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The map label for this gene is lutR [H]

Identifier: 110634298

GI number: 110634298

Start: 2088284

End: 2089051

Strand: Reverse

Name: lutR [H]

Synonym: Meso_1948

Alternate gene names: 110634298

Gene position: 2089051-2088284 (Counterclockwise)

Preceding gene: 110634309

Following gene: 110634297

Centisome position: 47.34

GC content: 62.63

Gene sequence:

>768_bases
TTGTGCAATTATCTTGCGGCACACGTGAAGGAGAGCGCATTGGTGGAAATGGTTCCCGCAGGCCGCCCCAGCGGCAACCT
GGTGAAGCATGTCAGCGAGAATCTGCGCAACGAGATCGCTTCCGGCCGATTCGAAGTCGGCGACAAGCTGCCGTCGCAAT
CGCTGCTGACCGAACGCTTCGAGGTAAGCCGCACCGTGATTCGCGAGGCCATTGCCAGCCTGCAAGCCGACGGGCTGCTG
GAAGCGCGCCAGGGTGCCGGGGTTTTCGTCGTCGCGCGCGCCGCGCCATCGCCCTTTCCATTCCAGACCGTCGATCACGC
GCGGGTTTCCTCGATCCTCGAAATGCTCGAACTGCGTTCGGCGATCGAGATCGAGGCCGCCGGCCTAGCCGCTCTGCGCC
GCTCGCCGGCGCAGGAAGAGGCCATTTTCCGCGCCCATCAGGCCGTTGCGGCGCTGATCGCCAACGGCGAACGCTCCACG
GATGCGGACTTCGCCTTCCATCGCGCCATTGCCGGGGCCACCAATAATCCCCGCTTTACCGAGTTTCTCGACCTCCTGGG
CCCCAGCGCCATTCCGCGCGCCAATCTGCAGGTCGATGGCGGCGAAGGTATCCGTGCGCCCTATCTCGAGAAGATTCTCG
ACGAGCATAAACAGATCGCCCAGGCCATTTCATCCGGCGACGAGAAACTGGCGCGCGAGACGATGCGAACTCATCTAAAG
GGCAGTCAGCATCGCTACCGGGCATTAATTCAGAACGACATGGCGTAA

Upstream 100 bases:

>100_bases
ACCTCTTCCTTGGCGACGGAACCTAGTCACCCACGCCGCACTCGTCAACACTTATCATATATCTTTTGCTAAAACACGGT
TTCTTGTCTGAGGACTCGGT

Downstream 100 bases:

>100_bases
CTTGTCATATCTCTATTGACGCTTATCCTATAAATTTGCTAGTGCCCGAACTACTGCGGACGCACAACAGATAAAGGATG
GCGGAATGGAACCCCAGGAT

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MCNYLAAHVKESALVEMVPAGRPSGNLVKHVSENLRNEIASGRFEVGDKLPSQSLLTERFEVSRTVIREAIASLQADGLL
EARQGAGVFVVARAAPSPFPFQTVDHARVSSILEMLELRSAIEIEAAGLAALRRSPAQEEAIFRAHQAVAALIANGERST
DADFAFHRAIAGATNNPRFTEFLDLLGPSAIPRANLQVDGGEGIRAPYLEKILDEHKQIAQAISSGDEKLARETMRTHLK
GSQHRYRALIQNDMA

Sequences:

>Translated_255_residues
MCNYLAAHVKESALVEMVPAGRPSGNLVKHVSENLRNEIASGRFEVGDKLPSQSLLTERFEVSRTVIREAIASLQADGLL
EARQGAGVFVVARAAPSPFPFQTVDHARVSSILEMLELRSAIEIEAAGLAALRRSPAQEEAIFRAHQAVAALIANGERST
DADFAFHRAIAGATNNPRFTEFLDLLGPSAIPRANLQVDGGEGIRAPYLEKILDEHKQIAQAISSGDEKLARETMRTHLK
GSQHRYRALIQNDMA
>Mature_255_residues
MCNYLAAHVKESALVEMVPAGRPSGNLVKHVSENLRNEIASGRFEVGDKLPSQSLLTERFEVSRTVIREAIASLQADGLL
EARQGAGVFVVARAAPSPFPFQTVDHARVSSILEMLELRSAIEIEAAGLAALRRSPAQEEAIFRAHQAVAALIANGERST
DADFAFHRAIAGATNNPRFTEFLDLLGPSAIPRANLQVDGGEGIRAPYLEKILDEHKQIAQAISSGDEKLARETMRTHLK
GSQHRYRALIQNDMA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=222, Percent_Identity=29.7297297297297, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082208, Length=231, Percent_Identity=26.8398268398268, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790780, Length=235, Percent_Identity=29.3617021276596, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786303, Length=216, Percent_Identity=30.0925925925926, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994955, Length=238, Percent_Identity=26.4705882352941, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27740; Mature: 27740

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNYLAAHVKESALVEMVPAGRPSGNLVKHVSENLRNEIASGRFEVGDKLPSQSLLTERF
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHH
EVSRTVIREAIASLQADGLLEARQGAGVFVVARAAPSPFPFQTVDHARVSSILEMLELRS
HHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AIEIEAAGLAALRRSPAQEEAIFRAHQAVAALIANGERSTDADFAFHRAIAGATNNPRFT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHH
EFLDLLGPSAIPRANLQVDGGEGIRAPYLEKILDEHKQIAQAISSGDEKLARETMRTHLK
HHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GSQHRYRALIQNDMA
CCHHHHHHHHHHCCC
>Mature Secondary Structure
MCNYLAAHVKESALVEMVPAGRPSGNLVKHVSENLRNEIASGRFEVGDKLPSQSLLTERF
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHH
EVSRTVIREAIASLQADGLLEARQGAGVFVVARAAPSPFPFQTVDHARVSSILEMLELRS
HHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AIEIEAAGLAALRRSPAQEEAIFRAHQAVAALIANGERSTDADFAFHRAIAGATNNPRFT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHH
EFLDLLGPSAIPRANLQVDGGEGIRAPYLEKILDEHKQIAQAISSGDEKLARETMRTHLK
HHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GSQHRYRALIQNDMA
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]