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The map label for this gene is narK [H]

Identifier: 110633803

GI number: 110633803

Start: 1543238

End: 1544518

Strand: Direct

Name: narK [H]

Synonym: Meso_1450

Alternate gene names: 110633803

Gene position: 1543238-1544518 (Clockwise)

Preceding gene: 110633802

Following gene: 110633804

Centisome position: 34.97

GC content: 64.48

Gene sequence:

>1281_bases
ATGACCTCACATGAGCCAGCCTCAAGCGCGCCAACCACCACACCGGTCCGCGCGCTCAGCATGTCCACCATCGCCTTTAC
CGTCTGCTTCGCGGTATGGACGATCTTTTCCATCATTGGCGTGCGCATCAAGGAGGAACTGGGCCTCTCGGAAACCCAGT
TCGGCCTGCTTGTGGGCACGCCGATCCTCACCGGCTCGCTGGTGCGCCTTGCGCTCGGCATCTGGACAGACCGCTACGGC
GGCAGGTTGGTCTACACCGTCACTATGCTCGCGGCGGCGATCGCCACCTTTCTCCTCTCCTTCGCCCACACCTATGCGCA
TCTGCTCCTGGCGGGGCTTGGCGTGGGGCTCGCCGGCGGCTCATTCGCCGTGGGCGTCGCCTATGTCTCGCGTTTCTATC
CAGCCCAGAAGCAGGGCACGGCACTTGGCATTTTCGGCGTCGGTAATGTGGGCGCGGCAGTCACGAAATTCCTGGCGCCC
TTTGTGCTCGTCGCCTGGGGCTGGGAAGCGGTGGCGCAGGTTTGGGCGCTGGCGCTCGCTGCAATGGCCATCCTCTTCTG
GTTCACCACGGGCGACGACCCGGTGATCCGCGCCCGGCGGGCAGGGCAGGGTGCGCAGCACAGCTTCGCCGCGGATTTTG
AGCCGCTTAAAAACCTGCAAGTCTGGCGCTTCGCCCTCTACTACTTCTTCGTCTTCGGCGGGTTCGTGGCGCTTTCGCTG
TGGCTGCCGCGCTATCTGATCGGGGTCTACGGGTTCGACATCGCAACAGCCGGCATGATCGGCGCGGCCTATTCCATCCC
CGCCTCGGTCTTCCGCGCCTATGGCGGCGCGCTTTCGGACCGCTCGGGCGCGCGCACCGTGATGTATTGGACGTTTGCCG
TCTCGGCCGTCTGCGCGCTGATCCTGTCCATCCCGCCCACGGACTATGTGGTGCGTGGCGTCAACGGCCCCATCGCAGCC
CATTTTGAGATCGGACCGTGGGCCTTCATCGCCGTTATCTTTGTGCTCGGCTTTTTCATGAGCCTCGGCAAGGCTGCGGT
CTACAAGCACATCCCGGTCTATTACCCGCAAAATGTGGGCGCGGTGGGCGGCCTTGTCGGCATGATCGGCGGCCTGGGCG
GCTTTTTCCTGCCACTTCTCTTCGGCTGGCTCAACGACGTGACCGGCCTTTGGACGAGCTGCTTCATGGCGCTCTTCGTC
ATCGTCGCCGGCTCGCTCGTCTGGATGCACATCGCCATCCGCCAGATGGAGCGCCGCCAAGCGGGCCTGCAGCCCGCCTG
A

Upstream 100 bases:

>100_bases
CACGTTGACCGCGGTGGGCGTGCGAGATCTGGCCGGCAGCTTTTCTTTTGGTCGGCCCCTCCGGGCAGTTTGGACACGAG
CAGTTGGATGGAACCGACCC

Downstream 100 bases:

>100_bases
AAGCGCCACGCGAAACAAAGAGGAAACTTGTTCAAATGACTCAGAAACTCGTGGTGGTCGGCGCCGGCATGGCCTCCGGC
CGCATGCTCGAGCACCTCTT

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTSHEPASSAPTTTPVRALSMSTIAFTVCFAVWTIFSIIGVRIKEELGLSETQFGLLVGTPILTGSLVRLALGIWTDRYG
GRLVYTVTMLAAAIATFLLSFAHTYAHLLLAGLGVGLAGGSFAVGVAYVSRFYPAQKQGTALGIFGVGNVGAAVTKFLAP
FVLVAWGWEAVAQVWALALAAMAILFWFTTGDDPVIRARRAGQGAQHSFAADFEPLKNLQVWRFALYYFFVFGGFVALSL
WLPRYLIGVYGFDIATAGMIGAAYSIPASVFRAYGGALSDRSGARTVMYWTFAVSAVCALILSIPPTDYVVRGVNGPIAA
HFEIGPWAFIAVIFVLGFFMSLGKAAVYKHIPVYYPQNVGAVGGLVGMIGGLGGFFLPLLFGWLNDVTGLWTSCFMALFV
IVAGSLVWMHIAIRQMERRQAGLQPA

Sequences:

>Translated_426_residues
MTSHEPASSAPTTTPVRALSMSTIAFTVCFAVWTIFSIIGVRIKEELGLSETQFGLLVGTPILTGSLVRLALGIWTDRYG
GRLVYTVTMLAAAIATFLLSFAHTYAHLLLAGLGVGLAGGSFAVGVAYVSRFYPAQKQGTALGIFGVGNVGAAVTKFLAP
FVLVAWGWEAVAQVWALALAAMAILFWFTTGDDPVIRARRAGQGAQHSFAADFEPLKNLQVWRFALYYFFVFGGFVALSL
WLPRYLIGVYGFDIATAGMIGAAYSIPASVFRAYGGALSDRSGARTVMYWTFAVSAVCALILSIPPTDYVVRGVNGPIAA
HFEIGPWAFIAVIFVLGFFMSLGKAAVYKHIPVYYPQNVGAVGGLVGMIGGLGGFFLPLLFGWLNDVTGLWTSCFMALFV
IVAGSLVWMHIAIRQMERRQAGLQPA
>Mature_425_residues
TSHEPASSAPTTTPVRALSMSTIAFTVCFAVWTIFSIIGVRIKEELGLSETQFGLLVGTPILTGSLVRLALGIWTDRYGG
RLVYTVTMLAAAIATFLLSFAHTYAHLLLAGLGVGLAGGSFAVGVAYVSRFYPAQKQGTALGIFGVGNVGAAVTKFLAPF
VLVAWGWEAVAQVWALALAAMAILFWFTTGDDPVIRARRAGQGAQHSFAADFEPLKNLQVWRFALYYFFVFGGFVALSLW
LPRYLIGVYGFDIATAGMIGAAYSIPASVFRAYGGALSDRSGARTVMYWTFAVSAVCALILSIPPTDYVVRGVNGPIAAH
FEIGPWAFIAVIFVLGFFMSLGKAAVYKHIPVYYPQNVGAVGGLVGMIGGLGGFFLPLLFGWLNDVTGLWTSCFMALFVI
VAGSLVWMHIAIRQMERRQAGLQPA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=429, Percent_Identity=26.5734265734266, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45625; Mature: 45494

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCC
>Mature Secondary Structure 
TSHEPASSAPTTTPVRALSMSTIAFTVCFAVWTIFSIIGVRIKEELGLSETQFGLLVGT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC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CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]