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The map label for this gene is fixL [H]

Identifier: 110634572

GI number: 110634572

Start: 2371352

End: 2372725

Strand: Reverse

Name: fixL [H]

Synonym: Meso_2223

Alternate gene names: 110634572

Gene position: 2372725-2371352 (Counterclockwise)

Preceding gene: 110634574

Following gene: 110634571

Centisome position: 53.77

GC content: 62.95

Gene sequence:

>1374_bases
ATGGATCAGACGTGGTTGTCGCTGCTGGTGCCGATGGCCGTTCTGGGCGCAGCCATCTGGGGAGGGAGGCGCGCGGGCTT
CCTGGTCTTGGCCGTCGGCGCCGCCTTTCTCGCGGCAGGCGCAGCTGGGGTGATGGCGAACTTCAGCGCCGTGCCGGCGC
TTTTGTCGTTTTTGTTCTCCGCCGCGTTGGTCTTCGTCGGCTCCGGCTACCGTGACGCCAGGAAAATCGCTCGTGGCGCG
CAGGAACAGCTCGATGCGCGTGAAGCCCATCTTCGCTCCATTCTGGATACGGTGCCGGACGCGACGGTGGTCATTGATGT
GCAGGGCACGATGATCTCTTTCAACCGCGCTGCCGTTCGCCAGTTCGGCTATTCGGAAGCCGAAGCGGTAGGGCAGAACG
TCAAGATCCTGATGCCGGAGCCTTACCAGGGCGAACATGACGGCTACATCCACCGCTATCTGACAACCGGCGAGCGGCGG
ATCATCGGGATCGACCGTGTCGTCGTCGGGCGGCGGAAGGACGGCTCGACCTTCCCCATGAAGCTTGCGGTGGGTGAGAT
GAAGAGCGGTGGCCAGGTGTTCTTCACTGGCTTCATCCGCGACCTGACGGAAAGGGCCGAGTCGGAGGCGAGACTGGAGG
AGATCCAGGCAGAGCTCGCGCGCCTGGCGCGTCTGAACGAACTGGGAGAAATGGCCTCGACCCTCGCACACGAGCTCAAC
CAGCCGCTTTCGGCCATCGCTAACTATGTGCAGGGGTGTAGCCGGCTTCTAAAGAACATGGATGATGCGCTGGCCTCACG
TATGCGCGAGGCGCTGGATGAGACCGCTCGCCAGACGCTGCGCGCCGGGCAGATCATTCGCCACCTACGCGAGTTCGTCA
CCAGGGGCGAGACCGAAAAGCAGCCGGAAGACCTCCATAAGCTCGTCGAGGAGGCCGGCGCTCTCGCCCTCGTCGGCTCG
CGCGAATTGGGTGTGCGGACGGTGTTCGACTTCAAGGCCGGCCCGAAGGTGGTTCTGGTTGACCGTGTCCAGATACAGCA
GGTGCTGATCAACCTTATGCGCAACGCCATGGAAGCCATGAAGGAGAGCGAAGCCCGCGAGTTGGTGGTACGAATCATGC
AGGGCGAAGGCGATGAAGCGGTGGTGGAAGTTTCCGACACGGGCTCAGGCGTGCCGGACGACGTGGTGGCACAGCTATTC
CAGCCCTTCGTCACCACTAAGCCCGGCGGGATGGGAATAGGGCTCTCCATATCGAAGCGGATCATCGAGGCTCACGGGGG
CGAACTGATCATGCAGCGGAACGAGAAGGGTGGCGCGACCTTCCGCTTCACCCTGCCGGCCGGAAAAGAGCAGGAGCCGG
AAAATGAGCGGTGA

Upstream 100 bases:

>100_bases
TGCCGCATTGCGGAATGGTGGCGATTTTCCCGACGCCGCCCTATGGTAATGTCCGGTAATCGAAACGCTTTCATTGGGGC
GGCAAGTGCTCTTCCGGGGA

Downstream 100 bases:

>100_bases
TGTCGTAATCCATATCGTTGACGACGAGGAGCCTGTCAGGAAATCGCTGGCATTCCTCCTCACCATATCGGGGCACGCGG
TGCGCGTGCACGACTCGGCA

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDQTWLSLLVPMAVLGAAIWGGRRAGFLVLAVGAAFLAAGAAGVMANFSAVPALLSFLFSAALVFVGSGYRDARKIARGA
QEQLDAREAHLRSILDTVPDATVVIDVQGTMISFNRAAVRQFGYSEAEAVGQNVKILMPEPYQGEHDGYIHRYLTTGERR
IIGIDRVVVGRRKDGSTFPMKLAVGEMKSGGQVFFTGFIRDLTERAESEARLEEIQAELARLARLNELGEMASTLAHELN
QPLSAIANYVQGCSRLLKNMDDALASRMREALDETARQTLRAGQIIRHLREFVTRGETEKQPEDLHKLVEEAGALALVGS
RELGVRTVFDFKAGPKVVLVDRVQIQQVLINLMRNAMEAMKESEARELVVRIMQGEGDEAVVEVSDTGSGVPDDVVAQLF
QPFVTTKPGGMGIGLSISKRIIEAHGGELIMQRNEKGGATFRFTLPAGKEQEPENER

Sequences:

>Translated_457_residues
MDQTWLSLLVPMAVLGAAIWGGRRAGFLVLAVGAAFLAAGAAGVMANFSAVPALLSFLFSAALVFVGSGYRDARKIARGA
QEQLDAREAHLRSILDTVPDATVVIDVQGTMISFNRAAVRQFGYSEAEAVGQNVKILMPEPYQGEHDGYIHRYLTTGERR
IIGIDRVVVGRRKDGSTFPMKLAVGEMKSGGQVFFTGFIRDLTERAESEARLEEIQAELARLARLNELGEMASTLAHELN
QPLSAIANYVQGCSRLLKNMDDALASRMREALDETARQTLRAGQIIRHLREFVTRGETEKQPEDLHKLVEEAGALALVGS
RELGVRTVFDFKAGPKVVLVDRVQIQQVLINLMRNAMEAMKESEARELVVRIMQGEGDEAVVEVSDTGSGVPDDVVAQLF
QPFVTTKPGGMGIGLSISKRIIEAHGGELIMQRNEKGGATFRFTLPAGKEQEPENER
>Mature_457_residues
MDQTWLSLLVPMAVLGAAIWGGRRAGFLVLAVGAAFLAAGAAGVMANFSAVPALLSFLFSAALVFVGSGYRDARKIARGA
QEQLDAREAHLRSILDTVPDATVVIDVQGTMISFNRAAVRQFGYSEAEAVGQNVKILMPEPYQGEHDGYIHRYLTTGERR
IIGIDRVVVGRRKDGSTFPMKLAVGEMKSGGQVFFTGFIRDLTERAESEARLEEIQAELARLARLNELGEMASTLAHELN
QPLSAIANYVQGCSRLLKNMDDALASRMREALDETARQTLRAGQIIRHLREFVTRGETEKQPEDLHKLVEEAGALALVGS
RELGVRTVFDFKAGPKVVLVDRVQIQQVLINLMRNAMEAMKESEARELVVRIMQGEGDEAVVEVSDTGSGVPDDVVAQLF
QPFVTTKPGGMGIGLSISKRIIEAHGGELIMQRNEKGGATFRFTLPAGKEQEPENER

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=370, Percent_Identity=30.2702702702703, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI48994928, Length=387, Percent_Identity=25.8397932816537, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790436, Length=242, Percent_Identity=30.1652892561983, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788713, Length=414, Percent_Identity=24.6376811594203, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1790300, Length=263, Percent_Identity=28.8973384030418, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081816, Length=114, Percent_Identity=35.9649122807018, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789149, Length=260, Percent_Identity=26.5384615384615, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI145693157, Length=271, Percent_Identity=25.830258302583, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786837, Length=251, Percent_Identity=27.4900398406374, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081921, Length=108, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49875; Mature: 49875

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTWLSLLVPMAVLGAAIWGGRRAGFLVLAVGAAFLAAGAAGVMANFSAVPALLSFLFS
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALVFVGSGYRDARKIARGAQEQLDAREAHLRSILDTVPDATVVIDVQGTMISFNRAAVR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHH
QFGYSEAEAVGQNVKILMPEPYQGEHDGYIHRYLTTGERRIIGIDRVVVGRRKDGSTFPM
HCCCHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEEECCEEEECCCCCCCCCE
KLAVGEMKSGGQVFFTGFIRDLTERAESEARLEEIQAELARLARLNELGEMASTLAHELN
EEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QPLSAIANYVQGCSRLLKNMDDALASRMREALDETARQTLRAGQIIRHLREFVTRGETEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QPEDLHKLVEEAGALALVGSRELGVRTVFDFKAGPKVVLVDRVQIQQVLINLMRNAMEAM
CHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
KESEARELVVRIMQGEGDEAVVEVSDTGSGVPDDVVAQLFQPFVTTKPGGMGIGLSISKR
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEECHHHHHH
IIEAHGGELIMQRNEKGGATFRFTLPAGKEQEPENER
HHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MDQTWLSLLVPMAVLGAAIWGGRRAGFLVLAVGAAFLAAGAAGVMANFSAVPALLSFLFS
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALVFVGSGYRDARKIARGAQEQLDAREAHLRSILDTVPDATVVIDVQGTMISFNRAAVR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHH
QFGYSEAEAVGQNVKILMPEPYQGEHDGYIHRYLTTGERRIIGIDRVVVGRRKDGSTFPM
HCCCHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEEECCEEEECCCCCCCCCE
KLAVGEMKSGGQVFFTGFIRDLTERAESEARLEEIQAELARLARLNELGEMASTLAHELN
EEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QPLSAIANYVQGCSRLLKNMDDALASRMREALDETARQTLRAGQIIRHLREFVTRGETEK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QPEDLHKLVEEAGALALVGSRELGVRTVFDFKAGPKVVLVDRVQIQQVLINLMRNAMEAM
CHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
KESEARELVVRIMQGEGDEAVVEVSDTGSGVPDDVVAQLFQPFVTTKPGGMGIGLSISKR
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEECHHHHHH
IIEAHGGELIMQRNEKGGATFRFTLPAGKEQEPENER
HHHHCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]