| Definition | Mesorhizobium sp. BNC1, complete genome. |
|---|---|
| Accession | NC_008254 |
| Length | 4,412,446 |
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The map label for this gene is mrcA [H]
Identifier: 110634060
GI number: 110634060
Start: 1829401
End: 1831857
Strand: Reverse
Name: mrcA [H]
Synonym: Meso_1708
Alternate gene names: 110634060
Gene position: 1831857-1829401 (Counterclockwise)
Preceding gene: 110634061
Following gene: 110634059
Centisome position: 41.52
GC content: 60.77
Gene sequence:
>2457_bases ATGTTACGTCTCTTAGGATATTTTTTCGGCATCGGCATTGTCTTTGCGCTGATTGCTGCTGCAGGTGTGGCACTCTATGT CGGAGATCTGTCGAAAGACCTGCCTGATTACGAAGTGCTGGCAAAGTATGAACCGCCGGTGATGACCCGCGTTCATGCGT CCGACGGTTCGCTGATGGCGGAATTCGCCCGCGAGCGGCGCCTTTACCTTCCGATCAAGGCCATTCCGGACCGTGTAAAG GCCGCATTTCTTTCGGCTGAGGACAAGAGCTTCTATTCCCACCCTGGCGTTGATCCGGCGGGTCTGGTCAGCGCTGTGTT CACCAATCTGCAGAATATCGGTACCGGGCGCAGGCCGGTCGGTGCATCCACGATCACGCAGCAGGTGGCAAAGAATTTCC TGCTGAGCTCTGACCAGACGATCGAGCGGAAGATTAAGGAGATGATTCTCGCCTTCCGTATCGAGCAAGCCTACTCCAAG GATCGCATCCTGGAGCTTTATTTGAATGAGATCTTCTTCGGTCTTGGCGCATACGGAATCGCCGGCGCGGCATTGGCCTA TTACGACAAGCCTGTTTCAGAACTCAGCATAGCGGAAGCCGCATACCTCGCCTCGCTGCCCAAAGGGCCTTCGAACTACC ATCCTTTCCGTCATACCGATCGCGCCCTCGAGCGCCGCAATTGGGTAATCGATCGGATGCACGCGAACGGCTACATCACC GCCAAGGAAGCCGCGGAGGCGAAGGCCACCGGCCTCGACGTGAAGCCGCGCGAACGTGGCAATTATCTCGCCGGCTCTGA ATATTTCACCGAGGAAGTCCGGCGTGAGCTTATCGCGCGCTATGGAGAGGACGCCCTTTATGATGGCGGCCTCTATGTAC GCACTACGCTCGATCCAAAGCTGCAGCTTCTCGCTGAGAAAGCCTTGCGCCACGGCCTGATGAAGTACGATACCCTGCGG GGCTACCGCGGGCCGGTGAAGCATATCGATATCTCCGGCGATTGGGGCGAACCGCTGGCCGAAGTGCCCGGTCTGCGTGA CGTATCCGAGTGGGCTTTGGCCGTTGTCCTTGAAGCTTCCGGCAACGATGTGAAGATCGGGATACAGCCCAAGCGCGAGA TCTCCGGCGAGCTGTCGAAGGAGCGGGAAACCGGAACAATCGACACTGGCGATATCGGCTGGGTGCTCCGTCACGTGGTC GATGGGAAGCTCCTCAAGGCAAAGTCGCTGGAAGACGTGTTCGAGCCGGGCGATGTGATCTATGTCGAGCGCAAAAGCGA GGGACAGGATACGTGGCTGGTTCGGCAGGTGCCGGCAATCGGCGGCGCGCTGGTTGCCATGGATCCGCACACCGGCCGCG TGCTTGCAATGGCGGGAGGCTTCTCTTACGCGGCCTCCGAGTTCAACCGCGCCACGCAGGCCTACCGCCAGCCCGGCTCC TCCTTCAAGCCCATCATATATGCCGCGGCACTCGACAACGGATACACGCCCGCGACGGTGATCATGGACGCGCCGCTCAC CATCCATGTCGGAAACCAGGTCTGGGAGCCGAAGAACTATGGCGGCGATTTCGCCGGCCCCTCCACGCTGCGCACAGGCA TCGAGCGCTCGCGTAACCTGATGACGGTTCGGCTAGCGCAGGATATGGGAATGGATCTTGTCGCCGAATATGCGGAGCGT TTCGGCATCTATGACGACATGCCGGAGCACATCGCCATGTCGCTGGGCTCGGGCGAGACGACGGTGATGCGCATGGTCTC GGCCTATGCGGTCATGGCCAATGGCGGCAAGTTCATCCAGCCCTCGCTCGTCGATCGCATCCAAGACCGCTACGGCAGGA CCGTCTACAAACACGACACGCGCGAGTGCGAGGGCTGTGCCGCCGATGCTTGGAAAAATCAGCCCGAAGCCGAGCTGATC GACAATCGCGAGCAGGTGCTTGACCCGATGACCGCGTACCAGATCACCTCCATCATGCAGGGCGTGGTGCAGCGCGGCAC GGCAAGCAGCCTGGCGGAGCTCGGCCGCCCCATTGCGGGCAAAACCGGTACGACGAACGACGAGAAGGATGCCTGGTTCA TTGGCTATACGCCGGACATGGTCGTAGGCGTTTTCATGGGATACGATCAGCCGAAGCCCATGGGCAAGGGTTCCACCGGC AGCGGCCTGGCGGCACCGGTCTTCAAGGAATTCATGGCGGATGCCCTGAAGGACATGCGTCCCGTGGACTTCCAGGTTCC CGAAGGCATGCAGCTCATCGCCATCGACCGGAAGACCGGCATGCGGGCTCAGGAAGGTGCGCCCGGCACCATCCTGGAAG CCTTCAAGCCCGGCACCGGCCCGGCGGACAGCTATTGGGTCATCGGCATGGAGAGCTCCCAGGTGAGCACCGGCGCGAGC AATATCTCGCCCGAGGCAAACCGGGCGATCACCTCCGGGGCAGGCGGCCTCTTTTAG
Upstream 100 bases:
>100_bases GGCAGGTGATGCGCATCGTCGTGATTTCACCTCCGGGCGATTATAGTGCTATCATTGCCGGGGGAGGAGTAGCCGGGCTA CCGGTCACACTGGAGCGAGC
Downstream 100 bases:
>100_bases CCCTTTGCAAAGCCCCAAAGCGGTCCTATCGCAGATGGGGCCGCTTTTTCTTTACACGCGGTGCTTCGATACATAAGTTC CGCCGCGAGTCGGGTGACGA
Product: 1A family penicillin-binding protein
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 818; Mature: 818
Protein sequence:
>818_residues MLRLLGYFFGIGIVFALIAAAGVALYVGDLSKDLPDYEVLAKYEPPVMTRVHASDGSLMAEFARERRLYLPIKAIPDRVK AAFLSAEDKSFYSHPGVDPAGLVSAVFTNLQNIGTGRRPVGASTITQQVAKNFLLSSDQTIERKIKEMILAFRIEQAYSK DRILELYLNEIFFGLGAYGIAGAALAYYDKPVSELSIAEAAYLASLPKGPSNYHPFRHTDRALERRNWVIDRMHANGYIT AKEAAEAKATGLDVKPRERGNYLAGSEYFTEEVRRELIARYGEDALYDGGLYVRTTLDPKLQLLAEKALRHGLMKYDTLR GYRGPVKHIDISGDWGEPLAEVPGLRDVSEWALAVVLEASGNDVKIGIQPKREISGELSKERETGTIDTGDIGWVLRHVV DGKLLKAKSLEDVFEPGDVIYVERKSEGQDTWLVRQVPAIGGALVAMDPHTGRVLAMAGGFSYAASEFNRATQAYRQPGS SFKPIIYAAALDNGYTPATVIMDAPLTIHVGNQVWEPKNYGGDFAGPSTLRTGIERSRNLMTVRLAQDMGMDLVAEYAER FGIYDDMPEHIAMSLGSGETTVMRMVSAYAVMANGGKFIQPSLVDRIQDRYGRTVYKHDTRECEGCAADAWKNQPEAELI DNREQVLDPMTAYQITSIMQGVVQRGTASSLAELGRPIAGKTGTTNDEKDAWFIGYTPDMVVGVFMGYDQPKPMGKGSTG SGLAAPVFKEFMADALKDMRPVDFQVPEGMQLIAIDRKTGMRAQEGAPGTILEAFKPGTGPADSYWVIGMESSQVSTGAS NISPEANRAITSGAGGLF
Sequences:
>Translated_818_residues MLRLLGYFFGIGIVFALIAAAGVALYVGDLSKDLPDYEVLAKYEPPVMTRVHASDGSLMAEFARERRLYLPIKAIPDRVK AAFLSAEDKSFYSHPGVDPAGLVSAVFTNLQNIGTGRRPVGASTITQQVAKNFLLSSDQTIERKIKEMILAFRIEQAYSK DRILELYLNEIFFGLGAYGIAGAALAYYDKPVSELSIAEAAYLASLPKGPSNYHPFRHTDRALERRNWVIDRMHANGYIT AKEAAEAKATGLDVKPRERGNYLAGSEYFTEEVRRELIARYGEDALYDGGLYVRTTLDPKLQLLAEKALRHGLMKYDTLR GYRGPVKHIDISGDWGEPLAEVPGLRDVSEWALAVVLEASGNDVKIGIQPKREISGELSKERETGTIDTGDIGWVLRHVV DGKLLKAKSLEDVFEPGDVIYVERKSEGQDTWLVRQVPAIGGALVAMDPHTGRVLAMAGGFSYAASEFNRATQAYRQPGS SFKPIIYAAALDNGYTPATVIMDAPLTIHVGNQVWEPKNYGGDFAGPSTLRTGIERSRNLMTVRLAQDMGMDLVAEYAER FGIYDDMPEHIAMSLGSGETTVMRMVSAYAVMANGGKFIQPSLVDRIQDRYGRTVYKHDTRECEGCAADAWKNQPEAELI DNREQVLDPMTAYQITSIMQGVVQRGTASSLAELGRPIAGKTGTTNDEKDAWFIGYTPDMVVGVFMGYDQPKPMGKGSTG SGLAAPVFKEFMADALKDMRPVDFQVPEGMQLIAIDRKTGMRAQEGAPGTILEAFKPGTGPADSYWVIGMESSQVSTGAS NISPEANRAITSGAGGLF >Mature_818_residues MLRLLGYFFGIGIVFALIAAAGVALYVGDLSKDLPDYEVLAKYEPPVMTRVHASDGSLMAEFARERRLYLPIKAIPDRVK AAFLSAEDKSFYSHPGVDPAGLVSAVFTNLQNIGTGRRPVGASTITQQVAKNFLLSSDQTIERKIKEMILAFRIEQAYSK DRILELYLNEIFFGLGAYGIAGAALAYYDKPVSELSIAEAAYLASLPKGPSNYHPFRHTDRALERRNWVIDRMHANGYIT AKEAAEAKATGLDVKPRERGNYLAGSEYFTEEVRRELIARYGEDALYDGGLYVRTTLDPKLQLLAEKALRHGLMKYDTLR GYRGPVKHIDISGDWGEPLAEVPGLRDVSEWALAVVLEASGNDVKIGIQPKREISGELSKERETGTIDTGDIGWVLRHVV DGKLLKAKSLEDVFEPGDVIYVERKSEGQDTWLVRQVPAIGGALVAMDPHTGRVLAMAGGFSYAASEFNRATQAYRQPGS SFKPIIYAAALDNGYTPATVIMDAPLTIHVGNQVWEPKNYGGDFAGPSTLRTGIERSRNLMTVRLAQDMGMDLVAEYAER FGIYDDMPEHIAMSLGSGETTVMRMVSAYAVMANGGKFIQPSLVDRIQDRYGRTVYKHDTRECEGCAADAWKNQPEAELI DNREQVLDPMTAYQITSIMQGVVQRGTASSLAELGRPIAGKTGTTNDEKDAWFIGYTPDMVVGVFMGYDQPKPMGKGSTG SGLAAPVFKEFMADALKDMRPVDFQVPEGMQLIAIDRKTGMRAQEGAPGTILEAFKPGTGPADSYWVIGMESSQVSTGAS NISPEANRAITSGAGGLF
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=866, Percent_Identity=31.986143187067, Blast_Score=414, Evalue=1e-116, Organism=Escherichia coli, GI1786343, Length=276, Percent_Identity=35.8695652173913, Blast_Score=150, Evalue=5e-37, Organism=Escherichia coli, GI1788867, Length=252, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=1e-26, Organism=Escherichia coli, GI1789601, Length=169, Percent_Identity=35.5029585798817, Blast_Score=94, Evalue=3e-20,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.-
Molecular weight: Translated: 89430; Mature: 89430
Theoretical pI: Translated: 5.42; Mature: 5.42
Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 3.3 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 3.3 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLRLLGYFFGIGIVFALIAAAGVALYVGDLSKDLPDYEVLAKYEPPVMTRVHASDGSLMA CHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCCCHHHHCCCCCCCEEEEECCCCHHHH EFARERRLYLPIKAIPDRVKAAFLSAEDKSFYSHPGVDPAGLVSAVFTNLQNIGTGRRPV HHHHCCEEEEEHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC GASTITQQVAKNFLLSSDQTIERKIKEMILAFRIEQAYSKDRILELYLNEIFFGLGAYGI CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH AGAALAYYDKPVSELSIAEAAYLASLPKGPSNYHPFRHTDRALERRNWVIDRMHANGYIT HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCEEE AKEAAEAKATGLDVKPRERGNYLAGSEYFTEEVRRELIARYGEDALYDGGLYVRTTLDPK HHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCHH LQLLAEKALRHGLMKYDTLRGYRGPVKHIDISGDWGEPLAEVPGLRDVSEWALAVVLEAS HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHCCCCCCHHHEEEEEEEECC GNDVKIGIQPKREISGELSKERETGTIDTGDIGWVLRHVVDGKLLKAKSLEDVFEPGDVI CCEEEEECCCCHHHCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCEE YVERKSEGQDTWLVRQVPAIGGALVAMDPHTGRVLAMAGGFSYAASEFNRATQAYRQPGS EEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC SFKPIIYAAALDNGYTPATVIMDAPLTIHVGNQVWEPKNYGGDFAGPSTLRTGIERSRNL CCCCEEEEEECCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCE MTVRLAQDMGMDLVAEYAERFGIYDDMPEHIAMSLGSGETTVMRMVSAYAVMANGGKFIQ EEEEEHHHCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHEECCCCEEC PSLVDRIQDRYGRTVYKHDTRECEGCAADAWKNQPEAELIDNREQVLDPMTAYQITSIMQ HHHHHHHHHHHCCCEEECCCHHHCCCHHHHHCCCCCHHHHCCHHHHHCHHHHHHHHHHHH GVVQRGTASSLAELGRPIAGKTGTTNDEKDAWFIGYTPDMVVGVFMGYDQPKPMGKGSTG HHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCC SGLAAPVFKEFMADALKDMRPVDFQVPEGMQLIAIDRKTGMRAQEGAPGTILEAFKPGTG CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCC PADSYWVIGMESSQVSTGASNISPEANRAITSGAGGLF CCCCEEEEEECCCCCCCCCCCCCCCHHHHEECCCCCCC >Mature Secondary Structure MLRLLGYFFGIGIVFALIAAAGVALYVGDLSKDLPDYEVLAKYEPPVMTRVHASDGSLMA CHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCCCHHHHCCCCCCCEEEEECCCCHHHH EFARERRLYLPIKAIPDRVKAAFLSAEDKSFYSHPGVDPAGLVSAVFTNLQNIGTGRRPV HHHHCCEEEEEHHHCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC GASTITQQVAKNFLLSSDQTIERKIKEMILAFRIEQAYSKDRILELYLNEIFFGLGAYGI CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH AGAALAYYDKPVSELSIAEAAYLASLPKGPSNYHPFRHTDRALERRNWVIDRMHANGYIT HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCEEE AKEAAEAKATGLDVKPRERGNYLAGSEYFTEEVRRELIARYGEDALYDGGLYVRTTLDPK HHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCEEEEEECCHH LQLLAEKALRHGLMKYDTLRGYRGPVKHIDISGDWGEPLAEVPGLRDVSEWALAVVLEAS HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHCCCCCCHHHEEEEEEEECC GNDVKIGIQPKREISGELSKERETGTIDTGDIGWVLRHVVDGKLLKAKSLEDVFEPGDVI CCEEEEECCCCHHHCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHCCCCEE YVERKSEGQDTWLVRQVPAIGGALVAMDPHTGRVLAMAGGFSYAASEFNRATQAYRQPGS EEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCC SFKPIIYAAALDNGYTPATVIMDAPLTIHVGNQVWEPKNYGGDFAGPSTLRTGIERSRNL CCCCEEEEEECCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCE MTVRLAQDMGMDLVAEYAERFGIYDDMPEHIAMSLGSGETTVMRMVSAYAVMANGGKFIQ EEEEEHHHCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHEECCCCEEC PSLVDRIQDRYGRTVYKHDTRECEGCAADAWKNQPEAELIDNREQVLDPMTAYQITSIMQ HHHHHHHHHHHCCCEEECCCHHHCCCHHHHHCCCCCHHHHCCHHHHHCHHHHHHHHHHHH GVVQRGTASSLAELGRPIAGKTGTTNDEKDAWFIGYTPDMVVGVFMGYDQPKPMGKGSTG HHHHCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCC SGLAAPVFKEFMADALKDMRPVDFQVPEGMQLIAIDRKTGMRAQEGAPGTILEAFKPGTG CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCC PADSYWVIGMESSQVSTGASNISPEANRAITSGAGGLF CCCCEEEEEECCCCCCCCCCCCCCCHHHHEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA