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The map label for this gene is murE

Identifier: 110634362

GI number: 110634362

Start: 2160073

End: 2161524

Strand: Reverse

Name: murE

Synonym: Meso_2012

Alternate gene names: 110634362

Gene position: 2161524-2160073 (Counterclockwise)

Preceding gene: 110634363

Following gene: 110634361

Centisome position: 48.99

GC content: 64.26

Gene sequence:

>1452_bases
ATGAAGCTGGGCGAATTTGCCGATATCCTGCCGCTGAGCGGATCCATAGCGGGTGAAACGACGATCTCCGGTCTTGCATC
GGATTCCCGGCGGGTGAACGCCGGGGATCTTTTCTTTGCGTTGCAAGGCTCAAAAGCCGACGGCGCCGCCTATGCGGCTG
ATGCCGCGGGCCGGGGCGCCGTGGCCATCATCGCCAGGCCGGGGACGGTTGGCGATGTCGGGGTGCCCGTCATCGAAGCC
GATGATCCCCGACATGTGCTGGCGCTTGCTTCAGCCCGGCTCTATGGCGCTCAGCCTGCCACTATGGTCGCAGTGACCGG
AACCAGCGGGAAAACCTCGGTCGCCGCCTTCACGCGCCAGATCTGGGAGAAGGCGGGCCTTGCCGCCGCCTCGATCGGAA
CCACCGGTGTCGTGGCCCCCGGCCGCGATGAATATGGCGAGCTTACAACGCCCGATCCGGTCTCCCTTCACAAGCTCCTG
CGCGAGCTTGCGGATGCGGGTGTCACCCACGCCTCCATGGAGGCTTCGAGCCACGGGCTCGACCAGCGCCGGCTCGACGG
TGTGAAGCTTGCCGCGGGCGGCTTCACCAATCTGGGCAGAGACCATATGGATTACCATCCAACGGTCGAGCACTATCATC
AGTCTAAGATGCGGCTGTTCAAAACGCTGCTGCCAAAGGGCGCTCCTGCGGTCATCTTCGCCGACGATCCGTGGTCGGAA
CCTACGGAAAAGGTCGCTCTTGCGGCGGGCCTCCAACTTCTCACCGTCGGCCGCAGGGGAACCTTCCTGACGATCAAGCG
CGTAGAGCACGAGCGTCACCGGCAAAGGACGGAGATTGAACACGAGGGCATGATCTATGACATCATGCTGCCGCTCGCCG
GCGATTTCCAGGTCGGCAACGCGCTCGTGGCGGCGGGGCTCGCCATCTCCACGGGTCTTCCCGCCGACAAGGCCGTTGCG
GCGCTGGAGCACCTGAAAGGTGCTTCCGGGCGGCTGGAACTCACCGGCACAACGGAAGACGGCGCGCAGATCTATGTGGA
TTACGCGCACAAGCCCGACGCGCTTGAGAATGTGCTCCAGGCGGTGCGGCCCTTCACGACCGGCCGCGTCATCGTCGTTT
TCGGCTGCGGCGGCGACCGCGATCCCGGAAAGCGGCCAATCATGGGGGAAATCGCGCGTCGGCTTGCGGATGTGGTGATC
GTGACCGACGATAACCCGCGCTCGGAAGTACCCGCGGAGATCCGCAGGGCGATCCTGGAGGCGGTGCCGGAGGCAACCGA
AATCGGCGACCGGCGCGAGGCCATACGGACCGCTGTCGCGATGCTTAACTCAGGCGATACATTAATTGTCGCGGGCAAGG
GCCATGAAATCGGCCAGGAAATCGCGGGCACAAAACTCCCGTTTTCCGATCACGAGGAGGTGCGGCGGGCGCTGAAGGAG
AGGACTGCGTGA

Upstream 100 bases:

>100_bases
TGGAGCCGGATTTCAGCCATGAAAGTGACGCTTTGCTGGTCTCCTACCGGTGATTCCGGACACAACGAGCGGAACGGACC
GCAGAGAGTACTGGGATGCG

Downstream 100 bases:

>100_bases
CGCTTCTATGGACTTCGGACGACCTTGTGGCGGCTGTCGGCGGCCGCCCCTTCGGGGCGCTGCCCGAAGGCGTGACGGGC
ATTTCCATCGACAGCAGAAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKLGEFADILPLSGSIAGETTISGLASDSRRVNAGDLFFALQGSKADGAAYAADAAGRGAVAIIARPGTVGDVGVPVIEA
DDPRHVLALASARLYGAQPATMVAVTGTSGKTSVAAFTRQIWEKAGLAAASIGTTGVVAPGRDEYGELTTPDPVSLHKLL
RELADAGVTHASMEASSHGLDQRRLDGVKLAAGGFTNLGRDHMDYHPTVEHYHQSKMRLFKTLLPKGAPAVIFADDPWSE
PTEKVALAAGLQLLTVGRRGTFLTIKRVEHERHRQRTEIEHEGMIYDIMLPLAGDFQVGNALVAAGLAISTGLPADKAVA
ALEHLKGASGRLELTGTTEDGAQIYVDYAHKPDALENVLQAVRPFTTGRVIVVFGCGGDRDPGKRPIMGEIARRLADVVI
VTDDNPRSEVPAEIRRAILEAVPEATEIGDRREAIRTAVAMLNSGDTLIVAGKGHEIGQEIAGTKLPFSDHEEVRRALKE
RTA

Sequences:

>Translated_483_residues
MKLGEFADILPLSGSIAGETTISGLASDSRRVNAGDLFFALQGSKADGAAYAADAAGRGAVAIIARPGTVGDVGVPVIEA
DDPRHVLALASARLYGAQPATMVAVTGTSGKTSVAAFTRQIWEKAGLAAASIGTTGVVAPGRDEYGELTTPDPVSLHKLL
RELADAGVTHASMEASSHGLDQRRLDGVKLAAGGFTNLGRDHMDYHPTVEHYHQSKMRLFKTLLPKGAPAVIFADDPWSE
PTEKVALAAGLQLLTVGRRGTFLTIKRVEHERHRQRTEIEHEGMIYDIMLPLAGDFQVGNALVAAGLAISTGLPADKAVA
ALEHLKGASGRLELTGTTEDGAQIYVDYAHKPDALENVLQAVRPFTTGRVIVVFGCGGDRDPGKRPIMGEIARRLADVVI
VTDDNPRSEVPAEIRRAILEAVPEATEIGDRREAIRTAVAMLNSGDTLIVAGKGHEIGQEIAGTKLPFSDHEEVRRALKE
RTA
>Mature_483_residues
MKLGEFADILPLSGSIAGETTISGLASDSRRVNAGDLFFALQGSKADGAAYAADAAGRGAVAIIARPGTVGDVGVPVIEA
DDPRHVLALASARLYGAQPATMVAVTGTSGKTSVAAFTRQIWEKAGLAAASIGTTGVVAPGRDEYGELTTPDPVSLHKLL
RELADAGVTHASMEASSHGLDQRRLDGVKLAAGGFTNLGRDHMDYHPTVEHYHQSKMRLFKTLLPKGAPAVIFADDPWSE
PTEKVALAAGLQLLTVGRRGTFLTIKRVEHERHRQRTEIEHEGMIYDIMLPLAGDFQVGNALVAAGLAISTGLPADKAVA
ALEHLKGASGRLELTGTTEDGAQIYVDYAHKPDALENVLQAVRPFTTGRVIVVFGCGGDRDPGKRPIMGEIARRLADVVI
VTDDNPRSEVPAEIRRAILEAVPEATEIGDRREAIRTAVAMLNSGDTLIVAGKGHEIGQEIAGTKLPFSDHEEVRRALKE
RTA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=468, Percent_Identity=37.1794871794872, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1786274, Length=378, Percent_Identity=25.3968253968254, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_MESSB (Q11GS0)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674570.1
- ProteinModelPortal:   Q11GS0
- SMR:   Q11GS0
- STRING:   Q11GS0
- GeneID:   4182805
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_2012
- NMPDR:   fig|266779.1.peg.607
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   DGREHIA
- PhylomeDB:   Q11GS0
- ProtClustDB:   PRK00139
- BioCyc:   MSP266779:MESO_2012-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 51102; Mature: 51102

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 176-176 BINDING 182-182 BINDING 184-184 BINDING 380-380 BINDING 452-452 BINDING 456-456

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGEFADILPLSGSIAGETTISGLASDSRRVNAGDLFFALQGSKADGAAYAADAAGRGA
CCCCCCHHHCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCE
VAIIARPGTVGDVGVPVIEADDPRHVLALASARLYGAQPATMVAVTGTSGKTSVAAFTRQ
EEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
IWEKAGLAAASIGTTGVVAPGRDEYGELTTPDPVSLHKLLRELADAGVTHASMEASSHGL
HHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCC
DQRRLDGVKLAAGGFTNLGRDHMDYHPTVEHYHQSKMRLFKTLLPKGAPAVIFADDPWSE
CHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
PTEKVALAAGLQLLTVGRRGTFLTIKRVEHERHRQRTEIEHEGMIYDIMLPLAGDFQVGN
HHHHHHHHHCCEEEEECCCCCEEEEEHHHHHHHHHHHCHHHCCEEEEEEECCCCCCCCCC
ALVAAGLAISTGLPADKAVAALEHLKGASGRLELTGTTEDGAQIYVDYAHKPDALENVLQ
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHH
AVRPFTTGRVIVVFGCGGDRDPGKRPIMGEIARRLADVVIVTDDNPRSEVPAEIRRAILE
HHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHH
AVPEATEIGDRREAIRTAVAMLNSGDTLIVAGKGHEIGQEIAGTKLPFSDHEEVRRALKE
HCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHH
RTA
CCC
>Mature Secondary Structure
MKLGEFADILPLSGSIAGETTISGLASDSRRVNAGDLFFALQGSKADGAAYAADAAGRGA
CCCCCCHHHCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCE
VAIIARPGTVGDVGVPVIEADDPRHVLALASARLYGAQPATMVAVTGTSGKTSVAAFTRQ
EEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHH
IWEKAGLAAASIGTTGVVAPGRDEYGELTTPDPVSLHKLLRELADAGVTHASMEASSHGL
HHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCC
DQRRLDGVKLAAGGFTNLGRDHMDYHPTVEHYHQSKMRLFKTLLPKGAPAVIFADDPWSE
CHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
PTEKVALAAGLQLLTVGRRGTFLTIKRVEHERHRQRTEIEHEGMIYDIMLPLAGDFQVGN
HHHHHHHHHCCEEEEECCCCCEEEEEHHHHHHHHHHHCHHHCCEEEEEEECCCCCCCCCC
ALVAAGLAISTGLPADKAVAALEHLKGASGRLELTGTTEDGAQIYVDYAHKPDALENVLQ
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHH
AVRPFTTGRVIVVFGCGGDRDPGKRPIMGEIARRLADVVIVTDDNPRSEVPAEIRRAILE
HHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHH
AVPEATEIGDRREAIRTAVAMLNSGDTLIVAGKGHEIGQEIAGTKLPFSDHEEVRRALKE
HCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHH
RTA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA