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The map label for this gene is ispE

Identifier: 110633063

GI number: 110633063

Start: 780002

End: 780883

Strand: Reverse

Name: ispE

Synonym: Meso_0706

Alternate gene names: 110633063

Gene position: 780883-780002 (Counterclockwise)

Preceding gene: 110633064

Following gene: 110633062

Centisome position: 17.7

GC content: 60.77

Gene sequence:

>882_bases
ATGAAGCTGGAATGCATTGCTCCGGCAAAAATAAATCTTGCGCTGCACGTCATCGGCCGCCGGACTGACGGCTATCATCT
TCTGGAATCCCTTGTCGCCTTCGCTGCATTCGGCGACCGGCTCTCGGTCGAGAAGGCAGAAGCTGACACGTTTCTGTTGG
CAGGACCGTTCCGCGACGGGCTTGAGGGATCAGGCAATTTTGTCCTCAACGCGCGTGACCTCCTCAGAGAAAAGTTCGCA
AGCCGGGCGAGCAGTCCCGTCGCCATCACATTGGAGAAGAACCTGCCGGTGGCATCAGGAATCGGCGGCGGGTCGAGTGA
TGCGGCGACGGGGCTCAAGCTCCTGACGCGTTATTGGGAAATCGAAACCGAACCGGCCGAACTTGCGCAGATCGGGCTGG
GGCTTGGAGCCGACGTTCCGATGTGCCTTTTTGGCCAGCCGCTGATCGCAAGCGGCATCGGCGAGAAGCTGGAGCCGGTT
TCAACGCTCCCTTCTCTTCCCATCCTTCTTGTCAATCCCGGCCTTCCGTTGGCGACGCCCGCTGTTTTCTCGTCGCTTGT
CGAGCGCGAGAATGCACCTCTCCCGCCTGCTCCGCGCAATAAGAGCGCCGCCGAGCTTGTAAATTGGCTCAGGCTTACCC
GAAACGATCTCGAACCGGCGGCACTTTCAATCATGCCGGTTATCGGCATCGTGCTCAGACTTCTGCAGACGGAGGGCGCA
CTCATCGCCCGGATGTCCGGCTCCGGGGCCACCTGCTTCGGGATTTTCGAAACCGCGAGCAAAGCCCGGCAGGCGGGCGA
GATGATCTCCCGGAGACATCCCGGCTGGTTTGTCCGCGCGACCCAGACGAGGGCCTCCATGCCGGAGTTGAGTCATGTCT
GA

Upstream 100 bases:

>100_bases
CGTGTCCGTAAGGAGACAGCCACCGGCAGCATGGGCACGCTCGTCAAGGATCCGAGGACCGGTGAATATCGCCTGGCCAA
GGACTGAGCGCGCCCCTGAC

Downstream 100 bases:

>100_bases
ACGGCAGTTCATTCCCGTGCGATTCGCGGTGCTCACCATTTCCGACACGCGCACCATGGAGAACGACAAGTCCGGCCAGA
CCTTGGCTGATCGCATAGAG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKLECIAPAKINLALHVIGRRTDGYHLLESLVAFAAFGDRLSVEKAEADTFLLAGPFRDGLEGSGNFVLNARDLLREKFA
SRASSPVAITLEKNLPVASGIGGGSSDAATGLKLLTRYWEIETEPAELAQIGLGLGADVPMCLFGQPLIASGIGEKLEPV
STLPSLPILLVNPGLPLATPAVFSSLVERENAPLPPAPRNKSAAELVNWLRLTRNDLEPAALSIMPVIGIVLRLLQTEGA
LIARMSGSGATCFGIFETASKARQAGEMISRRHPGWFVRATQTRASMPELSHV

Sequences:

>Translated_293_residues
MKLECIAPAKINLALHVIGRRTDGYHLLESLVAFAAFGDRLSVEKAEADTFLLAGPFRDGLEGSGNFVLNARDLLREKFA
SRASSPVAITLEKNLPVASGIGGGSSDAATGLKLLTRYWEIETEPAELAQIGLGLGADVPMCLFGQPLIASGIGEKLEPV
STLPSLPILLVNPGLPLATPAVFSSLVERENAPLPPAPRNKSAAELVNWLRLTRNDLEPAALSIMPVIGIVLRLLQTEGA
LIARMSGSGATCFGIFETASKARQAGEMISRRHPGWFVRATQTRASMPELSHV
>Mature_293_residues
MKLECIAPAKINLALHVIGRRTDGYHLLESLVAFAAFGDRLSVEKAEADTFLLAGPFRDGLEGSGNFVLNARDLLREKFA
SRASSPVAITLEKNLPVASGIGGGSSDAATGLKLLTRYWEIETEPAELAQIGLGLGADVPMCLFGQPLIASGIGEKLEPV
STLPSLPILLVNPGLPLATPAVFSSLVERENAPLPPAPRNKSAAELVNWLRLTRNDLEPAALSIMPVIGIVLRLLQTEGA
LIARMSGSGATCFGIFETASKARQAGEMISRRHPGWFVRATQTRASMPELSHV

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=282, Percent_Identity=34.7517730496454, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_MESSB (Q11KG9)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673271.1
- ProteinModelPortal:   Q11KG9
- SMR:   Q11KG9
- STRING:   Q11KG9
- GeneID:   4180613
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_0706
- NMPDR:   fig|266779.1.peg.2843
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- PhylomeDB:   Q11KG9
- ProtClustDB:   PRK14608
- BioCyc:   MSP266779:MESO_0706-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31151; Mature: 31151

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLECIAPAKINLALHVIGRRTDGYHLLESLVAFAAFGDRLSVEKAEADTFLLAGPFRDG
CCCEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
LEGSGNFVLNARDLLREKFASRASSPVAITLEKNLPVASGIGGGSSDAATGLKLLTRYWE
CCCCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHC
IETEPAELAQIGLGLGADVPMCLFGQPLIASGIGEKLEPVSTLPSLPILLVNPGLPLATP
CCCCHHHHHHHHCCCCCCCCHHHCCCHHHHHCCCCCCCHHHHCCCCCEEEECCCCCCCCH
AVFSSLVERENAPLPPAPRNKSAAELVNWLRLTRNDLEPAALSIMPVIGIVLRLLQTEGA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
LIARMSGSGATCFGIFETASKARQAGEMISRRHPGWFVRATQTRASMPELSHV
EEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHCCC
>Mature Secondary Structure
MKLECIAPAKINLALHVIGRRTDGYHLLESLVAFAAFGDRLSVEKAEADTFLLAGPFRDG
CCCEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
LEGSGNFVLNARDLLREKFASRASSPVAITLEKNLPVASGIGGGSSDAATGLKLLTRYWE
CCCCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHC
IETEPAELAQIGLGLGADVPMCLFGQPLIASGIGEKLEPVSTLPSLPILLVNPGLPLATP
CCCCHHHHHHHHCCCCCCCCHHHCCCHHHHHCCCCCCCHHHHCCCCCEEEECCCCCCCCH
AVFSSLVERENAPLPPAPRNKSAAELVNWLRLTRNDLEPAALSIMPVIGIVLRLLQTEGA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
LIARMSGSGATCFGIFETASKARQAGEMISRRHPGWFVRATQTRASMPELSHV
EEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA