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The map label for this gene is metG [H]

Identifier: 110633935

GI number: 110633935

Start: 1703743

End: 1705296

Strand: Direct

Name: metG [H]

Synonym: Meso_1583

Alternate gene names: 110633935

Gene position: 1703743-1705296 (Clockwise)

Preceding gene: 110633934

Following gene: 110633936

Centisome position: 38.61

GC content: 61.39

Gene sequence:

>1554_bases
ATGACCCGCGAGACTTTCTACATCACCACGGCCATTTCCTATCCGAACGGGCGCCCCCACATTGGCCACGCCTACGAGCT
CATCGCGACGGACGCGCTGGCTCGTTTCCAGCGCTGCGACGGCAAGGACGTGATGTTTTTGACCGGCACCGATGAGCACG
GCATCAAGATGCTGCAGACCGCGCGCAAGGAAGGGATTACCGCCGGCGAGCTGGCAGACCGCAATGCGCCGGAATTCCAG
CGCATGGCCAAGCTGCTGAATGCCTCGAACGACGATTTTATCCGTACGACCGAAGAGCGCCATCGCACCGCCTCTCAGGC
AATCTGGAAGGCGATGGCCGATAGGGGCGACATTTATCTGGGCGGCTATTCCGGATGGTACTCGGTGCGCGACGAGGCCT
ATTACGGCGAGGACGAGACGCGGGTGGGCGAGGACGGCGTCCGCTACGGGCCACAGGGCACGCCGGTGGAATGGGTGGAA
GAGGAAAGCTATTTCTTCCGCCTCTCCGCCTATCAGGAACGTCTCTTGAAGCTCTACGAGGAAAATCCGGATTTCATCGG
GCCGGCCGAGCGCCGGAATGAAATCGTCAGCTTTGTGCGCTCGGGCTTGAGGGACCTTTCCATCTCACGCACAACTTTCG
ACTGGGGTGTGCCGGTACCGGGCGACGAGAAGCACGTGATGTATGTGTGGGTGGATGCGCTGACCAATTACATCACGGCC
CTCGGCTATCCCGATCAGGATGCGGAGCGCTGGCGCTACTGGCCCGCCGACGTTCATATCATCGGCAAGGATATCATCCG
CTTTCATGCCATCTACTGGCCCGCATTCCTGATGTCGGCCGGTATCGCGCTGCCAAAGCGCGTCTTCGGTCACGGCTTCC
TGTTCAACCGCGGCGAGAAGATGTCGAAGTCGGTTGGCAATGTCATCGACCCCTTCACAATGATCGAACATTACGGCCTC
GACCAGGTGCGCTATTTCTTCCTGCGGGAAGTTCCCTTTGGTCAGGACGGAAGCTACAGCCATGAGGCGATCGTCAACCG
CACCAACGCCGATCTTGCAAACGACCTCGGCAACCTGGCGCAGCGTTCGCTCTCCATGATCGCGAAGAACTGCGACGGCA
AAGTGCCTGCGAGAGGCGAACTCTCGGCGGCGGATCGAGCAATCCTCGACCAGGCCGAAGCCGCGCTTGCAACCGCGAGA
GGAGCAATGGGCCGACAGGCGATCCACGCTGCCCTTGCGGCCATCTTCGATGTGGTCGCGGAAGCAAACCGTTATTTTGC
AGCACAGGAGCCGTGGGCGCTGCGCAAGACGGACCCGGCCCGCATGGAAACGGTGCTCTACACCACCGCCGAGACATTGC
GGCGCATCGCCATTCTGTGCCAGCCCTTCATCCCCAGCTCGGCCGAGAAACTCCTCGATCTGCTTTCCGTGCCTCAGGAT
CGCCGCGGTTTTGCCGATGTAGGAGAGACGGCGATGCTCGCGCCGGGAACGCCGCTGCCGGCGCCTCAACCGGTTTTCCC
GCGTTATGTGGAGCAGGAAGGGCAGGAGGGCTGA

Upstream 100 bases:

>100_bases
CATGCATGTTCTGATGGCGGGCTAGGCACCCAAAGCATGGCAATCCCCGCCGCGATACGCTATCTGGGCGCAAGCAATAT
CGACCTAGAGCATTCTCCTG

Downstream 100 bases:

>100_bases
ATGCTGGTCGACAGCCATTGCCACCTGGATTTCCCGGATTTCGAAGCGGAGCGGGACCAGATCATCGCCCGCGCTGGCGA
TGCCGGCGTGGGCCTGATGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTRETFYITTAISYPNGRPHIGHAYELIATDALARFQRCDGKDVMFLTGTDEHGIKMLQTARKEGITAGELADRNAPEFQ
RMAKLLNASNDDFIRTTEERHRTASQAIWKAMADRGDIYLGGYSGWYSVRDEAYYGEDETRVGEDGVRYGPQGTPVEWVE
EESYFFRLSAYQERLLKLYEENPDFIGPAERRNEIVSFVRSGLRDLSISRTTFDWGVPVPGDEKHVMYVWVDALTNYITA
LGYPDQDAERWRYWPADVHIIGKDIIRFHAIYWPAFLMSAGIALPKRVFGHGFLFNRGEKMSKSVGNVIDPFTMIEHYGL
DQVRYFFLREVPFGQDGSYSHEAIVNRTNADLANDLGNLAQRSLSMIAKNCDGKVPARGELSAADRAILDQAEAALATAR
GAMGRQAIHAALAAIFDVVAEANRYFAAQEPWALRKTDPARMETVLYTTAETLRRIAILCQPFIPSSAEKLLDLLSVPQD
RRGFADVGETAMLAPGTPLPAPQPVFPRYVEQEGQEG

Sequences:

>Translated_517_residues
MTRETFYITTAISYPNGRPHIGHAYELIATDALARFQRCDGKDVMFLTGTDEHGIKMLQTARKEGITAGELADRNAPEFQ
RMAKLLNASNDDFIRTTEERHRTASQAIWKAMADRGDIYLGGYSGWYSVRDEAYYGEDETRVGEDGVRYGPQGTPVEWVE
EESYFFRLSAYQERLLKLYEENPDFIGPAERRNEIVSFVRSGLRDLSISRTTFDWGVPVPGDEKHVMYVWVDALTNYITA
LGYPDQDAERWRYWPADVHIIGKDIIRFHAIYWPAFLMSAGIALPKRVFGHGFLFNRGEKMSKSVGNVIDPFTMIEHYGL
DQVRYFFLREVPFGQDGSYSHEAIVNRTNADLANDLGNLAQRSLSMIAKNCDGKVPARGELSAADRAILDQAEAALATAR
GAMGRQAIHAALAAIFDVVAEANRYFAAQEPWALRKTDPARMETVLYTTAETLRRIAILCQPFIPSSAEKLLDLLSVPQD
RRGFADVGETAMLAPGTPLPAPQPVFPRYVEQEGQEG
>Mature_516_residues
TRETFYITTAISYPNGRPHIGHAYELIATDALARFQRCDGKDVMFLTGTDEHGIKMLQTARKEGITAGELADRNAPEFQR
MAKLLNASNDDFIRTTEERHRTASQAIWKAMADRGDIYLGGYSGWYSVRDEAYYGEDETRVGEDGVRYGPQGTPVEWVEE
ESYFFRLSAYQERLLKLYEENPDFIGPAERRNEIVSFVRSGLRDLSISRTTFDWGVPVPGDEKHVMYVWVDALTNYITAL
GYPDQDAERWRYWPADVHIIGKDIIRFHAIYWPAFLMSAGIALPKRVFGHGFLFNRGEKMSKSVGNVIDPFTMIEHYGLD
QVRYFFLREVPFGQDGSYSHEAIVNRTNADLANDLGNLAQRSLSMIAKNCDGKVPARGELSAADRAILDQAEAALATARG
AMGRQAIHAALAAIFDVVAEANRYFAAQEPWALRKTDPARMETVLYTTAETLRRIAILCQPFIPSSAEKLLDLLSVPQDR
RGFADVGETAMLAPGTPLPAPQPVFPRYVEQEGQEG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=510, Percent_Identity=38.8235294117647, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI14043022, Length=520, Percent_Identity=26.1538461538462, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1788432, Length=500, Percent_Identity=26.6, Blast_Score=174, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI212645096, Length=498, Percent_Identity=34.3373493975904, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI71992781, Length=387, Percent_Identity=33.8501291989664, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI17540794, Length=527, Percent_Identity=24.2884250474383, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321610, Length=531, Percent_Identity=32.9566854990584, Blast_Score=289, Evalue=8e-79,
Organism=Saccharomyces cerevisiae, GI6321704, Length=510, Percent_Identity=21.7647058823529, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21358483, Length=511, Percent_Identity=36.986301369863, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24655311, Length=545, Percent_Identity=24.7706422018349, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58138; Mature: 58007

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRETFYITTAISYPNGRPHIGHAYELIATDALARFQRCDGKDVMFLTGTDEHGIKMLQT
CCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
ARKEGITAGELADRNAPEFQRMAKLLNASNDDFIRTTEERHRTASQAIWKAMADRGDIYL
HHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCEEE
GGYSGWYSVRDEAYYGEDETRVGEDGVRYGPQGTPVEWVEEESYFFRLSAYQERLLKLYE
CCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHH
ENPDFIGPAERRNEIVSFVRSGLRDLSISRTTFDWGVPVPGDEKHVMYVWVDALTNYITA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHH
LGYPDQDAERWRYWPADVHIIGKDIIRFHAIYWPAFLMSAGIALPKRVFGHGFLFNRGEK
CCCCCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCCHH
MSKSVGNVIDPFTMIEHYGLDQVRYFFLREVPFGQDGSYSHEAIVNRTNADLANDLGNLA
HHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
QRSLSMIAKNCDGKVPARGELSAADRAILDQAEAALATARGAMGRQAIHAALAAIFDVVA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EANRYFAAQEPWALRKTDPARMETVLYTTAETLRRIAILCQPFIPSSAEKLLDLLSVPQD
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
RRGFADVGETAMLAPGTPLPAPQPVFPRYVEQEGQEG
CCCCHHCCCCEEECCCCCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
TRETFYITTAISYPNGRPHIGHAYELIATDALARFQRCDGKDVMFLTGTDEHGIKMLQT
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
ARKEGITAGELADRNAPEFQRMAKLLNASNDDFIRTTEERHRTASQAIWKAMADRGDIYL
HHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHCCCCEEE
GGYSGWYSVRDEAYYGEDETRVGEDGVRYGPQGTPVEWVEEESYFFRLSAYQERLLKLYE
CCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHH
ENPDFIGPAERRNEIVSFVRSGLRDLSISRTTFDWGVPVPGDEKHVMYVWVDALTNYITA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHH
LGYPDQDAERWRYWPADVHIIGKDIIRFHAIYWPAFLMSAGIALPKRVFGHGFLFNRGEK
CCCCCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCCHH
MSKSVGNVIDPFTMIEHYGLDQVRYFFLREVPFGQDGSYSHEAIVNRTNADLANDLGNLA
HHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
QRSLSMIAKNCDGKVPARGELSAADRAILDQAEAALATARGAMGRQAIHAALAAIFDVVA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EANRYFAAQEPWALRKTDPARMETVLYTTAETLRRIAILCQPFIPSSAEKLLDLLSVPQD
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
RRGFADVGETAMLAPGTPLPAPQPVFPRYVEQEGQEG
CCCCHHCCCCEEECCCCCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]