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The map label for this gene is mrcA [H]

Identifier: 110633784

GI number: 110633784

Start: 1528444

End: 1529346

Strand: Reverse

Name: mrcA [H]

Synonym: Meso_1431

Alternate gene names: 110633784

Gene position: 1529346-1528444 (Counterclockwise)

Preceding gene: 110633786

Following gene: 110633781

Centisome position: 34.66

GC content: 61.57

Gene sequence:

>903_bases
ATGGCAGGCAGGCTCCTGCCGTCTGACGTTTCGGATCGCGCCGCCTCAAGCCCAGTGAAGCGGGCTTTGCGATGGCTTGT
GCTCATGGCGCTTGCCGGGGTGTTCGCGCTTAGCATCCTGATCGCCTGGGCAATGGAAGGCACCCCGTGGAAAGAGATTG
CCGACCAGCCTGCTTCCGCGCAAGCCGGGTATGTTCCCAGATCGGCATTCCCGCGGCATCTTGTCGATGCAGTGCTGGCG
GTGGAGGATCGCCGCTTCTACGGACATTTCGGGCTGGATCTGCGGGGCATTGCCCGGGCCGTCGTCAGCAATTTCCGCGC
CGGCGAGATCGTTCAGGGCGGAAGCACCATCACCCAACAGCTCATAAAGATCGCGTATCTGCAACATGACCGGACATTCA
AACGAAAGTTCCAGGAAGCGGTGATCGCGATCTGGCTGGAACAGAAGCTGGGCAAGGAAGAGATCCTTACCCGCTATCTC
AACAGCATCTATCTCGGCGCCGGCGCCACAGGAATGCCCGCTGCGGCGCGCGTCTATTTCCACAAGGACGTACAAGACAT
CGATCTTGCCGAATCGGCACTTCTTGCGGGCCTGATCCGCGCCCCTTCAATGCTCAATCCGTTGGAAAACCCCGAGGGTG
CCAGAAAACGCGCGGAACTCGTTCTGGACCTCATGGTCATCGACGGCAGGCTGGACGCGGCAGAGGCCGAACAGGCAAAG
CGGCGCTATGCGAAGTTGCGCCTTGCACCGGGCGCTCGCTCGCAGACACTGCCGGCGGCCGGAAACCTGGGCTCGGTCCC
GGAATGCAACATCCAGGCCTGCACGCGGGCATACCGCTCGTTCCGCGCCTCCGACTGCACCTATCAGCCTTATAGTGGCC
CGCGCCAGCTGTGTGAAAAATAG

Upstream 100 bases:

>100_bases
AACGCCGCCTGTTGACAACGCAGGCTCGCGAAAAAGCAAAATCGTCTTACAGCGAGTAATTTTGACCTTACGCAACACCA
TCTTTGAGGGCCGGAAGCGG

Downstream 100 bases:

>100_bases
AACTCACACTTTCGACACGAAATCGGCGGCAATGCGCATTTCCCGCAGCGGACGGACCTTCGTGATCGTCTCGGAATAGA
TCGGATGCGCCTTGTAGGCA

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MAGRLLPSDVSDRAASSPVKRALRWLVLMALAGVFALSILIAWAMEGTPWKEIADQPASAQAGYVPRSAFPRHLVDAVLA
VEDRRFYGHFGLDLRGIARAVVSNFRAGEIVQGGSTITQQLIKIAYLQHDRTFKRKFQEAVIAIWLEQKLGKEEILTRYL
NSIYLGAGATGMPAAARVYFHKDVQDIDLAESALLAGLIRAPSMLNPLENPEGARKRAELVLDLMVIDGRLDAAEAEQAK
RRYAKLRLAPGARSQTLPAAGNLGSVPECNIQACTRAYRSFRASDCTYQPYSGPRQLCEK

Sequences:

>Translated_300_residues
MAGRLLPSDVSDRAASSPVKRALRWLVLMALAGVFALSILIAWAMEGTPWKEIADQPASAQAGYVPRSAFPRHLVDAVLA
VEDRRFYGHFGLDLRGIARAVVSNFRAGEIVQGGSTITQQLIKIAYLQHDRTFKRKFQEAVIAIWLEQKLGKEEILTRYL
NSIYLGAGATGMPAAARVYFHKDVQDIDLAESALLAGLIRAPSMLNPLENPEGARKRAELVLDLMVIDGRLDAAEAEQAK
RRYAKLRLAPGARSQTLPAAGNLGSVPECNIQACTRAYRSFRASDCTYQPYSGPRQLCEK
>Mature_299_residues
AGRLLPSDVSDRAASSPVKRALRWLVLMALAGVFALSILIAWAMEGTPWKEIADQPASAQAGYVPRSAFPRHLVDAVLAV
EDRRFYGHFGLDLRGIARAVVSNFRAGEIVQGGSTITQQLIKIAYLQHDRTFKRKFQEAVIAIWLEQKLGKEEILTRYLN
SIYLGAGATGMPAAARVYFHKDVQDIDLAESALLAGLIRAPSMLNPLENPEGARKRAELVLDLMVIDGRLDAAEAEQAKR
RYAKLRLAPGARSQTLPAAGNLGSVPECNIQACTRAYRSFRASDCTYQPYSGPRQLCEK

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=165, Percent_Identity=43.030303030303, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI87082258, Length=171, Percent_Identity=45.0292397660819, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788867, Length=215, Percent_Identity=33.0232558139535, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1789601, Length=150, Percent_Identity=37.3333333333333, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 32965; Mature: 32834

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRLLPSDVSDRAASSPVKRALRWLVLMALAGVFALSILIAWAMEGTPWKEIADQPASA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
QAGYVPRSAFPRHLVDAVLAVEDRRFYGHFGLDLRGIARAVVSNFRAGEIVQGGSTITQQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEECCCHHHHHH
LIKIAYLQHDRTFKRKFQEAVIAIWLEQKLGKEEILTRYLNSIYLGAGATGMPAAARVYF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
HKDVQDIDLAESALLAGLIRAPSMLNPLENPEGARKRAELVLDLMVIDGRLDAAEAEQAK
HCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RRYAKLRLAPGARSQTLPAAGNLGSVPECNIQACTRAYRSFRASDCTYQPYSGPRQLCEK
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
AGRLLPSDVSDRAASSPVKRALRWLVLMALAGVFALSILIAWAMEGTPWKEIADQPASA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
QAGYVPRSAFPRHLVDAVLAVEDRRFYGHFGLDLRGIARAVVSNFRAGEIVQGGSTITQQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEECCCHHHHHH
LIKIAYLQHDRTFKRKFQEAVIAIWLEQKLGKEEILTRYLNSIYLGAGATGMPAAARVYF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
HKDVQDIDLAESALLAGLIRAPSMLNPLENPEGARKRAELVLDLMVIDGRLDAAEAEQAK
HCCCHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RRYAKLRLAPGARSQTLPAAGNLGSVPECNIQACTRAYRSFRASDCTYQPYSGPRQLCEK
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA