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The map label for this gene is dnaG [H]

Identifier: 110633817

GI number: 110633817

Start: 1562586

End: 1564505

Strand: Direct

Name: dnaG [H]

Synonym: Meso_1464

Alternate gene names: 110633817

Gene position: 1562586-1564505 (Clockwise)

Preceding gene: 110633816

Following gene: 110633818

Centisome position: 35.41

GC content: 64.64

Gene sequence:

>1920_bases
ATGCGCTTTCCAAACAGCTTTCTTGACGAAATCCGTGACCGCGTGCCCATCAGTCAAGTGGTGGGCTCGCGTGTCACTTT
CGACAAGCGCAAGAGCAATCCCGGTCGCGGCGATTTCTGGGCCTGCTGCCCGTTCCATGGCGAAAAATCACCGAGCTTTC
ATTGCGAGGATCGGAAGGGGCGATATCACTGCTTCGGCTGCGGCGTCTCTGGCGATCATTTTCGCTTCTTGACAGAGCTC
GACGGGATGAGCTTCCCCGAAGCCGTTGAGCGCGTGGCCGAGATGGCGGGCGTGCCGATGCCGGCGCGCGATCCGGAGGC
GGAAAAGCGGGAGAAGGAGCGCGCCAGCCTTACCGATGTCATGGAGATGGCGGCCCAGTTCTTTCAGGACTGCTTGCAAG
GGGCACGAGGAGCCGAGGCAAGAGCCTATTTGCGCGGGCGCGGCATCGCTTCCCTGACCCAGCAGACCTTCCGTCTCGGC
TTTGCGCCCGACAGCCGCAACGCCCTTAAGGAGTTCCTGGCTGGCCGGGGCGTCGGGAAAGAGCAGATGGAGGCTTGCGG
CCTGGTGGTGAGCGGACCGGATGTTCCGGTTTCCTATGACCGCTTCCGCGGCCGCGTCATGTTCCCCATTGAGGACGCGC
GCGGACGCGTCATCGCTTTCGGGGGCCGGGCGCTTTCCTCCGATGTTCCGGCGAAATACCTCAATTCACCGGACACCGAG
CTCTTCCACAAGGGCAATGTGCTCTACAATCACGCCCGCGCGCGCAAGGCCGTGAAGGATGGGCCTGTGATCGCCGTCGA
AGGGTATATGGACGTCATCGCGCTCGCCCAGGCCAGCGTCGAGAATGTCGTGGCGCCGCTGGGCACGGCGCTGACCGAAA
ACCAACTGGAGCTCTTGTGGCGCATCTCGGGCGAGCCGGTTCTCTGTTTCGACGGTGACGGCGCGGGGCTGCGCGCCGCC
TGGCGCGCGGCGGATCTGGCGCTGCCCCTGGTGCAGCCGGGAAGATCGCTGCGCTTCGCGCTTCTCCCCGAAGGCAAGGA
CCCCGATGACCTGGTGCGCGAGGAGGGGAGACAGGCTTTCGATGCGCTCATCGGGGAAACGCGGCCGCTCGCCGATCTCC
TCTGGATGCGCGAGACCTCTGGCCGTGTGTTCGACACGCCGGAGCGGCGCGCCGAATTGGAGCAGACCTTTCGAGAGCTT
GCGAACCGCATCCGCGACGAGAACGTGCGCCGGCATTATGCGCAGGAATTGCGTGAGCGGGTGCAGAGCTTCTTCGGTTC
GGGCCCGCAGCCTGGACGCCAGCAGCGTGGCGGCTTTTCGCCGCGCGGAGGAGGACCGGGCAAGCCTGGCGCGGCGGCCG
GCCGGTTCGCCGTTTCGGAAAGCCTCGCCCGTTCGGCCATGGTGAAGCGCACAGCCGGAATGATGCCGCTTCGCGAGGCT
ACCCTGATCGTCGCCCTCGTAAACCATCCGGCGCTGATCGATGAGCAATTCGACGCCGTCGAGAAGTTGTCGCTCGCCAA
TGCTGATCTCAAAAAGCTCTATTCCGCCCTGCTCGACGCGGTCGCCCATGACGTAGCGCGTGATCATGAAGCGTTGACGG
CGGCGATAGGCGCTGCAGGCCTGGGCGAAATCCTGGAGCGGGCACAGACGCTGGTTCGCCGCGCCAACCAGTGGCCGGCT
CTGCCGGAGGCCGCCTTAGAGGACGCGCGCGAGGCCTTTCTGCAGGCCCTGCACTTGCACCGCAGTGCGGGTGCCCTACA
TAGGGAGCTGAAAGCGGCCGAGCAGGCGCTGGCAAACGAACCGACCGAAGAGAATTATCGGCACCTTGTGGAGATTCAGG
CGGAACTGCGCAACTTGCAGTCGGCGGAAGCGCTCATCGAGGGTTTCGGCATTCTTTCAGGCCGTGTCGGAAGGTCTTGA

Upstream 100 bases:

>100_bases
ACAAGCCTTGGCCGCTAGCCTGTGAACAACGTGCATTGCTCAAACGGGGCCGCGACAGGCCGGCAGGCGGGGTATAAGAT
CGCCATAACAGGCGGGACCG

Downstream 100 bases:

>100_bases
TTTATCCCCAGAAGCGAGCGGTGAAATGCGCTGCGATTCGCTTTTTGGACGCGAATCATGGCACAGCAGCTTGACCTCGG
CGCAATTTAAAGGAATCAGG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MRFPNSFLDEIRDRVPISQVVGSRVTFDKRKSNPGRGDFWACCPFHGEKSPSFHCEDRKGRYHCFGCGVSGDHFRFLTEL
DGMSFPEAVERVAEMAGVPMPARDPEAEKREKERASLTDVMEMAAQFFQDCLQGARGAEARAYLRGRGIASLTQQTFRLG
FAPDSRNALKEFLAGRGVGKEQMEACGLVVSGPDVPVSYDRFRGRVMFPIEDARGRVIAFGGRALSSDVPAKYLNSPDTE
LFHKGNVLYNHARARKAVKDGPVIAVEGYMDVIALAQASVENVVAPLGTALTENQLELLWRISGEPVLCFDGDGAGLRAA
WRAADLALPLVQPGRSLRFALLPEGKDPDDLVREEGRQAFDALIGETRPLADLLWMRETSGRVFDTPERRAELEQTFREL
ANRIRDENVRRHYAQELRERVQSFFGSGPQPGRQQRGGFSPRGGGPGKPGAAAGRFAVSESLARSAMVKRTAGMMPLREA
TLIVALVNHPALIDEQFDAVEKLSLANADLKKLYSALLDAVAHDVARDHEALTAAIGAAGLGEILERAQTLVRRANQWPA
LPEAALEDAREAFLQALHLHRSAGALHRELKAAEQALANEPTEENYRHLVEIQAELRNLQSAEALIEGFGILSGRVGRS

Sequences:

>Translated_639_residues
MRFPNSFLDEIRDRVPISQVVGSRVTFDKRKSNPGRGDFWACCPFHGEKSPSFHCEDRKGRYHCFGCGVSGDHFRFLTEL
DGMSFPEAVERVAEMAGVPMPARDPEAEKREKERASLTDVMEMAAQFFQDCLQGARGAEARAYLRGRGIASLTQQTFRLG
FAPDSRNALKEFLAGRGVGKEQMEACGLVVSGPDVPVSYDRFRGRVMFPIEDARGRVIAFGGRALSSDVPAKYLNSPDTE
LFHKGNVLYNHARARKAVKDGPVIAVEGYMDVIALAQASVENVVAPLGTALTENQLELLWRISGEPVLCFDGDGAGLRAA
WRAADLALPLVQPGRSLRFALLPEGKDPDDLVREEGRQAFDALIGETRPLADLLWMRETSGRVFDTPERRAELEQTFREL
ANRIRDENVRRHYAQELRERVQSFFGSGPQPGRQQRGGFSPRGGGPGKPGAAAGRFAVSESLARSAMVKRTAGMMPLREA
TLIVALVNHPALIDEQFDAVEKLSLANADLKKLYSALLDAVAHDVARDHEALTAAIGAAGLGEILERAQTLVRRANQWPA
LPEAALEDAREAFLQALHLHRSAGALHRELKAAEQALANEPTEENYRHLVEIQAELRNLQSAEALIEGFGILSGRVGRS
>Mature_639_residues
MRFPNSFLDEIRDRVPISQVVGSRVTFDKRKSNPGRGDFWACCPFHGEKSPSFHCEDRKGRYHCFGCGVSGDHFRFLTEL
DGMSFPEAVERVAEMAGVPMPARDPEAEKREKERASLTDVMEMAAQFFQDCLQGARGAEARAYLRGRGIASLTQQTFRLG
FAPDSRNALKEFLAGRGVGKEQMEACGLVVSGPDVPVSYDRFRGRVMFPIEDARGRVIAFGGRALSSDVPAKYLNSPDTE
LFHKGNVLYNHARARKAVKDGPVIAVEGYMDVIALAQASVENVVAPLGTALTENQLELLWRISGEPVLCFDGDGAGLRAA
WRAADLALPLVQPGRSLRFALLPEGKDPDDLVREEGRQAFDALIGETRPLADLLWMRETSGRVFDTPERRAELEQTFREL
ANRIRDENVRRHYAQELRERVQSFFGSGPQPGRQQRGGFSPRGGGPGKPGAAAGRFAVSESLARSAMVKRTAGMMPLREA
TLIVALVNHPALIDEQFDAVEKLSLANADLKKLYSALLDAVAHDVARDHEALTAAIGAAGLGEILERAQTLVRRANQWPA
LPEAALEDAREAFLQALHLHRSAGALHRELKAAEQALANEPTEENYRHLVEIQAELRNLQSAEALIEGFGILSGRVGRS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=424, Percent_Identity=41.0377358490566, Blast_Score=312, Evalue=5e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70222; Mature: 70222

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFPNSFLDEIRDRVPISQVVGSRVTFDKRKSNPGRGDFWACCPFHGEKSPSFHCEDRKG
CCCCHHHHHHHHHCCCHHHHHCCCCCCHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RYHCFGCGVSGDHFRFLTELDGMSFPEAVERVAEMAGVPMPARDPEAEKREKERASLTDV
CEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MEMAAQFFQDCLQGARGAEARAYLRGRGIASLTQQTFRLGFAPDSRNALKEFLAGRGVGK
HHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCH
EQMEACGLVVSGPDVPVSYDRFRGRVMFPIEDARGRVIAFGGRALSSDVPAKYLNSPDTE
HHHHHCCEEECCCCCCCCHHHHCCEEEEEEECCCCCEEEECCCHHCCCCCHHHCCCCCHH
LFHKGNVLYNHARARKAVKDGPVIAVEGYMDVIALAQASVENVVAPLGTALTENQLELLW
HHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
RISGEPVLCFDGDGAGLRAAWRAADLALPLVQPGRSLRFALLPEGKDPDDLVREEGRQAF
EECCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
DALIGETRPLADLLWMRETSGRVFDTPERRAELEQTFRELANRIRDENVRRHYAQELRER
HHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQSFFGSGPQPGRQQRGGFSPRGGGPGKPGAAAGRFAVSESLARSAMVKRTAGMMPLREA
HHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TLIVALVNHPALIDEQFDAVEKLSLANADLKKLYSALLDAVAHDVARDHEALTAAIGAAG
HEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGEILERAQTLVRRANQWPALPEAALEDAREAFLQALHLHRSAGALHRELKAAEQALANE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTEENYRHLVEIQAELRNLQSAEALIEGFGILSGRVGRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRFPNSFLDEIRDRVPISQVVGSRVTFDKRKSNPGRGDFWACCPFHGEKSPSFHCEDRKG
CCCCHHHHHHHHHCCCHHHHHCCCCCCHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RYHCFGCGVSGDHFRFLTELDGMSFPEAVERVAEMAGVPMPARDPEAEKREKERASLTDV
CEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MEMAAQFFQDCLQGARGAEARAYLRGRGIASLTQQTFRLGFAPDSRNALKEFLAGRGVGK
HHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCH
EQMEACGLVVSGPDVPVSYDRFRGRVMFPIEDARGRVIAFGGRALSSDVPAKYLNSPDTE
HHHHHCCEEECCCCCCCCHHHHCCEEEEEEECCCCCEEEECCCHHCCCCCHHHCCCCCHH
LFHKGNVLYNHARARKAVKDGPVIAVEGYMDVIALAQASVENVVAPLGTALTENQLELLW
HHHCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
RISGEPVLCFDGDGAGLRAAWRAADLALPLVQPGRSLRFALLPEGKDPDDLVREEGRQAF
EECCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
DALIGETRPLADLLWMRETSGRVFDTPERRAELEQTFRELANRIRDENVRRHYAQELRER
HHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQSFFGSGPQPGRQQRGGFSPRGGGPGKPGAAAGRFAVSESLARSAMVKRTAGMMPLREA
HHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TLIVALVNHPALIDEQFDAVEKLSLANADLKKLYSALLDAVAHDVARDHEALTAAIGAAG
HEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGEILERAQTLVRRANQWPALPEAALEDAREAFLQALHLHRSAGALHRELKAAEQALANE
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTEENYRHLVEIQAELRNLQSAEALIEGFGILSGRVGRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]