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The map label for this gene is ddl

Identifier: 110634354

GI number: 110634354

Start: 2150149

End: 2151075

Strand: Reverse

Name: ddl

Synonym: Meso_2004

Alternate gene names: 110634354

Gene position: 2151075-2150149 (Counterclockwise)

Preceding gene: 110634355

Following gene: 110634353

Centisome position: 48.75

GC content: 59.01

Gene sequence:

>927_bases
ATGAAGAAAAAGCACGTGGCCGTGCTCATGGGCGGGTTCTCGTCAGAGAGGCCTGTTTCACTGTCTTCAGGCAATTCCTG
CGCCGACGCCCTGGAGGCGGAGGGCTACCGCGTCTCGCGCGTTGACGTCTCGCGTGATGTCAGCCGTGTCCTTTCTGAAT
TGAAGCCCGACGTCGCATTCAATGCGCTGCATGGTCCCTTCGGTGAAGATGGTACCGTTCAGGGCGTATTGGAATATCTG
GGAATTCCCTACACGCATTCGGGCGTGCTCGCTTCGGCTCTCGCCATGGACAAGGACCTGGCGAAGACCGTCGCTAAATC
CGCCGGTATTCCAGTGGCTTCCTCCCGAGTCATGAACCGCTTCGAGATCGGTGATAAACATCCGATGGAGCCGCCTTATG
TCGTCAAGCCGGTAGCCGAGGGGTCAAGCTTCGGCGTGGTGATCGTTCGCGAGGGACAATCGCATCCGCCGCAGGTCCTG
GGCTCTGCCGAATGGGGTTATGGCGAGCGTGTCATGGTCGAGCGCTATATCCCCGGGCGTGAGCTCACCTGCGCGGTCAT
GGGGGACCGGGTGCTCGGGGTCTGCGAGATCGTTCCGGTCGGGCACAGCTTCTATGATTACGATTCGAAATATGCTCCGG
GCGGATCAAGGCATGTTTGTCCGGCGGAAATTTCACCGAATATTTACCAAAAAATAGAAAGACTGGCCCTTAAGGCGCAC
CAAGCGATTGGTTGCCGGGGCGTGAGTCGTTCGGACTTCCGCTATGACGACCGGCTTCCTGGGGAAGAAGGGATCGTCTG
GCTGGAGATCAACACGCAGCCTGGCATGACACCAACCTCCTTGGTGCCGGATATCGCCGCTCAGGCGGGGATTGGCTTTG
GGGCACTCTTGAGTTGGATGGTGGAGGACGCGTCTTGTCCGCGGTGA

Upstream 100 bases:

>100_bases
TTTGAGTCGGCGCGTTTGTAGCCGGGGGCTTTCATCCAAGTGGCAGTTTTGTGTTGCGAGAGCGCTGCGCTTGTCGCGGC
TGGGAAAAGGGCATCGGTGA

Downstream 100 bases:

>100_bases
GCTCAGTGGGGAAGTGGCGCTCCGGCGTTGCCGGCTTCATATTGCCGCGCCTGCTGCGCAGGCCGATGCGCGTGCTTCAG
CGCCTGAGCGAGCGGGAGCT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKKKHVAVLMGGFSSERPVSLSSGNSCADALEAEGYRVSRVDVSRDVSRVLSELKPDVAFNALHGPFGEDGTVQGVLEYL
GIPYTHSGVLASALAMDKDLAKTVAKSAGIPVASSRVMNRFEIGDKHPMEPPYVVKPVAEGSSFGVVIVREGQSHPPQVL
GSAEWGYGERVMVERYIPGRELTCAVMGDRVLGVCEIVPVGHSFYDYDSKYAPGGSRHVCPAEISPNIYQKIERLALKAH
QAIGCRGVSRSDFRYDDRLPGEEGIVWLEINTQPGMTPTSLVPDIAAQAGIGFGALLSWMVEDASCPR

Sequences:

>Translated_308_residues
MKKKHVAVLMGGFSSERPVSLSSGNSCADALEAEGYRVSRVDVSRDVSRVLSELKPDVAFNALHGPFGEDGTVQGVLEYL
GIPYTHSGVLASALAMDKDLAKTVAKSAGIPVASSRVMNRFEIGDKHPMEPPYVVKPVAEGSSFGVVIVREGQSHPPQVL
GSAEWGYGERVMVERYIPGRELTCAVMGDRVLGVCEIVPVGHSFYDYDSKYAPGGSRHVCPAEISPNIYQKIERLALKAH
QAIGCRGVSRSDFRYDDRLPGEEGIVWLEINTQPGMTPTSLVPDIAAQAGIGFGALLSWMVEDASCPR
>Mature_308_residues
MKKKHVAVLMGGFSSERPVSLSSGNSCADALEAEGYRVSRVDVSRDVSRVLSELKPDVAFNALHGPFGEDGTVQGVLEYL
GIPYTHSGVLASALAMDKDLAKTVAKSAGIPVASSRVMNRFEIGDKHPMEPPYVVKPVAEGSSFGVVIVREGQSHPPQVL
GSAEWGYGERVMVERYIPGRELTCAVMGDRVLGVCEIVPVGHSFYDYDSKYAPGGSRHVCPAEISPNIYQKIERLALKAH
QAIGCRGVSRSDFRYDDRLPGEEGIVWLEINTQPGMTPTSLVPDIAAQAGIGFGALLSWMVEDASCPR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=311, Percent_Identity=39.2282958199357, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=31.0924369747899, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_MESSB (Q11GS8)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_674562.1
- ProteinModelPortal:   Q11GS8
- STRING:   Q11GS8
- GeneID:   4182797
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_2004
- NMPDR:   fig|266779.1.peg.599
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- PhylomeDB:   Q11GS8
- ProtClustDB:   PRK01372
- BioCyc:   MSP266779:MESO_2004-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33162; Mature: 33162

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKHVAVLMGGFSSERPVSLSSGNSCADALEAEGYRVSRVDVSRDVSRVLSELKPDVAF
CCCCEEEEEEECCCCCCCEECCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCEE
NALHGPFGEDGTVQGVLEYLGIPYTHSGVLASALAMDKDLAKTVAKSAGIPVASSRVMNR
ECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FEIGDKHPMEPPYVVKPVAEGSSFGVVIVREGQSHPPQVLGSAEWGYGERVMVERYIPGR
HCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHCCCCCCCCCCEEEEEECCCCC
ELTCAVMGDRVLGVCEIVPVGHSFYDYDSKYAPGGSRHVCPAEISPNIYQKIERLALKAH
CEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QAIGCRGVSRSDFRYDDRLPGEEGIVWLEINTQPGMTPTSLVPDIAAQAGIGFGALLSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
VEDASCPR
HHHCCCCC
>Mature Secondary Structure
MKKKHVAVLMGGFSSERPVSLSSGNSCADALEAEGYRVSRVDVSRDVSRVLSELKPDVAF
CCCCEEEEEEECCCCCCCEECCCCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCEE
NALHGPFGEDGTVQGVLEYLGIPYTHSGVLASALAMDKDLAKTVAKSAGIPVASSRVMNR
ECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FEIGDKHPMEPPYVVKPVAEGSSFGVVIVREGQSHPPQVLGSAEWGYGERVMVERYIPGR
HCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHCCCCCCCCCCEEEEEECCCCC
ELTCAVMGDRVLGVCEIVPVGHSFYDYDSKYAPGGSRHVCPAEISPNIYQKIERLALKAH
CEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QAIGCRGVSRSDFRYDDRLPGEEGIVWLEINTQPGMTPTSLVPDIAAQAGIGFGALLSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
VEDASCPR
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA