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The map label for this gene is holB [H]

Identifier: 110633934

GI number: 110633934

Start: 1702603

End: 1703667

Strand: Direct

Name: holB [H]

Synonym: Meso_1582

Alternate gene names: 110633934

Gene position: 1702603-1703667 (Clockwise)

Preceding gene: 110633933

Following gene: 110633935

Centisome position: 38.59

GC content: 58.69

Gene sequence:

>1065_bases
GTGTTTGAACGCCGCGCGCCTGAACAGCACGATTCTCTGCCGGATATCCCGGAACCGCCGGAAAACCCGCTCCTTGTCGG
CCACGAAAACGCACGCTCAATGCTTGCCAGCGCCTATCGGGCCGGCAAGCTCCACCATGCACTCCTTTTTGCTGGAAGAC
CCGGCATTGGAAAGGCAACGCTTGCCTTCCACCTGGCGCATCATCTCTTTACCAATCCGGATCACCGCCTGGGACCGTTG
GATTTTTCCGACATCGATACGAACTCGCAGGATTTCCGGCTTGCGGCGCAGGGCGCCCATCCCGGCGTCCTTCACTTGAC
TCGGCCTTTTGTCGAGCGAGACAAGAAATTCAAGACTGTGATTACCGTCGACGAAATACGCCGGGTGGCTCGATTTCTCT
CCCTGACGCCCCCGGACGGCGGCTATCGCGTGGTGATTGTCGACCCCGCGGACGACATGAACGCCAATGCTGCAAATGCA
TTGCTGAAAAACCTGGAGGAGCCACCTCCGCGCACGATCTTCATTCTGATCACGCATTCTTTCGGCCGGCTCCTGCCCAC
GATCCGCTCACGCTGCCAGGTCTATAAGCTCGATCCGCTTTCGCCCGGGCTTCTGGAGAAACTTCTACAAAGCTTGGGCG
CGAGCCTGCCCGAAACTGAAGAAGAAAGGCGTCTCCTCATCCAACGCGCTCAGGGAAGTGCCCGCGATGCGATCCTGCTC
ACCGAATTTGGTGGATTGGAAATAGAGCAGGCGATCGAGAACCTTCTCGAGCAGCGCCGTTTCGATGTACCCGCGGCTTA
CAAGCTTGCGGAGGCTGCCACAGGGCGGGAACGCCAGGTGCAGTTCGACCTTTTTAACCGGGCTGTGCTCGACCGGGTAG
CCAACGCGGCGTCACACAGTGCTTCAGCGGGCGACATCGCGCAGGCCGAGCGCTTTGCAAATTTCTGGGAAGAATTGGCG
GAAACGATCCGCCAAGTCGAGATTTTCAACCTCGACCGGAAACAGCACGTTGTGAGCTCTCTCAACCGCCTGCACAATGC
CATGCATGTTCTGATGGCGGGCTAG

Upstream 100 bases:

>100_bases
GCCTCAAGATCAGCCGACGCGGTTTCGGAGAATGTGACCGAGATTGTCTTGCGAGTCATATTTGATAGAGCAGGGCTCTC
GGAACCAACGGAGGCGGGCA

Downstream 100 bases:

>100_bases
GCACCCAAAGCATGGCAATCCCCGCCGCGATACGCTATCTGGGCGCAAGCAATATCGACCTAGAGCATTCTCCTGATGAC
CCGCGAGACTTTCTACATCA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MFERRAPEQHDSLPDIPEPPENPLLVGHENARSMLASAYRAGKLHHALLFAGRPGIGKATLAFHLAHHLFTNPDHRLGPL
DFSDIDTNSQDFRLAAQGAHPGVLHLTRPFVERDKKFKTVITVDEIRRVARFLSLTPPDGGYRVVIVDPADDMNANAANA
LLKNLEEPPPRTIFILITHSFGRLLPTIRSRCQVYKLDPLSPGLLEKLLQSLGASLPETEEERRLLIQRAQGSARDAILL
TEFGGLEIEQAIENLLEQRRFDVPAAYKLAEAATGRERQVQFDLFNRAVLDRVANAASHSASAGDIAQAERFANFWEELA
ETIRQVEIFNLDRKQHVVSSLNRLHNAMHVLMAG

Sequences:

>Translated_354_residues
MFERRAPEQHDSLPDIPEPPENPLLVGHENARSMLASAYRAGKLHHALLFAGRPGIGKATLAFHLAHHLFTNPDHRLGPL
DFSDIDTNSQDFRLAAQGAHPGVLHLTRPFVERDKKFKTVITVDEIRRVARFLSLTPPDGGYRVVIVDPADDMNANAANA
LLKNLEEPPPRTIFILITHSFGRLLPTIRSRCQVYKLDPLSPGLLEKLLQSLGASLPETEEERRLLIQRAQGSARDAILL
TEFGGLEIEQAIENLLEQRRFDVPAAYKLAEAATGRERQVQFDLFNRAVLDRVANAASHSASAGDIAQAERFANFWEELA
ETIRQVEIFNLDRKQHVVSSLNRLHNAMHVLMAG
>Mature_354_residues
MFERRAPEQHDSLPDIPEPPENPLLVGHENARSMLASAYRAGKLHHALLFAGRPGIGKATLAFHLAHHLFTNPDHRLGPL
DFSDIDTNSQDFRLAAQGAHPGVLHLTRPFVERDKKFKTVITVDEIRRVARFLSLTPPDGGYRVVIVDPADDMNANAANA
LLKNLEEPPPRTIFILITHSFGRLLPTIRSRCQVYKLDPLSPGLLEKLLQSLGASLPETEEERRLLIQRAQGSARDAILL
TEFGGLEIEQAIENLLEQRRFDVPAAYKLAEAATGRERQVQFDLFNRAVLDRVANAASHSASAGDIAQAERFANFWEELA
ETIRQVEIFNLDRKQHVVSSLNRLHNAMHVLMAG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=223, Percent_Identity=31.390134529148, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787341, Length=165, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39555; Mature: 39555

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERRAPEQHDSLPDIPEPPENPLLVGHENARSMLASAYRAGKLHHALLFAGRPGIGKAT
CCCCCCCHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCHHHEEEEECCCCCCHHH
LAFHLAHHLFTNPDHRLGPLDFSDIDTNSQDFRLAAQGAHPGVLHLTRPFVERDKKFKTV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHCCHHHHE
ITVDEIRRVARFLSLTPPDGGYRVVIVDPADDMNANAANALLKNLEEPPPRTIFILITHS
EEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECH
FGRLLPTIRSRCQVYKLDPLSPGLLEKLLQSLGASLPETEEERRLLIQRAQGSARDAILL
HHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEE
TEFGGLEIEQAIENLLEQRRFDVPAAYKLAEAATGRERQVQFDLFNRAVLDRVANAASHS
ECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCC
ASAGDIAQAERFANFWEELAETIRQVEIFNLDRKQHVVSSLNRLHNAMHVLMAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFERRAPEQHDSLPDIPEPPENPLLVGHENARSMLASAYRAGKLHHALLFAGRPGIGKAT
CCCCCCCHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCHHHEEEEECCCCCCHHH
LAFHLAHHLFTNPDHRLGPLDFSDIDTNSQDFRLAAQGAHPGVLHLTRPFVERDKKFKTV
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHCCHHHHE
ITVDEIRRVARFLSLTPPDGGYRVVIVDPADDMNANAANALLKNLEEPPPRTIFILITHS
EEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECH
FGRLLPTIRSRCQVYKLDPLSPGLLEKLLQSLGASLPETEEERRLLIQRAQGSARDAILL
HHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEE
TEFGGLEIEQAIENLLEQRRFDVPAAYKLAEAATGRERQVQFDLFNRAVLDRVANAASHS
ECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCC
ASAGDIAQAERFANFWEELAETIRQVEIFNLDRKQHVVSSLNRLHNAMHVLMAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]