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The map label for this gene is nadE [H]

Identifier: 110633816

GI number: 110633816

Start: 1560766

End: 1562484

Strand: Direct

Name: nadE [H]

Synonym: Meso_1463

Alternate gene names: 110633816

Gene position: 1560766-1562484 (Clockwise)

Preceding gene: 110633815

Following gene: 110633817

Centisome position: 35.37

GC content: 61.61

Gene sequence:

>1719_bases
ATGAGCTTCATGACGAACGCCGCTCCAGACATTTTCCGCATCGCCGTCGCCCAGCTGAATCCCACGGTGGGCGACATCCC
CGGCAATCTCGCCAAGGCGCGCGAGGCGCGCGCGGACGCGGCGCGGAAAGGCGCGGATCTCGTTCTTTTCACCGAGCTTT
TCATCGCCGGCTACCCGCCCGAGGATCTTGTGCTGAAACCGGCCTTTGTCGCAGCCTGCGAGAAGGCGGTGCTGGAACTG
GCTGCCGAGACGGCCGATGGCGGCCCTGGCGTCATCATCGGCACGCCGCTCAAGCGCGAAAGCGGCGTGCACAACGCGGT
CGTGGTGCTCGACGACGGAAAAGTGATCGCCGAGCGCTATAAGCTCGACCTGCCAAATTACGGCGAGTTTGACGAGAAGC
GCGTCTTCGATGCCGCTCCCAGCATGCCCGGCCCGGTGAATTTCCGCGGTGTGCGCATCGGAATTCCCATTTGCGAGGAT
ATCTGGGGCAAGCTCGACGTCTGCGAAACCCTTGCTGAATCCGGTGCCGAAATCCTCCTCGTTCCAAACGGCTCGCCCTA
CTACCGGGGCAAAGTGGAGGTGCGTCAGCAGGTCGTCATCGTGCAGGTGATCGAGAGCGGCCTGCCGATCGTCTATGCCA
ATATGCTTGGCGGGCAGGACGAACTGGTCTTCGACGGCGCTTCCTTTGCGATCCAGGCCGACAAGTCGCTCGCCTTTCAG
ATGAGCCAGTTCGAGGAGGCGGTAGCCGTCACAACCTGGCGCAGAGGCCGCGACGCCGCAGGCGCGCAAAGGGGAAAGGA
CGATGAATGGGTCTGTGCCGAGGGGCCAATGGCGATCATTCCCGAGAAGGAGGAGGCGGACTACCGCGCCTGCATGCTTG
GCCTTCGCGACTATGTGAACAAGAACGGCTTCAAGAATGTGGTGCTGGGCCTTTCCGGGGGCGTCGATTCCGCGCTCTGC
GCGGCGCTTGCCGTTGATGCCTTGGGGGAAGAGCGGGTGAGGGCGGTCATGTTGCCCTATCGATATACATCCAAGGAATC
GCTGCGGGACGCGGAGGCTTGCGCGCGGGCGCTCGGCTGCCGCTATGACATCGTACCCATCAACGAGCCGGTGGAAGGCT
TCAGGAATGCGCTCGGCCAGCTTTTCGAGGGCACCGAAGAGGGTATCACCGAGGAAAACCTCCAGAGCCGGGCGCGCGGC
ACGCTGCTTATGGCGATCTCGAACAAGTTTGGCTCCATGGTGGTAACCACCGGCAACAAGTCTGAAATGTCCGTCGGCTA
CGCCACGCTCTATGGCGACATGAATGGCGGCTTTAATCCGATAAAAGACCTTTATAAAATGCAGGTCTACGCGCTCTGCC
GCTGGCGCAATGCGCATGTGCCGCCGACCGCGCTCGGGCCTTCCGGCGAAGTGATCCCCGCATCGATCATCGACAAGGCG
CCTTCGGCCGAACTGCGCGAGAACCAGACGGATCAGGATTCCCTGCCGCCTTACGCCGTGTTGGATGATATCCTCGAATG
CCTGGTCGAGAATGAATTGGGGGTGGACGAGATCGTCGCGCGCGGACACGATCGCGATCTTGTCCATCGCATCGAGCATC
TTCTTTACGTCGCCGAATACAAGCGCCGTCAGGCAGCACCCGGCGTGAAGATCACAAAGAAGAATTTCGGGCGCGACCGG
CGCTATCCCATCACCAACAAATTCCGCGACCGGGGATGA

Upstream 100 bases:

>100_bases
ATTGGCAGCAGGTGAGCGGGGCAGGCAAAGCACGATCTCTGCGGCGCTGCAGGCTTCCATACGGGCGCCGATCCTTGCAA
GCCTTCCGGACGCAAGCTAG

Downstream 100 bases:

>100_bases
GACAAGCCTTGGCCGCTAGCCTGTGAACAACGTGCATTGCTCAAACGGGGCCGCGACAGGCCGGCAGGCGGGGTATAAGA
TCGCCATAACAGGCGGGACC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MSFMTNAAPDIFRIAVAQLNPTVGDIPGNLAKAREARADAARKGADLVLFTELFIAGYPPEDLVLKPAFVAACEKAVLEL
AAETADGGPGVIIGTPLKRESGVHNAVVVLDDGKVIAERYKLDLPNYGEFDEKRVFDAAPSMPGPVNFRGVRIGIPICED
IWGKLDVCETLAESGAEILLVPNGSPYYRGKVEVRQQVVIVQVIESGLPIVYANMLGGQDELVFDGASFAIQADKSLAFQ
MSQFEEAVAVTTWRRGRDAAGAQRGKDDEWVCAEGPMAIIPEKEEADYRACMLGLRDYVNKNGFKNVVLGLSGGVDSALC
AALAVDALGEERVRAVMLPYRYTSKESLRDAEACARALGCRYDIVPINEPVEGFRNALGQLFEGTEEGITEENLQSRARG
TLLMAISNKFGSMVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALCRWRNAHVPPTALGPSGEVIPASIIDKA
PSAELRENQTDQDSLPPYAVLDDILECLVENELGVDEIVARGHDRDLVHRIEHLLYVAEYKRRQAAPGVKITKKNFGRDR
RYPITNKFRDRG

Sequences:

>Translated_572_residues
MSFMTNAAPDIFRIAVAQLNPTVGDIPGNLAKAREARADAARKGADLVLFTELFIAGYPPEDLVLKPAFVAACEKAVLEL
AAETADGGPGVIIGTPLKRESGVHNAVVVLDDGKVIAERYKLDLPNYGEFDEKRVFDAAPSMPGPVNFRGVRIGIPICED
IWGKLDVCETLAESGAEILLVPNGSPYYRGKVEVRQQVVIVQVIESGLPIVYANMLGGQDELVFDGASFAIQADKSLAFQ
MSQFEEAVAVTTWRRGRDAAGAQRGKDDEWVCAEGPMAIIPEKEEADYRACMLGLRDYVNKNGFKNVVLGLSGGVDSALC
AALAVDALGEERVRAVMLPYRYTSKESLRDAEACARALGCRYDIVPINEPVEGFRNALGQLFEGTEEGITEENLQSRARG
TLLMAISNKFGSMVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALCRWRNAHVPPTALGPSGEVIPASIIDKA
PSAELRENQTDQDSLPPYAVLDDILECLVENELGVDEIVARGHDRDLVHRIEHLLYVAEYKRRQAAPGVKITKKNFGRDR
RYPITNKFRDRG
>Mature_571_residues
SFMTNAAPDIFRIAVAQLNPTVGDIPGNLAKAREARADAARKGADLVLFTELFIAGYPPEDLVLKPAFVAACEKAVLELA
AETADGGPGVIIGTPLKRESGVHNAVVVLDDGKVIAERYKLDLPNYGEFDEKRVFDAAPSMPGPVNFRGVRIGIPICEDI
WGKLDVCETLAESGAEILLVPNGSPYYRGKVEVRQQVVIVQVIESGLPIVYANMLGGQDELVFDGASFAIQADKSLAFQM
SQFEEAVAVTTWRRGRDAAGAQRGKDDEWVCAEGPMAIIPEKEEADYRACMLGLRDYVNKNGFKNVVLGLSGGVDSALCA
ALAVDALGEERVRAVMLPYRYTSKESLRDAEACARALGCRYDIVPINEPVEGFRNALGQLFEGTEEGITEENLQSRARGT
LLMAISNKFGSMVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALCRWRNAHVPPTALGPSGEVIPASIIDKAP
SAELRENQTDQDSLPPYAVLDDILECLVENELGVDEIVARGHDRDLVHRIEHLLYVAEYKRRQAAPGVKITKKNFGRDRR
YPITNKFRDRG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI24641841, Length=599, Percent_Identity=22.7045075125209, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24641843, Length=599, Percent_Identity=22.7045075125209, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 62437; Mature: 62306

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFMTNAAPDIFRIAVAQLNPTVGDIPGNLAKAREARADAARKGADLVLFTELFIAGYPP
CCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCC
EDLVLKPAFVAACEKAVLELAAETADGGPGVIIGTPLKRESGVHNAVVVLDDGKVIAERY
HHCEECHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEEHHH
KLDLPNYGEFDEKRVFDAAPSMPGPVNFRGVRIGIPICEDIWGKLDVCETLAESGAEILL
CCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHCCCEEEE
VPNGSPYYRGKVEVRQQVVIVQVIESGLPIVYANMLGGQDELVFDGASFAIQADKSLAFQ
EECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEEECCCHHHHH
MSQFEEAVAVTTWRRGRDAAGAQRGKDDEWVCAEGPMAIIPEKEEADYRACMLGLRDYVN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHC
KNGFKNVVLGLSGGVDSALCAALAVDALGEERVRAVMLPYRYTSKESLRDAEACARALGC
CCCCCEEEEEECCCHHHHHHHHHHHHHHCHHHHEEEEECCCCCCHHHHHHHHHHHHHHCC
RYDIVPINEPVEGFRNALGQLFEGTEEGITEENLQSRARGTLLMAISNKFGSMVVTTGNK
EEEEEECCCHHHHHHHHHHHHHCCHHCCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCC
SEMSVGYATLYGDMNGGFNPIKDLYKMQVYALCRWRNAHVPPTALGPSGEVIPASIIDKA
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
PSAELRENQTDQDSLPPYAVLDDILECLVENELGVDEIVARGHDRDLVHRIEHLLYVAEY
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
KRRQAAPGVKITKKNFGRDRRYPITNKFRDRG
HHHHCCCCCEEEHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFMTNAAPDIFRIAVAQLNPTVGDIPGNLAKAREARADAARKGADLVLFTELFIAGYPP
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCC
EDLVLKPAFVAACEKAVLELAAETADGGPGVIIGTPLKRESGVHNAVVVLDDGKVIAERY
HHCEECHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEEHHH
KLDLPNYGEFDEKRVFDAAPSMPGPVNFRGVRIGIPICEDIWGKLDVCETLAESGAEILL
CCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHCCCEEEE
VPNGSPYYRGKVEVRQQVVIVQVIESGLPIVYANMLGGQDELVFDGASFAIQADKSLAFQ
EECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEEECCCHHHHH
MSQFEEAVAVTTWRRGRDAAGAQRGKDDEWVCAEGPMAIIPEKEEADYRACMLGLRDYVN
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHC
KNGFKNVVLGLSGGVDSALCAALAVDALGEERVRAVMLPYRYTSKESLRDAEACARALGC
CCCCCEEEEEECCCHHHHHHHHHHHHHHCHHHHEEEEECCCCCCHHHHHHHHHHHHHHCC
RYDIVPINEPVEGFRNALGQLFEGTEEGITEENLQSRARGTLLMAISNKFGSMVVTTGNK
EEEEEECCCHHHHHHHHHHHHHCCHHCCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCC
SEMSVGYATLYGDMNGGFNPIKDLYKMQVYALCRWRNAHVPPTALGPSGEVIPASIIDKA
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCC
PSAELRENQTDQDSLPPYAVLDDILECLVENELGVDEIVARGHDRDLVHRIEHLLYVAEY
CCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
KRRQAAPGVKITKKNFGRDRRYPITNKFRDRG
HHHHCCCCCEEEHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]