Click here to switch to the map view.

The map label for this gene is ntrX [H]

Identifier: 110633968

GI number: 110633968

Start: 1739828

End: 1741189

Strand: Reverse

Name: ntrX [H]

Synonym: Meso_1616

Alternate gene names: 110633968

Gene position: 1741189-1739828 (Counterclockwise)

Preceding gene: 110633969

Following gene: 110633967

Centisome position: 39.46

GC content: 59.18

Gene sequence:

>1362_bases
ATGGCGTCTGACATACTAGTCGTCGACGATGAGGAAGACATTCGCGAATTGGTCGCCGGAATCCTCAGCGACGAAGGACA
TGAAACCCGAACGGCGGGGAATAGCGATGCGGCACTGGCAGCGATTGCAGAGCGTGTGCCGCGGCTGGTTCTGCTTGATA
TCTGGCTTCAGGGCTCAACCCTCGACGGGTTGGCGCTCCTCGATAAGATCAAGGAGATTCATCCCGATTTGCCGGTGGTG
ATGATTTCCGGGCATGGCACCATCGAGACCGCCGTCTCTGCGATCAAGCGCGGCGCTTATGATTTCATTGAAAAGCCGTT
CAAGGCCGACAGGCTGATCCTCATCTGCGAGCGCGCGCTCGAGACTTCCAAACTTCGCCGCGAGGTGCTGGACCTCAAGA
AACGCAGCGGCGAGAGCTTTGATCTTGTCGGAAATTCGCCAGCGATCAATCATCTGCGCCAGACGATCGAGCGTATTGCG
CCCAGCAACAGCCGCGTCATGCTGATCGGTCCCTCAGGCTCGGGAAAGGAGCTGGCGGCGCGGGCGATCCACGCCCTGTC
GAACCGGGCCGGCGCTCCCTTCGTGACCTTGAACGCCGCCTCCATCACGCCGGAACGCATGGAGATCGAACTATTCGGAA
CGGAATCGAATGGCGGGGAACGTAAGGTTGGCGCGCTTGAAGAAGCTCATCGAGGTACGCTCTATCTCGACGAGGTTGCA
GACATGCCGCGCGAGACGCAGGGAAAAATCCTGCGCGTGCTCATTGACCAGCAATTTGAGCGGGTAGGGGGTACGAAGCG
GGTCAAGGTCGATGTACGCATCATCTCCTCGACGGCCCGCAACCTGGAGCGCATGATCGAGGAAGGGCGGTTCCGCGAGG
ATCTCTACCATCGCCTGGCCGTGGTGCCCGTCGCGGTTCCCGCGCTTTCGGAACGGCGCGAGGACATTCCGTTGCTTGTC
GATCATTTCATGCAGCAGATTACAAGCCAGGTGGGAATCAAGTCGCGCCGGCTCGGCGAGGATGCCATGGCGGTTCTCCA
GGCGCATGACTGGCCAGGCAATATCCGTCAGCTGCGAAACAATGTCGAACGGCTGATGATTCTGACGCGTGGCGACGACG
GTGATGACCCGATCACAGCCGACCTCCTGCCTTCGGAAATTGGCGACGTGATGCCGCGTGTTCCCACCCAATCGGACCAG
CATATCATGGCTCTGCCCTTGCGTGAGGCCCGTGAGCTGTTCGAGAAGGAATATCTTCTTGCGCAGATCAATCGATTCGG
CGGCAATATTTCTCGTACTGCCGAATTCATAGGGATGGAGCGTTCGGCGCTGCACAGGAAACTGAAGTCGCTGGGCGTCT
GA

Upstream 100 bases:

>100_bases
GTGGGAGCAATGATTCGGATGGTCTTGCCGGTCACGCAAGCGGATGCGGGGAATGGGGCAGGGACGGATAATTCGGTAGA
CAGGACGGAAAAGGTCGACA

Downstream 100 bases:

>100_bases
CGGCCCTCGGAGAATGGAATGAAAGTCATCATTTGCGGCGCGGGCCAAGTGGGCTATGGGATTGCCGAGCGCCTTGCGGC
CGAGCAGAACGATGTCTCCA

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MASDILVVDDEEDIRELVAGILSDEGHETRTAGNSDAALAAIAERVPRLVLLDIWLQGSTLDGLALLDKIKEIHPDLPVV
MISGHGTIETAVSAIKRGAYDFIEKPFKADRLILICERALETSKLRREVLDLKKRSGESFDLVGNSPAINHLRQTIERIA
PSNSRVMLIGPSGSGKELAARAIHALSNRAGAPFVTLNAASITPERMEIELFGTESNGGERKVGALEEAHRGTLYLDEVA
DMPRETQGKILRVLIDQQFERVGGTKRVKVDVRIISSTARNLERMIEEGRFREDLYHRLAVVPVAVPALSERREDIPLLV
DHFMQQITSQVGIKSRRLGEDAMAVLQAHDWPGNIRQLRNNVERLMILTRGDDGDDPITADLLPSEIGDVMPRVPTQSDQ
HIMALPLREARELFEKEYLLAQINRFGGNISRTAEFIGMERSALHRKLKSLGV

Sequences:

>Translated_453_residues
MASDILVVDDEEDIRELVAGILSDEGHETRTAGNSDAALAAIAERVPRLVLLDIWLQGSTLDGLALLDKIKEIHPDLPVV
MISGHGTIETAVSAIKRGAYDFIEKPFKADRLILICERALETSKLRREVLDLKKRSGESFDLVGNSPAINHLRQTIERIA
PSNSRVMLIGPSGSGKELAARAIHALSNRAGAPFVTLNAASITPERMEIELFGTESNGGERKVGALEEAHRGTLYLDEVA
DMPRETQGKILRVLIDQQFERVGGTKRVKVDVRIISSTARNLERMIEEGRFREDLYHRLAVVPVAVPALSERREDIPLLV
DHFMQQITSQVGIKSRRLGEDAMAVLQAHDWPGNIRQLRNNVERLMILTRGDDGDDPITADLLPSEIGDVMPRVPTQSDQ
HIMALPLREARELFEKEYLLAQINRFGGNISRTAEFIGMERSALHRKLKSLGV
>Mature_452_residues
ASDILVVDDEEDIRELVAGILSDEGHETRTAGNSDAALAAIAERVPRLVLLDIWLQGSTLDGLALLDKIKEIHPDLPVVM
ISGHGTIETAVSAIKRGAYDFIEKPFKADRLILICERALETSKLRREVLDLKKRSGESFDLVGNSPAINHLRQTIERIAP
SNSRVMLIGPSGSGKELAARAIHALSNRAGAPFVTLNAASITPERMEIELFGTESNGGERKVGALEEAHRGTLYLDEVAD
MPRETQGKILRVLIDQQFERVGGTKRVKVDVRIISSTARNLERMIEEGRFREDLYHRLAVVPVAVPALSERREDIPLLVD
HFMQQITSQVGIKSRRLGEDAMAVLQAHDWPGNIRQLRNNVERLMILTRGDDGDDPITADLLPSEIGDVMPRVPTQSDQH
IMALPLREARELFEKEYLLAQINRFGGNISRTAEFIGMERSALHRKLKSLGV

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=38.034188034188, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1788550, Length=462, Percent_Identity=37.2294372294372, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=39.1111111111111, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1788905, Length=449, Percent_Identity=34.075723830735, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1789087, Length=361, Percent_Identity=34.3490304709141, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789233, Length=233, Percent_Identity=43.7768240343348, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87082117, Length=320, Percent_Identity=36.25, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786524, Length=327, Percent_Identity=36.3914373088685, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=34.4936708860759, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787583, Length=336, Percent_Identity=32.4404761904762, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=34.472049689441, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87081858, Length=295, Percent_Identity=28.135593220339, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789828, Length=276, Percent_Identity=30.4347826086957, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=37.3913043478261, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50285; Mature: 50154

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDILVVDDEEDIRELVAGILSDEGHETRTAGNSDAALAAIAERVPRLVLLDIWLQGST
CCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCC
LDGLALLDKIKEIHPDLPVVMISGHGTIETAVSAIKRGAYDFIEKPFKADRLILICERAL
CHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHH
ETSKLRREVLDLKKRSGESFDLVGNSPAINHLRQTIERIAPSNSRVMLIGPSGSGKELAA
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RAIHALSNRAGAPFVTLNAASITPERMEIELFGTESNGGERKVGALEEAHRGTLYLDEVA
HHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCEEEHHHH
DMPRETQGKILRVLIDQQFERVGGTKRVKVDVRIISSTARNLERMIEEGRFREDLYHRLA
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHH
VVPVAVPALSERREDIPLLVDHFMQQITSQVGIKSRRLGEDAMAVLQAHDWPGNIRQLRN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCHHHHHHHH
NVERLMILTRGDDGDDPITADLLPSEIGDVMPRVPTQSDQHIMALPLREARELFEKEYLL
HHHHEEEEECCCCCCCCEEHHCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHH
AQINRFGGNISRTAEFIGMERSALHRKLKSLGV
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASDILVVDDEEDIRELVAGILSDEGHETRTAGNSDAALAAIAERVPRLVLLDIWLQGST
CCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCC
LDGLALLDKIKEIHPDLPVVMISGHGTIETAVSAIKRGAYDFIEKPFKADRLILICERAL
CHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHH
ETSKLRREVLDLKKRSGESFDLVGNSPAINHLRQTIERIAPSNSRVMLIGPSGSGKELAA
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RAIHALSNRAGAPFVTLNAASITPERMEIELFGTESNGGERKVGALEEAHRGTLYLDEVA
HHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCEEEHHHH
DMPRETQGKILRVLIDQQFERVGGTKRVKVDVRIISSTARNLERMIEEGRFREDLYHRLA
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHH
VVPVAVPALSERREDIPLLVDHFMQQITSQVGIKSRRLGEDAMAVLQAHDWPGNIRQLRN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHCCCCHHHHHHHH
NVERLMILTRGDDGDDPITADLLPSEIGDVMPRVPTQSDQHIMALPLREARELFEKEYLL
HHHHEEEEECCCCCCCCEEHHCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHH
AQINRFGGNISRTAEFIGMERSALHRKLKSLGV
HHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]