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The map label for this gene is cca [H]

Identifier: 110634525

GI number: 110634525

Start: 2324379

End: 2325608

Strand: Reverse

Name: cca [H]

Synonym: Meso_2176

Alternate gene names: 110634525

Gene position: 2325608-2324379 (Counterclockwise)

Preceding gene: 110634526

Following gene: 110634520

Centisome position: 52.71

GC content: 64.23

Gene sequence:

>1230_bases
ATGACCACGATCGCCGGTGCTCCCTGGCTCGATCAGCAGGGCCTTCAGCCACTGCTGGAAATCCTCAACGAAGGCGGGGA
AGAAGCCCGCATCGCAGGCGGCGCTGTCCGCAATGCGCTTCTTGGCGAGGCGGTGACCGATATCGATATCGCCACCACCA
ATCTTCCCGACGAGACGATCCGCCGGGCGAAAGCAGCGGGCTTCAAGGTCGTGCCGACCGGCTATGAACACGGCACGATC
ACCGTAATCGCCAAGGGCACCCCTTACGAGGTTACAACCCTGCGCGCGGATGTGGAAACGTTCGGGCGGCACGCGCGCGT
GGTCTTCGGGCGTGATTGGAAAGCGGATGCGGAGCGGCGGGACTTCACGATCAATGCGCTTTACGCGGAGCCCGGTGGCC
GGGTGGTCGACCTTGTCGGCGGCCTGGCGGATCTGCAATCGCGCACTCTCCGCTTCATCGGCGAGCCGGAAAAACGCATC
CGTGAAGATTATCTGCGTATCCTCCGGTTCTTCCGCTTCTTTGCCTGGTACGGCTCGGGCCGGCCGGATGCGGAGGGCCT
AAAGGCCTGCGCGCGGCTGAAGGACCGGCTTTCGGGCCTCTCCGTCGAGCGCATCTGGATGGAGTTGAAGAAACTTCTGG
CCGCTCCAGATCCCTCGCGCGCGTTGCTTTGGATGCGGCAGACGGGCGTGCTTACGCAGGTTCTGCCCGAAACGGAAAAA
TGGGGCATCGACGCGATTCACTCGCTGGTCGAGACGGAGCGGGCGCTCGGCTGGCCGGTGGAGCCTCTCCTGCGGCTGGC
CTCGATCGTGCCCCCGGACCCGGAGCGTATCGAAAAGCTCGCAGCGCGACTGCGCCTTTCCAAGGCGGAGGGTGCCGCCC
TGGTATCCTGGGCAGAGTCTTCCCAGCCGGACCCGTCGCTGAAGGAGGGAGAGTTCCTGAAGCTCCTTTACCGAAGCGAG
GCTGGCGGCATGGCATGGCGGCTGCGGCTCGGCCTTGCTTCCGCCCGGGCGCGCGCTGTCGAGAGCGACAAGGCGCTGAT
CGAGGCCGCCGGCTACCAACGCCTGCTCGATATTACCGCGCGCTGGGAAAAGCCGGTGTTCCCTGTTCGGGGCAGGGATC
TGCTGGAAAGAGGCTTCAAGCCGGGGAAGGAACTTGGCGCAAAGCTGGCCGAACTCGAAGAGAAATGGATTGCCTCGGGC
TTTTCGCTTTCGCGCGAAGATCTGCTTTAA

Upstream 100 bases:

>100_bases
AGAGGGAACGCTTTTTCTACACAATGCCTTACGGCGAGCGCTTCATATGGGGAATCACCGCCGGCATTCTGCATATGCTT
TATGAAAGGCTCTATGCCCG

Downstream 100 bases:

>100_bases
AAAACAGAAGGGCGCCTTAGGCGCCCCTTCCTTTGATCTGTTGAACGGCTGTCTACGCAGCGTCCTGCTGCTGGACGATC
CGGGAGCGGATCGCCTCGAT

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTTIAGAPWLDQQGLQPLLEILNEGGEEARIAGGAVRNALLGEAVTDIDIATTNLPDETIRRAKAAGFKVVPTGYEHGTI
TVIAKGTPYEVTTLRADVETFGRHARVVFGRDWKADAERRDFTINALYAEPGGRVVDLVGGLADLQSRTLRFIGEPEKRI
REDYLRILRFFRFFAWYGSGRPDAEGLKACARLKDRLSGLSVERIWMELKKLLAAPDPSRALLWMRQTGVLTQVLPETEK
WGIDAIHSLVETERALGWPVEPLLRLASIVPPDPERIEKLAARLRLSKAEGAALVSWAESSQPDPSLKEGEFLKLLYRSE
AGGMAWRLRLGLASARARAVESDKALIEAAGYQRLLDITARWEKPVFPVRGRDLLERGFKPGKELGAKLAELEEKWIASG
FSLSREDLL

Sequences:

>Translated_409_residues
MTTIAGAPWLDQQGLQPLLEILNEGGEEARIAGGAVRNALLGEAVTDIDIATTNLPDETIRRAKAAGFKVVPTGYEHGTI
TVIAKGTPYEVTTLRADVETFGRHARVVFGRDWKADAERRDFTINALYAEPGGRVVDLVGGLADLQSRTLRFIGEPEKRI
REDYLRILRFFRFFAWYGSGRPDAEGLKACARLKDRLSGLSVERIWMELKKLLAAPDPSRALLWMRQTGVLTQVLPETEK
WGIDAIHSLVETERALGWPVEPLLRLASIVPPDPERIEKLAARLRLSKAEGAALVSWAESSQPDPSLKEGEFLKLLYRSE
AGGMAWRLRLGLASARARAVESDKALIEAAGYQRLLDITARWEKPVFPVRGRDLLERGFKPGKELGAKLAELEEKWIASG
FSLSREDLL
>Mature_408_residues
TTIAGAPWLDQQGLQPLLEILNEGGEEARIAGGAVRNALLGEAVTDIDIATTNLPDETIRRAKAAGFKVVPTGYEHGTIT
VIAKGTPYEVTTLRADVETFGRHARVVFGRDWKADAERRDFTINALYAEPGGRVVDLVGGLADLQSRTLRFIGEPEKRIR
EDYLRILRFFRFFAWYGSGRPDAEGLKACARLKDRLSGLSVERIWMELKKLLAAPDPSRALLWMRQTGVLTQVLPETEKW
GIDAIHSLVETERALGWPVEPLLRLASIVPPDPERIEKLAARLRLSKAEGAALVSWAESSQPDPSLKEGEFLKLLYRSEA
GGMAWRLRLGLASARARAVESDKALIEAAGYQRLLDITARWEKPVFPVRGRDLLERGFKPGKELGAKLAELEEKWIASGF
SLSREDLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=414, Percent_Identity=35.2657004830918, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI87081691, Length=204, Percent_Identity=34.3137254901961, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789436, Length=268, Percent_Identity=29.8507462686567, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=406, Percent_Identity=32.2660098522167, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI281360112, Length=403, Percent_Identity=33.2506203473945, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI21357337, Length=403, Percent_Identity=33.2506203473945, Blast_Score=187, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45427; Mature: 45296

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIAGAPWLDQQGLQPLLEILNEGGEEARIAGGAVRNALLGEAVTDIDIATTNLPDETI
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
RRAKAAGFKVVPTGYEHGTITVIAKGTPYEVTTLRADVETFGRHARVVFGRDWKADAERR
HHHHHCCCEEEECCCCCCEEEEEECCCCCEEEEEHHHHHHHCCCEEEEECCCCCCCCCCC
DFTINALYAEPGGRVVDLVGGLADLQSRTLRFIGEPEKRIREDYLRILRFFRFFAWYGSG
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
RPDAEGLKACARLKDRLSGLSVERIWMELKKLLAAPDPSRALLWMRQTGVLTQVLPETEK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHEEEEHHHCHHHHHCCCCHH
WGIDAIHSLVETERALGWPVEPLLRLASIVPPDPERIEKLAARLRLSKAEGAALVSWAES
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
SQPDPSLKEGEFLKLLYRSEAGGMAWRLRLGLASARARAVESDKALIEAAGYQRLLDITA
CCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWEKPVFPVRGRDLLERGFKPGKELGAKLAELEEKWIASGFSLSREDLL
HCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
TTIAGAPWLDQQGLQPLLEILNEGGEEARIAGGAVRNALLGEAVTDIDIATTNLPDETI
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
RRAKAAGFKVVPTGYEHGTITVIAKGTPYEVTTLRADVETFGRHARVVFGRDWKADAERR
HHHHHCCCEEEECCCCCCEEEEEECCCCCEEEEEHHHHHHHCCCEEEEECCCCCCCCCCC
DFTINALYAEPGGRVVDLVGGLADLQSRTLRFIGEPEKRIREDYLRILRFFRFFAWYGSG
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
RPDAEGLKACARLKDRLSGLSVERIWMELKKLLAAPDPSRALLWMRQTGVLTQVLPETEK
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHEEEEHHHCHHHHHCCCCHH
WGIDAIHSLVETERALGWPVEPLLRLASIVPPDPERIEKLAARLRLSKAEGAALVSWAES
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
SQPDPSLKEGEFLKLLYRSEAGGMAWRLRLGLASARARAVESDKALIEAAGYQRLLDITA
CCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWEKPVFPVRGRDLLERGFKPGKELGAKLAELEEKWIASGFSLSREDLL
HCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA