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The map label for this gene is murF [H]

Identifier: 110634361

GI number: 110634361

Start: 2158643

End: 2160076

Strand: Reverse

Name: murF [H]

Synonym: Meso_2011

Alternate gene names: 110634361

Gene position: 2160076-2158643 (Counterclockwise)

Preceding gene: 110634362

Following gene: 110634360

Centisome position: 48.95

GC content: 63.95

Gene sequence:

>1434_bases
GTGACGCTTCTATGGACTTCGGACGACCTTGTGGCGGCTGTCGGCGGCCGCCCCTTCGGGGCGCTGCCCGAAGGCGTGAC
GGGCATTTCCATCGACAGCAGAACCCTCCAGCGTGGCGAAGCCTTTTTTGCCATCAAGGGTGACCGTTTCGACGGGCACG
ACTTTGCCACCCTGGCCATGGCCGCCGGCGCCGGCCTGCTCGTCATTTCGGAGGAAAAGCTTCCGGCGCTGGGGCGGCTT
ACCGTGCCGAAGGTCGTGGTTCCCGATGTGCTGAAGGCGCTGCGCGACCTTGCCGCCGCCGCCCGCGCGCGCAGCCGGGG
CAAGGTGATCGCCGTCACCGGTTCAGCGGGCAAGACCACCACCAAGGAGATGCTGCGCCACGCTCTCTCGAGCGTGGGGA
CGGTGCATGCCGCCGCCCGCTCCTTCAACAATCATTGGGGTGTGCCCCTCTCGCTCGCCCGTCTGCCTGCCGGTGCAGAT
TTCGCCATTTTCGAGATCGGGATGAACCATGCCGGGGAGATCGAGCCGCTGACGCAGCTCGTAAAACCCCATATAGCGAT
CGTTACGTTGATTGCGGCCGCGCATCTCGGCCATTTTAAAAGCCTGGACGAGATCGCCGAGGCCAAGGGCGAGATCTTTG
CCGGCGTGGTCCCCGGTGGCCACGCGATCATCAACCGGGACGACCAGCGCTTCAAACTTCTTGCCGATATGGCGGCCGAA
GCGGGTATCAGGAATGTCTGGGGCTTCGGCGAGAATGTGCGTTCCCATTTCCGCCTGGCCCAGCAAGAGCCCCAGGGCGA
TCATTCGGTGGTCACGATAAAGGTCGCCGGTCAGGAGCTCATCGGCCGGATCGGCGCGCCCGGGCGGCACATGGTGCAGA
ATGCTCTCTCGGTGATCGGCGCGGGATATCTCGCCGGCGCGGACGTGTCGAAGCTTCTTGCCGCGCTCGGCGATTTCTCT
GCCGAGGCGGGAAGGGGCAAGCTCCATCATCTGGGGATCGGCGAGGGCATGTTCACCCTCATCGATGAAAGCTACAACGC
CAATCCCGCCTCCATGAAGGCAGCCTTGCAGGTGCTCGACAGCTCGCCCGTTTCGAACGGGGGGCGCCGCATCGCCGTGC
TTGGCGATATGCTTGAGCTGGGCGTCCATTCGGAAAAGCTGCACGCGGCGCTTGGCGATATCATCGCCGGAACCAAGACG
GATATCCTCCTTCTGGCAGGGACGGAGATGAAGGCGCTTGCGGACAATGCGCCGCCCGGCCGCCTCGTAGAGTACCGGCC
GACAGTGGAGGAATTGCAGCCGCTTCTCCTTGAGCTGCTGCAGCCCGGAGATGCGATCATGGTGAAGTCGTCGAACGGCA
TCGGCCTGTCCAGGCTGGTCGACGTACTTTTGAAGAATTATCCGGCGGCGGTCGATGAACCGGGCCGGGTTTGA

Upstream 100 bases:

>100_bases
TCGCGGGCAAGGGCCATGAAATCGGCCAGGAAATCGCGGGCACAAAACTCCCGTTTTCCGATCACGAGGAGGTGCGGCGG
GCGCTGAAGGAGAGGACTGC

Downstream 100 bases:

>100_bases
TAGCGCGGACAGGCGCGGGGGCTGTTTTCGATGTTGATGCTGCTGGTTGCACTCGCCGATGAATTCTCGGTGCTAAATGT
TTTTCGCTACATAACCTTTC

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTLLWTSDDLVAAVGGRPFGALPEGVTGISIDSRTLQRGEAFFAIKGDRFDGHDFATLAMAAGAGLLVISEEKLPALGRL
TVPKVVVPDVLKALRDLAAAARARSRGKVIAVTGSAGKTTTKEMLRHALSSVGTVHAAARSFNNHWGVPLSLARLPAGAD
FAIFEIGMNHAGEIEPLTQLVKPHIAIVTLIAAAHLGHFKSLDEIAEAKGEIFAGVVPGGHAIINRDDQRFKLLADMAAE
AGIRNVWGFGENVRSHFRLAQQEPQGDHSVVTIKVAGQELIGRIGAPGRHMVQNALSVIGAGYLAGADVSKLLAALGDFS
AEAGRGKLHHLGIGEGMFTLIDESYNANPASMKAALQVLDSSPVSNGGRRIAVLGDMLELGVHSEKLHAALGDIIAGTKT
DILLLAGTEMKALADNAPPGRLVEYRPTVEELQPLLLELLQPGDAIMVKSSNGIGLSRLVDVLLKNYPAAVDEPGRV

Sequences:

>Translated_477_residues
MTLLWTSDDLVAAVGGRPFGALPEGVTGISIDSRTLQRGEAFFAIKGDRFDGHDFATLAMAAGAGLLVISEEKLPALGRL
TVPKVVVPDVLKALRDLAAAARARSRGKVIAVTGSAGKTTTKEMLRHALSSVGTVHAAARSFNNHWGVPLSLARLPAGAD
FAIFEIGMNHAGEIEPLTQLVKPHIAIVTLIAAAHLGHFKSLDEIAEAKGEIFAGVVPGGHAIINRDDQRFKLLADMAAE
AGIRNVWGFGENVRSHFRLAQQEPQGDHSVVTIKVAGQELIGRIGAPGRHMVQNALSVIGAGYLAGADVSKLLAALGDFS
AEAGRGKLHHLGIGEGMFTLIDESYNANPASMKAALQVLDSSPVSNGGRRIAVLGDMLELGVHSEKLHAALGDIIAGTKT
DILLLAGTEMKALADNAPPGRLVEYRPTVEELQPLLLELLQPGDAIMVKSSNGIGLSRLVDVLLKNYPAAVDEPGRV
>Mature_476_residues
TLLWTSDDLVAAVGGRPFGALPEGVTGISIDSRTLQRGEAFFAIKGDRFDGHDFATLAMAAGAGLLVISEEKLPALGRLT
VPKVVVPDVLKALRDLAAAARARSRGKVIAVTGSAGKTTTKEMLRHALSSVGTVHAAARSFNNHWGVPLSLARLPAGADF
AIFEIGMNHAGEIEPLTQLVKPHIAIVTLIAAAHLGHFKSLDEIAEAKGEIFAGVVPGGHAIINRDDQRFKLLADMAAEA
GIRNVWGFGENVRSHFRLAQQEPQGDHSVVTIKVAGQELIGRIGAPGRHMVQNALSVIGAGYLAGADVSKLLAALGDFSA
EAGRGKLHHLGIGEGMFTLIDESYNANPASMKAALQVLDSSPVSNGGRRIAVLGDMLELGVHSEKLHAALGDIIAGTKTD
ILLLAGTEMKALADNAPPGRLVEYRPTVEELQPLLLELLQPGDAIMVKSSNGIGLSRLVDVLLKNYPAAVDEPGRV

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=440, Percent_Identity=35.4545454545455, Blast_Score=208, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50078; Mature: 49947

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLWTSDDLVAAVGGRPFGALPEGVTGISIDSRTLQRGEAFFAIKGDRFDGHDFATLAM
CEEEEECCCEEEEECCCCCCCCCCCCCEEEECHHHHHCCCEEEEEECCCCCCCHHHHHHH
AAGAGLLVISEEKLPALGRLTVPKVVVPDVLKALRDLAAAARARSRGKVIAVTGSAGKTT
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
TKEMLRHALSSVGTVHAAARSFNNHWGVPLSLARLPAGADFAIFEIGMNHAGEIEPLTQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCCCCEEEEEECCCCCCCHHHHHHH
VKPHIAIVTLIAAAHLGHFKSLDEIAEAKGEIFAGVVPGGHAIINRDDQRFKLLADMAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHH
AGIRNVWGFGENVRSHFRLAQQEPQGDHSVVTIKVAGQELIGRIGAPGRHMVQNALSVIG
CCCHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHH
AGYLAGADVSKLLAALGDFSAEAGRGKLHHLGIGEGMFTLIDESYNANPASMKAALQVLD
CHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCHHEEECCCCCCCHHHHHHHHHHHH
SSPVSNGGRRIAVLGDMLELGVHSEKLHAALGDIIAGTKTDILLLAGTEMKALADNAPPG
CCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCC
RLVEYRPTVEELQPLLLELLQPGDAIMVKSSNGIGLSRLVDVLLKNYPAAVDEPGRV
CEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TLLWTSDDLVAAVGGRPFGALPEGVTGISIDSRTLQRGEAFFAIKGDRFDGHDFATLAM
EEEEECCCEEEEECCCCCCCCCCCCCEEEECHHHHHCCCEEEEEECCCCCCCHHHHHHH
AAGAGLLVISEEKLPALGRLTVPKVVVPDVLKALRDLAAAARARSRGKVIAVTGSAGKTT
HCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHH
TKEMLRHALSSVGTVHAAARSFNNHWGVPLSLARLPAGADFAIFEIGMNHAGEIEPLTQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCCCCEEEEEECCCCCCCHHHHHHH
VKPHIAIVTLIAAAHLGHFKSLDEIAEAKGEIFAGVVPGGHAIINRDDQRFKLLADMAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHH
AGIRNVWGFGENVRSHFRLAQQEPQGDHSVVTIKVAGQELIGRIGAPGRHMVQNALSVIG
CCCHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCHHHHHHHHHHHHH
AGYLAGADVSKLLAALGDFSAEAGRGKLHHLGIGEGMFTLIDESYNANPASMKAALQVLD
CHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCHHEEECCCCCCCHHHHHHHHHHHH
SSPVSNGGRRIAVLGDMLELGVHSEKLHAALGDIIAGTKTDILLLAGTEMKALADNAPPG
CCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCC
RLVEYRPTVEELQPLLLELLQPGDAIMVKSSNGIGLSRLVDVLLKNYPAAVDEPGRV
CEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]