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The map label for this gene is queA

Identifier: 110633758

GI number: 110633758

Start: 1501098

End: 1502174

Strand: Direct

Name: queA

Synonym: Meso_1405

Alternate gene names: 110633758

Gene position: 1501098-1502174 (Clockwise)

Preceding gene: 110633757

Following gene: 110633759

Centisome position: 34.02

GC content: 63.88

Gene sequence:

>1077_bases
ATGCGTGTCGATCTCTTCGACTTCGATCTTCCAGAAGAACGGATCGCGCTGAGGCCCGCAAGCCCGCGCGATTCAGCCCG
TATGCTCGTCGTGCGGCCGGGGAAACCTCTGGAAGATTGCATCGTGCGTGACCTGCCGCAGTTTTTGCGGCCGGGCGATG
CGCTCGTCTTTAACGATACGAAGGTGATCCCGGCCCGGCTTTCAGGCATTCGCCGGCGCGGCGAGACGGTCGCGCAAATC
GAAGCGACGCTGCATATGCGCGCCGGCCCGGACCGCTGGAAGGCATTCCTGAAGCCCGCCAAGCGGGTGGCGGTGGGCGA
GCGCATCCAGTTCGGCCATGATGGCGAAAGCTGTTTTCTGGGTTCACTGGATGCGACGGTGGCGGAAAAAGGCGAGGGTG
GAGAGGCGCTGCTCGTTTTCGATTTCTCCGGGCCGGTTCTCGACGAGGCGATCGCGGCGGCCGGCCATGTGCCGCTGCCG
CCTTATATCGCCTCGAAGCGGCCTGACGACGAGCGCGACCGCGCCGATTACCAGACCATCTACGCGCGCGAGGAAGGCGC
AGTGGCGGCGCCCACCGCCGGGCTCCATTTTACGCCGGATCTCTTCTCAGCCCTCGATACAATCGGCGTCGAGCGGCATT
TCGTGACGCTGCATGTGGGCGCCGGGACCTTCCTGCCGGTGAAGGCGGAGGATACGGAAGCTCACAGGATGCACGCCGAG
TGGGGCGAAGTCGACGGGATGACGGCCGATGCGCTCAACGCGGTGCGTGCACGTGGCGGGTGCATCATCGCCGTGGGCAC
GACGTCGCTCCGGCTGCTCGAAAGCGCGGCTGAGCCTTCGGGGCTGCTCAAGGCCTGGTCCGGCGAGACGGATATCTTCA
TCACACCCGGCTACCGCTTCCGGGCGATTGACATTCTGATGACGAACTTCCATCTGCCTCGCTCGACCCTGTTCATGCTG
GTTTCGGCCTTCAGCGGGCTTGAGACGATGAAATCCGCCTACAGCCATGCCATCGACCAGGGCTACCGGTTTTACTCCTA
CGGCGATTCCAGCCTGCTTTTCAGGGAAGACACGTGA

Upstream 100 bases:

>100_bases
CGGCGGATATTGCGTAGAAGCAGTTAGGGAGTAGCGAATAGCGGGTGGGGACGAGTAGGCTGCACTATTCGCAACTCACT
ATTCGCTCTTTCGCTCACCC

Downstream 100 bases:

>100_bases
GCGACACCTTTACCTTCCGCGTGCTCGCCACCGACGGCAAGGCGCGGCGCGGCGAAATCTCCATGCCGCGCGGCAACATC
CGTACGCCGGCCTTCATGCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MRVDLFDFDLPEERIALRPASPRDSARMLVVRPGKPLEDCIVRDLPQFLRPGDALVFNDTKVIPARLSGIRRRGETVAQI
EATLHMRAGPDRWKAFLKPAKRVAVGERIQFGHDGESCFLGSLDATVAEKGEGGEALLVFDFSGPVLDEAIAAAGHVPLP
PYIASKRPDDERDRADYQTIYAREEGAVAAPTAGLHFTPDLFSALDTIGVERHFVTLHVGAGTFLPVKAEDTEAHRMHAE
WGEVDGMTADALNAVRARGGCIIAVGTTSLRLLESAAEPSGLLKAWSGETDIFITPGYRFRAIDILMTNFHLPRSTLFML
VSAFSGLETMKSAYSHAIDQGYRFYSYGDSSLLFREDT

Sequences:

>Translated_358_residues
MRVDLFDFDLPEERIALRPASPRDSARMLVVRPGKPLEDCIVRDLPQFLRPGDALVFNDTKVIPARLSGIRRRGETVAQI
EATLHMRAGPDRWKAFLKPAKRVAVGERIQFGHDGESCFLGSLDATVAEKGEGGEALLVFDFSGPVLDEAIAAAGHVPLP
PYIASKRPDDERDRADYQTIYAREEGAVAAPTAGLHFTPDLFSALDTIGVERHFVTLHVGAGTFLPVKAEDTEAHRMHAE
WGEVDGMTADALNAVRARGGCIIAVGTTSLRLLESAAEPSGLLKAWSGETDIFITPGYRFRAIDILMTNFHLPRSTLFML
VSAFSGLETMKSAYSHAIDQGYRFYSYGDSSLLFREDT
>Mature_358_residues
MRVDLFDFDLPEERIALRPASPRDSARMLVVRPGKPLEDCIVRDLPQFLRPGDALVFNDTKVIPARLSGIRRRGETVAQI
EATLHMRAGPDRWKAFLKPAKRVAVGERIQFGHDGESCFLGSLDATVAEKGEGGEALLVFDFSGPVLDEAIAAAGHVPLP
PYIASKRPDDERDRADYQTIYAREEGAVAAPTAGLHFTPDLFSALDTIGVERHFVTLHVGAGTFLPVKAEDTEAHRMHAE
WGEVDGMTADALNAVRARGGCIIAVGTTSLRLLESAAEPSGLLKAWSGETDIFITPGYRFRAIDILMTNFHLPRSTLFML
VSAFSGLETMKSAYSHAIDQGYRFYSYGDSSLLFREDT

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=356, Percent_Identity=44.9438202247191, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_MESSB (Q11IH4)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673966.1
- ProteinModelPortal:   Q11IH4
- SMR:   Q11IH4
- STRING:   Q11IH4
- GeneID:   4180997
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1405
- NMPDR:   fig|266779.1.peg.2187
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   Q11IH4
- ProtClustDB:   PRK00147
- BioCyc:   MSP266779:MESO_1405-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39278; Mature: 39278

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFDLPEERIALRPASPRDSARMLVVRPGKPLEDCIVRDLPQFLRPGDALVFNDT
CEEEEECCCCCHHHEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCC
KVIPARLSGIRRRGETVAQIEATLHMRAGPDRWKAFLKPAKRVAVGERIQFGHDGESCFL
EECHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCCCCEEE
GSLDATVAEKGEGGEALLVFDFSGPVLDEAIAAAGHVPLPPYIASKRPDDERDRADYQTI
ECCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHCCCCCCCHHHHCCHHEE
YAREEGAVAAPTAGLHFTPDLFSALDTIGVERHFVTLHVGAGTFLPVKAEDTEAHRMHAE
EEECCCCEECCCCCCEECHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCHHHHHHCC
WGEVDGMTADALNAVRARGGCIIAVGTTSLRLLESAAEPSGLLKAWSGETDIFITPGYRF
CCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCHHHEECCCCCCEEECCCCCE
RAIDILMTNFHLPRSTLFMLVSAFSGLETMKSAYSHAIDQGYRFYSYGDSSLLFREDT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECC
>Mature Secondary Structure
MRVDLFDFDLPEERIALRPASPRDSARMLVVRPGKPLEDCIVRDLPQFLRPGDALVFNDT
CEEEEECCCCCHHHEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCC
KVIPARLSGIRRRGETVAQIEATLHMRAGPDRWKAFLKPAKRVAVGERIQFGHDGESCFL
EECHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEECCCCCCCEEE
GSLDATVAEKGEGGEALLVFDFSGPVLDEAIAAAGHVPLPPYIASKRPDDERDRADYQTI
ECCCCHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCHHCCCCCCCHHHHCCHHEE
YAREEGAVAAPTAGLHFTPDLFSALDTIGVERHFVTLHVGAGTFLPVKAEDTEAHRMHAE
EEECCCCEECCCCCCEECHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCHHHHHHCC
WGEVDGMTADALNAVRARGGCIIAVGTTSLRLLESAAEPSGLLKAWSGETDIFITPGYRF
CCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCHHHEECCCCCCEEECCCCCE
RAIDILMTNFHLPRSTLFMLVSAFSGLETMKSAYSHAIDQGYRFYSYGDSSLLFREDT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA