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The map label for this gene is gcvH [H]

Identifier: 110633371

GI number: 110633371

Start: 1117268

End: 1117636

Strand: Reverse

Name: gcvH [H]

Synonym: Meso_1017

Alternate gene names: 110633371

Gene position: 1117636-1117268 (Counterclockwise)

Preceding gene: 110633372

Following gene: 110633370

Centisome position: 25.33

GC content: 60.16

Gene sequence:

>369_bases
ATGAGCAAGACCTATTTCACAGAAGACCACGAGTGGATCCGCGTCGAAGGAGAGACGGCGACCGTGGGCATCACCGATTA
TGCCCAGGAGCAACTGGGCGATCTCGTCTTCGTCGAGCTGCCCGAAACGGGCAAGACGCTCAAGAAGGGAGACGCGGCGG
TGGTGGTTGAATCCGTGAAGGCCGCCTCCGATGTCTATGCTCCAGTGGACGGCGAAGTGACCGAAGTGAACGAAGCGCTG
TCCAGCGAACCCGCTCTCGTCAACGGCGCCCCGATGGATGGCGGCTGGCTGTGGAAGATGAAGCTTTCCGACAAAAGCCA
GCTCGAAGGCCTTATGGACGAGGCAGGCTACAAGGCGACGATCGCCTAG

Upstream 100 bases:

>100_bases
CGGGAACCATGCTCGTGGCGGAGGTGCGCGGAAACCGCCTGCCGATTGCCGTTCACCCCCTGCCCTTCACGCCCCATCGC
TATCGCAAAGGATAAAAAGA

Downstream 100 bases:

>100_bases
GAGAATCATTTATGACTGCAACTTTCTCCACCCGCCATATCGGCCCGCGCAGCGACGAGACCAAGGCGATGCTCGCCTTT
CTCGGTCTCCCCTCGCTCGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 121

Protein sequence:

>122_residues
MSKTYFTEDHEWIRVEGETATVGITDYAQEQLGDLVFVELPETGKTLKKGDAAVVVESVKAASDVYAPVDGEVTEVNEAL
SSEPALVNGAPMDGGWLWKMKLSDKSQLEGLMDEAGYKATIA

Sequences:

>Translated_122_residues
MSKTYFTEDHEWIRVEGETATVGITDYAQEQLGDLVFVELPETGKTLKKGDAAVVVESVKAASDVYAPVDGEVTEVNEAL
SSEPALVNGAPMDGGWLWKMKLSDKSQLEGLMDEAGYKATIA
>Mature_121_residues
SKTYFTEDHEWIRVEGETATVGITDYAQEQLGDLVFVELPETGKTLKKGDAAVVVESVKAASDVYAPVDGEVTEVNEALS
SEPALVNGAPMDGGWLWKMKLSDKSQLEGLMDEAGYKATIA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=51.2396694214876, Blast_Score=127, Evalue=2e-30,
Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=51.2396694214876, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1789271, Length=117, Percent_Identity=56.4102564102564, Blast_Score=139, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17551294, Length=112, Percent_Identity=50.8928571428571, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=50.8620689655172, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=47.5, Blast_Score=107, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=50, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13171; Mature: 13040

Theoretical pI: Translated: 4.01; Mature: 4.01

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTYFTEDHEWIRVEGETATVGITDYAQEQLGDLVFVELPETGKTLKKGDAAVVVESVK
CCCCEECCCCCEEEEECCEEEEEEHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEEHHH
AASDVYAPVDGEVTEVNEALSSEPALVNGAPMDGGWLWKMKLSDKSQLEGLMDEAGYKAT
HHCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHCCCEEEE
IA
CC
>Mature Secondary Structure 
SKTYFTEDHEWIRVEGETATVGITDYAQEQLGDLVFVELPETGKTLKKGDAAVVVESVK
CCCEECCCCCEEEEECCEEEEEEHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEEEHHH
AASDVYAPVDGEVTEVNEALSSEPALVNGAPMDGGWLWKMKLSDKSQLEGLMDEAGYKAT
HHCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHCCCEEEE
IA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]