| Definition | Mesorhizobium sp. BNC1, complete genome. |
|---|---|
| Accession | NC_008254 |
| Length | 4,412,446 |
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The map label for this gene is topA [H]
Identifier: 110633707
GI number: 110633707
Start: 1446096
End: 1448645
Strand: Reverse
Name: topA [H]
Synonym: Meso_1354
Alternate gene names: 110633707
Gene position: 1448645-1446096 (Counterclockwise)
Preceding gene: 110633709
Following gene: 110633706
Centisome position: 32.83
GC content: 62.82
Gene sequence:
>2550_bases ATGGATGTCGTCGTCGTCGAATCGCCTGCAAAGGCGAAAACTATCAACAAATATCTGGGGAAGGATTACAAGGTCCTGGC TTCCTTCGGCCATGTCCGCGATCTCCCGGCTAAGGATGGCTCCGTCCGCCCGGAAGAAGACTTCGCCATGTCCTGGGAGG TCGACAGCCCCTCGGCCAAGAGGCTTGCCGACATTGCAAAGGCGGTCAAGGACGCCGACGGCCTGATCCTCGCCACCGAC CCCGACCGCGAAGGCGAAGCCATCTCCTGGCATGTTCTGGAAGTTCTGCGCCAGAAGCGGGCGCTCAAGGACAAGCCGGT CCGCCGCGTGGTCTTCAACGCCATCACCAAGAAAGCGATCCTCGACGCGATGGCCTCCCCGCGCGAGGTCGACGCTTCGC TGGTAGACGCCTATCTCGCCCGTCGCGCGCTCGATTACCTCGTGGGCTTCACGCTTTCTCCGGTCCTCTGGCGCAAGCTG CCGGGCGCCCGCTCGGCCGGCCGCGTCCAGTCCGTGGCGCTGCGCCTCGTTTGCGACCTCGAGGCGGAGATCGAGCGCTT CGTGCGCGAGGATTACTGGCAGATCGCCGCCCTTCTCGACACGCCGCGTGGCGATAGCTTCGAGGCCCGGCTTACCACGC TGGACGGCAAGAGGCTGCAGAAGCTCGACATCAAAAGTCAGGAAGAAGCGGACTCGATCAAGGCGCTGCTCGACGGTGCG GCCTATCGGGTGCTTTCGGTCGAGGCGAAGCCGACGCGCCGCAACCCTGGCCCGCCGTTCACAACATCCACGCTGCAGCA GGCCGCCTCCTCACGGCTGAGCTTTTCTGCCTCGCGCACCATGCAGGTCGCCCAGCGGCTCTATGAAGGCGTCGAGATCG GCGGGGAAACCACCGGCCTCATCACCTATATGCGTACGGATGGGGTGCAGATGGCGCCCGAGGCCATCGAAGCGGCCCGT TCCGCCGTATCCAACACTTTCGGCCCGCGATACCTGCCCGAAAAGCCGCGCCATTATTCGAGCAAGGCCAAGAACGCGCA GGAAGCGCACGAGGCGATCCGCCCGACCGACTTCTCCCGCACGCCGGACGAGGTGCGCAAATACCTCGATTCCGACCAGG CCAGGCTTTACGAGATGATCTGGAAGCGCGCCATCGCCAGCCAGATGGAGGCGGCGGAGATCGAGCGGACCACTGTCGAG ATCGAGGCTTCGGGCGCTGGCCGCGCGGCGGGACTACGGGCGGTGGGCTCCGTCGTCCGCTTTGACGGCTTTCTTGCGGC CTATGCCGAGGTGAAGGACGAGGACTCCCAGGACGAGGAGGACCGTCGCCTTCCGGAGATCAACAGCGGCGAGGCGCTGA AGCGCAACTCGGTCAATGTCACCAAGCACACAACCGAGCCGCCGCCGCGCTATTCGGAAGCTTCGCTTATCAAGAAGCTC GAGGAACTCGGCATCGGCCGGCCCTCCACCTATGCGGCTATCCTGAAGACGCTGGACGATCGCGGCTATGTCTCCAATGA GAGGCGCAAGCTCGTACCGCAGGCCAAGGGCCGTATCGTTACGGCTTTCCTTGAGAACTTCTTCCGCCGTTATGTGGAAT ATGATTTCACGGCCGACCTTGAGGAAAAGCTGGATGAAATCTCGGACGGTAAGCTTTCGTGGAAACAGGTGTTGCGCGAT TTCTGGAAGGACTTTTCCGCCGCCGTCGGGGATATCAAGGAGCTGCGCGTTACCGATGTGCTCGACGCCCTGAACGAGGA ACTGGCGCCGCTCGTCTTTCCGACGCGTGAGGATGGTGGCGATCCGCGCATCTGCCCCAAATGCGGAGCCGGCAAGCTTT CACTGAAGCTTGGACGATACGGTGCCTTTGTCGGCTGTTCCAACTACCCGGAATGCGACTATACGCGCCAGCTTGGCGAG AACCCGGCGGAAAATGGCGAGGCGGAAGCAGGCCTCAATGGGAACAAGGTGCTGGGCACCGATCCCTTCACCGGCGAAGA GATCACGCTGCGGTCCGGCCGCTTCGGGCCTTACGTCCAAAGGGGCGAAGGGAAAGACGCCAAGCGAGCGGGCCTGCCCA AAGGCTGGACGCCTGAAGATCTCGACCATGAGAAGGCCATCGCCCTGCTTTCACTGCCGCGGGATGTCGGCAAGCATCCG GAAACCGGCAAGATGATTTCCGCCGGCATCGGCCGCTATGGGCCTTTCCTGCTGCATGACGGTACATATGCGAATCTGGA GACGGTCGAAGATGTGTTCTCGGTCGGCCTCAACCGCGCTGTTTCGCTGCTGGCCGAAAAGCAGGCGAAGACCAACGGCG CCCGCGCAAAACCGGCCGCTTTGAAGGAGCTCGGGGAACATCCGGACGGTGGAGTGATTACCGTGCGTGACGGCCGCTTC GGGCCCTATGTCAATGTCGGCAAGGTGAACGCTACTTTGCCCAAGGGCAAGGATCCGATGTCTTTGACCCGCGAGGAGGC GCTGGAACTCCTCGCTGCCAAGGCGGGAAAGACGGGCAAGAAGCCGGCGAAAAGCAAGGCGAGGAGCTGA
Upstream 100 bases:
>100_bases CGCAGCGACAAGCGCGGCCATGTCACGTGCTGCCGGATCGCCGTATTCGGGGCCAACCGCGGCGCAGACCGCGGCATTTA ACCAAGGTATTGCCAGCGAA
Downstream 100 bases:
>100_bases CGGATTTGGCGCGCAAGAACTCGAAAGCGGCAGGAGGGGAAGGTGCTTCCGCCAATCCTCACCTGCCTTCGCGCGAAGAG ATCCTGCGCTTCGTCAGCGA
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 849; Mature: 849
Protein sequence:
>849_residues MDVVVVESPAKAKTINKYLGKDYKVLASFGHVRDLPAKDGSVRPEEDFAMSWEVDSPSAKRLADIAKAVKDADGLILATD PDREGEAISWHVLEVLRQKRALKDKPVRRVVFNAITKKAILDAMASPREVDASLVDAYLARRALDYLVGFTLSPVLWRKL PGARSAGRVQSVALRLVCDLEAEIERFVREDYWQIAALLDTPRGDSFEARLTTLDGKRLQKLDIKSQEEADSIKALLDGA AYRVLSVEAKPTRRNPGPPFTTSTLQQAASSRLSFSASRTMQVAQRLYEGVEIGGETTGLITYMRTDGVQMAPEAIEAAR SAVSNTFGPRYLPEKPRHYSSKAKNAQEAHEAIRPTDFSRTPDEVRKYLDSDQARLYEMIWKRAIASQMEAAEIERTTVE IEASGAGRAAGLRAVGSVVRFDGFLAAYAEVKDEDSQDEEDRRLPEINSGEALKRNSVNVTKHTTEPPPRYSEASLIKKL EELGIGRPSTYAAILKTLDDRGYVSNERRKLVPQAKGRIVTAFLENFFRRYVEYDFTADLEEKLDEISDGKLSWKQVLRD FWKDFSAAVGDIKELRVTDVLDALNEELAPLVFPTREDGGDPRICPKCGAGKLSLKLGRYGAFVGCSNYPECDYTRQLGE NPAENGEAEAGLNGNKVLGTDPFTGEEITLRSGRFGPYVQRGEGKDAKRAGLPKGWTPEDLDHEKAIALLSLPRDVGKHP ETGKMISAGIGRYGPFLLHDGTYANLETVEDVFSVGLNRAVSLLAEKQAKTNGARAKPAALKELGEHPDGGVITVRDGRF GPYVNVGKVNATLPKGKDPMSLTREEALELLAAKAGKTGKKPAKSKARS
Sequences:
>Translated_849_residues MDVVVVESPAKAKTINKYLGKDYKVLASFGHVRDLPAKDGSVRPEEDFAMSWEVDSPSAKRLADIAKAVKDADGLILATD PDREGEAISWHVLEVLRQKRALKDKPVRRVVFNAITKKAILDAMASPREVDASLVDAYLARRALDYLVGFTLSPVLWRKL PGARSAGRVQSVALRLVCDLEAEIERFVREDYWQIAALLDTPRGDSFEARLTTLDGKRLQKLDIKSQEEADSIKALLDGA AYRVLSVEAKPTRRNPGPPFTTSTLQQAASSRLSFSASRTMQVAQRLYEGVEIGGETTGLITYMRTDGVQMAPEAIEAAR SAVSNTFGPRYLPEKPRHYSSKAKNAQEAHEAIRPTDFSRTPDEVRKYLDSDQARLYEMIWKRAIASQMEAAEIERTTVE IEASGAGRAAGLRAVGSVVRFDGFLAAYAEVKDEDSQDEEDRRLPEINSGEALKRNSVNVTKHTTEPPPRYSEASLIKKL EELGIGRPSTYAAILKTLDDRGYVSNERRKLVPQAKGRIVTAFLENFFRRYVEYDFTADLEEKLDEISDGKLSWKQVLRD FWKDFSAAVGDIKELRVTDVLDALNEELAPLVFPTREDGGDPRICPKCGAGKLSLKLGRYGAFVGCSNYPECDYTRQLGE NPAENGEAEAGLNGNKVLGTDPFTGEEITLRSGRFGPYVQRGEGKDAKRAGLPKGWTPEDLDHEKAIALLSLPRDVGKHP ETGKMISAGIGRYGPFLLHDGTYANLETVEDVFSVGLNRAVSLLAEKQAKTNGARAKPAALKELGEHPDGGVITVRDGRF GPYVNVGKVNATLPKGKDPMSLTREEALELLAAKAGKTGKKPAKSKARS >Mature_849_residues MDVVVVESPAKAKTINKYLGKDYKVLASFGHVRDLPAKDGSVRPEEDFAMSWEVDSPSAKRLADIAKAVKDADGLILATD PDREGEAISWHVLEVLRQKRALKDKPVRRVVFNAITKKAILDAMASPREVDASLVDAYLARRALDYLVGFTLSPVLWRKL PGARSAGRVQSVALRLVCDLEAEIERFVREDYWQIAALLDTPRGDSFEARLTTLDGKRLQKLDIKSQEEADSIKALLDGA AYRVLSVEAKPTRRNPGPPFTTSTLQQAASSRLSFSASRTMQVAQRLYEGVEIGGETTGLITYMRTDGVQMAPEAIEAAR SAVSNTFGPRYLPEKPRHYSSKAKNAQEAHEAIRPTDFSRTPDEVRKYLDSDQARLYEMIWKRAIASQMEAAEIERTTVE IEASGAGRAAGLRAVGSVVRFDGFLAAYAEVKDEDSQDEEDRRLPEINSGEALKRNSVNVTKHTTEPPPRYSEASLIKKL EELGIGRPSTYAAILKTLDDRGYVSNERRKLVPQAKGRIVTAFLENFFRRYVEYDFTADLEEKLDEISDGKLSWKQVLRD FWKDFSAAVGDIKELRVTDVLDALNEELAPLVFPTREDGGDPRICPKCGAGKLSLKLGRYGAFVGCSNYPECDYTRQLGE NPAENGEAEAGLNGNKVLGTDPFTGEEITLRSGRFGPYVQRGEGKDAKRAGLPKGWTPEDLDHEKAIALLSLPRDVGKHP ETGKMISAGIGRYGPFLLHDGTYANLETVEDVFSVGLNRAVSLLAEKQAKTNGARAKPAALKELGEHPDGGVITVRDGRF GPYVNVGKVNATLPKGKDPMSLTREEALELLAAKAGKTGKKPAKSKARS
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=613, Percent_Identity=24.7960848287113, Blast_Score=127, Evalue=4e-29, Organism=Homo sapiens, GI4507635, Length=582, Percent_Identity=24.3986254295533, Blast_Score=108, Evalue=2e-23, Organism=Escherichia coli, GI1787529, Length=663, Percent_Identity=40.7239819004525, Blast_Score=463, Evalue=1e-131, Organism=Escherichia coli, GI1788061, Length=550, Percent_Identity=25.8181818181818, Blast_Score=123, Evalue=4e-29, Organism=Caenorhabditis elegans, GI17555378, Length=512, Percent_Identity=27.734375, Blast_Score=122, Evalue=1e-27, Organism=Caenorhabditis elegans, GI32563869, Length=589, Percent_Identity=25.2971137521222, Blast_Score=115, Evalue=7e-26, Organism=Saccharomyces cerevisiae, GI6323263, Length=565, Percent_Identity=23.8938053097345, Blast_Score=105, Evalue=3e-23, Organism=Drosophila melanogaster, GI24585251, Length=606, Percent_Identity=26.2376237623762, Blast_Score=143, Evalue=5e-34, Organism=Drosophila melanogaster, GI24640096, Length=581, Percent_Identity=24.9569707401033, Blast_Score=108, Evalue=2e-23,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 93413; Mature: 93413
Theoretical pI: Translated: 7.89; Mature: 7.89
Prosite motif: PS00396 TOPOISOMERASE_I_PROK ; PS00697 DNA_LIGASE_A1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.8 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDVVVVESPAKAKTINKYLGKDYKVLASFGHVRDLPAKDGSVRPEEDFAMSWEVDSPSAK CCEEEECCCHHHHHHHHHHCCCHHHHHHHCCHHCCCCCCCCCCCCCCCEEEECCCCCHHH RLADIAKAVKDADGLILATDPDREGEAISWHVLEVLRQKRALKDKPVRRVVFNAITKKAI HHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH LDAMASPREVDASLVDAYLARRALDYLVGFTLSPVLWRKLPGARSAGRVQSVALRLVCDL HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC EAEIERFVREDYWQIAALLDTPRGDSFEARLTTLDGKRLQKLDIKSQEEADSIKALLDGA HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHCH AYRVLSVEAKPTRRNPGPPFTTSTLQQAASSRLSFSASRTMQVAQRLYEGVEIGGETTGL HEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE ITYMRTDGVQMAPEAIEAARSAVSNTFGPRYLPEKPRHYSSKAKNAQEAHEAIRPTDFSR EEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCCCCCC TPDEVRKYLDSDQARLYEMIWKRAIASQMEAAEIERTTVEIEASGAGRAAGLRAVGSVVR CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHH FDGFLAAYAEVKDEDSQDEEDRRLPEINSGEALKRNSVNVTKHTTEPPPRYSEASLIKKL HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHH EELGIGRPSTYAAILKTLDDRGYVSNERRKLVPQAKGRIVTAFLENFFRRYVEYDFTADL HHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCCCCH EEKLDEISDGKLSWKQVLRDFWKDFSAAVGDIKELRVTDVLDALNEELAPLVFPTREDGG HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC DPRICPKCGAGKLSLKLGRYGAFVGCSNYPECDYTRQLGENPAENGEAEAGLNGNKVLGT CCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEECC DPFTGEEITLRSGRFGPYVQRGEGKDAKRAGLPKGWTPEDLDHEKAIALLSLPRDVGKHP CCCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCC ETGKMISAGIGRYGPFLLHDGTYANLETVEDVFSVGLNRAVSLLAEKQAKTNGARAKPAA CCCCHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH LKELGEHPDGGVITVRDGRFGPYVNVGKVNATLPKGKDPMSLTREEALELLAAKAGKTGK HHHHCCCCCCCEEEEECCCCCCCEEECCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC KPAKSKARS CCCHHCCCC >Mature Secondary Structure MDVVVVESPAKAKTINKYLGKDYKVLASFGHVRDLPAKDGSVRPEEDFAMSWEVDSPSAK CCEEEECCCHHHHHHHHHHCCCHHHHHHHCCHHCCCCCCCCCCCCCCCEEEECCCCCHHH RLADIAKAVKDADGLILATDPDREGEAISWHVLEVLRQKRALKDKPVRRVVFNAITKKAI HHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH LDAMASPREVDASLVDAYLARRALDYLVGFTLSPVLWRKLPGARSAGRVQSVALRLVCDL HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC EAEIERFVREDYWQIAALLDTPRGDSFEARLTTLDGKRLQKLDIKSQEEADSIKALLDGA HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCHHHHHHHHHHHHCH AYRVLSVEAKPTRRNPGPPFTTSTLQQAASSRLSFSASRTMQVAQRLYEGVEIGGETTGL HEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE ITYMRTDGVQMAPEAIEAARSAVSNTFGPRYLPEKPRHYSSKAKNAQEAHEAIRPTDFSR EEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCCCCCC TPDEVRKYLDSDQARLYEMIWKRAIASQMEAAEIERTTVEIEASGAGRAAGLRAVGSVVR CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHH FDGFLAAYAEVKDEDSQDEEDRRLPEINSGEALKRNSVNVTKHTTEPPPRYSEASLIKKL HHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHH EELGIGRPSTYAAILKTLDDRGYVSNERRKLVPQAKGRIVTAFLENFFRRYVEYDFTADL HHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCCCCH EEKLDEISDGKLSWKQVLRDFWKDFSAAVGDIKELRVTDVLDALNEELAPLVFPTREDGG HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCC DPRICPKCGAGKLSLKLGRYGAFVGCSNYPECDYTRQLGENPAENGEAEAGLNGNKVLGT CCCCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEECC DPFTGEEITLRSGRFGPYVQRGEGKDAKRAGLPKGWTPEDLDHEKAIALLSLPRDVGKHP CCCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCC ETGKMISAGIGRYGPFLLHDGTYANLETVEDVFSVGLNRAVSLLAEKQAKTNGARAKPAA CCCCHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH LKELGEHPDGGVITVRDGRFGPYVNVGKVNATLPKGKDPMSLTREEALELLAAKAGKTGK HHHHCCCCCCCEEEEECCCCCCCEEECCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCC KPAKSKARS CCCHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA