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The map label for this gene is pdhB [H]

Identifier: 110633981

GI number: 110633981

Start: 1754432

End: 1755832

Strand: Reverse

Name: pdhB [H]

Synonym: Meso_1629

Alternate gene names: 110633981

Gene position: 1755832-1754432 (Counterclockwise)

Preceding gene: 110633982

Following gene: 110633980

Centisome position: 39.79

GC content: 60.53

Gene sequence:

>1401_bases
ATGCCCACGCAGATACTCATGCCCGCTCTCTCTCCGACCATGGAAGAGGGCAACCTCTCAAAATGGGTGAAGAAGGAAGG
CGATAAAGTTGCCCCGGGAGACGTGATCGCCGAGATCGAGACCGACAAGGCAACCATGGAAGTGGAAGCCGTTGACGAAG
GCACGCTCGGCAAGATCCTCGTTGAAGAGGGCGCGCAGGGCGTGCGAGTCAATACGCCAATTGCCATACTGCTCGCCGAT
GGCGAGAGCGCCGACGATCTCGGCAAGGATGCGGTCACGCCGCCCCCAGCCCGCGCCCAGGCCCCGGCCGAAGTTCATGC
CGACCAGCAGGCGGAAGGCTTTCCCAGGCCCGCCGCCACGCCGAAGGCAGCCACGCAGCCCATGCCCTCCGATCCGGATA
TCCCGGAGGGTACGGAGATGGTCGAAATGACCGTGCGCGAGGCGCTGCGCGATGCCATGGCCGAGGAAATGCGGCGCGAT
GCTGATGTCTTGATCATGGGTGAGGAAGTTGCCGAATACCAGGGCGCCTACAAGGTGACCCAGGGCCTGTTGCAGGAATT
CGGCGCCAAGCGCGTGATCGACACGCCCATTACGGAGCATGGTTTCGCTGGAGTTGGCATCGGCGCGGCTTTTGCCGGGT
TGAAGCCCATTGTCGAGTTCATGACTTTCAATTTCGCCATGCAGGCGATGGACCAGATCGTCAACTCGGCTGCCAAGACG
CTCTATATGGCGGGCGGGCAGATGGGAGCGCCGATCGTCTTCCGCGGACCGAATGGCGCGGCGGCGCGCGTGGCCGCCCA
GCACAGCCAGGATTATGCCGCCTGGTACAGTCACATTCCCGGCCTGAAGGTCGTGATGCCCTATACCGCGGCAGATGCAA
AGGGCCTGCTGAAGGCTGCGATTCGCGATCCAAATCCGGTGATCTTCCTCGAAAACGAGATCCTCTACGGCCAGAGTTTC
CAGGTACCGAAGCTCGATGATTTCGTGCTGCCTATTGGCAAAGCGCGGATTCATAAGCAGGGCCGGGATGTAACGATCGT
CTCTTTCGGCATCGGCATGACCTACGCCATCAAAGCCGAGGAGGAACTGCGGGGAATGGGCATCGATGTCGAGATCATCG
ACCTGAGGACCATCCGGCCCATGGATCTCGATACCGTCGTTGAATCGGTGAAGAAAACAAACAGGCTCGTGACCATCGAA
GAAGGTTTCCCGCAATCCTCCGTCGGCGACCACATCGCTTCCAAGGTGATGCAGCGCGCTTTCGATTATCTGGATGCGCC
GATCATCACCATAGCAGGCAAGGATGTGCCGATGCCCTATGCGGCCAATCTGGAGAAGCTCGCTTTGCCGAGCGTGGTGG
AAGTGGTCGAGGCGGTGAAAGCCGTCACCTACCGGGCCTGA

Upstream 100 bases:

>100_bases
CGACATCGTCGCCGATGCGGCCGATTTCGCGCAATCCGATCCCGAGCCGGATCCGTCCGAGCTCTATACCGATATCCTGA
TCTAAGGGGGGAGTGTACCT

Downstream 100 bases:

>100_bases
GGAGGAGTAAGCCATGCCGATCCAGATCACCATGCCGGCCCTCTCGCCCACCATGGAAGAGGGCAATCTTGCCAAGTGGC
TGGTTAAGGAGGGGGACAGC

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MPTQILMPALSPTMEEGNLSKWVKKEGDKVAPGDVIAEIETDKATMEVEAVDEGTLGKILVEEGAQGVRVNTPIAILLAD
GESADDLGKDAVTPPPARAQAPAEVHADQQAEGFPRPAATPKAATQPMPSDPDIPEGTEMVEMTVREALRDAMAEEMRRD
ADVLIMGEEVAEYQGAYKVTQGLLQEFGAKRVIDTPITEHGFAGVGIGAAFAGLKPIVEFMTFNFAMQAMDQIVNSAAKT
LYMAGGQMGAPIVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQSF
QVPKLDDFVLPIGKARIHKQGRDVTIVSFGIGMTYAIKAEEELRGMGIDVEIIDLRTIRPMDLDTVVESVKKTNRLVTIE
EGFPQSSVGDHIASKVMQRAFDYLDAPIITIAGKDVPMPYAANLEKLALPSVVEVVEAVKAVTYRA

Sequences:

>Translated_466_residues
MPTQILMPALSPTMEEGNLSKWVKKEGDKVAPGDVIAEIETDKATMEVEAVDEGTLGKILVEEGAQGVRVNTPIAILLAD
GESADDLGKDAVTPPPARAQAPAEVHADQQAEGFPRPAATPKAATQPMPSDPDIPEGTEMVEMTVREALRDAMAEEMRRD
ADVLIMGEEVAEYQGAYKVTQGLLQEFGAKRVIDTPITEHGFAGVGIGAAFAGLKPIVEFMTFNFAMQAMDQIVNSAAKT
LYMAGGQMGAPIVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQSF
QVPKLDDFVLPIGKARIHKQGRDVTIVSFGIGMTYAIKAEEELRGMGIDVEIIDLRTIRPMDLDTVVESVKKTNRLVTIE
EGFPQSSVGDHIASKVMQRAFDYLDAPIITIAGKDVPMPYAANLEKLALPSVVEVVEAVKAVTYRA
>Mature_465_residues
PTQILMPALSPTMEEGNLSKWVKKEGDKVAPGDVIAEIETDKATMEVEAVDEGTLGKILVEEGAQGVRVNTPIAILLADG
ESADDLGKDAVTPPPARAQAPAEVHADQQAEGFPRPAATPKAATQPMPSDPDIPEGTEMVEMTVREALRDAMAEEMRRDA
DVLIMGEEVAEYQGAYKVTQGLLQEFGAKRVIDTPITEHGFAGVGIGAAFAGLKPIVEFMTFNFAMQAMDQIVNSAAKTL
YMAGGQMGAPIVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQSFQ
VPKLDDFVLPIGKARIHKQGRDVTIVSFGIGMTYAIKAEEELRGMGIDVEIIDLRTIRPMDLDTVVESVKKTNRLVTIEE
GFPQSSVGDHIASKVMQRAFDYLDAPIITIAGKDVPMPYAANLEKLALPSVVEVVEAVKAVTYRA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=55.2469135802469, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=51.5432098765432, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI4557353, Length=362, Percent_Identity=34.8066298342541, Blast_Score=209, Evalue=6e-54,
Organism=Homo sapiens, GI34101272, Length=362, Percent_Identity=34.8066298342541, Blast_Score=209, Evalue=6e-54,
Organism=Homo sapiens, GI203098753, Length=97, Percent_Identity=50.5154639175258, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI203098816, Length=97, Percent_Identity=50.5154639175258, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI31711992, Length=75, Percent_Identity=53.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI260898739, Length=59, Percent_Identity=61.0169491525424, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI225637461, Length=245, Percent_Identity=27.7551020408163, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI225637459, Length=245, Percent_Identity=27.7551020408163, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI225637463, Length=245, Percent_Identity=27.7551020408163, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=322, Percent_Identity=60.8695652173913, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17506935, Length=342, Percent_Identity=39.1812865497076, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17560088, Length=130, Percent_Identity=41.5384615384615, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319698, Length=325, Percent_Identity=57.5384615384615, Blast_Score=395, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324258, Length=144, Percent_Identity=45.1388888888889, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321632, Length=121, Percent_Identity=40.495867768595, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21358145, Length=321, Percent_Identity=57.0093457943925, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24650940, Length=321, Percent_Identity=57.0093457943925, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=33.7461300309598, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=33.7461300309598, Blast_Score=195, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=62.6373626373626, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=62.6373626373626, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129315, Length=74, Percent_Identity=52.7027027027027, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582497, Length=67, Percent_Identity=49.2537313432836, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45551847, Length=268, Percent_Identity=25.3731343283582, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550715, Length=268, Percent_Identity=25.3731343283582, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24645119, Length=268, Percent_Identity=25.3731343283582, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 50126; Mature: 49995

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQILMPALSPTMEEGNLSKWVKKEGDKVAPGDVIAEIETDKATMEVEAVDEGTLGKIL
CCCCEECCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHH
VEEGAQGVRVNTPIAILLADGESADDLGKDAVTPPPARAQAPAEVHADQQAEGFPRPAAT
HHCCCCCEEECCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
PKAATQPMPSDPDIPEGTEMVEMTVREALRDAMAEEMRRDADVLIMGEEVAEYQGAYKVT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHH
QGLLQEFGAKRVIDTPITEHGFAGVGIGAAFAGLKPIVEFMTFNFAMQAMDQIVNSAAKT
HHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYMAGGQMGAPIVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVMPYTAADAKGLLKAA
EEEECCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
IRDPNPVIFLENEILYGQSFQVPKLDDFVLPIGKARIHKQGRDVTIVSFGIGMTYAIKAE
HCCCCCEEEEECEEEECCCCCCCCCCCHHCCCCHHHHHCCCCCEEEEEECCCEEEEEECH
EELRGMGIDVEIIDLRTIRPMDLDTVVESVKKTNRLVTIEEGFPQSSVGDHIASKVMQRA
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
FDYLDAPIITIAGKDVPMPYAANLEKLALPSVVEVVEAVKAVTYRA
HHHHCCCEEEEECCCCCCCHHCCHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTQILMPALSPTMEEGNLSKWVKKEGDKVAPGDVIAEIETDKATMEVEAVDEGTLGKIL
CCCEECCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHH
VEEGAQGVRVNTPIAILLADGESADDLGKDAVTPPPARAQAPAEVHADQQAEGFPRPAAT
HHCCCCCEEECCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
PKAATQPMPSDPDIPEGTEMVEMTVREALRDAMAEEMRRDADVLIMGEEVAEYQGAYKVT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHH
QGLLQEFGAKRVIDTPITEHGFAGVGIGAAFAGLKPIVEFMTFNFAMQAMDQIVNSAAKT
HHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYMAGGQMGAPIVFRGPNGAAARVAAQHSQDYAAWYSHIPGLKVVMPYTAADAKGLLKAA
EEEECCCCCCCEEEECCCCCHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
IRDPNPVIFLENEILYGQSFQVPKLDDFVLPIGKARIHKQGRDVTIVSFGIGMTYAIKAE
HCCCCCEEEEECEEEECCCCCCCCCCCHHCCCCHHHHHCCCCCEEEEEECCCEEEEEECH
EELRGMGIDVEIIDLRTIRPMDLDTVVESVKKTNRLVTIEEGFPQSSVGDHIASKVMQRA
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
FDYLDAPIITIAGKDVPMPYAANLEKLALPSVVEVVEAVKAVTYRA
HHHHCCCEEEEECCCCCCCHHCCHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]