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The map label for this gene is appA [H]

Identifier: 110632922

GI number: 110632922

Start: 621642

End: 623252

Strand: Reverse

Name: appA [H]

Synonym: Meso_0563

Alternate gene names: 110632922

Gene position: 623252-621642 (Counterclockwise)

Preceding gene: 110632923

Following gene: 110632921

Centisome position: 14.12

GC content: 54.38

Gene sequence:

>1611_bases
ATGCGCATTCAAATGGGAATGAAAGCAAAGGCGGCGTTCATAACTATCGGGGCAATAATTACCGTCGCCAACAGCCTAAG
CAACGCCTCCGCTCAGACGAGCGGAGGAGATCTGGTCACCGTAATGCGGGCGGATCCAAAAGGTCTTATACCTGCATTTC
CCCACGGAGGTGACACTCGGATCATCGCAGCAAAGATCTTTGAGGGCCTGATATCCTACAGCACGGATTTAAGGCCGCTC
CCAGGGCTCGCCAAGAGCTGGGAAATCTCGAGCGATGGCCTGACCTATACGTTCAAACTCCAAAACGGCGTGCATTGGAA
CGATGGCAAGGAGTTCTCGTCGGCCGACGTCCTATTCAGTCTGAACGAAATGCTTCCCGCCACCAATGCGGCGGCGAAGC
AGGCGCTTGAAGAGGTGAAATCCATTGAGGCGCCCGACGCGGAAACGGTCGTGATCACCCTCAAGCGGCGCGTGCCCTAC
TTCCTTATGCTCTTCCCGGGCCAACAGATGCCCATCATGCCTAAGCATATCTATGAGGGCACCGACTACATAAAGAATCC
AGCGAATCTAATGCCAATCGGCACGGGTCCATTCGAGTTGGTCGAATGGAAGCGTGGTTCCTACGTTCGTCTTGAGAAGA
ACGAGAATTACTGGAAGAAGCCGGAACCCTACCTGGATTCAATCACCTATCGGATCATGCCGGATGCATCTTCGCGCGCG
ATCGCCCTGGAAAGTGGCGATGTCAATGTCGCGACGTCGGGAGAATTGAGCAGTTCGGACATTGCGCGCTTCAAGACATC
CGGCGAGTTTCGGATGGATTCCAAAGGTTGGGAATATTTCGCCAGCCAGGCGCTTCTGGTCATCAATGTGCGGAACCACG
TTCTACAGGATGCCCGGTTCAGACAGGCACTCTCCTATGCACTCGACAAGAGATTCATCCTAGATCACGTGTGGAACGGC
ACCGGTGCAATTTCGACCGGGCCCGTTAGCCACTCTATCCCGTTCTATACCGATAAAATTTCGAGCTACAGCTTCGACCC
CAAGAAAGCGAGCGCCTTGCTCGACGAGATTGGGTTGAGGGCAGGTGCCGACGACAAGAGGCTCGATGCCCAAGGCAATC
CGATTACTGTTAAACTGCTGATATTGCCCGGAGAACCTTATGGCAGGTTGGCGGAGTATATTAGGGAGGCGTGGCGCGGC
ATTGGCATCGCCACGGATCTGCAGGCAGTCGACCTAGGCAACTGGTATGACCGCATGGCCAACTGGAACTTCGATGTCGC
CATCGCAAATCTAGGCCAATTTGCCGATCCCGGGATCGGAGCTAGGAGCTACTATCATTCGGGCAACATCAAAAAAGGTG
TGATGTTCTCGAACATGGGCGGCTACAAGAACGGGGATGTCGACGCACTGTTTGATCAGGCTTCACTCGAGATGGATCCT
GAACGCCGCGGCGAGATCTATCACCAGATCCAGCAGAAGATCGTCACTGATGCTCCCGCAATCTGGTTAATTGACCTCGA
GCGCTTCACCGTCAGCCAAAAGAATGTTCACAACCTGGTGACGGACGCGTTCGGACCATGGGGTTCATGGGCAAGCGCCT
ACATTGAATAG

Upstream 100 bases:

>100_bases
AAAATACCTGCGCTGAGGCTGATGGATGATCGGTCACTTCAAGGAGAAAAAAGGTCTAGTTATCCAATGAGTTAGAATAA
CTAAAAACAGGGGATTCGCA

Downstream 100 bases:

>100_bases
TGATCAGCTATGCAGTTTCTCGGTTACGTCTCCTGGCGCCTGCTGAAGGCTGCCGCTTTGCTGGTGGCCGTGATATCCTT
GAATTTCCTGTTGATCAGGA

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MRIQMGMKAKAAFITIGAIITVANSLSNASAQTSGGDLVTVMRADPKGLIPAFPHGGDTRIIAAKIFEGLISYSTDLRPL
PGLAKSWEISSDGLTYTFKLQNGVHWNDGKEFSSADVLFSLNEMLPATNAAAKQALEEVKSIEAPDAETVVITLKRRVPY
FLMLFPGQQMPIMPKHIYEGTDYIKNPANLMPIGTGPFELVEWKRGSYVRLEKNENYWKKPEPYLDSITYRIMPDASSRA
IALESGDVNVATSGELSSSDIARFKTSGEFRMDSKGWEYFASQALLVINVRNHVLQDARFRQALSYALDKRFILDHVWNG
TGAISTGPVSHSIPFYTDKISSYSFDPKKASALLDEIGLRAGADDKRLDAQGNPITVKLLILPGEPYGRLAEYIREAWRG
IGIATDLQAVDLGNWYDRMANWNFDVAIANLGQFADPGIGARSYYHSGNIKKGVMFSNMGGYKNGDVDALFDQASLEMDP
ERRGEIYHQIQQKIVTDAPAIWLIDLERFTVSQKNVHNLVTDAFGPWGSWASAYIE

Sequences:

>Translated_536_residues
MRIQMGMKAKAAFITIGAIITVANSLSNASAQTSGGDLVTVMRADPKGLIPAFPHGGDTRIIAAKIFEGLISYSTDLRPL
PGLAKSWEISSDGLTYTFKLQNGVHWNDGKEFSSADVLFSLNEMLPATNAAAKQALEEVKSIEAPDAETVVITLKRRVPY
FLMLFPGQQMPIMPKHIYEGTDYIKNPANLMPIGTGPFELVEWKRGSYVRLEKNENYWKKPEPYLDSITYRIMPDASSRA
IALESGDVNVATSGELSSSDIARFKTSGEFRMDSKGWEYFASQALLVINVRNHVLQDARFRQALSYALDKRFILDHVWNG
TGAISTGPVSHSIPFYTDKISSYSFDPKKASALLDEIGLRAGADDKRLDAQGNPITVKLLILPGEPYGRLAEYIREAWRG
IGIATDLQAVDLGNWYDRMANWNFDVAIANLGQFADPGIGARSYYHSGNIKKGVMFSNMGGYKNGDVDALFDQASLEMDP
ERRGEIYHQIQQKIVTDAPAIWLIDLERFTVSQKNVHNLVTDAFGPWGSWASAYIE
>Mature_536_residues
MRIQMGMKAKAAFITIGAIITVANSLSNASAQTSGGDLVTVMRADPKGLIPAFPHGGDTRIIAAKIFEGLISYSTDLRPL
PGLAKSWEISSDGLTYTFKLQNGVHWNDGKEFSSADVLFSLNEMLPATNAAAKQALEEVKSIEAPDAETVVITLKRRVPY
FLMLFPGQQMPIMPKHIYEGTDYIKNPANLMPIGTGPFELVEWKRGSYVRLEKNENYWKKPEPYLDSITYRIMPDASSRA
IALESGDVNVATSGELSSSDIARFKTSGEFRMDSKGWEYFASQALLVINVRNHVLQDARFRQALSYALDKRFILDHVWNG
TGAISTGPVSHSIPFYTDKISSYSFDPKKASALLDEIGLRAGADDKRLDAQGNPITVKLLILPGEPYGRLAEYIREAWRG
IGIATDLQAVDLGNWYDRMANWNFDVAIANLGQFADPGIGARSYYHSGNIKKGVMFSNMGGYKNGDVDALFDQASLEMDP
ERRGEIYHQIQQKIVTDAPAIWLIDLERFTVSQKNVHNLVTDAFGPWGSWASAYIE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=559, Percent_Identity=27.3703041144902, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1787052, Length=511, Percent_Identity=25.440313111546, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1789887, Length=423, Percent_Identity=28.1323877068558, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787762, Length=524, Percent_Identity=24.618320610687, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081878, Length=520, Percent_Identity=24.8076923076923, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787495, Length=478, Percent_Identity=25.5230125523013, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1789397, Length=491, Percent_Identity=23.4215885947047, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787551, Length=319, Percent_Identity=20.6896551724138, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786650, Length=187, Percent_Identity=27.807486631016, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59376; Mature: 59376

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIQMGMKAKAAFITIGAIITVANSLSNASAQTSGGDLVTVMRADPKGLIPAFPHGGDTR
CEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCE
IIAAKIFEGLISYSTDLRPLPGLAKSWEISSDGLTYTFKLQNGVHWNDGKEFSSADVLFS
EEHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEEEEECCCEECCCCCCCCCCHHEEE
LNEMLPATNAAAKQALEEVKSIEAPDAETVVITLKRRVPYFLMLFPGQQMPIMPKHIYEG
HHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHCC
TDYIKNPANLMPIGTGPFELVEWKRGSYVRLEKNENYWKKPEPYLDSITYRIMPDASSRA
CHHHCCCCCEEECCCCCHHHEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCE
IALESGDVNVATSGELSSSDIARFKTSGEFRMDSKGWEYFASQALLVINVRNHVLQDARF
EEEECCCEEEEECCCCCCCHHHHHCCCCCEEECCCCHHHHHCCEEEEEECHHHHHHHHHH
RQALSYALDKRFILDHVWNGTGAISTGPVSHSIPFYTDKISSYSFDPKKASALLDEIGLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCC
AGADDKRLDAQGNPITVKLLILPGEPYGRLAEYIREAWRGIGIATDLQAVDLGNWYDRMA
CCCCCCEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCEECCCEEEECCHHHHHHC
NWNFDVAIANLGQFADPGIGARSYYHSGNIKKGVMFSNMGGYKNGDVDALFDQASLEMDP
CCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCH
ERRGEIYHQIQQKIVTDAPAIWLIDLERFTVSQKNVHNLVTDAFGPWGSWASAYIE
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure
MRIQMGMKAKAAFITIGAIITVANSLSNASAQTSGGDLVTVMRADPKGLIPAFPHGGDTR
CEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCE
IIAAKIFEGLISYSTDLRPLPGLAKSWEISSDGLTYTFKLQNGVHWNDGKEFSSADVLFS
EEHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEEEEECCCEECCCCCCCCCCHHEEE
LNEMLPATNAAAKQALEEVKSIEAPDAETVVITLKRRVPYFLMLFPGQQMPIMPKHIYEG
HHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHCC
TDYIKNPANLMPIGTGPFELVEWKRGSYVRLEKNENYWKKPEPYLDSITYRIMPDASSRA
CHHHCCCCCEEECCCCCHHHEEECCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCE
IALESGDVNVATSGELSSSDIARFKTSGEFRMDSKGWEYFASQALLVINVRNHVLQDARF
EEEECCCEEEEECCCCCCCHHHHHCCCCCEEECCCCHHHHHCCEEEEEECHHHHHHHHHH
RQALSYALDKRFILDHVWNGTGAISTGPVSHSIPFYTDKISSYSFDPKKASALLDEIGLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCC
AGADDKRLDAQGNPITVKLLILPGEPYGRLAEYIREAWRGIGIATDLQAVDLGNWYDRMA
CCCCCCEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCEECCCEEEECCHHHHHHC
NWNFDVAIANLGQFADPGIGARSYYHSGNIKKGVMFSNMGGYKNGDVDALFDQASLEMDP
CCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCCCCCHHHHCCCCCCCCCH
ERRGEIYHQIQQKIVTDAPAIWLIDLERFTVSQKNVHNLVTDAFGPWGSWASAYIE
HHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]