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The map label for this gene is dme [H]

Identifier: 110633889

GI number: 110633889

Start: 1652931

End: 1655243

Strand: Reverse

Name: dme [H]

Synonym: Meso_1536

Alternate gene names: 110633889

Gene position: 1655243-1652931 (Counterclockwise)

Preceding gene: 110633890

Following gene: 110633886

Centisome position: 37.51

GC content: 61.18

Gene sequence:

>2313_bases
ATGGTTGTAAAGAAAACACGTTCGGACTTGGATGAGGCTGCTCTTTTTTTTCATCGTTATCCCGTGCCGGGAAAGCTGGA
AATTCAGCCGACGAAGCCCCTTGGCAACCAGCGTGACCTCGCGCTCGCCTACTCTCCGGGCGTGGCTGCGCCGTGCCTTG
CGATTCACGAAGATCCGGCAACCGCCGCCGATTATACCTCGCGGGCAAATCTGGTGGGTGTGGTATCCAATGGCACGGCC
GTGCTTGGCCTTGGTGATATCGGACCGCTCGCCTCAAAGCCCGTGATGGAGGGCAAGGCAGTCCTCTTCAAAAAGTTCGC
CGGCATCAATGTATTCGATATCGAGATCGATGCGCCGGATGTGGACCGCATGGTGGAGACCATCTCGGCACTGGAGCCCA
CCTTCGGCGGGATCAATCTGGAGGACATCAAGGCGCCCGAATGCTTTGAGGTGGAAGAGCGCCTCAAGGAGCGCATGAAA
ATCCCGGTTTTCCATGATGACCAGCACGGCACCGCGATCATCGTTGCGGCTGCGGTGCTCAACGGGCTGGAACTTGCCGG
AAAGGATATCGCCTCCGTCAAAATCGTCACTTCCGGCGCGGGCGCAGCGGCGCTAGCTTGCCTCAACCTGCTCGTCTCGC
TCGGCGTGAAGACGGAAAATATCTGGATCACGGACCGGCATGGTGTCGCCTATCGCGGGCGCGTTGAGGAAATGGACCGG
TGGAAGGAACCCTATCTCAAGGACACTGAGGCGCGCGAGCTTGCGGATGTCATCGATGGCGCGGATGTGTTCCTCGGCCT
TTCGGCAGGCGGCGTGCTGAAGCCCGAGATGCTGAAGGGCATGGCGGAGCGGCCGCTCATCCTGGCGCTCGCCAATCCCA
ATCCGGAGATCATGCCGGACGTTGCGCGCAAGGCGCGCGCGGATGCGATGATTTGCACAGGGCGGTCGGATTTCCCGAAC
CAGGTCAACAACGTCCTTTGCTTCCCTTATATTTTCCGGGGCGCACTCGATGTCGGCGCCACGGCGATCAATGAAGAGAT
GAAGCTTGCCGCAGTGCGCGCCATCGCCGCGCTCGCCCGCGAGGAGCCTTCGGATGTGGCCGCCCGCGCCTATTCAACCG
AGACACCAGTCTTCGGGCCGGATTTTCTCATCCCCTCGCCTTTCGACCCGCGGCTGATCCTCAGGATCGCCCCGGCGGTG
GCGAAGGCGGCCATGGAGTCGGGCGTGGCGACCCGCCCGATTGAGGAAATGGATGCCTATCTGGACCGGCTGAACCGGTT
CGTCTTCCGCTCCGGGCTTGTCATGAAGCCTGTCTTCTCGACGGCTAAGCAGATCGGACGAAAGCGCGTCATCTACGCGG
ACGGCGAGGATGAGCGGGTGTTGCGTGCGGCCCAGGTGGTGCTGGAAGAGGGACTTGCGCAGCCGATCCTTATCGGCCGC
CCGCATGTCGTGGAAATGCGTCTGAAGAGATACGGCCTCAGGATCAAGCCTGGCGTCGATTTCGAGATTATCAATCCGGA
AGACGACCCGCGTTACCGAGACTATGTAGATCTCCTCATCCGGATTGCGGGTCGGCGCGGCGTGACCACGGAGGTGGCGC
GCACACTCGTGCGGACCAACGCCACCGTTATCGCTGCGCTGGCCATCATGCGCGATGAGGCGGACGCGATGATCTGCGGC
CTTGAAGGCCGTTTCGAACGGCATCTTCGATATGTAGAGGCGATCATCGGCCGGCGCGAGGGCGTACAGGACCTTTCCGC
GCTGTCGATGCTCATTACACAGCAAGGCGTGGTGTTTCTTACCGATACCTATGTGACGCTCGACCCCTCTGCGGCGGAGA
TCGCGGAAATGACGGTTCTTGCCGCGGAGGAGATCCGCCGCTTCGGCATAGACCCCAAGGCGGCGCTTCTCTCGCATTCA
GATTTCGGCTCGCGCGACTCAGCAAGCGCGATGAAAATGCGAGAGGCCGTCGGCATCTTGCACCGCATCGCGCCGGATCT
CATCGCCGATGGCGAAATGCACGGAGACGCCGCACTTTCAGAGGAGCTGCGGCAGCGCGTCTATCCGCATGCGCTGCTGA
AGGGGGAGGCAAACCTGCTTGTGTTCCCCAACCTGGATGCCGCCAACATCACGCTTACGACGCTAAAGGTCATGACGGAT
GCGCTCCATGTAGGGCCGATCCTGCTCGGCATGGCCCGGCCGGCTCATATCCTCACACCTTCCGTCACCTCGCGCGGCAT
CGTTAATATGACGGCGCTTTCCGCGGTGGAGGCTCTACAGAGCCAGAACAGACAACTGAATCTGCCAGGCTAA

Upstream 100 bases:

>100_bases
TAGGCGTGAGCGGCTTGGGGGAGCAGCACCAGCATTTTGTCGCGCTGATTTGAATTTCACCATATCCGACAATGAGGAAG
GGGCGCAGCCGAAAAGGGCC

Downstream 100 bases:

>100_bases
CACACGATTAATAACGGCCTGATACACTTCCGTTTATCATCTGCGGTCCCTGGAATGAGCCGCAGATGATCTGTCGGCGG
GGTGTGTTATGCGTCTTTCA

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MVVKKTRSDLDEAALFFHRYPVPGKLEIQPTKPLGNQRDLALAYSPGVAAPCLAIHEDPATAADYTSRANLVGVVSNGTA
VLGLGDIGPLASKPVMEGKAVLFKKFAGINVFDIEIDAPDVDRMVETISALEPTFGGINLEDIKAPECFEVEERLKERMK
IPVFHDDQHGTAIIVAAAVLNGLELAGKDIASVKIVTSGAGAAALACLNLLVSLGVKTENIWITDRHGVAYRGRVEEMDR
WKEPYLKDTEARELADVIDGADVFLGLSAGGVLKPEMLKGMAERPLILALANPNPEIMPDVARKARADAMICTGRSDFPN
QVNNVLCFPYIFRGALDVGATAINEEMKLAAVRAIAALAREEPSDVAARAYSTETPVFGPDFLIPSPFDPRLILRIAPAV
AKAAMESGVATRPIEEMDAYLDRLNRFVFRSGLVMKPVFSTAKQIGRKRVIYADGEDERVLRAAQVVLEEGLAQPILIGR
PHVVEMRLKRYGLRIKPGVDFEIINPEDDPRYRDYVDLLIRIAGRRGVTTEVARTLVRTNATVIAALAIMRDEADAMICG
LEGRFERHLRYVEAIIGRREGVQDLSALSMLITQQGVVFLTDTYVTLDPSAAEIAEMTVLAAEEIRRFGIDPKAALLSHS
DFGSRDSASAMKMREAVGILHRIAPDLIADGEMHGDAALSEELRQRVYPHALLKGEANLLVFPNLDAANITLTTLKVMTD
ALHVGPILLGMARPAHILTPSVTSRGIVNMTALSAVEALQSQNRQLNLPG

Sequences:

>Translated_770_residues
MVVKKTRSDLDEAALFFHRYPVPGKLEIQPTKPLGNQRDLALAYSPGVAAPCLAIHEDPATAADYTSRANLVGVVSNGTA
VLGLGDIGPLASKPVMEGKAVLFKKFAGINVFDIEIDAPDVDRMVETISALEPTFGGINLEDIKAPECFEVEERLKERMK
IPVFHDDQHGTAIIVAAAVLNGLELAGKDIASVKIVTSGAGAAALACLNLLVSLGVKTENIWITDRHGVAYRGRVEEMDR
WKEPYLKDTEARELADVIDGADVFLGLSAGGVLKPEMLKGMAERPLILALANPNPEIMPDVARKARADAMICTGRSDFPN
QVNNVLCFPYIFRGALDVGATAINEEMKLAAVRAIAALAREEPSDVAARAYSTETPVFGPDFLIPSPFDPRLILRIAPAV
AKAAMESGVATRPIEEMDAYLDRLNRFVFRSGLVMKPVFSTAKQIGRKRVIYADGEDERVLRAAQVVLEEGLAQPILIGR
PHVVEMRLKRYGLRIKPGVDFEIINPEDDPRYRDYVDLLIRIAGRRGVTTEVARTLVRTNATVIAALAIMRDEADAMICG
LEGRFERHLRYVEAIIGRREGVQDLSALSMLITQQGVVFLTDTYVTLDPSAAEIAEMTVLAAEEIRRFGIDPKAALLSHS
DFGSRDSASAMKMREAVGILHRIAPDLIADGEMHGDAALSEELRQRVYPHALLKGEANLLVFPNLDAANITLTTLKVMTD
ALHVGPILLGMARPAHILTPSVTSRGIVNMTALSAVEALQSQNRQLNLPG
>Mature_770_residues
MVVKKTRSDLDEAALFFHRYPVPGKLEIQPTKPLGNQRDLALAYSPGVAAPCLAIHEDPATAADYTSRANLVGVVSNGTA
VLGLGDIGPLASKPVMEGKAVLFKKFAGINVFDIEIDAPDVDRMVETISALEPTFGGINLEDIKAPECFEVEERLKERMK
IPVFHDDQHGTAIIVAAAVLNGLELAGKDIASVKIVTSGAGAAALACLNLLVSLGVKTENIWITDRHGVAYRGRVEEMDR
WKEPYLKDTEARELADVIDGADVFLGLSAGGVLKPEMLKGMAERPLILALANPNPEIMPDVARKARADAMICTGRSDFPN
QVNNVLCFPYIFRGALDVGATAINEEMKLAAVRAIAALAREEPSDVAARAYSTETPVFGPDFLIPSPFDPRLILRIAPAV
AKAAMESGVATRPIEEMDAYLDRLNRFVFRSGLVMKPVFSTAKQIGRKRVIYADGEDERVLRAAQVVLEEGLAQPILIGR
PHVVEMRLKRYGLRIKPGVDFEIINPEDDPRYRDYVDLLIRIAGRRGVTTEVARTLVRTNATVIAALAIMRDEADAMICG
LEGRFERHLRYVEAIIGRREGVQDLSALSMLITQQGVVFLTDTYVTLDPSAAEIAEMTVLAAEEIRRFGIDPKAALLSHS
DFGSRDSASAMKMREAVGILHRIAPDLIADGEMHGDAALSEELRQRVYPHALLKGEANLLVFPNLDAANITLTTLKVMTD
ALHVGPILLGMARPAHILTPSVTSRGIVNMTALSAVEALQSQNRQLNLPG

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=349, Percent_Identity=26.0744985673352, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI62420880, Length=349, Percent_Identity=26.0744985673352, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI239049447, Length=349, Percent_Identity=26.0744985673352, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4505145, Length=351, Percent_Identity=25.3561253561254, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI4505143, Length=342, Percent_Identity=25.4385964912281, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI270265879, Length=318, Percent_Identity=26.4150943396226, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1788806, Length=755, Percent_Identity=59.4701986754967, Blast_Score=917, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=352, Percent_Identity=28.125, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788800, Length=324, Percent_Identity=30.2469135802469, Blast_Score=138, Evalue=2e-33,
Organism=Escherichia coli, GI87081919, Length=461, Percent_Identity=23.2104121475054, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537199, Length=357, Percent_Identity=24.6498599439776, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=234, Percent_Identity=29.4871794871795, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281362674, Length=456, Percent_Identity=25.8771929824561, Blast_Score=90, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21356279, Length=424, Percent_Identity=26.6509433962264, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281362672, Length=424, Percent_Identity=26.6509433962264, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 83636; Mature: 83636

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVKKTRSDLDEAALFFHRYPVPGKLEIQPTKPLGNQRDLALAYSPGVAAPCLAIHEDPA
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCC
TAADYTSRANLVGVVSNGTAVLGLGDIGPLASKPVMEGKAVLFKKFAGINVFDIEIDAPD
CHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCC
VDRMVETISALEPTFGGINLEDIKAPECFEVEERLKERMKIPVFHDDQHGTAIIVAAAVL
HHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCEECCCCCCEEEHHHHHH
NGLELAGKDIASVKIVTSGAGAAALACLNLLVSLGVKTENIWITDRHGVAYRGRVEEMDR
HHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCHHHHHH
WKEPYLKDTEARELADVIDGADVFLGLSAGGVLKPEMLKGMAERPLILALANPNPEIMPD
HCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCCCCHH
VARKARADAMICTGRSDFPNQVNNVLCFPYIFRGALDVGATAINEEMKLAAVRAIAALAR
HHHHHHCCEEEECCCCCCCHHHHCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHC
EEPSDVAARAYSTETPVFGPDFLIPSPFDPRLILRIAPAVAKAAMESGVATRPIEEMDAY
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHH
LDRLNRFVFRSGLVMKPVFSTAKQIGRKRVIYADGEDERVLRAAQVVLEEGLAQPILIGR
HHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEECC
PHVVEMRLKRYGLRIKPGVDFEIINPEDDPRYRDYVDLLIRIAGRRGVTTEVARTLVRTN
CHHHHHHHHHCCCEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
ATVIAALAIMRDEADAMICGLEGRFERHLRYVEAIIGRREGVQDLSALSMLITQQGVVFL
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEE
TDTYVTLDPSAAEIAEMTVLAAEEIRRFGIDPKAALLSHSDFGSRDSASAMKMREAVGIL
EEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
HRIAPDLIADGEMHGDAALSEELRQRVYPHALLKGEANLLVFPNLDAANITLTTLKVMTD
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHEECCCCCEEEECCCCCCCEEHHHHHHHHH
ALHVGPILLGMARPAHILTPSVTSRGIVNMTALSAVEALQSQNRQLNLPG
HHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCEECCCC
>Mature Secondary Structure
MVVKKTRSDLDEAALFFHRYPVPGKLEIQPTKPLGNQRDLALAYSPGVAAPCLAIHEDPA
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCC
TAADYTSRANLVGVVSNGTAVLGLGDIGPLASKPVMEGKAVLFKKFAGINVFDIEIDAPD
CHHHHCCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCC
VDRMVETISALEPTFGGINLEDIKAPECFEVEERLKERMKIPVFHDDQHGTAIIVAAAVL
HHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCEECCCCCCEEEHHHHHH
NGLELAGKDIASVKIVTSGAGAAALACLNLLVSLGVKTENIWITDRHGVAYRGRVEEMDR
HHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCHHHHHH
WKEPYLKDTEARELADVIDGADVFLGLSAGGVLKPEMLKGMAERPLILALANPNPEIMPD
HCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCCCCHH
VARKARADAMICTGRSDFPNQVNNVLCFPYIFRGALDVGATAINEEMKLAAVRAIAALAR
HHHHHHCCEEEECCCCCCCHHHHCEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHC
EEPSDVAARAYSTETPVFGPDFLIPSPFDPRLILRIAPAVAKAAMESGVATRPIEEMDAY
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHH
LDRLNRFVFRSGLVMKPVFSTAKQIGRKRVIYADGEDERVLRAAQVVLEEGLAQPILIGR
HHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEECC
PHVVEMRLKRYGLRIKPGVDFEIINPEDDPRYRDYVDLLIRIAGRRGVTTEVARTLVRTN
CHHHHHHHHHCCCEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
ATVIAALAIMRDEADAMICGLEGRFERHLRYVEAIIGRREGVQDLSALSMLITQQGVVFL
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEE
TDTYVTLDPSAAEIAEMTVLAAEEIRRFGIDPKAALLSHSDFGSRDSASAMKMREAVGIL
EEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHH
HRIAPDLIADGEMHGDAALSEELRQRVYPHALLKGEANLLVFPNLDAANITLTTLKVMTD
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHEECCCCCEEEECCCCCCCEEHHHHHHHHH
ALHVGPILLGMARPAHILTPSVTSRGIVNMTALSAVEALQSQNRQLNLPG
HHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]