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The map label for this gene is mrcA [H]

Identifier: 110633240

GI number: 110633240

Start: 974771

End: 976915

Strand: Reverse

Name: mrcA [H]

Synonym: Meso_0883

Alternate gene names: 110633240

Gene position: 976915-974771 (Counterclockwise)

Preceding gene: 110633241

Following gene: 110633239

Centisome position: 22.14

GC content: 63.08

Gene sequence:

>2145_bases
ATGGTAGAGAAGCGTACGCCGAAGAAGGGAAATACACCGAGGGCCACGATCTTCCTCGACATAGATGCGTGGATCGATTC
CACCCTCTATGAGCTGCGTTTCAAAGCCGCCGAGTTCTGGGAAGACCTCACCGTCTTCTTCCGCCGCTTCCGCTTCTACG
GCTTAAGGCGCGCCGTCATCGAATTCTTCAGCGAAGGGGTCACCATGCTGGCCGCGGGCTCGGTGGTTATGCTGGGTCTT
GCCATACCCGCCTTCCAGGAGACAGCGGGCGACTGGCGCGCGCAGGACGATTATGCCGTCACCTTTCTTGACCGCTACGG
GCAGGAGATCGGCCAGCGCGGCATCCGCCAGCGCGATTCCGTGCCGGTCGACGAGATGCCCGACCATGTGGTCAAGGCTG
TCCTGGCCACGGAAGACCGGCGCTTCTTCGAGCATTTCGGGATTGATTTCGTCGGCCTGTTCCGCGCGCTTTCTGAGAAC
ATGCGCGCCAATTCCGTGGTGCAGGGCGGCTCCACCATCACGCAGCAGCTTGCGAAAAACCTGTTCCTCACCAATGAGCG
CACGGTGGAGCGCAAGATCAAGGAAGCATTTCTCGCCGTCTGGCTGGAAGCCAATCTGACGAAAAAAGAGATCCTGCAGC
TTTATCTCGACCGCGCTTATCTCGGCGGCGGCACTTTCGGCATCTCCGCCGCGGCCGATTTCTATTTCGGCAAGCAGGTC
AAGGACCTCACATTGCCCGAGGCGGCCATGATCGCCGGCCTCTTCAAGGCTCCCGCGCGCTATGCGCCGCATATCAATCT
TCCCGCCGCGCGCGCCCGCGCCAATGAGGTGCTCAGCAATCTCGTCCAGTCCCGCTTCATGACGGAGGGGCAGGTGCTTT
CGGCCCGGCTCAATCCGGCGACTGCGGTGGACCGCGGCGACCGCGAGGCGCCCGACTATTTCCTGGATTGGGCGTTCGAG
GAGGTGAAGAAGATCGTGCCGGAGGGCGCAACCCATTCGCTGGTGGCGCGCACGACCATGGACCTTGACCTGCAGCATGC
GGTCGAGGAATCGATCGAATTTCATCTGCGCCAATACGGCAAGGATTACGACGTGGAGGAAGGCGCCGCTATCCTCATGG
ATACGGACGGCGCGGTACGCGCGCTGGTCGGCGGGCGGGACTACGGCCAGAGCCAGTTCAACCGCGCAACGCGCGCCGCG
CGCCAGCCAGGTTCCTCCTTCAAGCCCTATGTCTACGCGGCCGCCATGGAAGCGGGTTTGACGCCGAAGACCGTTGTTTC
CGACGCGCCCATATCCTGGGGCAACTGGTCGCCGCAGAATTACGGGCGCAGCTATGCCGGCCGCGTCGACCTCGCGACAG
CGCTCGCAAGGTCCTACAACACGGTCCCCGTCCGCCTGGCGCGCGACCATCTGGGCATCGATGTCATCCAGAAGCTGGTG
AAAGATATGGGCGTGGAAACGCCGCTCAACGGCCATAAGACCATGGTGCTGGGCACGTCCGATCTGACGGTGCTGGATCA
GGTGACCGGATACAGCGTTTTCGCCAATGGCGGCTTTGCCGGCACACGTCACGGCATCCTGGAACTGCATACTCACACCG
GCGAACTCGTTTACGATTGGCGGCGCGATGCGCCGCCGCCACGCCGCGTCCTTTCCGAAACGGCCGTTTCGAACATGAAC
AGCATGCTGGTTGAGGTGCCGGAGCGCGGCACGGCCCGGCGCGCGGCCCTTCCCAATATCCGCTCCGCGGGGAAAACCGG
CACGACCCAGTCCTATCGCGATGCCTGGTATGTCGGTTACACCGGAAATTATGTCGCCGCCGTCTGGCTCGGCAATGACG
ATTACAGGCCAACCGACAATATGACCGGCGGCACGCTGCCGGCCATGGTCTGGCAACGCTTCATGGCCTATGCCCACCAG
AACCTCGAGTTGAAACCTATTCCGGGCATCGAAAATCCGCTGCCCGCCAAGAGCGACGAGGCCGTGGCGGCTGCCAAAAC
CGAGGGTGTGGAGGCCGGTCCGCGCCGGCCGGCGCATAGCCTGCCGCTTGCCACCCGCACCGTGCTGAACGATCTGCTCG
GGCTCTTCCGCCAGGCGCCCCCCGTCAAGCCTCGCCGCGTCTCCGAAAACCTGTCGGCTCTTTGA

Upstream 100 bases:

>100_bases
ACCAAATGTGTTAAGCTTAGCTGGGGTAGGCTCGCGCGTACCTAAAGCCGCGTTGTTTCGCGGTGCAGCGAGAGCAGGAA
GGACGCCGGGCTGTTCGGTC

Downstream 100 bases:

>100_bases
TTGTAAAACCCAGCTCGGTTTTTACTGTTACGAGTTGCGCCGCCTCGGTCCTCTGCGTATTCGATAAATTCATTTCCGAT
ATGGGTTTCGATGCTAAGAA

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MVEKRTPKKGNTPRATIFLDIDAWIDSTLYELRFKAAEFWEDLTVFFRRFRFYGLRRAVIEFFSEGVTMLAAGSVVMLGL
AIPAFQETAGDWRAQDDYAVTFLDRYGQEIGQRGIRQRDSVPVDEMPDHVVKAVLATEDRRFFEHFGIDFVGLFRALSEN
MRANSVVQGGSTITQQLAKNLFLTNERTVERKIKEAFLAVWLEANLTKKEILQLYLDRAYLGGGTFGISAAADFYFGKQV
KDLTLPEAAMIAGLFKAPARYAPHINLPAARARANEVLSNLVQSRFMTEGQVLSARLNPATAVDRGDREAPDYFLDWAFE
EVKKIVPEGATHSLVARTTMDLDLQHAVEESIEFHLRQYGKDYDVEEGAAILMDTDGAVRALVGGRDYGQSQFNRATRAA
RQPGSSFKPYVYAAAMEAGLTPKTVVSDAPISWGNWSPQNYGRSYAGRVDLATALARSYNTVPVRLARDHLGIDVIQKLV
KDMGVETPLNGHKTMVLGTSDLTVLDQVTGYSVFANGGFAGTRHGILELHTHTGELVYDWRRDAPPPRRVLSETAVSNMN
SMLVEVPERGTARRAALPNIRSAGKTGTTQSYRDAWYVGYTGNYVAAVWLGNDDYRPTDNMTGGTLPAMVWQRFMAYAHQ
NLELKPIPGIENPLPAKSDEAVAAAKTEGVEAGPRRPAHSLPLATRTVLNDLLGLFRQAPPVKPRRVSENLSAL

Sequences:

>Translated_714_residues
MVEKRTPKKGNTPRATIFLDIDAWIDSTLYELRFKAAEFWEDLTVFFRRFRFYGLRRAVIEFFSEGVTMLAAGSVVMLGL
AIPAFQETAGDWRAQDDYAVTFLDRYGQEIGQRGIRQRDSVPVDEMPDHVVKAVLATEDRRFFEHFGIDFVGLFRALSEN
MRANSVVQGGSTITQQLAKNLFLTNERTVERKIKEAFLAVWLEANLTKKEILQLYLDRAYLGGGTFGISAAADFYFGKQV
KDLTLPEAAMIAGLFKAPARYAPHINLPAARARANEVLSNLVQSRFMTEGQVLSARLNPATAVDRGDREAPDYFLDWAFE
EVKKIVPEGATHSLVARTTMDLDLQHAVEESIEFHLRQYGKDYDVEEGAAILMDTDGAVRALVGGRDYGQSQFNRATRAA
RQPGSSFKPYVYAAAMEAGLTPKTVVSDAPISWGNWSPQNYGRSYAGRVDLATALARSYNTVPVRLARDHLGIDVIQKLV
KDMGVETPLNGHKTMVLGTSDLTVLDQVTGYSVFANGGFAGTRHGILELHTHTGELVYDWRRDAPPPRRVLSETAVSNMN
SMLVEVPERGTARRAALPNIRSAGKTGTTQSYRDAWYVGYTGNYVAAVWLGNDDYRPTDNMTGGTLPAMVWQRFMAYAHQ
NLELKPIPGIENPLPAKSDEAVAAAKTEGVEAGPRRPAHSLPLATRTVLNDLLGLFRQAPPVKPRRVSENLSAL
>Mature_714_residues
MVEKRTPKKGNTPRATIFLDIDAWIDSTLYELRFKAAEFWEDLTVFFRRFRFYGLRRAVIEFFSEGVTMLAAGSVVMLGL
AIPAFQETAGDWRAQDDYAVTFLDRYGQEIGQRGIRQRDSVPVDEMPDHVVKAVLATEDRRFFEHFGIDFVGLFRALSEN
MRANSVVQGGSTITQQLAKNLFLTNERTVERKIKEAFLAVWLEANLTKKEILQLYLDRAYLGGGTFGISAAADFYFGKQV
KDLTLPEAAMIAGLFKAPARYAPHINLPAARARANEVLSNLVQSRFMTEGQVLSARLNPATAVDRGDREAPDYFLDWAFE
EVKKIVPEGATHSLVARTTMDLDLQHAVEESIEFHLRQYGKDYDVEEGAAILMDTDGAVRALVGGRDYGQSQFNRATRAA
RQPGSSFKPYVYAAAMEAGLTPKTVVSDAPISWGNWSPQNYGRSYAGRVDLATALARSYNTVPVRLARDHLGIDVIQKLV
KDMGVETPLNGHKTMVLGTSDLTVLDQVTGYSVFANGGFAGTRHGILELHTHTGELVYDWRRDAPPPRRVLSETAVSNMN
SMLVEVPERGTARRAALPNIRSAGKTGTTQSYRDAWYVGYTGNYVAAVWLGNDDYRPTDNMTGGTLPAMVWQRFMAYAHQ
NLELKPIPGIENPLPAKSDEAVAAAKTEGVEAGPRRPAHSLPLATRTVLNDLLGLFRQAPPVKPRRVSENLSAL

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=547, Percent_Identity=33.6380255941499, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI87082258, Length=312, Percent_Identity=39.7435897435897, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788867, Length=511, Percent_Identity=29.7455968688845, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1789601, Length=183, Percent_Identity=37.7049180327869, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79211; Mature: 79211

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKRTPKKGNTPRATIFLDIDAWIDSTLYELRFKAAEFWEDLTVFFRRFRFYGLRRAVI
CCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFFSEGVTMLAAGSVVMLGLAIPAFQETAGDWRAQDDYAVTFLDRYGQEIGQRGIRQRDS
HHHHCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCC
VPVDEMPDHVVKAVLATEDRRFFEHFGIDFVGLFRALSENMRANSVVQGGSTITQQLAKN
CCHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHECCCCHHHHHHHHHH
LFLTNERTVERKIKEAFLAVWLEANLTKKEILQLYLDRAYLGGGTFGISAAADFYFGKQV
HCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
KDLTLPEAAMIAGLFKAPARYAPHINLPAARARANEVLSNLVQSRFMTEGQVLSARLNPA
CCCCCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCC
TAVDRGDREAPDYFLDWAFEEVKKIVPEGATHSLVARTTMDLDLQHAVEESIEFHLRQYG
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
KDYDVEEGAAILMDTDGAVRALVGGRDYGQSQFNRATRAARQPGSSFKPYVYAAAMEAGL
CCCCCCCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCC
TPKTVVSDAPISWGNWSPQNYGRSYAGRVDLATALARSYNTVPVRLARDHLGIDVIQKLV
CCHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCEEEEHHHHCCHHHHHHHH
KDMGVETPLNGHKTMVLGTSDLTVLDQVTGYSVFANGGFAGTRHGILELHTHTGELVYDW
HHCCCCCCCCCCEEEEEECCCCHHHHHHCCCEEEECCCCCCCCCCEEEEEECCCCEEEEC
RRDAPPPRRVLSETAVSNMNSMLVEVPERGTARRAALPNIRSAGKTGTTQSYRDAWYVGY
CCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCHHHCCCCCCCCCCCCEEEEEE
TGNYVAAVWLGNDDYRPTDNMTGGTLPAMVWQRFMAYAHQNLELKPIPGIENPLPAKSDE
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCC
AVAAAKTEGVEAGPRRPAHSLPLATRTVLNDLLGLFRQAPPVKPRRVSENLSAL
CHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MVEKRTPKKGNTPRATIFLDIDAWIDSTLYELRFKAAEFWEDLTVFFRRFRFYGLRRAVI
CCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFFSEGVTMLAAGSVVMLGLAIPAFQETAGDWRAQDDYAVTFLDRYGQEIGQRGIRQRDS
HHHHCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCC
VPVDEMPDHVVKAVLATEDRRFFEHFGIDFVGLFRALSENMRANSVVQGGSTITQQLAKN
CCHHHCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHECCCCHHHHHHHHHH
LFLTNERTVERKIKEAFLAVWLEANLTKKEILQLYLDRAYLGGGTFGISAAADFYFGKQV
HCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
KDLTLPEAAMIAGLFKAPARYAPHINLPAARARANEVLSNLVQSRFMTEGQVLSARLNPA
CCCCCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCC
TAVDRGDREAPDYFLDWAFEEVKKIVPEGATHSLVARTTMDLDLQHAVEESIEFHLRQYG
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
KDYDVEEGAAILMDTDGAVRALVGGRDYGQSQFNRATRAARQPGSSFKPYVYAAAMEAGL
CCCCCCCCCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCC
TPKTVVSDAPISWGNWSPQNYGRSYAGRVDLATALARSYNTVPVRLARDHLGIDVIQKLV
CCHHHHCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCEEEEHHHHCCHHHHHHHH
KDMGVETPLNGHKTMVLGTSDLTVLDQVTGYSVFANGGFAGTRHGILELHTHTGELVYDW
HHCCCCCCCCCCEEEEEECCCCHHHHHHCCCEEEECCCCCCCCCCEEEEEECCCCEEEEC
RRDAPPPRRVLSETAVSNMNSMLVEVPERGTARRAALPNIRSAGKTGTTQSYRDAWYVGY
CCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCHHHCCCCCCCCCCCCEEEEEE
TGNYVAAVWLGNDDYRPTDNMTGGTLPAMVWQRFMAYAHQNLELKPIPGIENPLPAKSDE
CCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCC
AVAAAKTEGVEAGPRRPAHSLPLATRTVLNDLLGLFRQAPPVKPRRVSENLSAL
CHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]