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The map label for this gene is amiC [H]

Identifier: 110634061

GI number: 110634061

Start: 1832052

End: 1833311

Strand: Reverse

Name: amiC [H]

Synonym: Meso_1709

Alternate gene names: 110634061

Gene position: 1833311-1832052 (Counterclockwise)

Preceding gene: 110634062

Following gene: 110634060

Centisome position: 41.55

GC content: 60.4

Gene sequence:

>1260_bases
GTGGCGGAACGGCCGACTCCAAAAGCCATGCGGGCGCATCGCGCGAGGCGAGAAACACGGCACGGACCGCATGCTTCCGG
CCCTTTTCTTCTCTTGCTTTTCCTTCTGACGGCCTTCTGGGGGCCACCAGCATTCGCCCAAGCGGTTCAACCGCTTGTCG
CCTATGATCATACGATGGCCGGTGATGCGAACCGTGTGCGGATCGTACTCAACTTCGACCGGAAACCGGAAGTCAACTGG
TTTCTCCTGCGCGCGCCGCACCGGCTGGTGGTGGATTTTCCCGAAACGGATTTCGGCATCGAGGAGAAGGAGACCGAGCC
CCGGGGGCTGATCTCACGCGTTCGCTATGGCCGGATGGCGCCTGGCCATTCGCGGATGATCTACACCATGCCGGGCCCAT
TCAAGGTCGAGGAGGTCTCGGTGCTGAAAAACGAGACCTCGCCGGGTTACCGGATGATCGCCGATATCGTTTCAGCTTCG
GAGAGCGATTTCGAATCGGCAATGCGCGAGCGCCTTGCCGCTGCGCCAGATGCAGGAACTGCAAAGGAAGTGGTGAAGCA
AAAGCCGACGGATGACCGCTTCATGATAGCGATCGATCCCGGCCACGGCGGAATCGACGGGGGAGCGCGCGGCGTGAGCG
GCACCTTCGAAAAGACGATTACCCTTACTTTTGCTCAGGAACTCAAGAAGAGCCTCGAGGCAACCGGCAAATACAATGTC
GTGCTTACGAGAAACGAAGACGTTTTCCTGCGGCTCGACGAGCGGGTGCGAATTGCGCGCGAGAACGAGGCCGACCTTCT
CATTTCCATCCATGCCGATGCGATTTCAATGCGCGATTTCCGCGGCGCCACGGTTTATACCTTGTCGGATCGGGCCTCCG
ACGCGGAGGCGGCGGCAACGGCTGCACGGGAAAACCTCTCCGACGAGCTCGCGGGCTTGACGGCAGAGGAGGAGCAGGAC
CATGTCGCCGACATTCTATTCGACCTCATTCGCCGGGAGACGCACGCCTTCTCCATCCATTTTGCCCGCACCTTGCTCGA
CAGCCTCGGCGAGACGGTGCACCTGGTCGGCAATCCCCTGCGTTCAGCAGGTTTTCTCGTTCTCAAGGCGCCGGATGTGC
CCTCCGTTCTCGTTGAGCTCGGCTATCTGTCAAATCCGGAAGACGAGAAACAGATGAAGGACCCGGCCTGGCGCGCGAAG
GCAGTCGATTCGATCCTTCGGGCGATCAACATATTCGCTGCCGCGAGGGCGGGTGGATAA

Upstream 100 bases:

>100_bases
GCCCGGTCCGATCGCGCAGAACGGAACCTGGTTGCCTTGAAGCGGCCCAAAGCTTCGTTTAACGGCGCGTAACGGTTGCC
AAATCGAAGTGGGGACTTAC

Downstream 100 bases:

>100_bases
GCCGATCCGTTGCAATCTCACAACGCTCTCGCTTCTTTTTGCGTTGGCAAACGATAAAGGCTGGCGGGCTGCCTAATTTT
GCTCACAAGACGCAGGCAGG

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MAERPTPKAMRAHRARRETRHGPHASGPFLLLLFLLTAFWGPPAFAQAVQPLVAYDHTMAGDANRVRIVLNFDRKPEVNW
FLLRAPHRLVVDFPETDFGIEEKETEPRGLISRVRYGRMAPGHSRMIYTMPGPFKVEEVSVLKNETSPGYRMIADIVSAS
ESDFESAMRERLAAAPDAGTAKEVVKQKPTDDRFMIAIDPGHGGIDGGARGVSGTFEKTITLTFAQELKKSLEATGKYNV
VLTRNEDVFLRLDERVRIARENEADLLISIHADAISMRDFRGATVYTLSDRASDAEAAATAARENLSDELAGLTAEEEQD
HVADILFDLIRRETHAFSIHFARTLLDSLGETVHLVGNPLRSAGFLVLKAPDVPSVLVELGYLSNPEDEKQMKDPAWRAK
AVDSILRAINIFAAARAGG

Sequences:

>Translated_419_residues
MAERPTPKAMRAHRARRETRHGPHASGPFLLLLFLLTAFWGPPAFAQAVQPLVAYDHTMAGDANRVRIVLNFDRKPEVNW
FLLRAPHRLVVDFPETDFGIEEKETEPRGLISRVRYGRMAPGHSRMIYTMPGPFKVEEVSVLKNETSPGYRMIADIVSAS
ESDFESAMRERLAAAPDAGTAKEVVKQKPTDDRFMIAIDPGHGGIDGGARGVSGTFEKTITLTFAQELKKSLEATGKYNV
VLTRNEDVFLRLDERVRIARENEADLLISIHADAISMRDFRGATVYTLSDRASDAEAAATAARENLSDELAGLTAEEEQD
HVADILFDLIRRETHAFSIHFARTLLDSLGETVHLVGNPLRSAGFLVLKAPDVPSVLVELGYLSNPEDEKQMKDPAWRAK
AVDSILRAINIFAAARAGG
>Mature_418_residues
AERPTPKAMRAHRARRETRHGPHASGPFLLLLFLLTAFWGPPAFAQAVQPLVAYDHTMAGDANRVRIVLNFDRKPEVNWF
LLRAPHRLVVDFPETDFGIEEKETEPRGLISRVRYGRMAPGHSRMIYTMPGPFKVEEVSVLKNETSPGYRMIADIVSASE
SDFESAMRERLAAAPDAGTAKEVVKQKPTDDRFMIAIDPGHGGIDGGARGVSGTFEKTITLTFAQELKKSLEATGKYNVV
LTRNEDVFLRLDERVRIARENEADLLISIHADAISMRDFRGATVYTLSDRASDAEAAATAARENLSDELAGLTAEEEQDH
VADILFDLIRRETHAFSIHFARTLLDSLGETVHLVGNPLRSAGFLVLKAPDVPSVLVELGYLSNPEDEKQMKDPAWRAKA
VDSILRAINIFAAARAGG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=370, Percent_Identity=31.3513513513514, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1788776, Length=225, Percent_Identity=37.7777777777778, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1790611, Length=231, Percent_Identity=32.4675324675325, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46323; Mature: 46192

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERPTPKAMRAHRARRETRHGPHASGPFLLLLFLLTAFWGPPAFAQAVQPLVAYDHTMA
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
GDANRVRIVLNFDRKPEVNWFLLRAPHRLVVDFPETDFGIEEKETEPRGLISRVRYGRMA
CCCCEEEEEEECCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
PGHSRMIYTMPGPFKVEEVSVLKNETSPGYRMIADIVSASESDFESAMRERLAAAPDAGT
CCCCEEEEECCCCCEEHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
AKEVVKQKPTDDRFMIAIDPGHGGIDGGARGVSGTFEKTITLTFAQELKKSLEATGKYNV
HHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHCCCCEEE
VLTRNEDVFLRLDERVRIARENEADLLISIHADAISMRDFRGATVYTLSDRASDAEAAAT
EEEECCCEEEEECCCEEEECCCCCCEEEEEECCEEEHHCCCCCEEEEECCCCCCHHHHHH
AARENLSDELAGLTAEEEQDHVADILFDLIRRETHAFSIHFARTLLDSLGETVHLVGNPL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
RSAGFLVLKAPDVPSVLVELGYLSNPEDEKQMKDPAWRAKAVDSILRAINIFAAARAGG
CCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AERPTPKAMRAHRARRETRHGPHASGPFLLLLFLLTAFWGPPAFAQAVQPLVAYDHTMA
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
GDANRVRIVLNFDRKPEVNWFLLRAPHRLVVDFPETDFGIEEKETEPRGLISRVRYGRMA
CCCCEEEEEEECCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
PGHSRMIYTMPGPFKVEEVSVLKNETSPGYRMIADIVSASESDFESAMRERLAAAPDAGT
CCCCEEEEECCCCCEEHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
AKEVVKQKPTDDRFMIAIDPGHGGIDGGARGVSGTFEKTITLTFAQELKKSLEATGKYNV
HHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHHHCCCCEEE
VLTRNEDVFLRLDERVRIARENEADLLISIHADAISMRDFRGATVYTLSDRASDAEAAAT
EEEECCCEEEEECCCEEEECCCCCCEEEEEECCEEEHHCCCCCEEEEECCCCCCHHHHHH
AARENLSDELAGLTAEEEQDHVADILFDLIRRETHAFSIHFARTLLDSLGETVHLVGNPL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCC
RSAGFLVLKAPDVPSVLVELGYLSNPEDEKQMKDPAWRAKAVDSILRAINIFAAARAGG
CCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]