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The map label for this gene is livJ [C]

Identifier: 110633641

GI number: 110633641

Start: 1380502

End: 1381527

Strand: Reverse

Name: livJ [C]

Synonym: Meso_1288

Alternate gene names: 110633641

Gene position: 1381527-1380502 (Counterclockwise)

Preceding gene: 110633649

Following gene: 110633640

Centisome position: 31.31

GC content: 65.01

Gene sequence:

>1026_bases
ATGGGGACACTGTCGGCGCGCTCCCAGGAACTGGTGATCGGGCTTTCCGCGCCGCTGAGCGGCGATTTCCAGTTGCTTGG
GGAACAGTGGGCCGATGGCGCGCGAGCGGCGGCAACGCGGGACCAGTCCACGCAACTGGTCGTGACCGACGATGCCTGCA
CGGCGGAAGGGGGAGCGGCAGCCGCGCGGCAGCTGGTGGATGCGCAGGTCACGATCGTGATCGGCTTTCTCTGCACGCCG
GCGATCGAAGCTGCCATGCCGATCCTGAAAGAGGCCGGCATTCCGGTCATCACGCCTGTCAGAACCAGCAGCCTCACGGA
CCAGAAGGACCGTACCGGCTGGCCCGTCTACCGGCTGGGACCGCGGGCCGATGACGAGCGGGAGGCCGTGGCAAGTCTTC
TCGTCCCGCTATGGCGGGAAGAATTCTTTGCAATCGTGGACGACGGCACCATTCATGGCCGGGAGCTTGCGGAGAACCTG
CGGGCCGAGGCGGAGCTTGCGAAGCTGAAGCCTGTTCTTGTCGATACATTTCGCCCGCAGATGGACAATCAGATCGGCCT
GGTCGGCAGATTGCGGAGAGCAGGCGCCGCTTATGTCTTCGTCGGTGGCGATCGGAGCGATGTCGCCATCATGGCGCGCG
ACGCGGGCAATCTTGATTACTCCGTCACCTTCGCGGGGGGCGAGGCTCTCAGATCGGCGGATGGACCCGTCCCCCTGCCG
GAAGGAGTGTTGATGGTCGGGCTGCCGGAATGGCGGGACCTTGCGACCCCTGAGGCAAACGAAGTGCTGCAGCGCGCGGG
CGTCCTGGCGGAGGGCTATACCCTTCCCGGCCTGGCCGCGTTCGAGATCGCGGCAAAGGCCAGCCGGGAGGCGATGCAGG
CCGGGGTGGAGATGACGGACGCACTCTCACGCCTCAGCTTCAGCACCGGCATGGGAGAGATCCACTTCAATGAGAAGGGG
GATCTCGCCGACAATCCCTATCGGCTCTTCCGCTATGACGGAGAGCAATTCGTGGAGACGGATTGA

Upstream 100 bases:

>100_bases
CCTGCCAAGCTTTACCAGTGCAACGGCTACCGCTGCATAACAGCCTCGGCTTCAAAATGTGATGCGCCTGGTTACATTCA
CCTTGCTTCTTTTCTCCGCG

Downstream 100 bases:

>100_bases
GTGTTCAAAACCGGCGCCCGAAATCTCATCACCGATGTTCCCGGCCTTCGCGTCGGAAACAGCGAGGATCAAACTCTCAA
ATCAGGGGTGACGGTCGTCG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MGTLSARSQELVIGLSAPLSGDFQLLGEQWADGARAAATRDQSTQLVVTDDACTAEGGAAAARQLVDAQVTIVIGFLCTP
AIEAAMPILKEAGIPVITPVRTSSLTDQKDRTGWPVYRLGPRADDEREAVASLLVPLWREEFFAIVDDGTIHGRELAENL
RAEAELAKLKPVLVDTFRPQMDNQIGLVGRLRRAGAAYVFVGGDRSDVAIMARDAGNLDYSVTFAGGEALRSADGPVPLP
EGVLMVGLPEWRDLATPEANEVLQRAGVLAEGYTLPGLAAFEIAAKASREAMQAGVEMTDALSRLSFSTGMGEIHFNEKG
DLADNPYRLFRYDGEQFVETD

Sequences:

>Translated_341_residues
MGTLSARSQELVIGLSAPLSGDFQLLGEQWADGARAAATRDQSTQLVVTDDACTAEGGAAAARQLVDAQVTIVIGFLCTP
AIEAAMPILKEAGIPVITPVRTSSLTDQKDRTGWPVYRLGPRADDEREAVASLLVPLWREEFFAIVDDGTIHGRELAENL
RAEAELAKLKPVLVDTFRPQMDNQIGLVGRLRRAGAAYVFVGGDRSDVAIMARDAGNLDYSVTFAGGEALRSADGPVPLP
EGVLMVGLPEWRDLATPEANEVLQRAGVLAEGYTLPGLAAFEIAAKASREAMQAGVEMTDALSRLSFSTGMGEIHFNEKG
DLADNPYRLFRYDGEQFVETD
>Mature_340_residues
GTLSARSQELVIGLSAPLSGDFQLLGEQWADGARAAATRDQSTQLVVTDDACTAEGGAAAARQLVDAQVTIVIGFLCTPA
IEAAMPILKEAGIPVITPVRTSSLTDQKDRTGWPVYRLGPRADDEREAVASLLVPLWREEFFAIVDDGTIHGRELAENLR
AEAELAKLKPVLVDTFRPQMDNQIGLVGRLRRAGAAYVFVGGDRSDVAIMARDAGNLDYSVTFAGGEALRSADGPVPLPE
GVLMVGLPEWRDLATPEANEVLQRAGVLAEGYTLPGLAAFEIAAKASREAMQAGVEMTDALSRLSFSTGMGEIHFNEKGD
LADNPYRLFRYDGEQFVETD

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=345, Percent_Identity=24.6376811594203, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789867, Length=237, Percent_Identity=22.7848101265823, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 36535; Mature: 36403

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLSARSQELVIGLSAPLSGDFQLLGEQWADGARAAATRDQSTQLVVTDDACTAEGGAA
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCHH
AARQLVDAQVTIVIGFLCTPAIEAAMPILKEAGIPVITPVRTSSLTDQKDRTGWPVYRLG
HHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCEEEEC
PRADDEREAVASLLVPLWREEFFAIVDDGTIHGRELAENLRAEAELAKLKPVLVDTFRPQ
CCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCC
MDNQIGLVGRLRRAGAAYVFVGGDRSDVAIMARDAGNLDYSVTFAGGEALRSADGPVPLP
CCCCCCHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCEEEEEECCCHHHHCCCCCCCCC
EGVLMVGLPEWRDLATPEANEVLQRAGVLAEGYTLPGLAAFEIAAKASREAMQAGVEMTD
CCEEEEECCCHHHCCCCCHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
ALSRLSFSTGMGEIHFNEKGDLADNPYRLFRYDGEQFVETD
HHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCHHHCCCC
>Mature Secondary Structure 
GTLSARSQELVIGLSAPLSGDFQLLGEQWADGARAAATRDQSTQLVVTDDACTAEGGAA
CCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCHH
AARQLVDAQVTIVIGFLCTPAIEAAMPILKEAGIPVITPVRTSSLTDQKDRTGWPVYRLG
HHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCEEEEC
PRADDEREAVASLLVPLWREEFFAIVDDGTIHGRELAENLRAEAELAKLKPVLVDTFRPQ
CCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCC
MDNQIGLVGRLRRAGAAYVFVGGDRSDVAIMARDAGNLDYSVTFAGGEALRSADGPVPLP
CCCCCCHHHHHHHCCEEEEEECCCCCCEEEEEECCCCCEEEEEECCCHHHHCCCCCCCCC
EGVLMVGLPEWRDLATPEANEVLQRAGVLAEGYTLPGLAAFEIAAKASREAMQAGVEMTD
CCEEEEECCCHHHCCCCCHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
ALSRLSFSTGMGEIHFNEKGDLADNPYRLFRYDGEQFVETD
HHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]