Click here to switch to the map view.

The map label for this gene is fixK [H]

Identifier: 110634569

GI number: 110634569

Start: 2369526

End: 2370200

Strand: Reverse

Name: fixK [H]

Synonym: Meso_2220

Alternate gene names: 110634569

Gene position: 2370200-2369526 (Counterclockwise)

Preceding gene: 110634570

Following gene: 110634568

Centisome position: 53.72

GC content: 60.3

Gene sequence:

>675_bases
ATGCTCTCTCACAACGCCATACAAGCTTCCTCAACTAGAATTCATCCAGACGTTCCCGTCACTCGCCACACCGCGACACA
ACTGGTACCACCTGCCTTTGCGGCCTCGCAGCCGCATCAGGTGATCCTCTATGCGTCCAACGCGGAAATCTATGGTCAGA
GCGAGCGAGCCACCGCTCTCTACCAGGTGGAGTTCGGCGCGGTTCGCGTCTACCGCCTACTTTCCGATGGTCGTCGGCAG
ATCAGTGCCTTCCACGTGGCAGGCGAGGTCTTCGGCTTCGAGGCAGACGGCCACCATCATTTCTTCGCCGAGGCGATCTG
CAATTCCGCGGTCCGCATCTTTCGCCAACCGCTCGGTGCCGACGTCGCGCGCGGCATCCTTCCGATCGCGCTGAAGGCAT
TGATCCGCGCGCAGGAGCACCTGCTCGTCATCGGGCGTCAGAATGCCGCCGAGCGCGTCGCAGCCTTCCTGCTTGATCAA
CTCGAACGTCAGGGCGGCCTTCCGCAGGTCGAACTCGCCATGTCGCGCACCGATATCGGCGACTATCTGGGCTTGACAAT
CGAGACGGTCTCGCGCGTTTTCTCGAAGTTCCGTGAAAAGGGCTATATCCGGCTCCAAGGCGTGCGTACTGTGGAACTTA
TTAAGCCAGAGGCGCTGCGTGCTCTTTGTGTCTGA

Upstream 100 bases:

>100_bases
GCGGCCCGCCATGCCTACGTAGTTTCCCTTAGGATATAACCGAATTTCTTCGATCGGTGTTCCGGGGCACTGATGGTCCG
ACAAGATCGGAGATATCGAG

Downstream 100 bases:

>100_bases
GTTCCTGCCGAGCGCGGAGTCGAGATTGGTTCTGCAGGAAGATATGCAAGCAAAAAACGCCGGGTACCGCGTGGTTGACG
CAGCGATGGGTGCTTCGACG

Product: transcriptional regulator FixK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MLSHNAIQASSTRIHPDVPVTRHTATQLVPPAFAASQPHQVILYASNAEIYGQSERATALYQVEFGAVRVYRLLSDGRRQ
ISAFHVAGEVFGFEADGHHHFFAEAICNSAVRIFRQPLGADVARGILPIALKALIRAQEHLLVIGRQNAAERVAAFLLDQ
LERQGGLPQVELAMSRTDIGDYLGLTIETVSRVFSKFREKGYIRLQGVRTVELIKPEALRALCV

Sequences:

>Translated_224_residues
MLSHNAIQASSTRIHPDVPVTRHTATQLVPPAFAASQPHQVILYASNAEIYGQSERATALYQVEFGAVRVYRLLSDGRRQ
ISAFHVAGEVFGFEADGHHHFFAEAICNSAVRIFRQPLGADVARGILPIALKALIRAQEHLLVIGRQNAAERVAAFLLDQ
LERQGGLPQVELAMSRTDIGDYLGLTIETVSRVFSKFREKGYIRLQGVRTVELIKPEALRALCV
>Mature_224_residues
MLSHNAIQASSTRIHPDVPVTRHTATQLVPPAFAASQPHQVILYASNAEIYGQSERATALYQVEFGAVRVYRLLSDGRRQ
ISAFHVAGEVFGFEADGHHHFFAEAICNSAVRIFRQPLGADVARGILPIALKALIRAQEHLLVIGRQNAAERVAAFLLDQ
LERQGGLPQVELAMSRTDIGDYLGLTIETVSRVFSKFREKGYIRLQGVRTVELIKPEALRALCV

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=173, Percent_Identity=32.9479768786127, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24749; Mature: 24749

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHNAIQASSTRIHPDVPVTRHTATQLVPPAFAASQPHQVILYASNAEIYGQSERATAL
CCCCCCCCCCCCEECCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCEEECCCCCCEEE
YQVEFGAVRVYRLLSDGRRQISAFHVAGEVFGFEADGHHHFFAEAICNSAVRIFRQPLGA
EEEEHHHHHHHHHHHHHHHHHHHHEEHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCH
DVARGILPIALKALIRAQEHLLVIGRQNAAERVAAFLLDQLERQGGLPQVELAMSRTDIG
HHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCH
DYLGLTIETVSRVFSKFREKGYIRLQGVRTVELIKPEALRALCV
HHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECHHHHHHHCC
>Mature Secondary Structure
MLSHNAIQASSTRIHPDVPVTRHTATQLVPPAFAASQPHQVILYASNAEIYGQSERATAL
CCCCCCCCCCCCEECCCCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCEEECCCCCCEEE
YQVEFGAVRVYRLLSDGRRQISAFHVAGEVFGFEADGHHHFFAEAICNSAVRIFRQPLGA
EEEEHHHHHHHHHHHHHHHHHHHHEEHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCH
DVARGILPIALKALIRAQEHLLVIGRQNAAERVAAFLLDQLERQGGLPQVELAMSRTDIG
HHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCH
DYLGLTIETVSRVFSKFREKGYIRLQGVRTVELIKPEALRALCV
HHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663474; 11481432 [H]