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The map label for this gene is folE

Identifier: 110633358

GI number: 110633358

Start: 1101739

End: 1102407

Strand: Reverse

Name: folE

Synonym: Meso_1004

Alternate gene names: 110633358

Gene position: 1102407-1101739 (Counterclockwise)

Preceding gene: 110633362

Following gene: 110633357

Centisome position: 24.98

GC content: 64.28

Gene sequence:

>669_bases
ATGGACGCGATCATCAAGAGCTTCCCGCCTCGTCCTGACCAGACCTCCGTACCGGCGGAACAGGCCGGCTATCTCGACAA
GCCGATCACGGACCGGCCCAGCCGGGAGGAGGCCGAGGCCGCCGTGCGAACGCTTCTGCGCTGGGCCGGCGACAATCCGG
ACCGCGAGGGCCTCAAGGAGACGCCAGCGCGTGTGGTGAAAGCGTACCAGGAATTGTTCGGCGGCTATGATCTCTGTCCG
GCCGAGGAACTCGGGCGCACCTTCGAGGAGGTCGCCGGCTATGAGGACATGGTGCTCGTGAAGGACATCCCCTTTCACTC
CCACTGCGAGCATCACATGGTGCCGATCGTCGGTAAGGCCCATGTGGGCTACTTGCCGGACGGAAAGGTCGTCGGCCTGT
CTAAGATCGCGCGCGTTGTGGACATCTTCGCCCGCCGGCTTCAAACCCAGGAAGCGATAACGGCCCAGATCGCCGGCGCG
ATCCAGAACGTGCTCCAGCCCCTCGGCGTCGCCGTGATGGTCGAGGCGGAGCACATGTGCATGGCCATGCGCGGCGTCCG
CAAGCAGGGCGCCAGCACGCTTACGACAACATTTACCGGAGCCTTCAAGACGCGACCTGAAGACCAGGCGCGCTTTATCA
CCATGGTCCGCGATTCGCGCAGCGGTTAA

Upstream 100 bases:

>100_bases
CGCTTTCGGGCGGGACTGATTGCAGTTCCCAACAACCCGCAAAAGGCAGTGACCGTTTAACTCGCTCCTGCGCGACAGGG
GCTACGGGAGACGCCTTAAC

Downstream 100 bases:

>100_bases
GCCATGCAAGCCTCGGAACTTCGCTTCGCGCCTCCCTCAGCCGACAAAAGCGCGCTGGAGGAAGGCAGGCTTTTTACGCC
GCGCTTCGATGCGAACGGCC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MDAIIKSFPPRPDQTSVPAEQAGYLDKPITDRPSREEAEAAVRTLLRWAGDNPDREGLKETPARVVKAYQELFGGYDLCP
AEELGRTFEEVAGYEDMVLVKDIPFHSHCEHHMVPIVGKAHVGYLPDGKVVGLSKIARVVDIFARRLQTQEAITAQIAGA
IQNVLQPLGVAVMVEAEHMCMAMRGVRKQGASTLTTTFTGAFKTRPEDQARFITMVRDSRSG

Sequences:

>Translated_222_residues
MDAIIKSFPPRPDQTSVPAEQAGYLDKPITDRPSREEAEAAVRTLLRWAGDNPDREGLKETPARVVKAYQELFGGYDLCP
AEELGRTFEEVAGYEDMVLVKDIPFHSHCEHHMVPIVGKAHVGYLPDGKVVGLSKIARVVDIFARRLQTQEAITAQIAGA
IQNVLQPLGVAVMVEAEHMCMAMRGVRKQGASTLTTTFTGAFKTRPEDQARFITMVRDSRSG
>Mature_222_residues
MDAIIKSFPPRPDQTSVPAEQAGYLDKPITDRPSREEAEAAVRTLLRWAGDNPDREGLKETPARVVKAYQELFGGYDLCP
AEELGRTFEEVAGYEDMVLVKDIPFHSHCEHHMVPIVGKAHVGYLPDGKVVGLSKIARVVDIFARRLQTQEAITAQIAGA
IQNVLQPLGVAVMVEAEHMCMAMRGVRKQGASTLTTTFTGAFKTRPEDQARFITMVRDSRSG

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=218, Percent_Identity=44.954128440367, Blast_Score=180, Evalue=7e-46,
Organism=Homo sapiens, GI4503949, Length=218, Percent_Identity=44.954128440367, Blast_Score=180, Evalue=7e-46,
Organism=Homo sapiens, GI66932970, Length=187, Percent_Identity=43.3155080213904, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI66932972, Length=177, Percent_Identity=45.1977401129944, Blast_Score=135, Evalue=2e-32,
Organism=Escherichia coli, GI1788476, Length=163, Percent_Identity=41.7177914110429, Blast_Score=116, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=46.0674157303371, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321706, Length=186, Percent_Identity=45.1612903225806, Blast_Score=169, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24656782, Length=190, Percent_Identity=47.3684210526316, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24656796, Length=190, Percent_Identity=47.3684210526316, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24656791, Length=190, Percent_Identity=47.3684210526316, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_MESSB (Q11JM4)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673566.1
- ProteinModelPortal:   Q11JM4
- SMR:   Q11JM4
- STRING:   Q11JM4
- GeneID:   4180518
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_1004
- NMPDR:   fig|266779.1.peg.3975
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   LTTTFTG
- PhylomeDB:   Q11JM4
- ProtClustDB:   PRK09347
- BioCyc:   MSP266779:MESO_1004-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 24476; Mature: 24476

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAIIKSFPPRPDQTSVPAEQAGYLDKPITDRPSREEAEAAVRTLLRWAGDNPDREGLKE
CCCCHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
TPARVVKAYQELFGGYDLCPAEELGRTFEEVAGYEDMVLVKDIPFHSHCEHHMVPIVGKA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEECCC
HVGYLPDGKVVGLSKIARVVDIFARRLQTQEAITAQIAGAIQNVLQPLGVAVMVEAEHMC
CCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHH
MAMRGVRKQGASTLTTTFTGAFKTRPEDQARFITMVRDSRSG
HHHHHHHHHCCCHHHHHHCCHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDAIIKSFPPRPDQTSVPAEQAGYLDKPITDRPSREEAEAAVRTLLRWAGDNPDREGLKE
CCCCHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHH
TPARVVKAYQELFGGYDLCPAEELGRTFEEVAGYEDMVLVKDIPFHSHCEHHMVPIVGKA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEECCC
HVGYLPDGKVVGLSKIARVVDIFARRLQTQEAITAQIAGAIQNVLQPLGVAVMVEAEHMC
CCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHH
MAMRGVRKQGASTLTTTFTGAFKTRPEDQARFITMVRDSRSG
HHHHHHHHHCCCHHHHHHCCHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA