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The map label for this gene is yiaO [H]

Identifier: 110633146

GI number: 110633146

Start: 867218

End: 868204

Strand: Reverse

Name: yiaO [H]

Synonym: Meso_0789

Alternate gene names: 110633146

Gene position: 868204-867218 (Counterclockwise)

Preceding gene: 110633153

Following gene: 110633145

Centisome position: 19.68

GC content: 59.98

Gene sequence:

>987_bases
ATGCTACGGAACATCACCAAATACATTGTTGCCGGCGCTACGGCCACAATGATGTTCCTCTCGCCGGCCACGGCGCAGGA
CAAGATTGTCATGCGCGTCGCGCATGCCATGCCGGAGTCCCACAGCTATCAGAAATGGGTCGAGAAGTTCCGCGACGAGC
TTGAAAAGCTCGTACCCGGACGCGTCGACGTGCAGATCTTCCCGAACGCCCAGCTCGGTAAGGAGACCGAATATATCGAA
GGCATGTCCTTCGGCACCATCGACGGCGCCGTCATGGGCCGACACGGCCAGATCGACCCGCGCCTCGATGTGTTGAACCT
GCCGATGATCTTCCGCGACGACGCCCACAAGGATGTCGTGATGCGCAGCGGTGGCGAGATCCAGAAGCAGCTCGACGACA
TCATGTACGAGAAAGGCTTCAAGGTCCTAGGCTGGGGCGAGCTCGGCTTCCGCTACATCACCACGAAAGACAAGCCCATC
AAGTCGGCCGCCGACCTGAAAGGCGTCGATATCCGCGTGCCGAATGTCGAGCCGTGGCTCGTTGCCTTCCGCGCCTGGGG
CGCCAACCCGACGCCGATGGACTTCTCCGAGCTCTATTCCGCGCTTCAGCAAGGTGTCGTGAGTGCGCAGGAAAATCCTC
CGGAAATCATCGAGACTTCGCGCTTCTATGAAGTGCAGAAATATCTCAGCCTGACGCGGCATGCGAGCATCCCGTCCCAG
TTCGTGGTCTCGCGCGCCTTCTGGGAAAAGCTTCCGGAAGACCTGCAGGCACCGGTCATGAAGGCAGCCACTATCAGCCG
GGACTACCAGGTTGCCCTCACCCGTGAGGCAAACAGCAGCCTGGTCTCAAAGCTGCAGGAACAGGGGATGGTCGTTATCG
AAGACGTCGATCGTGAATCCTTCCGCGCCGGCGCCGAGGAAGCCTACGCCAAGTATCAGGACATCATCGGCGCCGACCTT
ATCAAGGCGGTGCAAGAGGCGCACTGA

Upstream 100 bases:

>100_bases
TGTACCTCAGTATGCAAGGACACCTCCGCAGCGCCCGCGGAACCGGAGGTCATGGCGCTCAAATAACCGTCAACACAATT
CAATGGAGGAGACGTTCCAG

Downstream 100 bases:

>100_bases
TCATGACGCAAGTCTGCCGATTGCTGAGGGTAGCCGTCGGAGGTCTCATTGTCGCGCTCGTTTTGAGCGTGGCAATGGGC
GTCTTCTACCGATACGCCCT

Product: TRAP dicarboxylate transporter, DctP subunit

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MLRNITKYIVAGATATMMFLSPATAQDKIVMRVAHAMPESHSYQKWVEKFRDELEKLVPGRVDVQIFPNAQLGKETEYIE
GMSFGTIDGAVMGRHGQIDPRLDVLNLPMIFRDDAHKDVVMRSGGEIQKQLDDIMYEKGFKVLGWGELGFRYITTKDKPI
KSAADLKGVDIRVPNVEPWLVAFRAWGANPTPMDFSELYSALQQGVVSAQENPPEIIETSRFYEVQKYLSLTRHASIPSQ
FVVSRAFWEKLPEDLQAPVMKAATISRDYQVALTREANSSLVSKLQEQGMVVIEDVDRESFRAGAEEAYAKYQDIIGADL
IKAVQEAH

Sequences:

>Translated_328_residues
MLRNITKYIVAGATATMMFLSPATAQDKIVMRVAHAMPESHSYQKWVEKFRDELEKLVPGRVDVQIFPNAQLGKETEYIE
GMSFGTIDGAVMGRHGQIDPRLDVLNLPMIFRDDAHKDVVMRSGGEIQKQLDDIMYEKGFKVLGWGELGFRYITTKDKPI
KSAADLKGVDIRVPNVEPWLVAFRAWGANPTPMDFSELYSALQQGVVSAQENPPEIIETSRFYEVQKYLSLTRHASIPSQ
FVVSRAFWEKLPEDLQAPVMKAATISRDYQVALTREANSSLVSKLQEQGMVVIEDVDRESFRAGAEEAYAKYQDIIGADL
IKAVQEAH
>Mature_328_residues
MLRNITKYIVAGATATMMFLSPATAQDKIVMRVAHAMPESHSYQKWVEKFRDELEKLVPGRVDVQIFPNAQLGKETEYIE
GMSFGTIDGAVMGRHGQIDPRLDVLNLPMIFRDDAHKDVVMRSGGEIQKQLDDIMYEKGFKVLGWGELGFRYITTKDKPI
KSAADLKGVDIRVPNVEPWLVAFRAWGANPTPMDFSELYSALQQGVVSAQENPPEIIETSRFYEVQKYLSLTRHASIPSQ
FVVSRAFWEKLPEDLQAPVMKAATISRDYQVALTREANSSLVSKLQEQGMVVIEDVDRESFRAGAEEAYAKYQDIIGADL
IKAVQEAH

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=334, Percent_Identity=32.9341317365269, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36933; Mature: 36933

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRNITKYIVAGATATMMFLSPATAQDKIVMRVAHAMPESHSYQKWVEKFRDELEKLVPG
CCCHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
RVDVQIFPNAQLGKETEYIEGMSFGTIDGAVMGRHGQIDPRLDVLNLPMIFRDDAHKDVV
CEEEEECCCCCCCCCHHHHCCCCCCCCCCCEECCCCCCCCCCEEECCCEEECCCCCHHHH
MRSGGEIQKQLDDIMYEKGFKVLGWGELGFRYITTKDKPIKSAADLKGVDIRVPNVEPWL
HHCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHHCCCCCEEECCCCCHHH
VAFRAWGANPTPMDFSELYSALQQGVVSAQENPPEIIETSRFYEVQKYLSLTRHASIPSQ
HEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
FVVSRAFWEKLPEDLQAPVMKAATISRDYQVALTREANSSLVSKLQEQGMVVIEDVDRES
HHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEEECCHHH
FRAGAEEAYAKYQDIIGADLIKAVQEAH
HHCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRNITKYIVAGATATMMFLSPATAQDKIVMRVAHAMPESHSYQKWVEKFRDELEKLVPG
CCCHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
RVDVQIFPNAQLGKETEYIEGMSFGTIDGAVMGRHGQIDPRLDVLNLPMIFRDDAHKDVV
CEEEEECCCCCCCCCHHHHCCCCCCCCCCCEECCCCCCCCCCEEECCCEEECCCCCHHHH
MRSGGEIQKQLDDIMYEKGFKVLGWGELGFRYITTKDKPIKSAADLKGVDIRVPNVEPWL
HHCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCCHHHCCCCCEEECCCCCHHH
VAFRAWGANPTPMDFSELYSALQQGVVSAQENPPEIIETSRFYEVQKYLSLTRHASIPSQ
HEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
FVVSRAFWEKLPEDLQAPVMKAATISRDYQVALTREANSSLVSKLQEQGMVVIEDVDRES
HHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEEEECCHHH
FRAGAEEAYAKYQDIIGADLIKAVQEAH
HHCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]