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The map label for this gene is xthA [H]

Identifier: 110634038

GI number: 110634038

Start: 1802583

End: 1803371

Strand: Direct

Name: xthA [H]

Synonym: Meso_1686

Alternate gene names: 110634038

Gene position: 1802583-1803371 (Clockwise)

Preceding gene: 110634004

Following gene: 110634048

Centisome position: 40.85

GC content: 61.47

Gene sequence:

>789_bases
ATGAAAATCGCCACCTGGAACATCAATGGCGTCAAGGCGCGCATCGAAAACCTCGTGCACTGGCTGCAGGAGGCCAATCC
GGACATCGTGTGCCTTCAGGAGATCAAGTCTCTTGACGAGCAGTTCCCTCGTGAGCCGATTGAGGCACTGGGTTATCATG
TTGAAACCCACGGGCAGAAGGGTTTCAACGGCGTCGCCCTTCTCTCAAAACTCAGATTCGACGAGGTCAGCCGCGGCCTG
CCGGGCAATGACGGCGACGAACAGGCGCGCTTCATTGAGGGTGTGTTCTCAACCGAAGGCGGTGCGCTGCGCGTGGCATC
GCTCTACCTGCCCAACGGCAATCCCATCGCCAGCGAGAAATTCGCCTATAAGCTCGCCTGGATGGACCGGCTGGAGCTTT
GGGCGAAGCGCCGGCTGATGCTGGAAGAGCCTCTGGTGCTGGCCGGTGATTATAATGTGATTCCCGAACCGGCGGATGCC
AAGAACCCTGAGAACTGGCTGGGGGACGCGCTCTACCAGCCGGAGAGCCGGCAGGCCTTCCGCCGCCTCTGCAATCTGGG
CTTCACCGACGCGCTGCGCTCCGCAACCAACGAGCCGGGCACATATACGTTCTGGGACTATCAGGCAGGGGCATGGCAGA
AGAACAACGGCATCCGGATCGACCACCTGATGCTGTCGCCGGAAGCTGCCGACCGGATCGCCTCCGCTTCCGTCCAGAAG
CACACGCGCGCATGGGAAAAGCCGTCGGACCACGTTCCGGTGGTGGTGGACCTGCGAATCGCGGCCTAG

Upstream 100 bases:

>100_bases
TTGTTCCTTGGGAACTTGCCGACTACGGCATCGCCGCTTTGACGGGCGGCCCAATTGAGCTAGTGTGCTGCTCCCGCGGT
GCAGTTCTGGATTCGTGCTC

Downstream 100 bases:

>100_bases
AATTGCTTCGAGAAGCCGGTGGCTGCCTACTCTAGCCCGTTCGCCAAGTAGTCTTGAGCAAGCGCAATTGCGGTGCGGCG
ATCCGATTCGCCCGCAAGTG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKIATWNINGVKARIENLVHWLQEANPDIVCLQEIKSLDEQFPREPIEALGYHVETHGQKGFNGVALLSKLRFDEVSRGL
PGNDGDEQARFIEGVFSTEGGALRVASLYLPNGNPIASEKFAYKLAWMDRLELWAKRRLMLEEPLVLAGDYNVIPEPADA
KNPENWLGDALYQPESRQAFRRLCNLGFTDALRSATNEPGTYTFWDYQAGAWQKNNGIRIDHLMLSPEAADRIASASVQK
HTRAWEKPSDHVPVVVDLRIAA

Sequences:

>Translated_262_residues
MKIATWNINGVKARIENLVHWLQEANPDIVCLQEIKSLDEQFPREPIEALGYHVETHGQKGFNGVALLSKLRFDEVSRGL
PGNDGDEQARFIEGVFSTEGGALRVASLYLPNGNPIASEKFAYKLAWMDRLELWAKRRLMLEEPLVLAGDYNVIPEPADA
KNPENWLGDALYQPESRQAFRRLCNLGFTDALRSATNEPGTYTFWDYQAGAWQKNNGIRIDHLMLSPEAADRIASASVQK
HTRAWEKPSDHVPVVVDLRIAA
>Mature_262_residues
MKIATWNINGVKARIENLVHWLQEANPDIVCLQEIKSLDEQFPREPIEALGYHVETHGQKGFNGVALLSKLRFDEVSRGL
PGNDGDEQARFIEGVFSTEGGALRVASLYLPNGNPIASEKFAYKLAWMDRLELWAKRRLMLEEPLVLAGDYNVIPEPADA
KNPENWLGDALYQPESRQAFRRLCNLGFTDALRSATNEPGTYTFWDYQAGAWQKNNGIRIDHLMLSPEAADRIASASVQK
HTRAWEKPSDHVPVVVDLRIAA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=30.7116104868914, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=30.7116104868914, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=30.7116104868914, Blast_Score=118, Evalue=5e-27,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=34.8148148148148, Blast_Score=153, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71989536, Length=271, Percent_Identity=28.0442804428044, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=30.0751879699248, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=30.0751879699248, Blast_Score=111, Evalue=4e-25,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29494; Mature: 29494

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNINGVKARIENLVHWLQEANPDIVCLQEIKSLDEQFPREPIEALGYHVETHGQK
CEEEEECCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCHHHHHCEEEECCCCC
GFNGVALLSKLRFDEVSRGLPGNDGDEQARFIEGVFSTEGGALRVASLYLPNGNPIASEK
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHH
FAYKLAWMDRLELWAKRRLMLEEPLVLAGDYNVIPEPADAKNPENWLGDALYQPESRQAF
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
RRLCNLGFTDALRSATNEPGTYTFWDYQAGAWQKNNGIRIDHLMLSPEAADRIASASVQK
HHHHCCCHHHHHHHHCCCCCCEEEEECCCCCEECCCCEEEEEEEECCHHHHHHHHHHHHH
HTRAWEKPSDHVPVVVDLRIAA
HHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKIATWNINGVKARIENLVHWLQEANPDIVCLQEIKSLDEQFPREPIEALGYHVETHGQK
CEEEEECCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCHHHHHCEEEECCCCC
GFNGVALLSKLRFDEVSRGLPGNDGDEQARFIEGVFSTEGGALRVASLYLPNGNPIASEK
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHH
FAYKLAWMDRLELWAKRRLMLEEPLVLAGDYNVIPEPADAKNPENWLGDALYQPESRQAF
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
RRLCNLGFTDALRSATNEPGTYTFWDYQAGAWQKNNGIRIDHLMLSPEAADRIASASVQK
HHHHCCCHHHHHHHHCCCCCCEEEEECCCCCEECCCCEEEEEEEECCHHHHHHHHHHHHH
HTRAWEKPSDHVPVVVDLRIAA
HHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]