:

>618_bases
ATGAGCGGTGATGTCGTAATCCATATCGTTGACGACGAGGAGCCTGTCAGGAAATCGCTGGCATTCCTCCTCACCATATC
GGGGCACGCGGTGCGCGTGCACGACTCGGCAAGCTCCTTCCTCTCCGTTGCCGGGGAACTGGAGAACGCCTGCCTTGTCA
CCGATCTGCGCATGCCGGATATGAGCGGCGTCGACCTGCTCAGGCGGCTGCACGATGAGGATGCGATGCTGCCCGCCATC
GTGATCACGGGGCATGGGGACGTTCCGATGGCAGTAGAGGCGATGAAGGCGGGCGCGCTCGATTTCATCGAGAAGCCATT
CGAGGACGAGGTCCTGCTCGAAGCAGTGGCACGCGCCATCGCCGAGCTCAAAAGGAAGCCGCGGGAGCAGGACGACATTT
CGGCCATACGCCAGCGGCTCGGCCAGCTCAGCGAACGGGAGAGACAGGTCATGGCCGGAATCGTCGCGGGGCTACCCAAC
AAAACCATCGCCTACGACCTCAATATCAGCCCGCGAACGGTCGAGGTCCACCGCTCGAATGTCATGGCAAAGATGACCGC
CAAGAACCTGCCCGAGCTCGTCCGGATGGTCTTGGCGGCGGGCGACGAGAGCTCCTGA

Upstream 100 bases:

>100_bases
ATCGAGGCTCACGGGGGCGAACTGATCATGCAGCGGAACGAGAAGGGTGGCGCGACCTTCCGCTTCACCCTGCCGGCCGG
AAAAGAGCAGGAGCCGGAAA

Downstream 100 bases:

>100_bases
GACGCGCTTGGTGGCTTGATCCAGCGCAAGGCGGTCTGGTCTCCTGTCAGTGCAGTGTAGCCGATGGCCTGAGAGGCCGA
TTTCTGAAAAAGCAGATCAA

Product: response regulator FixJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MSGDVVIHIVDDEEPVRKSLAFLLTISGHAVRVHDSASSFLSVAGELENACLVTDLRMPDMSGVDLLRRLHDEDAMLPAI
VITGHGDVPMAVEAMKAGALDFIEKPFEDEVLLEAVARAIAELKRKPREQDDISAIRQRLGQLSERERQVMAGIVAGLPN
KTIAYDLNISPRTVEVHRSNVMAKMTAKNLPELVRMVLAAGDESS

Sequences:

>Translated_205_residues
MSGDVVIHIVDDEEPVRKSLAFLLTISGHAVRVHDSASSFLSVAGELENACLVTDLRMPDMSGVDLLRRLHDEDAMLPAI
VITGHGDVPMAVEAMKAGALDFIEKPFEDEVLLEAVARAIAELKRKPREQDDISAIRQRLGQLSERERQVMAGIVAGLPN
KTIAYDLNISPRTVEVHRSNVMAKMTAKNLPELVRMVLAAGDESS
>Mature_204_residues
SGDVVIHIVDDEEPVRKSLAFLLTISGHAVRVHDSASSFLSVAGELENACLVTDLRMPDMSGVDLLRRLHDEDAMLPAIV
ITGHGDVPMAVEAMKAGALDFIEKPFEDEVLLEAVARAIAELKRKPREQDDISAIRQRLGQLSERERQVMAGIVAGLPNK
TIAYDLNISPRTVEVHRSNVMAKMTAKNLPELVRMVLAAGDESS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=33.1632653061224, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790299, Length=125, Percent_Identity=31.2, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=31.5789473684211, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788521, Length=193, Percent_Identity=31.6062176165803, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788905, Length=114, Percent_Identity=30.7017543859649, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22387; Mature: 22256

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDVVIHIVDDEEPVRKSLAFLLTISGHAVRVHDSASSFLSVAGELENACLVTDLRMPD
CCCCEEEEEECCCHHHHHHHHHHEEECCCEEEEECCHHHHHHHHHHHCCCEEEEECCCCC
MSGVDLLRRLHDEDAMLPAIVITGHGDVPMAVEAMKAGALDFIEKPFEDEVLLEAVARAI
CCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHH
AELKRKPREQDDISAIRQRLGQLSERERQVMAGIVAGLPNKTIAYDLNISPRTVEVHRSN
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEHHHH
VMAKMTAKNLPELVRMVLAAGDESS
HHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGDVVIHIVDDEEPVRKSLAFLLTISGHAVRVHDSASSFLSVAGELENACLVTDLRMPD
CCCEEEEEECCCHHHHHHHHHHEEECCCEEEEECCHHHHHHHHHHHCCCEEEEECCCCC
MSGVDLLRRLHDEDAMLPAIVITGHGDVPMAVEAMKAGALDFIEKPFEDEVLLEAVARAI
CCHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHH
AELKRKPREQDDISAIRQRLGQLSERERQVMAGIVAGLPNKTIAYDLNISPRTVEVHRSN
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEHHHH
VMAKMTAKNLPELVRMVLAAGDESS
HHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]